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Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

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At least 19 records
All sourcesNASA NTRSDOE OSTI
NASA NTRS1999

Detection of endogenous alkaline phosphatase activity in intact cells by flow cytometry using the fluorogenic ELF-97 phosphatase substrate

BACKGROUND: The alkaline phosphatase (AP) substrate 2-(5'-chloro-2'-phosphoryloxyphenyl)-6-chloro-4-(3H)-quinazolinone (ELF((R))-97 for enzyme-labeled fluorescence) has been found useful for the histochemical detection of endogenous AP activity and AP-tagged proteins and oligonucleotide probes. In this study, we evaluated its effectiveness at detecting endogenous AP activity by flow cytometry. METHODS: The ELF-97 phosphatase substrate was used to detect endogenous AP activity in UMR-106 rat osteosarcoma cells and primary cultures of chick chondrocytes. Cells were labeled with the ELF-97 reagent and analyzed by flow cytometry using an argon ultraviolet (UV) laser. For comparison purposes, cells were also assayed for AP using a Fast Red Violet LB azo dye assay previously described for use in detecting AP activity by flow cytometry. RESULTS: The ELF-97 phosphatase substrate effectively detected endogenous AP activity in UMR-106 cells, with over 95% of the resulting fluorescent signal resulting from AP-specific activity (as determined by levamisole inhibition of AP activity). In contrast, less than 70% of the fluorescent signal from the Fast Red Violet LB (FRV) assay was AP-dependent, reflecting the high intrinsic fluorescence of the unreacted components. The ELF-97 phosphatase assay was also able to detect very low AP activity in chick chondrocytes that was undetectable by the azo dye method. CONCLUSIONS: The ELF-97 phosphatase assay was able to detect endogenous AP activity in fixed mammalian and avian cells by flow cytometry with superior sensitivity to previously described assays. This work also shows the applicability of ELF-97 to flow cytometry, supplementing its previously demonstrated histochemical applications. Copyright 1999 Wiley-Liss, Inc.

Non-NASA Center↗
NASA NTRS1972

ELF noise bands associated with auroral electron precipitation.

Observation of a new type of ELF noise band that is closely associated with low-energy auroral electron precipitation. These observations have been made at relatively low altitudes (less than 3000 km) with the polar-orbiting satellite Injun 5. The noise bands typically have a center frequency of 100 to 300 Hz and often appear to consist of many nearly monochromatic bursts, typically of a few seconds' duration, superimposed to produce the observed noise band. These ELF noise bands are observed only in a relatively narrow range of latitudes (a few degrees) in the auroral zone and are almost always associated with intense fluxes of precipitating electrons with energies from a few hundred electron volts to several kiloelectron volts. On the dayside of the magnetosphere the region where the ELF noise bands and the associated low-energy electron precipitation are observed has been identified as the polar cusp. In considering the possible explanations of these ELF noise bands, it is noted that the spectral characteristics of this noise are very similar to a type of narrowband electromagnetic noise called 'lion's roar,' which has been observed at much higher altitudes in the magnetosheath with the satellite Ogo 5. It is suggested that the ELF noise bands observed at low altitudes with Injun 5 are caused by lion's roar emissions that have propagated down 'open' magnetic-field lines to low altitudes from the magnetosheath region.

Gurnett, D. A.↗
NASA NTRS2001

Numerical Simulation of an Enclosed Laminar Jet Diffusion Flame in Microgravity Environment: Comparison with ELF Data

Enclosed diffusion flames are commonly found in practical combustion systems, such as the power-plant combustor, gas turbine combustor, and jet engine after-burner. In these systems, fuel is injected into a duct with a co-flowing or cross-flowing air stream. The diffusion flame is found at the surface where the fuel jet and oxygen meet, react, and consume each other. In combustors, this flame is anchored at the burner (i.e., fuel jet inlet) unless adverse conditions cause the flame to lift off or blow out. Investigations of burner stability study the lift off, reattachment, and blow out of the flame. Flame stability is strongly dependent on the fuel jet velocity. When the fuel jet velocity is sufficiently low, the diffusion flame anchors at the burner rim. When the fuel jet velocity is increased, the flame base gradually moves downstream. However, when the fuel jet velocity increases beyond a critical value, the flame base abruptly jumps downstream. When this "jump" occurs, the flame is said to have reached its lift-off condition and the critical fuel jet velocity is called the lift-off velocity. While lifted, the flame is not attached to the burner and it appears to float in mid-air. Flow conditions are such that the flame cannot be maintained at the burner rim despite the presence of both fuel and oxygen. When the fuel jet velocity is further increased, the flame will eventually extinguish at its blowout condition. In contrast, if the fuel jet velocity of a lifted flame is reduced, the flame base moves upstream and abruptly returns to anchor at the burner rim. The fuel jet velocity at reattachment can be much lower than that at lift off, illustrating the hysteresis effect present in flame stability. Although there have been numerous studies of flame stability, the controlling mechanisms are not well understood. This uncertainty is described by Pitts in his review of various competing theories of lift off and blow out in turbulent jet diffusion flames. There has been some research on the stability of laminar flames, but most studies have focused on turbulent flames. It is also well known that the airflow around the fuel jet can significantly alter the lift off, reattachment and blow out of the jet diffusion flame. Buoyant convection is sufficiently strong in 1-g flames that it can dominate the flow-field, even at the burner rim. In normal-gravity testing, it is very difficult to delineate the effects of the forced airflow from those of the buoyancy-induced flow. Comparison of normal-gravity and microgravity flames provides clear indication of the influence of forced and buoyant flows on the flame stability. The overall goal of the Enclosed Laminar Flames (ELF) investigation (STS-87/USMP-4 Space Shuttle mission, November to December 1997) is to improve our understanding of the effects of buoyant convection on the structure and stability of co-flow diffusion flame, e.g., see http://zeta.lerc.nasa.gov/expr/elf.htm. The ELF hardware meets the experiment hardware limit of the 35-liter interior volume of the glovebox working area, and the 180x220-mm dimensions of the main door. The ELF experiment module is a miniature, fan-driven wind tunnel, equipped with a gas supply system. A 1.5-mm diameter nozzle is located on the duct's flow axis. The cross section of the duct is nominally a 76-mm square with rounded corners. The forced air velocity can be varied from about 0.2 to 0.9 m/s. The fuel flow can be set as high as 3 std. cubic centimeter (cc) per second, which corresponds to a nozzle exit velocity of up to 1.70 m/s. The ELF hardware and experimental procedure are discussed in detail in Brooker et al. The 1-g test results are repeated in several experiments following the STS-87 Mission. The ELF study is also relevant to practical systems because the momentum-dominated behavior of turbulent flames can be achieved in laminar flames in microgravity. The specific objectives of this paper are to evaluate the use reduced model for simulation of flame lift-off and blowout.

Jia, Kezhong↗
NASA NTRS1987

Extremely Luminous Far-infrared Sources (ELFS)

The Infrared Astronomical Satellite (IRAS) survey uncovered a class of Extremely Luminous Far Infrared Sources (ELFS), exhibiting luminosities up to and occasionally exceeding 10 to the 12th power L sub 0. Arguments are presented to show that sources with luminosities L equal to or greater than 3 x 10 to the 10th power L sub 0 may represent gas rich galaxies in collision. The more conventional explanation of these sources as sites of extremely active star formation fails to explain the observed low optical luminosities of ELFS as well as their high infrared excess. In contrast, a collisional model heats gas to a temperature of approx. 10 to the 6th power K where cooling takes place in the extreme ultraviolet. The UV is absorbed by dust and converted into far infrared radiation (FIR) without generation of appreciable optical luminosity. Gas recombination as it cools generates a Lyman alpha photon only once for every two extreme ultraviolet approx. 50eV photons emitted by the 10 to the 6th power gas. That accounts for the high infrared excess. Finally, the model also is able to explain the observed luminosity distribution of ELFS as well as many other traits.

Harwit, Martin↗
NASA NTRS1988

ELF electric turbulence near an electron beam-emitting rocket in the auroral ionosphere - Echo 6

Orthogonal double-probes on the Echo 6 free-lying plasma diagnostic payload were used to study ELF turbulent electric fields during 36-keV beam injections. The observed characteristics of the ELF fields can best be explained by the current systems set up around the entire system rather than by a direct beam-plasma interaction. Frequency-power spectra were calculated for two different polarizations as a function of the probe-gun payload distance, and measurements were obtained of the lower hybrid waves near 8.5 kHz and whistler mode waves near 125 kHz. The dispersion of a current instability was calculated in order to investigate the generation mechanism of the observed ELF electric turbulence.

Abe, Y.↗
NASA NTRS1974

Intensity variation of ELF hiss and chorus during isolated substorms

Electromagnetic ELF emissions (100-1000 Hz) observed on the polar-orbiting OGO-6 satellite within three hours of the dawn-dusk meridian consistently exhibit a predictable response to isolated substorm activity. Near dawn, the emissions intensify during the substorm and then subside following the magnetic activity; the waves are most intense at L greater than 4, exhibit considerable structure and have been primarily identified as chorus. At dusk the response is entirely different; the wave intensity falls to background levels during substorm activity but subsequently intensifies, usually reaching levels well in excess of that before the disturbance. The emissions near dusk extend to low L, are relatively featureless, and have been identified as plasmaspheric hiss. These features are interpreted in terms of changes in the drift orbits of outer-zone electrons which cyclotron resonate with ELF waves.

Thorne, R. M.↗
NASA NTRS1977

The local time variation of ELF emissions during periods of substorm activity

A statistical study is reported concerning the occurrence probability of ELF emissions observed on the low-altitude polar-orbiting satellite OGO 6 during periods of substorm activity. Over 160 individual substorm periods have been selected and analyzed during the period from June 1969 to October 1970. The statistical results are discussed, taking into account ELF response to substorm activity and emissions during the recovery phase of magnetic storms.

Thorne, R. M.↗
NASA NTRS1979

Field-aligned currents, convection electric fields, and ULF-ELF waves in the cusp

Nearly simultaneous observations from the Triad and Hawkeye satellites over the Southern Hemisphere, at low altitudes near the noon meridian and close to the usual polar cusp latitudes, show that in and near the polar cusp there exist several relationships between field-aligned currents (FACs), convection electric fields, ULF-ELF magnetic noise, broadband electrostatic noise and interplanetary magnetic fields. The most important findings are (1) the FACs directed into the ionosphere in the noon-to-dusk local time sector and directed away from the ionosphere in the noon-to-dawn local time sector and identified as region-1 permanent FACs (Iijima and Potemra, 1976a) and are located equatorward of the regions of antisunward (westward) convection; (2) the observations are consistent with a two-cell convection pattern symmetric in one case (throat positioned at noon) and asymmetric in another (throat located in a sector on the forenoon side in juxtaposition to the region of strong convection on the afternoon side); and (3) fine-structure FACs are responsible for the generation of ULF-ELF noise in the polar cusp.

Saflekos, N. A.↗
NASA NTRS1985

ELF wave production by an electron beam emitting rocket system and its suppression on auroral field lines - Evidence for Alfven and drift waves

Orthogonal probes on a free-flying plasma diagnostics payload are used to study ELF electric disturbances in the auroral ionosphere that are due to the injection of powerful electron beams. Frequency spectrograms are presented for various pitch angles, pulsing characteristics, and other properties of the injected beams; the large scale DC ionospheric convection electric field is measured, together with auroral particle precipitation, visual auroral forms, and ionospheric parameters. In view of the experimental results obtained, it is postulated that the observed ELF waves are in the Alfven and drift modes, and are generated by the positive vehicle potential during beam injection.

Winckler, J. R.↗
NASA NTRS1986

ELF waves and ion resonances produced by an electron beam emitting rocket in the ionosphere

Results are reported from the ECHO-6 electron-beam-injection experiment, performed in the auroral-zone ionosphere on March 30, 1983 using a sounding rocket equipped with two electron guns and a free-flying plasma-diagnostics instrument package. The data are presented in extensive graphs and diagrams and characterized in detail. Large ELF wave variations, superposed on the strong beam-sector-directed quasi-dc component, are observed in the 100-eV beam-induced plasma when the beam is injected in a transverse spiral, but not when it is injected upward parallel to the magnetic-field line. ELF activity is found to be suppressed whenever the rocket passed through field lines with auroral activity, suggesting that the waves are produced by the interaction of the beam potentials, plasma currents, and return currents neutralizing the accelerator payload.

Winckler, J. R.↗
NASA NTRS1987

ELF oscillations associated with electron beam injections from the Space Shuttle

ELF oscillations (f less than 500 Hz) were observed during the electron beam emissions of the space experiments with particle accelerators (SEPAC) flown on the Spacelab 1 Shuttle mission. The beams had energies up to 5 keV and currents up to 300 mA, and the oscillations were present in the data from a Langmuir probe, a floating probe, an electron energy analyzer, and a photometer. The power density and frequency of the fundamental increased with the Shuttle charge-up potential. The emission level observed during the beam sequences increased with the charge-up potential of the Orbiter, which largely depended on the wake structure. It is most likely that the ELF oscillations are expressions of fluctuations in the return current and the Shuttle potential and that these fluctuations are caused by processes involving charge imbalances in the near environment of the Shuttle, possibly in a comoving plasma cloud.

Cai, D.↗
NASA NTRS2003

Investigations of Relatively Easy To Construct Antennas With Efficiency in Receiving Schumann Resonances: Preparations for a Miniaturized Reconfigurable ELF Receiver

Relatively little is known about the cavity between the Earth and the ionosphere, which opens opportunities for technological advances and unique ideas. One effective means to study this cavity is with extremely low frequency (ELF) antennas. Possible applications of these antennas are global weather prediction, earthquake prediction, planetary exploration, communication, wireless transmission of power, or even a free energy source. The superconducting quantum interference device SQUID) and the coil antenna are the two most acceptable receivers discovered for picking up ELF magnetic fields. Both antennas have the potential for size reduction, allowing them to be portable enough for access to space and even for personal ware. With improvements of these antennas and signal processing, insightful analysis of Schumann resonance (SR) can give the science community a band of radio frequency (RF) signals for improving life here on Earth and exploring beyond.

Farmer, Brian W.↗