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Results for “De novo design”

Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

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4 records
All sourcesNASA NTRSDOE OSTI
NASA NTRS2019

De novo design of symmetric ferredoxins that shuttle electrons in vivo

A symmetric origin for bacterial ferredoxins was first proposed over 50 y ago, yet, to date, no functional symmetric molecule has been constructed. It is hypothesized that extant proteins have drifted from their symmetric roots via gene duplication followed by mutations. Phylogenetic analyses of extant ferredoxins support the independent evolution of N- and C-terminal sequences, thereby allowing consensus-based design of symmetric 4Fe-4S molecules. All designs bind two [4Fe-4S] clusters and exhibit strongly reducing midpoint potentials ranging from −405 to −515 mV. One of these constructs efficiently shuttles electrons through a designed metabolic pathway in Escherichia coli. These finding establish that ferredoxins consisting of a symmetric core can be used as a platform to design novel electron transfer carriers for in vivo applications. Outer-shell asymmetry increases sequence space without compromising electron transfer functionality.

Andrew C. Mutter↗
NASA NTRS2007

Investment and Return in International Space Life Sciences Research Cooperation

Today, a worldwide community of life scientists interested in space research is attempting to improve the understanding of general biological processes, aid the development of procedures to reduce the biomedically-related risks of space flight, and/or directly support the health care of people who fly in space. Unfortunately, limited resource and subject availability and the technical challenges of performing space experiments have all hampered the full growth and development of space life sciences research. For many years, international cooperation in this field has been considered an attractive approach towards overcoming some of these difficulties, since pooling resources and sharing results would enhance the knowledge of all cooperating partners. International cooperative activities, however, require an investment by each partner and, just as in many other endeavors, the research gain can be directly related to the investment made. In this paper, the authors will discuss three possible levels of cooperation: sharing of data from independent investigations, harmonious integration of pre-designed independent investigations, and de novo design of an integrated suite of investigations using a joint investigator team. The degree of investment and potential return for each level of cooperation will be described.

McPhee, Jancy C.↗
NASA NTRS2017

A Quantitative Risk-Benefit Analysis of Prophylactic Surgery Prior to Extended-Duration Spaceflight

INTRODUCTION: Among otherwise healthy astronauts undertaking deep space missions, the risks for acute appendicitis (AA) and cholecystitis (AC) are not zero. If these conditions were to occur during spaceflight they may require surgery for definitive care. The proposed study quantifies and compares the risks of developing de novo AA and AC in-flight to the surgical risks of prophylactic laparoscopic appendectomy (LA) and cholecystectomy (LC) using NASA's Integrated Medical Model (IMM). METHODS: The IMM is a Monte Carlo simulation that forecasts medical events during spaceflight missions and estimates the impact of these medical events on crew health. In this study, four Design Reference Missions (DRMs) were created to assess the probability of an astronaut developing in-flight small-bowel obstruction (SBO) following prophylactic 1) LA, 2) LC, 3) LA and LC, or 4) neither surgery (SR# S-20160407-351). Model inputs were drawn from a large, population-based 2011 Swedish study that examined the incidence and risks of post-operative SBO over a 5-year follow-up period. The study group included 1,152 patients who underwent LA, and 16,371 who underwent LC. RESULTS: Preliminary results indicate that prophylactic LA may yield higher mission risks than the control DRM. Complete analyses are pending and will be subsequently available. DISCUSSION: The risk versus benefits of prophylactic surgery in astronauts to decrease the probability of acute surgical events during spaceflight has only been qualitatively examined in prior studies. Within the assumptions and limitations of the IMM, this work provides the first quantitative guidance that has previously been lacking to this important question for future deep space exploration missions.

Carroll, Danielle↗
NASA NTRSDate not supplied

The Coronal Microscale Observatory

It has been a longstanding challenge to identify the mechanisms responsible for heating the solar corona, in part because heating, whether by waves or magnetic reconnection, is thought to be concentrated in thus far unresolved volumes with characteristic scales ≲100 km. The Coronal Microscale Observatory (CMO) is a mission concept designed to image these microscale heating events, identify the dominant physical mechanisms that control their initiation and evolution, and understand their effects on the formation of the solar wind. CMO positions three spacecraft and three instruments near the Sun-Earth L1 Lagrange point. One instrument is a cluster of 6 coaligned extreme ultraviolet (EUV) telescopes that image a common field of view with ultrahigh angular resolution (0.02−0.07 arcsec) in narrow wavelength bands, each sensitive to emission from plasma in a limited temperature range. The second instrument is a multi-band, full-disk, externally occulted coronagraph. Finally, a two-band fine scale EUV imager (resolution 0.3 arcsec) provides a larger field of view for context and additional science. The three CMO craft fly in precise formation to ensure that the EUV imagers point to a desired target on the Sun and the external occulter accurately blocks the solar disk. The novel mission architecture arises from the intrinsically long EUV focal length (≳100 m) of diffractive optics known as photon sieves, which achieve nearly diffraction-limited EUV imaging but require a distributed telescope, in which the optics and- the image sensors are on separate spacecraft. Two spacecraft are also needed to position an external occulter 200 m in front of the coronagraph, which enables visible-light imaging of the corona very close to the solar limb with undiminished angular resolution. Recent advances in fabricating ultraprecise and smooth reflective optics suggest that a conventional (single spacecraft)EUV “microscope” may now be feasible in an Explorer-class mission that could achieve a subset of the scientific objectives of CMO.

Douglas Rabin↗