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High LET Radiation Can Enhance TGF(Beta) Induced EMT and Cross-Talk with ATM Pathways

The TGF(Beta) pathway has been shown to regulate or directly interact with the ATM pathway in the response to radiation in mammary epithelial cells. We investigated possible interactions between the TGF(Beta) and ATM pathways following simulated space radiation using hTERT immortalized human esophageal epithelial cells (EPC-hTERT), mink lung epithelial cells (Mv1lu), and several human fibroblast cell lines. TGF(Beta) is a key modulator of the Epithelial-Mesenchymal Transition (EMT), important in cancer progression and metastasis. The implication of EMT by radiation also has several lines of developing evidence, however is poorly understood. The identification of TGF(Beta) induced EMT can be shown in changes to morphology, related gene over expression or down regulation, which can be detected by RT-PCR, and immunostaining and western blotting. In this study, we have observed morphologic and molecular alternations consistent with EMT after Mv1lu cells were treated with TGF(Beta) High LET radiation enhanced TGF(Beta) mediated EMT with a dose as low as 0.1Gy. In order to consider the TGF(Beta) interaction with ATM we used a potent ATM inhibitor Ku55933 and investigated gene expression changes and Smad signaling kinetics. Ku559933 was observed to reverse TGF(Beta) induced EMT, while this was not observed in dual treated cells (radiation+TGF(Beta)). In EPC-hTERT cells, TGF(Beta) alone was not able to induce EMT after 3 days of application. A combined treatment with high LET, however, significantly caused the alteration of EMT markers. To study the function of p53 in the process of EMT, we knocked down P53 through RNA interference. Morphology changes associated with EMT were observed in epithelial cells with silenced p53. Our study indicates: high LET radiation can enhance TGF(Beta) induced EMT; while ATM is triggering the process of TGF(Beta)-induced EMT, p53 might be an essential repressor for EMT phenotypes.

Wang, Minli

Landsat-7 EMT+ On-Orbit Radiometric Calibration

Landsat-7 was launched on April 15, 1999 and completed its on orbit initialization and verification period on June 28, 1999. The ETM+ payload is similar to the TM sensors on previous Landsat satellites and incorporates two new devices to improve its absolute radiometric calibration. The Full Aperture Solar Calibrator (FASC) is a deployable diffuser panel. This device has been deployed 9 times to date, with a normal deployment schedule of once per month. The initial analysis of the FASC data has given absolute calibration results within 5% of the prelaunch integrating sphere calibrations and a range of variation of 2% between dates. The Partial Aperture Solar Calibrator (PASC), is a set of auxiliary optics that allows the ETM+ to view the sun through a reduced aperture. Data have normally been acquired on a daily basis with the PASC. Initial results with the PASC were encouraging, despite some unexpected saturation in the shortest wavelength band. The response of the ETM+ short wavelength (silicon) bands to the PASC increased initially and has begun to decrease in some of these bands. The longer wavelength (InSb) bands have shown up to 30% oscillations that vary between detectors within the band. Studies are ongoing to better characterize the response to the PASC. The ETM+ also incorporates an internal calibrator (IC), a shutter that oscillates in front of the focal plane that directs light from the internal calibrator lamps to the focal plane. The responses to this device are also varying, though differently than the PASC results. Both the IC and PASC results are attributable to the calibration devices as opposed to the ETM+ itself.

Markham, Brian L.

EMT Project History and Overview

This is a slide set that is part of the 2021 ETM Workshop's planned technical presentations. The content of the presentation is intended to first provide an introduction to the impetus and background that led to ETM and the identified challenges that needed to be considered. Following the introduction, a presentation of early engagement with Industry and other government agencies is provided. The presentation concludes with the early work that followed engagement and the eventual project that ETM was integrated with as well as the eventual formulation of a joint NASA-FAA Research Transition Team.

Upper E Traffic Management

High-power and 2.5 kW advanced-technology ion thruster

Investigations for improving ion thruster components in the 30 cm engineering model thruster (EMT) resulted in the demonstration of useful techniques for grid short removal and discharge chamber erosion monitoring, establishment of relationships between double ion production and thruster operating parameters, verification of satisfactory specifications on porous tungsten vaporizer material and barium impregnated porous tungsten inserts, demonstration of a new hollow cathode configuration, and specification of magnetic circuit requirements for reproducing desired magnetic mappings. The capacity of a 30 cm EMT to operate at higher beam voltages and currents (higher power) was determined. Operation at 2 A beam current and higher beam voltage is shown to be essentially equivalent to operation at 1.1 kV with regard to efficiency, lifetime and operating conditions. The only additional requirement is an improvement in high voltage insulation and propellant isolator capacity. Operation at minimum voltage and higher beam currents is shown to increase thruster discharge chamber erosion in proportion to beam current. Studies to find alternatives to molybdenum for manufacturing ion optics grids are also reported.

Poeschel, R. L.

The 5200 cycle test of an 8-cm diameter Hg ion thruster

An accelerated cycle test was conducted in which an 8-cm Engineering Model Thruster (EMT) prototype successfully completed 5200 on-off cycles and a total of more than 1300hours of thruster operation at a 4.5 mN thrust level. Cathode tip heater powers required for starting and keeper voltages remained well within acceptable limits. The discharge chamber utilization and electrical efficiency were nearly constant over the duration of the test. It was concluded that on-off cyclic operation by itself does not appreciably degrade starting capability or performance of the 8-cm EMT.

Mantenieks, M. A.

5200 cycle of an 8-cm diameter Hg ion thruster

An accelerated cycle test was conducted in which an 8-cm Engineering Model Thruster (EMT) prototype successfully completed 5200 on-off cycles and a total of more than 1300 hours of thruster operation at a 4.5 mN thrust level. Cathode tip heater powers required for starting and keeper voltages remained well within acceptable limits. The discharge chamber utilization and electrical efficiency were nearly constant over the duration of the test. It is concluded that on-off cyclic operation by itself does not appreciably degrade starting capability or performance of the 8-cm EMT.

Mantenieks, M. A.

8-cm Engineering Model Thruster technology - A review of recent developments

Recent testing of the NASA Lewis Research Center/Hughes 8-cm Engineering Model Thruster (EMT) and Power Processing Unit has centered on two primary areas of investigation: integration of porous-tungsten dispenser-type cathode inserts into the thruster (replacing previous inserts of rolled-tantalum-foil design) and characterization of thruster operation with the new inserts. Characterization testing of the EMT and of the new cathodes has demonstrated acceptable thruster performance and cathode ignition parameters; the only perceived change in thuster performance has been that a small amount of cathode heater power is required to maintain nominal keeper voltages. Thermal modeling of the cathode structures has facilitated design revisions which reduce this power requirement.

Williamson, W. S.

Characteristics of the LeRC/Hughes J-series 30-cm engineering model thruster

As a consequence of endurance and structural tests performed on 900-series engineering model thrusters (EMT), several modifications in design were found to be necessary for achieving performance goals. The modified thruster is known as the J-series EMT. The most important of the design modifications affect the accelerator grid, gimbal mount, cathode polepiece, and wiring harness. The paper discusses the design modifications incorporated, the condition(s) they corrected, and the characteristics of the modified thruster.

Collett, C. R.

Modeling composite and fluffy grains: The effects of porosity

Recent studies of interplanetary and interstellar dust provide evidence that cosmic dust grains are fluffy, composite objects, highlighting the need for models of the electromagnetic scattering by these grains. Effective medium theory (EMT) with Mie-type series solutions has been used to explore the effects of porosity which would be important in composite dust particles. While this indirect approach is both flexible and computationally efficient, it is not necessarily a good approximation. The need for EMT and its rather restrictive assumptions may be circumvented through a direct computation of the scattering properties via finite element methods, such as the discrete dipole approximation (DDA). Recently, the utility of the DDA method has been advanced significantly through improvements in theory, in numerical algorithms, and in computer hardware. Extensive calculations with the DDA method are used here to examine more directly the effects of porosity. A particular emphasis is placed upon developing a valid methodology. For both solid and porous targets we establish both numerical and physical convergence properties over the range of size parameter that is required for our study. DDA cross sections for grains with a range of porosity are compared to those computed by the EMT/series expansion technique to examine the applicability of several mixing rules, including two extensions of the Bruggeman rule. We show that for particles with Rayleigh vacuum inclusions, the extension proposed by Rouleau & Martin is quite successful. We also investigate the effects of larger, non-Rayleigh vacuum inclusions for various levels of porosity and find that they can be significant.

Wolff, Michael J.

Transmission Measurement of the Third-Order Susceptibility of Gold

Gold nanoparticle composites are known to display large optical nonlinearities. In order to assess the validity of generalized effective medium theories (EMT's) for describing the linear and nonlinear optical properties of metal nanoparticle composites, knowledge of the linear and nonlinear susceptibilities of the constituent materials is a prerequisite. In this study the inherent nonlinearity of the metal is measured directly (rather than deduced from a suitable EMT) using a very thin gold film. Specifically, we have used the z-scan technique at a wavelength near the transmission window of bulk gold to measure the third-order susceptibility of a continuous thin gold film deposited on a quartz substrate surface-modified with a self-assembled monolayer to promote adhesion and uniformity without affecting the optical properties. We compare our results with predictions which ascribe the nonlinear response to a Fermi-smearing mechanism. Further, we note that the sign of the nonlinear susceptibility is reversed from that of gold nanoparticle composites.

Smith, David D.

Control logic for a 30 cm diameter ion thruster

Tests with a 30 cm thruster having EMT critical components have defined start-up criteria. Of the three phases comprising the start-up mode, the preheat phase is intended to heat critical feed system components to desired temperatures. Of the seven supplies available during the preheat phase, only three were found to be useful for purposes of preheating the thruster. These were the cathode and neutralizer tip heater supplies and the isolator heater supply. If the needed temperatures are attained during preheat, the ignition phase requires only several seconds. The heat phase serves to establish the proper propellant flow rates prior to the transition to the run mode. Using these techniques, a thruster with initial temperatures in the range of -15 to +25 C can be reliably started and provide a 1.0 amp beam within 45 min. High voltage recycle time profiles indicate limitations on several time functions.

Bechtel, R. T.

Emergency medical services

When NASA was established in 1958, it was known that space flight would require efforts beyond those of NASA to ensure the health and safety of our astronauts. On 10 Aug. 1958, a Secretary of Defense memorandum was signed that assigned the first Department of Defense (DOD) Manager to provide support to NASA for Project Mercury. This established a chain of command through the Joint Chiefs of Staff to the Secretary of Defense. The current charter is dated 19 Mar. 1986 and assigns the DOD Manager responsibilities to the Commander and Chief, US Space Command. The DOD Managers charter has many support areas and among them are recovery of astronauts and medical support. Today these efforts support the Space Shuttle and Space Station Programs. Briefly, the program works with each organization tasking the other through a requirements document. Level of care, communications, and recovery requirements are established; NASA and the DOD provide the capability to meet them. NASA is also responsible for the specialized training and equipment needed to meet these requirements. A Shuttle launch a KSC requires an Emergency Medical Services (EMS) coordinator on console to facilitate communications, ensure proper coverage, and coordinate with area hospitals. A contingent of NASA medical personnel are assembled to provide triage and medical support capabilities. The DOD provides medical evacuation (MEDEVAC) helicopters with surgeons and pararescue specialists (PJ's) or emergency medical technicians (EMT's). Each helicopter is equipped with at least one doctor and one PJ/EMT per astronaut crew member. Transoceanic abort landing (TAL) sites and end of mission (EOM) sites have similar structures, with TAL sites utilizing fixed wingg aircraft for MEDEVAC. The DOD also supports contingency planning for the support and return of crew members from the Space Station Freedom. Much of this support has been directed at the recovery of crew members following the landing of an Assured Crew Return Vehicle.

Billica, Roger

NSTAR Ion Propulsion System Power Electronics

The NASA Solar Electric Propulsion Technology Application Readiness (NSTAR) program, managed by the Jet Propulsion Laboratory (JPL), is currently developing a high performance, simplified ion propulsion system. This propulsion system, which is throttleable from 0.5- to 2.3-kW output power to the thruster, targets primary propulsion applications for planetary and Earth-space missions and has been baselined as the primary propulsion system for the first New Millennium spacecraft. The NASA Lewis Research Center is responsible for the design and delivery of a breadboard power processing unit (PPU) and an engineering model thruster (EMT) for this system and will manage the contract for the delivery of the flight hardware to JPL. The PPU requirements, which dictate a mass of less than 12 kg with an efficiency of 0.9 or greater at a 2.3-kW output, forced a departure from the state-of-the-art ion thruster PPU design. Several innovations--including dual-use topologies, simplified thruster control, and the use of ferrite magnetic materials--were necessary to meet these requirements.

Source record

Preliminary Spectroscopic Measurements for a Gallium Electromagnetic (GEM) Thruster

As a propellant option for electromagnetic thrusters, liquid ,gallium appears to have several advantages relative to other propellants. The merits of using gallium in an electromagnetic thruster (EMT) are discussed and estimates of discharge current levels and mass flow rates yielding efficient operation are given. The gallium atomic weight of 70 predicts high efficiency in the 1500-2000 s specific impulse range, making it ideal for higher-thrust, near-Earth missions. A spatially and temporally broad spectroscopic survey in the 220-520 nm range is used to determine which species are present in the plasma and estimate electron temperature. The spectra show that neutral, singly, and doubly ionized gallium species are present in a 20 J, 1.8 kA (peak) are discharge. With graphite present on the insulator to facilitate breakdown, singly and doubly ionized carbon atoms are also present, and emission is observed from molecular carbon (CZ) radicals. A determination of the electron temperature was attempted using relative emission line data, and while the spatially and temporally averaged, spectra don't fit well to single temperatures, the data and presence of doubly ionized gallium are consistent with distributions in the 1-3 eV range.

Thomas, Robert E.