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Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

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At least 19 records
All sourcesNASA NTRSDOE OSTI
NASA NTRS2009

Distribution of Chromosome Breakpoints in Human Epithelial Cells Exposed to Low- and High-LET Radiations

The advantage of the multicolor banding in situ hybridization (mBAND) technique is not only its ability to identify simultaneously both inter- and intrachromosome exchanges, but also the ability to measure the breakpoint location along the length of the chromosome in a precision that is unmatched with other traditional banding techniques. Breakpoints on specific regions of a chromosome have been known to associate with specific cancers. The breakpoint distribution in cells after low- and high-LET radiation exposures will also provide the data for biophysical modeling of the chromatin structure, as well as the data for the modeling the formation of radiation-induced chromosome aberrations. In a series of experiments, we studied low- and high-LET radiation-induced chromosome aberrations using the mBAND technique with chromosome 3 painted in 23 different colored bands. Human epithelial cells (CH1 84B5F5/M10) were exposed in vitro to Cs- 137 rays at both low and high dose rates, secondary neutrons with a broad energy spectrum at a low dose rate and 600 MeV/u Fe ions at a high dose rate. The data of both inter- and intrachromosome aberrations involving the painted chromosome have been reported previously. Here we present data of the location of the chromosome breaks along the length of chromosome 3 in the cells after exposures to each of the four radiation scenarios. In comparison to the expected breakpoint distribution based on the length of the bands, the observed distribution appeared to be non-random for both the low- and high-LET radiations. In particular, hot spots towards both ends of the chromosome were found after low-LET irradiations of either low or high dose rates. For both high-LET radiation types (Fe ions and neutrons), the breakpoint distributions were similar, and were much smoother than that for low-LET radiation. The dependence of the breakpoint distribution on the radiation quality requires further investigations.

Hada, Megumi↗
NASA NTRS1995

Radial evolution of the high/low frequency breakpoint in magnetic field spectra

The spectra of magnetic field variations in the solar wind show different behavior in two frequency regions; a high frequency region in which the spectral exponent is about -5/3 and a low frequency region in which it is typically -1. The two types of variations must arise from different processes and a clue to the relationship between the spectral regions lies in understanding the behavior of the breakpoint between the spectral regions. Studies of the average behavior of spectra have shown that the break point occurs at about 3.5 hours at 1 AU. It is also known that, on average, the breakpoint occurs at lower frequencies with larger heliocentric distances. Ideally however, instead of the average properties of the spectra, we would like to know how the breakpoint evolves in particular samples of the solar wind as they propagate to larger heliocentric distances. In the study reported here we take advantage of the fact that, in 1974, Pioneer 10 (4.4 AU) and Pioneer 11 (5.6 AU) were close to being co-aligned and being aligned with the Earth. Solar wind observed at Earth can be closely matched with solar wind later observed at P10 and P11. We here compare the breakpoint observed at Earth with that observed at Pioneers 10 and 11 for matched samples of the wind.

Feynman, J.↗
NASA NTRS2010

Distribution of Chromosome Breakpoints in Human Epithelial Cells Exposed to Low- and High-LET Radiation

Low-and high-LET radiations produced distinct breakpoint distributions. The difference of the breakpoint distributions between low-and high-LET only appeared in break ends involved in interchromosome exchanges. The breakpoint distributions for break ends participating in intrachromosome exchanges were similar. Gene-rich regions do not necessarily have more chromosome breaks. High-LET appeared to produce long live (data not shown) or longer live breaks that can migrate a longer distance before rejoining with other breaks. Domains occupied by different segments of the chromosomes may be responsible for the breakpoint distribution. The dose responses for interchromosomal exchanges were linear in all four exposures. However, the dose response for intrachromosomal exchanges were none linear. Increasing dose of high dose rate exposure (Fe-ions or -rays) increase the fraction of cells with intrachromosome aberrations, whereas increasing dose of low dose rate exposure (neutrons or -rays) does not affect the fraction of cells with intrachromosome aberrations.

Hada, Megumi↗
NASA NTRS2015

Proximity Within Interphase Chromosome Contributes to the Breakpoint Distribution in Radiation-Induced Intrachromosomal Exchanges

Previously, we reported that breaks involved in chromosome aberrations were clustered in several regions of chromosome3 in human mammary epithelial cells after exposures to either low-or high-LET radiation. In particular, breaks in certain regions of the chromosome tended to rejoin with each other to form an intrachromosome exchange event. This study tests the hypothesis that proximity within a single chromosome in interphase cell nuclei contributes to the distribution of radiation-induced chromosome breaks. Chromosome 3 in G1 human mammary epithelial cells was hybridized with the multicolor banding in situ hybridization (mBAND) probes that distinguish the chromosome in six differently colored regions, and the location of these regions was measured with a laser confocal microscope. Results of the study indicated that, on a multi-mega base pair scale of the DNA, the arrangement of chromatin was non-random. Both telomere regions tended to be located towards the exterior of the chromosome domain, whereas the centromere region towards the interior. In addition, the interior of the chromosome domain was preferentially occupied by the p-arm of the chromatin, which is consistent with our previous finding of intrachromosome exchanges involving breaks on the p-arm and in the centromere region of chromosome3. Other factors, such as the fragile sites in the 3p21 band and gene regulation, may also contribute to the breakpoint distribution in radiation-induced chromosome aberrations. Further investigations suggest that the 3D chromosome folding is cell type and culture condition dependent.

Zhang, Ye↗
NASA NTRS2022

Detecting the Greatest Changes in Global Satellite-Based Precipitation Observations

In recent years, the analysis of abrupt and non-abrupt changes in precipitation has received much attention due to the importance of climate change-related issues (e.g., extreme climate events). In this study, we used a novel segmentation algorithm, DBEST (Detecting Breakpoints and Estimating Segments in Trend), to analyze the greatest changes in precipitation using a monthly pixel-based satellite precipitation dataset (TRMM 3B43) at three different scales: (i) global, (ii) continental, and (iii) climate zone, during the 1998–2019 period. We found significant breakpoints, 14.1%, both in the form of abrupt and non-abrupt changes, in the global scale precipitation at the 0.05 significance level. Most of the abrupt changes were observed near the Equator in the Pacific Ocean and Asian continent, relative to the rest of the globe. Most detected breakpoints occurred during the 1998–1999 and 2009–2011 periods on the global scale. The average precipitation change for the detected breakpoint was ±100 mm, with some regions reaching ±3000 mm. For instance, most portions of northern Africa and Asia experienced major changes of approximately +100 mm. In contrast, most of the South Pacific and South Atlantic Ocean experienced changes of −100 mm during the studied period. Our findings indicated that the larger areas of Africa (23.9%), Asia (22.9%), and Australia (15.4%) experienced significant precipitation breakpoints compared to North America (11.6%), South America (9.3%), Europe (8.3%), and Oceania (9.6%). Furthermore, we found that the majority of detected significant breakpoints occurred in the arid (31.6%) and polar (24.1%) climate zones, while the least significant breakpoints were found for snow-covered (11.5%), equatorial (7.5%), and warm temperate (7.7%) climate zones. Positive breakpoints’ temporal coverage in the arid (54.0%) and equatorial (51.9%) climates were more than those in other climates zones. Here, the findings indicated that large areas of Africa and Asia experienced significant changes in precipitation (−250 to +250 mm). Compared to the average state (trend during a specific period), the greatest changes in precipitation were more abrupt and unpredictable, which might impose a severe threat to the ecology, environment, and natural resources.

Breakpoint↗
NASA NTRS2018

Vegetation Change Detection in Southern California Solar Energy Developments

Change detection from satellite sensor vegetation indices (VIs) presents an opportunity to monitor trends and disturbances at the regional scale for southern California's Mojave and Lower Colorado Deserts. Renewable energy sites are being constructed in this region on public lands under the Bureau of Land Management (BLM). We have developed a framework for VI change detection over the past two decades, with initial focus on three sites, Joshua Tree National Park, Mojave National Preserve, and a proximal group of Development Focus Areas (DFAs), for comparison between protected and development-targeted lands. Three Terra MODIS VIs (normalized difference [NDVI], enhanced [EVI], soil-adjusted [SAVI]) were evaluated in the Breaks for Additive Season and Trend (BFAST) setting for the regional MODIS 250-m resolution grid to estimate significant time series shifts (breakpoints) from February 2000 to May 2018. All three VIs tended to detect the maximum number of breakpoints at a grid location, but cross-correlations with precipitation and comparison with timing of wildfire burns near the study sites for breakpoint density (proportion of area with a breakpoint) showed that NDVI had the strongest response to these major disturbances, supporting its use for subsequent analysis. Time series of NDVI breakpoint change densities for individual solar energy sites did not have a consistent vegetation response following construction. Bootstrapping showed that the DFAs had significantly larger kurtosis and variance in the positive NDVI breakpoint distribution than did the protected sites, but there was no significant difference in the negative distribution for all three sites. The inconsistent post-construction NDVI signal and the large number of breakpoints overall suggested that the largest changes in vegetation cover density were tied to seasonal precipitation amounts. The distributional results indicated that existing site-specific conditions were the main control on VI responses, given the history of human disturbances in the DFAs. Although the results do not support persistent VI disturbances resulting from recent solar energy development, continued monitoring and examination of other ecological variables and surface temperatures will be vital to the long-term protection of this desert environment.

Potter, Christopher S.↗
NASA NTRS1977

Thermal stability of some aircraft turbine fuels derived from oil shale and coal

Thermal stability breakpoint temperatures are shown for 32 jet fuels prepared from oil shale and coal syncrudes by various degrees of hydrogenation. Low severity hydrotreated shale oils, with nitrogen contents of 0.1 to 0.24 weight percent, had breakpoint temperatures in the 477 to 505 K (400 to 450 F) range. Higher severity treatment, lowering nitrogen levels to 0.008 to 0.017 weight percent, resulted in breakpoint temperatures in the 505 to 533 K (450 to 500 F) range. Coal derived fuels showed generally increasing breakpoint temperatures with increasing weight percent hydrogen, fuels below 13 weight percent hydrogen having breakpoints below 533 K (500 F). Comparisons are shown with similar literature data.

Reynolds, T. W.↗
NASA NTRS1980

Determination of jet fuel thermal deposit rate using a modified JFTOT

Three fuels having different breakpoint temperatures were studied in the modified jet fuel thermal oxidation tester. The lower stability fuel with a breakpoint of 240 C was first stressed at a constant temperature. After repeating this procedure at several different temperatures, an Arrehenius plot was drawn from the data. The correlation coefficient and the energy of activation were calculated to be 0.97 and 8 kcal/mole respectively. Two other fuels having breakpoint temperatures of 271 C and 285 C were also studied in a similar manner. A straight line was drawn through the data at a slope equivalent to the slope of the lower stability fuel. The deposit formation rates for the three fuels were determined at 260 C, and a relative deposit formation rate at this temperature was calculated and plotted as a function of the individual fuel's breakpoint temperatures.

Nowack, C. J.↗
DOE OSTI2020

Nonlinear drought plasticity reveals intraspecific diversity in a dominant grass species

Abstract Intraspecific diversity of dominant species in native plant communities can modulate ecosystem function under both optimal and stressful conditions. Yet, few genotype by environment interaction studies quantify differences in the shape of plasticity functions or phenotypic breakpoints across genotypes in natural populations. Using three genotypes with a history of drought selection, we performed a greenhouse study on the dominant tallgrass prairie species Andropogon gerardii . We investigated phenotypic plasticity and recovery differences among genotypes across a water availability gradient, measuring growth‐related, instantaneous and cumulative phenotypes. To further understand genotype by environment effects, we quantified plasticity functions and breakpoints among genotypes. Like other studies, we found strong evidence for phenotypic and plasticity differences among genotypes. However, we also found nonlinear plasticity functions and breakpoints were common across phenotypes, especially relative growth rates, biomass allocation and root architecture. Drought selected genotypes were also more likely to flower during recovery, but all genotypes were resilient to drought across treatments. We demonstrate that plasticity functions may help explain intraspecific diversity, patterns of selection and nonlinear community responses to more variable rainfall within an experimental population. In particular, plasticity functions can help disentangle drought/variability tolerance versus acquisitive strategies. A better understanding of intraspecific diversity in this grass species will provide more mechanistic insight into its ability to buffer ecosystem changes and provide resiliency in the tallgrass prairie under future droughts. A free Plain Language Summary can be found within the Supporting Information of this article.

Hoffman, Ava M.↗
DOE OSTI2023

Comprehensive Genetic Characterization of Four Novel HIV-1 Circulating Recombinant Forms (CRF129_56G, CRF130_A1B, CRF131_A1B, and CRF138_cpx): Insights from Molecular Epidemiology in Cyprus

Molecular investigations of the HIV-1 pol region (2253–5250 in the HXB2 genome) were conducted on sequences obtained from 331 individuals infected with HIV-1 in Cyprus between 2017 and 2021. This study unveiled four distinct HIV-1 putative transmission clusters, encompassing 19 previously unidentified HIV-1 recombinants. These recombinants, each comprising eight, three, four, and four sequences, respectively, did not align with previously established Circulating Recombinant Forms (CRFs). To characterize these novel HIV-1 recombinants, near-full-length genome sequences were successfully obtained for 16 of the 19 recombinants (790–8795 in the HXB2 genome) using an in-house-developed RT-PCR assay. Phylogenetic analyses, employing MEGAX and Cluster-Picker, along with confirmatory neighbor-joining tree analyses of subregions, were conducted to identify distinct clusters and determine subtypes. The uniqueness of the HIV-1 recombinants was evident in their exclusive clustering within generated maximum likelihood trees. Recombination analyses highlighted the distinct chimeric nature of these recombinants, with consistent mosaic patterns observed across all sequences within each of the four putative transmission clusters. Conclusive genetic characterization identified four novel HIV-1 CRFs: CRF129_56G, CRF130_A1B, CRF131_A1B, and CRF138_cpx. CRF129_56G exhibited two recombination breakpoints and three fragments of subtypes CRF56_cpx and G. Both CRF130_A1B and CRF131_A1B featured seven recombination breakpoints and eight fragments of subtypes A1 and B. CRF138_cpx displayed five recombination breakpoints and six fragments of subtypes CRF22_01A1 and F2, along with an unclassified fragment. Additional BLAST analyses identified a Unique Recombinant Form (URF) of CRF138_cpx with three additional recombination sites, involving subtype F2, a fragment of unknown subtype origin, and CRF138_cpx. Post-identification, all putative transmission clusters remained active, with CRF130_A1B, CRF131_A1B, and CRF138_cpx clusters exhibiting further growth. Furthermore, international connections were identified through BLAST analyses, linking one sequence from the USA to the CRF130_A1B strain, and three sequences from Belgium and Cameroon to the CRF138_cpx strain. This study contributes valuable insights into the dynamic landscape of HIV-1 diversity and transmission patterns, emphasizing the need for ongoing molecular surveillance and global collaboration in tracking emerging viral variants.

60 APPLIED LIFE SCIENCES↗
NASA NTRS2010

Distributions of Low- and High-LET Radiation-Induced Breaks in Chromosomes are Associated with Inter- and Intrachromosome Exchanges

To study the breakpoint along the length of the chromosome induced by low- and high-LET radiations, we exposed human epithelial cells in vitro to Cs-137 rays at both low and high dose rates, secondary neutrons at a low dose rate, and 600 MeV/u Fe ions at a high dose rate. The location of the breaks was identified using the multicolor banding in situ hybridization (mBAND) that paints Chromosome 3 in 23 different colored bands. The breakpoint distributions were found to be similar between rays of low and high dose rates and between the two high-LET radiation types. Detailed analysis of the chromosome break ends involved in inter- and intrachromosome exchanges revealed that only the break ends participating in interchromosome exchanges contributed to the hot spots found for low-LET. For break ends participating in intrachromosome exchanges, the distributions for all four radiation scenarios were similar with clusters of breaks found in three regions. Analysis of the locations of the two break ends in Chromosome 3 that joined to form an intrachromosome exchange demonstrated that two breaks with a greater genomic separation may be more likely to rejoin than two closer breaks, indicating that chromatin folding can play an important role in the rejoining of chromosome breaks. Our study demonstrated that the gene-rich regions do not necessarily contain more breaks. The breakpoint distribution depends more on the likelihood that a break will join with another break in the same chromosome or in a different chromosome.

Hada, Megumi↗
NASA NTRS2010

Dissipation of Turbulence in the Solar Wind

I will describe the first three-dimensional (3-D) dispersion relations and wavenumber spectra of magnetic turbulence in the solar wind at sub-proton scales. The analysis takes advantage of the short separations of the Cluster spacecraft (d/sim approx.200 km) to apply the {it k}-filtering technique to the frequency range where the transition to sub-proton scales occurs. The dispersion diagrams show unambiguously that the cascade is carried by highly oblique Kinetic Alfven Wave with \omega\leq 0.1\omega_{ci} in the plasma rest frame down to k_\perp\rho_i \sim 2. The wavenumber spectra in the direction perpendicular to the mean magnetic field consists of two ranges of scales separated by a breakpoint in the interval [0.4,1] k_\perp \rho_i. Above the breakpoint, the spectra follow the Kolmogorov scaling k_\perp^{-1.7}, consistent with existing theoretical predictions. Below the breakpoint, the spectra steepen to \sim k_\perp^{-4.5}. We conjecture that the turbulence undergoes a {\it transition-range}, where part of energy is dissipated into proton heating via Landau damping, and the remaining energy cascades down to electron scales where electron Landau damping may predominate.

Goldstein, Melvyn L.↗
NASA NTRS2013

Trend Change Detection in NDVI Time Series: Effects of Inter-Annual Variability and Methodology

Changing trends in ecosystem productivity can be quantified using satellite observations of Normalized Difference Vegetation Index (NDVI). However, the estimation of trends from NDVI time series differs substantially depending on analyzed satellite dataset, the corresponding spatiotemporal resolution, and the applied statistical method. Here we compare the performance of a wide range of trend estimation methods and demonstrate that performance decreases with increasing inter-annual variability in the NDVI time series. Trend slope estimates based on annual aggregated time series or based on a seasonal-trend model show better performances than methods that remove the seasonal cycle of the time series. A breakpoint detection analysis reveals that an overestimation of breakpoints in NDVI trends can result in wrong or even opposite trend estimates. Based on our results, we give practical recommendations for the application of trend methods on long-term NDVI time series. Particularly, we apply and compare different methods on NDVI time series in Alaska, where both greening and browning trends have been previously observed. Here, the multi-method uncertainty of NDVI trends is quantified through the application of the different trend estimation methods. Our results indicate that greening NDVI trends in Alaska are more spatially and temporally prevalent than browning trends. We also show that detected breakpoints in NDVI trends tend to coincide with large fires. Overall, our analyses demonstrate that seasonal trend methods need to be improved against inter-annual variability to quantify changing trends in ecosystem productivity with higher accuracy.

Inter-Annual↗
DOE OSTI2020

Mountain biodiversity and ecosystem functions: interplay between geology and contemporary environments

Although biodiversity and ecosystem functions are strongly shaped by contemporary environments, such as climate and local biotic and abiotic attributes, relatively little is known about how they depend on long-term geological processes. Here, along a 3000-m elevational gradient with tectonic faults on the Tibetan Plateau (that is, Galongla Mountain in Medog County, China), we study the joint effects of geological and contemporary environments on biological communities, such as the diversity and community composition of plants and soil bacteria, and ecosystem functions. We find that these biological communities and ecosystem functions generally show consistent elevational breakpoints at 2000–2800 m, which coincide with Indus-Yalu suture zone fault and are similar to the elevational breakpoints of soil bacteria on another mountain range 1000 km away. Mean annual temperature, soil pH and moisture are the primary contemporary determinants of biodiversity and ecosystem functions, which support previous findings. However, compared with the models excluding geological processes, inclusion of geological effects, such as parent rock and weathering, increases 67.9 and 35.9% of the explained variations in plant and bacterial communities, respectively. Such inclusion increases 27.6% of the explained variations in ecosystem functions. The geological processes thus provide additional links to ecosystem properties, which are prominent but show divergent effects on biodiversity and ecosystem functions: parent rock and weathering exert considerable direct effects on biodiversity, whereas indirectly influence ecosystem functions via interactions with biodiversity and contemporary environments. Thus, the integration of geological processes with environmental gradients could enhance our understanding of biodiversity and, ultimately, ecosystem functioning across different climatic zones.

54 ENVIRONMENTAL SCIENCES↗
NASA NTRS1976

Hardware additions to microprocessor architecture aid software development

An address trap (breakpoint) mechanism and last-in-first-out (LIFO) address stack are suggested as two additions to the basic microprocessor architecture whose functions are solely to aid the programmer. These devices provide the programmer with the ability to specify address breakpoints and to trace program execution back through N instructions, where N is the depth of the stack. Both devices, plus interface logic and buffering, have been designed for an INTEL 8080-based system using approximately 25 integrated-circuit packages.

Sievers, M. W.↗
NASA NTRS1982

Effect of some nitrogen compounds thermal stability of jet A

The effect of known concentrations of some nitrogen containing compounds on the thermal stability of a conventional fuel, namely, Jet A was investigated. The concentration range from 0.01 to 0.1 wt% nitrogen was examined. Solutions were made containing, individually, pyrrole, indole, quinoline, pyridine, and 4 ethylpyridine at 0.01, 0.03, 0.06, and 0.1 wt% nitrogen concentrations in Jet A. The measurements were all made by using a standard ASTM test for evaluating fuel thermal oxidation behavior, namely, ASTM D3241, 'thermal oxidation stability of turbine fuels (JFTOT procedure).' Measurements were made at two temperature settings, and 'breakpoint temperatures' were determined. The results show that the pyrrole and indole solutions have breakpoint temperatures substantially lower than those of the Jet A used.

Antoine, A. C.↗