Rapid and inexpensive micro-electroelution of nucleic acid and protein from agarose and polyacrylamide gels
No abstract available
NASA Discipline Plant Biology↗
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Results for “DNA/isolation & purification”
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Separation of mouse testis cells on a Celsep (TM) apparatus and their usefulness as a source of high molecular weight DNA or RNA
The use of a self-contained unit-gravity cell separation apparatus for separation of populations of mouse testicular cells is described. The apparatus, a Celsep (TM), maximizes the unit area over which sedimentation occurs, reduces the amount of separation medium employed, and is quite reproducible. Cells thus isolated have been good sources for isolation of DNA, and notably, high molecular weight RNA.
NASA Discipline Cell Biology↗
Purification of plasmid DNA using selective precipitation by compaction agents
A scaleable method for the liquid-phase separation of plasmid DNA from RNA.
Non-NASA Center↗
Enhancement of anion-exchange chromatography of DNA using compaction agents
The use of adsorptive chromatography for preparative nucleic acid separations is often limited by low capacity. The possibility that the adsorbent surface area sterically accessible to nucleic acid molecules could be increased by reducing their radius of gyration with compaction agents has been investigated. The equilibrium adsorption capacity of Q Sepharose anion-exchange matrix for plasmid DNA at 600 mM NaCl was enhanced by up to ca. 40% in the presence of 2.5 mM spermine. In addition, compaction agent selectivity has been demonstrated. Spermine, for example, enhances the adsorption of both plasmid and genomic DNA, spermidine enhances binding only of plasmid, and hexamine cobalt enhances only the binding of genomic DNA. Compaction may be generally useful for enhancing adsorptive separations of nucleic acids.
NASA Discipline Environmental Health↗