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Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

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At least 19 records
All sourcesNASA NTRSDOE OSTI
NASA NTRS1983

An investigation of the internal and external aerodynamics of cattle trucks

Wind tunnel tests were conducted on a one-tenth scale model of a conventional tractor trailer livestock hauler to determine the air flow through the trailer and the drag of the vehicle. These tests were conducted with the trailer empty and with a full load of simulated cattle. Additionally, the drag was determined for six configurations, of which details for three are documented herein. These are: (1) conventional livestock trailer empty, (2) conventional trailer with smooth sides (i.e., without ventilation openings), and (3) a stream line tractor with modified livestock trailer (cab streamlining and gap fairing). The internal flow of the streamlined modification with simulated cattle was determined with two different ducting systems: a ram air inlet over the cab and NACA submerged inlets between the cab and trailer. The air flow within the conventional trailer was random and variable. The streamline vehicle with ram air inlet provided a nearly uniform air flow which could be controlled. The streamline vehicle with NACA submerged inlets provided better flow conditions than the conventional livestock trailer but not as uniform or controllable as the ram inlet configuration.

Muirhead, V. U.↗
NASA NTRS2001

Unexprected Changes in Soil Phosphorus Dynamics Following Tropical Deforestation to Cattle Pasture

Phosphorus (P) is widely believed to limit plant growth and organic matter storage in a large fraction of the world's lowland tropical rainforests. We investigated how the most common land use change in such forests, conversion to cattle pasture, affects soil P fractions along forest to pasture chronosequences in the central Brazilian Amazon and in southwestern Costa Rica. Our sites represent a broad range in rainfall, soil type, management strategies, and total soil P (45.2 - 1228.0 microng P / g soil), yet we found some unexpected and at times strikingly similar changes in soil P in all sites. In the Brazilian sites, where rainfall is relatively low and pasture management is more intense than in the Costa Rican sites, significant losses in total soil P and soil organic carbon (SOC) were seen with pasture age on both fine-textured oxisol and highly sandy entisol soils. However, P losses were largely from occluded, inorganic soil P fractions, while organic forms of soil P remained constant or increased with pasture age, despite the declines in SOC. In Costa Rica, SOC remained constant across the oxisol sites and increased from forest to pasture on the mollisols, while total soil P increased with pasture age in both sequences. The increases in total soil P were largely due to changes in organic P; occluded soil P increased only slightly in the mollisols, and remained unchanged in the older oxisols. We suggest that changes in the composition and/or the primary limiting resources of the soil microbial community may drive the changes in organic P. We also present a new conceptual model for changes in soil P following deforestation to cattle pasture.

Townsend, Alan R.↗
DOE OSTI2025

Limited effects of tannin supplementation on the dairy cattle fecal microbiome with modulation of metabolites

Tannins are plant secondary metabolites that bind organic carbon (C) and nitrogen (N), potentially altering substrate bioavailability for enteric fermentation in ruminants. This interaction may reduce greenhouse gas (GHG) emissions and influence nitrogen partitioning. Given tannins' resistance to ruminal degradation and persistence through the gastrointestinal tract, this study investigated the effects of a tannin-based feed additive on fecal microbial diversity, fecal chemical composition, and GHG emissions. Twenty-four early- to mid-lactation dairy cows were randomized to receive either a tannin-based feed additive (TRT; containing condensed and hydrolyzable tannins from Schinopsis quebracho-colorado [Schltdl.]) or a control diet (CON) for 64 days. Cows were blocked by parity, dry matter intake, milk yield, body weight, and days in milk. Fecal samples were collected on days 0, 16, 32, and 64 and analyzed using 16S rRNA gene amplicon sequencing. Fecal C, N, and indole-3-lactate were measured, and GHG emissions (N2O, CH4, CO2) were assessed via 14-day laboratory incubation. A total of 1,538 amplicon sequence variants were identified, with Firmicutes as the dominant phylum. Fecal phylogenetic diversity showed a significant treatment × day interaction (p < 0.01), with TRT cows exhibiting reduced microbial diversity from day 16 to 64. Fecal C and N concentrations were significantly lower (p < 0.01) in TRT cows on day 16, while indole-3-lactate levels were higher on day 64 (p = 0.02). GHG emissions did not differ significantly between treatments. The tannin-based feed additive influenced fecal microbial community structure and select chemical parameters but did not significantly affect GHG emissions from feces. These findings suggest that dietary tannins may modulate gut microbial ecology with minimal impact on downstream manure-related emissions.

Klein, Matthew L↗
NASA NTRS1978

Deforestation planning for cattle grazing in Amazon Basin using LANDSAT data

The author has identified the following significant results. This research did not show the total potential of the LANDSAT system, but tried to open up new research aspects for the utilization of LANDSAT data in natural resource control. Results obtained through this research showed that LANDSAT data can be used to develop monitoring programs in the tropical forest areas of Brazil.

Dejesusparada, N.↗
NASA NTRS1983

Improved Cattle Hauler

Better aerodynamics and ventilation increases fuel efficiency and decreases shipping losses. Trailer is ventilated and cooled by inlet ports in front of rig and outlet ports in middle and rear. Rounded cab and fairing reduce drag by creating an attached airflow.

Saltzman, E. J.↗
DOE OSTI2025

White Rock Canyon Riparian Monitoring

Lands at Los Alamos National Laboratory (LANL) are owned and managed by the Department of Energy, National Nuclear Security Administration (DOE/NNSA). The Laboratory’s eastern boundary is in White Rock Canyon (WRC) along the Rio Grande within Technical Areas (TAs) 70 and 33, with approximately 4 miles of property along the river (Figure 1). Feral cattle have been documented along the Rio Grande in WRC since the 1980s. Cattle activity is known to be especially harmful to riparian ecosystems, which are hubs for biodiversity and crucial habitat used by multiple endangered species in this region, including the Southwestern Willow Flycatcher and Yellow-billed Cuckoo on LANL property (Poessel 2020, Sanchez 2021). To gain a comprehensive understanding of the status of riparian ecosystems within WRC and the damage feral cattle populations are imposing, ecologists at LANL conducted a month-long vegetation monitoring project in September 2025. Riparian vegetation monitoring protocols and rapid assessments of cattle impacts were used to quantify the intensity and extent of cattle damage. The data collected will be used as a baseline for comparison following the removal of feral cattle from the canyon. Abundant cattle sign indicates current and heavy utilization of the riparian areas that were surveyed. There are multiple wallowing areas where soils are completely denuded of vegetation and highly compacted. Based on the severity and extent of impact observed in these areas, cattle use appears consistent and on-going over an extended period of years, supporting the need for removal and restoration management.

54 ENVIRONMENTAL SCIENCES↗
DOE OSTI2024

Morphological and molecular characterization of a Sarcocystis bovifelis-like sarcocyst in American beef

Abstract Background Parasites in the apicomplexan genusSarcocystisinfect cattle worldwide. Assessing the economic importance of each such parasite species requires proper diagnosis.Sarcocystiscruzi,a thin-walled species, infects virtually all cattle. The prevalence of the other thin-walled parasite,Sarcocystisheydorni, remains less well established. The remaining six species all have thick (> 3 µm) cyst walls (Sarcocystishirsuta,S.hominis,S.bovifelis,S.bovini,S.sigmoideus, andS.rommeli). Thick-walled sarcocysts often induce inflammation in striated muscles (causing bovine eosinophilic myositis), leading to condemnation of carcasses at slaughter. One of these,S.hirsuta, can be seen macroscopically and lead to condemnation of beef. TwoSarcocystisspecies,S.hominisandS.heydorni, are zoonotic. AlthoughS.hominishas been reported as prevalent in Europe, the occurrence of thick-walled species in the US remains poorly known. Here, for the first time to our knowldge, we characterize a thick-walledSarcocystisspecies from a sample of beef from a local grocery store in Maryland. By morphological and genetic criteria, it closely, but not perfectly, resembles parasites previously ascribed toS.bovifelis. Methods Beef samples were examined forSarcocystisinfection, using acid-pepsin digestion to search for bradyzoites, microscopically by compression between a glass slide and coverslip, by histology of paraffin embedded sections stained with hematoxylin and eosin, and by transmission electron microscopy (TEM). Molecular characterization was attempted employing genetic markers:18SrRNA,28SrRNA,cox1,ITS1,gapdh1,ron3, andrpoB. Results Molecular evaluation revealed 100% identity withS.bovifelis-like sarcocysts from naturally infected cattle from Germany and Argentina; although the condition of the frozen material precludes complete characterization by TEM, we noted morphological features which differed from theS.bovifelisoriginally described from experimentally infected cattle from Germany. Conclusions A novelSarcocystisspecies is described from beef from the USA but not named until further evaluation. Graphical Abstract

Parasitology↗
DOE OSTI2024

Seroprevalence and risk factors for brucellosis amongst livestock and humans in a multi-herd ranch system in Kagera, Tanzania

Background Brucellosis remains a significant health and economic challenge for livestock and humans globally. Despite its public health implications, the factors driving the endemic persistence ofBrucellaat the human-livestock interface in Tanzania remain poorly elucidated. This study aimed to identify the seroprevalence ofBrucellainfection in livestock and humans within a ranching system and determine associated risk factors for disease endemicity. Methods A cross-sectional sero-epidemiological study was conducted in 2023 in Tanzania’s Karagwe District, involving 725 livestock (cattle, goats, sheep) from 10 herds and 112 humans from associated camps. Seroprevalence was assessed using competitive ELISA while epidemiological data were collected via questionnaires. Generalized Linear Models and Contrast Analysis were used to identify risk factors for infection. Results Overall seroprevalence was 34% in livestock and 41% in humans. Goats exhibited the highest prevalence (69.2%), while cattle had the lowest (22.6%). Mixed-species herds (Odds Ratio, OR = 2.96, CI [1.90–4.60]) and small ruminants-only herds (OR = 6.54, CI [3.65–11.72]) showed a significantly higher risk of seropositivity compared to cattle-only herds. Older cattle (OR = 5.23, CI [2.70–10.10]) and lactating females (OR = 2.87, CI [1.78–4.63]) represented significant risks for brucellosis in livestock. In humans, close contact with animals (OR = 7.20, CI [1.97–36.31]) and handling animals during parturition or aborted fetuses (OR = 2.37, CI [1.01–5.58]) were significant risk factors. Notably, no spatial association was found in seroprevalence between herds and nearby human communities. Conclusion The lack of spatial correlation between livestock and human seroprevalence suggests complex transmission dynamics, potentially involving endemic circulation in livestock and human infections from multiple sources of exposure to livestock. This study highlights the need for comprehensive zoonotic risk education and targeted intervention strategies. Further research is crucial to elucidate transmission pathways and improveBrucellainfection control. This includes developing robust methods for identifying infective species and implementing effective strategies to mitigateBrucellainfection in endemic regions.

Public, Environmental & Occupational Health↗
NASA NTRS1971

A Pilot System for Environmental Monitoring Through Domestic Animals

A pilot system for environmental monitoring is in its early phases of development in Northern California. It is based upon the existing nation wide Federal-State Market Cattle Testing (14CT) program for brucellosis in cattle. This latter program depends upon the collection of blood program at the time of identified cattle. As the cattle Population of California is broadly distributed throughout the state, we intend to utilize these blood samples to biologically monitor the distribution and intensity of selected environmental pollutants. In a 2-year preliminary trial, the feasibility of retrieving, utilizing for a purpose similar to this, and tracing back to their geographic areas of origin of MCT samples have been demonstrated.

Schwabe, Calvin W.↗
NASA NTRS2022

Colorado Eastern Plains Agriculture: Rangeland Monitoring to Inform Grazing Management in Eastern Colorado

Adaptive management on cattle ranches requires rangeland managers to decide the location and duration of the cattle grazing activity across different pastures. Biodiversity, forage availability, and cattle health are all affected by rangeland management. Virtual fencing is a tool that rangeland managers can use to potentially increase biodiversity and improve ranching operations. NASA DEVELOP and Colorado State University (CSU) collaborated with the Nature Conservancy (TNC), and Red Top Ranch to demonstrate the efficacy of virtual fencing. We sought to identify annual and monthly biomass patterns on the ranch through the creation of monthly max biomass productivity maps. We utilized a dataset from the Agricultural Research Service (ARS) to calculate biomass on the ranch. To validate our remotely-sensed results, we compared model-predicted biomass values to field-collected biomass clipping data and an additional biomass dataset from the Rangeland Analysis Platform (RAP). We used satellite imagery from Landsat 5 Thematic Mapper (TM), Landsat 7 Enhanced Thematic Mapper Plus (ETM+), Landsat 8 Operational Land Imager (OLI), Landsat 9 –OLI-2, and Sentinel-2 MultiSpectral Instrument (MSI) for 2021 and 2022. We found that there was heterogeneity in biomass across the ranch, with higher biomass on the western side. The highest peak of biomass was in the summer months, with a smaller peak in mid-September. The ARS biomass dataset had a significant relationship with RAP for 2021. ARS biomass did not have a significant relationship with the biomass field data collected in 2022. The results of our study are aimed to support rotation management, ranch production, biodiversity, and conservation management.

cattle↗
DOE OSTI2025

National serosurvey and risk mapping reveal widespread distribution of Coxiella burnetii in Kenya

Coxiella burnetii, the causative agent of Q fever, is an emerging pathogen that has the potential to cause severe chronic infections in animals and humans worldwide. The detrimental impact on public health is projected to be higher in the low- and middle-income countries given their lower capacity to sustain effective surveillance and response measures. We implemented a national serosurvey of cattle in Kenya to map the spatial distribution of the pathogen. The study used serum samples that were collected from randomly selected cattle in different ago-ecological zones across the country. These samples were screened for the pathogen using PrioCHECK Ruminant Q Fever AB Plate ELISA kit. The laboratory findings were analyzed using INLA package to identify risk factors for C. burnetii exposure from herd- and animal-level factors, area, and bioclimatic datasets accessed from online databases. A total of 6,593 cattle were recruited for the study; of these, 7.9% (95% CI; 7.2–8.5) were seropositive. Outputs from the multivariable analysis revealed that the animal age and some of the geographical variables including wind speed, area under shrubs and “petric calcisols” type of soil were significantly associated with C. burnetii seropositivity. Being a calf, weaner or subadult was associated with lower odds of exposure compared to being an adult by 0.24 (credibility interval: 2.5% and 97.5%), 0.41 (0.30–0.55) and 0.51 (0.38–0.69), respectively. In addition, a unit increase in the wind speed increased the odds of C. burnetii seropositivity by 1.27 (1.05–1.52) while an increase on the land area under shrubs was associated with lower odds of exposure (0.67 [0.47–0.69]). The effect of petric calcisols was non-linear; an increase of the land area with this soil type was associated with an exponential increase in C. burnetii seropositivity. This study provides new data on C. burnetii seroprevalence, information of its risk factors and a prevalence map that can be used for C. burnetii risk surveillance and control. The identification of environmental risk factors for C. burnetii exposure, and the increasing awareness of the zoonotic potential of the pathogen, calls for the need to enhance the existing collaborations for the surveillance and control of C. burnetii in line with the One Health framework. The evidence generated on the potential role of environmental factors can also be used to design nature-based interventions, such as replacement of vegetation in denuded areas, to reduce potential for the aerosolization of the pathogen. Livestock vaccination in the hotspots would also reduce animal infections and hence the contamination of the environment.

60 APPLIED LIFE SCIENCES↗
DOE OSTI2024

Activity of Cannabidiol on Ex Vivo Amino Acid Fermentation by Bovine Rumen Microbiota

Amino-acid-fermenting bacteria are wasteful organisms within the rumens of beef cattle that remove dietary amino nitrogen by producing ammonia, which is then excreted renally. There are currently no on-label uses for the control of this microbial guild, but off-label use of broad-spectrum antimicrobials has shown efficacy, which contributes to antimicrobial resistance. Plant-derived antimicrobials supplemented into the diets of cattle may offer worthwhile alternatives. This study sought to investigate the role of cannabidiol (CBD) as a terpenophenolic antimicrobial. Ex vivo cell suspensions were harvested from the rumen fluid of Angus × Holstein steers in non-selective media with amino acid substrates. The suspensions were treated with five concentrations of CBD (860 μg mL−1–0.086 μg mL−1) and incubated (24 h), after which ammonia production and viable number of cells per substrate and treatment were measured. The data demonstrated a ~10–15 mM reduction in ammonia produced at the highest concentration of CBD and negligible changes in the viable number of amino-acid-fermenting bacteria. CBD does not appear to be a biologically or economically viable terpenophenolic candidate for the control of amino acid fermentation in beef cattle.

Biotechnology & Applied Microbiology↗
DOE OSTI2025

Longitudinal seroprevalence of Crimean-Congo hemorrhagic fever virus in Southern Uganda

Crimean-Congo hemorrhagic fever (CCHF) is a tick-borne disease endemic to many regions of Africa, the Middle East, Southeast Asia and the Balkans. Caused by the CCHF virus (CCHFV), CCHF has been a recognized cause of illness in Uganda since the 1950s and recently, more intensive surveillance suggests CCHFV is widely endemic within the country. Most surveillance has been focused on the Ugandan cattle corridor due to the risk of CCHFV exposure associated with livestock practices. Here we evaluated the seroprevalence of CCHFV in several Southern Ugandan communities outside the cattle corridor combined with longitudinal sample sets to measure the immune response to CCHFV for up to a decade. Interestingly, across three community types, agrarian, trading and fishing, we detected CCHFV seroprevalence in all three but found the highest seroprevalence in fishing communities. We also measured consistent CCHFV-specific antibody responses for up to a decade. Our findings support the conclusion that CCHFV is widely endemic in Uganda and highlight that additional communities may be at risk for CCHFV exposure.

60 APPLIED LIFE SCIENCES↗
DOE OSTI2024

CRISPR-prime editing, a versatile genetic tool to create specific mutations with a single nucleotide resolution in Leptospira

ABSTRACT Leptospirosis, caused by pathogenic bacteria from the genusLeptospira, is a global zoonosis responsible for more than one million human cases and 60,000 deaths annually. The disease also affects many domestic animal species. Historically, genetic manipulation ofLeptospirahas been difficult to perform, resulting in limited knowledge on pathogenic mechanisms of disease and the identification of virulence factors. The application of CRISPR/Cas9 and its variations have helped fill these gaps but the generation of knockout mutants remains challenging because double-strand breaks (DSBs) inflicted by Cas9 nuclease are lethal toLeptospiracells. The novel CRISPR prime editing (PE) strategy is the first precise genome-editing technology that allows deletions, insertions, and base substitutions without introducing DSBs. This revolutionary technique utilizes a nickase Cas9 that cleaves a single strand of DNA, coupled with an engineered reverse transcriptase and a modified single-guide RNA (termed prime editing guide RNA) containing an extended 3′ end with the desired edits. We demonstrate the application of CRISPR-PE in both saprophytic and pathogenicLeptospirafrom multiple species and serovars by introducing deletions or insertions into target DNA with a remarkable precision of just one nucleotide. Additionally, we demonstrate the ability to genetically manipulateLeptospira borgpetersenii, a prevalent pathogenic species of humans, domestic cattle, and wildlife animals. Rapid plasmid loss by mutated strains in liquid culture allows for the generation of knockout strains without selective markers, which can be readily used to elucidate virulence factors and develop optimized bacterin and/or live vaccines against leptospirosis. IMPORTANCE Leptospirosis is a geographically widespread bacterial zoonosis. Genetic manipulation of pathogenicLeptospiraspp. has been laborious and difficult to perform, limiting our ability to understand how leptospires cause disease. The application of the CRISPR/Cas9 system toLeptospiraenhanced our ability to generate knockdown and knockout mutants; however, the latter remains challenging. Here, we demonstrate the application of the CRISPR prime editing technique inLeptospira, allowing the generation of knockout mutants in several pathogenic species, with mutations comprising just a single nucleotide resolution. Notably, we generated a mutant in theLeptospira borgpeterseniibackground, a prevalent pathogenic species of humans and cattle. Our application of this method opens new avenues for studying pathogenic mechanisms ofLeptospiraand the identification of virulence factors across multiple species. These methods can also be used to facilitate the generation of marker-less knockout strains for updated and improved bacterin and/or live vaccines.

Microbiology↗
DOE OSTI2026

AmeriFlux US-NC5 NC Butner Farm

This is the AmeriFlux version of the carbon flux data for the site US-NC5 NC Butner Farm. Site Description - The US-NC5 flux tower is located within an 80-year-old mixed pine-hardwood forest at the Umstead Research Farm in Butner, North Carolina. The northern section of this 20-hectare Fall Lake Watershed of the Neuse River Basin in the Piedmont of North Carolina, USA. The Northern portion is currently a managed cattle farm, which is slated for expansion—necessitating forest clearing in the flux site. To establish a reference baseline, a year-long, all-season eddy covariance flux monitoring campaign will be conducted from April 2025 to March 2026. This effort aims to capture the carbon flux dynamics of the mature forest ecosystem prior to a planned land-use conversion. The site will be transitioned into a silvopasture, maintained through prescribed burning and cattle grazing to promote an open-canopy watershed structure. Flux measurements will continue after the conversion.

Sun, Ge [USDA Forest Service]↗