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All sourcesNASA NTRSDOE OSTI
DOE OSTI2018

R&D Roadmap for Enhanced Resilient Plant Systems, Metrics, Scenarios, Risk Analysis, and Modeling and Simulation

The nuclear industry and the U.S. Department of Energy are jointly developing accident tolerant fuel (ATF) which has the potential to greatly improve the safety and economic performance of the current fleet of NPPs. The combination of ATF with the optimal use of FLEX equipment (deployed as an industry response to the Fukushima Daiichi Nuclear Power Plant (NPP) accident in 2012), enhancements on plant components and systems, incorporation of augmented cooling systems or new passive cooling systems, and improvements in fuel cycle efficiency, have the potential to render the NPPs more efficient and resilient to events (both internally and externally initiated) that have the potential to challenge the integrity of the fuel or plant containment. In this report, combinations of these plant improvements are called Enhanced Resilient Plant Systems. In order to accelerate the development and deployment of the components that comprise Enhanced Resilient Plant Systems, a well-integrated toolkit that is fuel/clad-, fuel cycle-, systems-, and scenario-centric is required to provide a ready platform for the analysis of the advanced ATF fuel and cladding materials that are being developed, as well as plant systems modifications to achieve enhanced resiliency. Since the deployment of these enhancements will comprise a risk/benefit tradeoff of their impacts on plant safety and economics, the envisioned toolkit will need to be capable to characterizing the multidimensional aspects associated with these evaluations and provide decision-makers with information that can optimize the technological, economic, and safety benefits that are to be obtained. The Risk-Informed Systems Analysis (RISA) Pathway of the DOE Light Water Reactor Sustainability (LWRS) Program initiated several research tasks to develop analytical capabilities to support the industry in the transition to the ATF and Enhanced Resilient Plant Systems. The general idea behind the initiative is the development of an integrated evaluation approach which combines the plant probabilistic risk assessment (PRA) models with Multi-Physics Best Estimate Plus Uncertainty (MP-BEPU) analyses in a seamless fashion. The integrated evaluation framework aims at enabling plant system configuration variations to be studied with speed and precision, including detailed risk and benefit assessments of introducing ATF and Enhanced Resilient Plant Systems into current LWR plants to achieve both safety and operational performance enhancements. The focus of this report is to present an integrated research and development (R&D) roadmap to identify and perform high value evaluations of proposed ATF and Enhanced Resilient Plant Systems concepts to identify both the technical (e.g. benefits to risk, safety, and operational margins) and the economic (i.e. business and cost) elements associated with industry adoption of the technology. The integrated evaluation approach will be developed to support the development, testing, qualification, licensing, and deployment of ATF and Enhanced Resilient Plant Systems technology that is capable of achieving substantial safety and economic improvements as well as timely widespread adoption by the US nuclear industry.

22 GENERAL STUDIES OF NUCLEAR REACTORS↗
DOE OSTI2024

The 38 th Annual Interdisciplinary Plant Group Symposium: Enhancing the Resilience of Plant Systems to Climate Change (Meeting Summary)

The University of Missouri’s Interdisciplinary Plant Group held its 38th annual symposium on May 25–27. In short, it was a resounding success! The air was almost electric because of a confluence of events that were particular to this symposium. Not only was there great enthusiasm from students attending their first ever scientific meeting, but this was coupled with excitement and delight exuded by many of our senior invited speakers, who were attending their first in-person meeting since the Covid pandemic began. After a long planning period, and with the ongoing pandemic injecting uncertainty, it was gratifying to see the symposium unfold with such excitement and engagement among participants. The symposium was organized to address the grand challenge of “Enhancing the Resilience of Plant System to Climate Change”, by bringing together experts at the intersections of multiple disciplines to educate our community on the opportunities to develop innovative mitigation strategies and to adapt plants to climate change.

54 ENVIRONMENTAL SCIENCES↗
DOE OSTI2018

Plant-Level Scenario-Based Risk Analysis for Enhanced Resilient PWR – SBO and LBLOCA

This report documents the activities performed during the FY2018 for the DOE Light Water Reactor Sustainability (LWRS) Program, Risk-Informed System Analysis (RISA) Pathway, Enhanced Resilient Plant (ERP) Systems research. The purpose of the RISA Pathway research and development is to support plant owner-operator decisions with the aim to improve the economics, reliability, and maintain the high levels of safety of current nuclear power plants over periods of extended plant operations. The concept of ERP refers to the combinations of Accident Tolerant Fuel (ATF), optimal use of Diverse and Flexible Coping Strategy (FLEX), enhancements to plant components and systems, and the incorporation of augmented or new passive cooling systems, as well as improved fuel cycle efficiency. The objective of the ERP research effort is to use the RISA methods and toolkit in industry applications, including methods development and early demonstration of technologies, in order to enhance existing reactors safety features (both active and passive) and to substantially reduce operating costs through risk-informed approaches to plant design modifications to the plant and their characterization. There are two main focus areas in FY2018 for the ERP R&D efforts. One is to evaluate the risk impact brought by the ATF designs and FLEX in the selected accident scenarios. The other is to investigate various approaches to accomplish the ERP research objective, i.e., use RISA methods and toolkit to enhance existing reactors safety features and reduce plant operating costs.

99 GENERAL AND MISCELLANEOUS↗
DOE OSTI2023

Safety Analysis of FeCrAl Accident-Tolerant Fuels with Increased Enrichment and Extended Burnup

The U.S. nuclear industry is facing a strong challenge to maintain regulatory-required levels of safety while ensuring economic competitiveness to stay in business. Safety remains a key parameter for all aspects related to the operation of light water reactor nuclear power plants (NPPs), and it can be achieved more economically by using a risk-informed ecosystem, such as that being developed by the Risk-Informed Systems Analysis Pathway under the U.S. Department of Energy Light Water Reactor Sustainability Program. This program is promoting a wide range of research and development activities to maximize both the safety and economically efficient performance of NPPs through improved scientific understanding, especially given that many plants are considering a second license renewal. The Risk-Informed Systems Analysis Pathway has two main goals: (1) The deployment of methodologies and technologies that enable a better representation of the safety margins and factors that contribute to cost and safety, and (2) The development of advanced applications that enable cost-effective plant operation. As part of this pathway, the Enhanced Resilient Plant project refers to an NPP where safety is improved by implementing various measures, such as accident-tolerant fuels, diverse and flexible coping strategies, enhancements to plant components and systems, incorporation of augmented or new passive cooling systems, and utilization of advanced battery technologies. The objective of the Enhanced Resilient Plant project is to use novel methods and computational tools to enhance existing reactors’ safety while reducing operational costs. This report documents research and development conducted in support of deployment of accident-tolerant fuels. This project performed safety analyses for the steady-state normal operation, anticipated operational occurrences, and design-basis accidents of a representative four-loop pressurized water reactor model with Zr and FeCrAl accident-tolerant fuel clad with higher enrichment and burnup supporting plant refueling cycles of 18 and 24 months. The source terms and environmental impacts were studied for a large-break loss of coolant accident, including uncertainty analyses.

11 NUCLEAR FUEL CYCLE AND FUEL MATERIALS↗
DOE OSTI2021

Risk-Informed Analysis for Enhanced Resilient Nuclear Power Plant with Initiatives including ATF, FLEX, and Advanced Battery Technology

This report documents the activities performed by the Idaho National Laboratory (INL) during fiscal year (FY) 2021 for the U.S. Department of Energy (DOE) Light Water Reactor Sustainability (LWRS) Program, Risk-Informed Systems Analysis (RISA) Pathway, Enhanced Resilient Plant (ERP) Systems research. The purpose of the RISA Pathway research and development is to support plant owner-operator decisions with the aim to improve the economics, reliability, and maintain the high levels of safety of current nuclear power plants over periods of extended plant operations. The concept of ERP refers to the combinations of accident-tolerant fuel (ATF), optimal use of diverse and flexible coping strategy (FLEX), enhancements to plant components and systems, the incorporation of augmented or new passive cooling systems, the advanced battery technology with extended capacity, as well as improved fuel cycle efficiency. The objective of the ERP research effort is to use the RISA methods and toolkit in industry applications, including methods development and early demonstration of technologies, in order to enhance existing reactors? safety features and to substantially reduce operating costs through risk-informed approaches to plant design modifications to the plant and their characterization. The ERP R&D efforts in FY 2021 are focused on three industry initiatives, including accident-tolerant fuel (ATF), optimal use of diverse and flexible coping strategy (FLEX), and advanced battery technology with extended capacity.

99 GENERAL AND MISCELLANEOUS↗
DOE OSTI2023

Assessment of Modeling and Simulation Technical Gaps in Safety Analysis of High Burnup Accident-Tolerant Fuels

The United States nuclear industry is facing a strong challenge to maintain regulatory-required levels of safety while ensuring economic competitiveness to stay in business. Safety remains a key parameter for all aspects related to the operation of light water reactor (LWR) nuclear power plants (NPPs), and it can be achieved more economically by using a risk-informed ecosystem, such as that being developed by the Risk-Informed Systems Analysis (RISA) Pathway under the U.S. Department of Energy (DOE) Light Water Reactor Sustainability (LWRS) Program. The LWRS Program is promoting a wide range of research and development activities to maximize both the safety and economically efficient performance of NPPs through improved scientific understanding, especially given that many plants are considering second license renewal. The RISA Pathway has two main goals: The deployment of methodologies and technologies that enable better representation of safety margins and the factors that contribute to cost and safety, and; The development of advanced applications that enable cost-effective plant operation. As part of the RISA Pathway, the Enhanced Resilient Plant (ERP) project refers to an NPP where safety is improved by implementing various measures, such as accident-tolerant fuels (ATF), diverse and flexible coping strategy (FLEX), enhancements to plant components and systems, incorporation of augmented or new passive cooling systems, and utilization of advanced battery technologies. The objective of the ERP research is to use novel methods and computational tools to enhance existing reactors’ safety while reducing operational costs. Many U.S. utilities are targeting implementation of ATFs instead of traditional fuel in the near future since ATFs offer benefits in terms of improved performance and cost savings. The robust properties of ATF make it possible to extend the refueling cycle from 18 to 24 months in addition to the opportunity to use less of fuel. Extensive safety assessments are required to support regulatory requirements and obtain the approvals to use ATFs and the ERP project support the industry by developing novel effective methodologies for safety evaluations. In this project, the technical gaps in the modeling and simulation of the high burnup (HBU) ATF were assessed in terms of the fuel cladding behavior during the postulated accident events. The issues were identified in modeling the cladding deformation, the hydrodynamic change due to cladding deformation and the critical heat flux (CHF). The RELAP5-3D cladding deformation model was assessed by multiple verification tests and validation with the instrumented fuel assembly (IFA) experiment.

11 NUCLEAR FUEL CYCLE AND FUEL MATERIALS↗
DOE OSTI2019

Risk-Informed Analysis for an Enhanced Resilient PWR with ATF, FLEX, and Passive Cooling

This report documents the activities performed by Idaho National Laboratory (INL) during the fiscal year (FY) 2019 for the DOE Light Water Reactor Sustainability (LWRS) Program, Risk-Informed System Analysis (RISA) Pathway, Enhanced Resilient Plant (ERP) Systems research. The purpose of the RISA Pathway research and development is to support plant owner-operator decisions with the aim to improve the economics, reliability, and maintain the high levels of safety of current nuclear power plants over periods of extended plant operations. The concept of ERP refers to the combinations of Accident Tolerant Fuel (ATF), optimal use of Diverse and Flexible Coping Strategy (FLEX), enhancements to plant components and systems, and the incorporation of augmented or new passive cooling systems, as well as improved fuel cycle efficiency. The objective of the ERP research effort is to use the RISA methods and toolkit in industry applications, including methods development and early demonstration of technologies, in order to enhance existing reactors safety features (both active and passive) and to substantially reduce operating costs through risk-informed approaches to plant design modifications to the plant and their characterization. The FY 2019 efforts documented in this report is an extension of those conducted in FY 2018. The same analysis process, risk analysis approaches, and analysis tools as in FY 2018 are used for near-term ATF cladding [i.e., Iron-Chromium-Aluminum (FeCrAl) cladding and Chromium (Cr) coated cladding] designs under the postulated loss of feedwater (LOFW) and steam generator tube rupture (SGTR) accident scenarios. In addition, FLEX analysis was presented for an overview of FLEX equipment and strategies implemented in nuclear industry after Fukushima accident. A FLEX model was developed and incorporated into the generic SAPHIRE model to assess the risk impact from FLEX. FLEX HRA analysis was performed which suggested an approach to HRA with FLEX strategies. Passive cooling system analysis includes an overview of the DNC system, the RELAP5-3D simulations of the DNC system in selected SBO scenarios, and the evaluation of the DNC system risk impact using the generic PRA model.

99 GENERAL AND MISCELLANEOUS↗
DOE OSTI2019

Evaluation of the Benefits of ATF, FLEX, and Passive Cooling System for an Enhanced Resilient PWR Model

This report, along with report INL/EXT-19-53556, documents the activities performed by Idaho National Laboratory (INL) during the fiscal year (FY) 2019 for the DOE Light Water Reactor Sustainability (LWRS) Program, Risk-Informed System Analysis (RISA) Pathway, Enhanced Resilient Plant (ERP) Systems research. This report includes additional accident scenarios for risk-informed ATF analysis, and examines the risk impacts of FLEX and passive cooling system on nuclear power plants (NPPs) from additional perspectives. The same analysis process, risk analysis approaches, and analysis tools as in FY 2018 were used for near-term ATF cladding (i.e., Iron-Chromium-Aluminum [FeCrAl] cladding and Chromium [Cr]-coated cladding) designs under the small break loss-of-coolant accident (SBLOCA) scenarios, two types of general transient scenarios (locked rotor and turbine trip), and main steam line break (MSLB) scenarios. Benchmark calculations for ATF analysis using RELAP5-3D and MELCOR are also presented. In addition, based on the generic probabilistic risk assessment (PRA) model, the risk impacts of FLEX and the dynamic natural convection (DNC) system on NPPs are evaluated through a risk-informed decision-making process, i.e., significance determination process (SDP).

99 GENERAL AND MISCELLANEOUS↗
DOE OSTI2020

A Multi-Criteria Impact Evaluation Methodology Applied to FLEX Strategies in Nuclear Power Plants

As responses to Fukushima Dai-ichi nuclear accident, several advanced, near-term technologies have been or are being introduced to enhance safety of operating nuclear power plants (NPPs) as well as improving their sustainability. Examples include Accident-Tolerant Fuel designs, Diverse and Flexible Coping Strategies (FLEX), and passive cooling system designs. The combinations of these technologies, coupled with enhancements to plant components and systems and improved fuel cycle efficiency, constitute a concept of Enhanced Resilient Plant Systems, which is proposed and being explored by Idaho National Laboratory (INL) under the Risk-Informed Systems Analysis Pathway of the U.S. Department of Energy’s Light Water Reactor Sustainability Program. To decide if introducing a technology is necessary or worthy, the impacts of this technology on NPPs should be evaluated, preferably in a quantitative manner, and provided to decision makers. This paper summarizes an on-going work conducted by the authors on developing a multi-criteria impact evaluation methodology. This methodology is expected to be applicable to evaluate a variety of technologies, while FLEX strategy is adopted as a case study for this paper.

99 GENERAL AND MISCELLANEOUS↗
DOE OSTI2025

A stable 15-member bacterial SynCom promotes Brachypodium growth under drought stress

Introduction: Rhizosphere microbiomes are known to drive soil nutrient cycling and influence plant fitness during adverse environmental conditions. Field-derived robust Synthetic Communities (SynComs) of microbes mimicking the diversity of rhizosphere microbiomes can greatly advance a deeper understanding of such processes. However, assembling stable, genetically tractable, reproducible, and scalable SynComs remains challenging. Methods: Here, we present a systematic approach using a combination of network analysis and cultivation-guided methods to construct a 15-member SynCom from the rhizobiome of Brachypodium distachyon. This SynCom incorporates diverse strains from five bacterial phyla. Genomic analysis of the individual strains was performed to reveal encoded plant growth-promoting traits, including genes for the synthesis of osmoprotectants (trehalose and betaine) and Na+/K+ transporters, and some predicted traits were validated by laboratory phenotypic assays. Results: The SynCom demonstrates strong stability both in vitro and in planta. Most strains encoded multiple plant growth-promoting functions, and several of these were confirmed experimentally. The presence of osmoprotectant and ion transporter genes likely contributed to the observed resilience of Brachypodium to drought stress, where plants amended with the SynCom recovered better than those without. We further observed preferential colonization of SynCom strains around root tips under stress, likely due to active interactions between plant root metabolites and bacteria. Discussion: Our results demonstrate that trait-informed construction of synthetic communities can yield stable, functionally diverse consortia that enhance plant resilience under drought. Preferential colonization near root tips points to active, localized plant-microbe signaling as a component of stress-responsive recruitment. This stable SynCom provides a scalable platform for probing mechanisms of plant-microbe interaction and for developing microbiome-based strategies to improve soil and crop performance in variable environments.

Yadav, Archana↗
DOE OSTI2020

Risk-Informed ATF and FLEX Analysis for an Enhanced Resilient BWR Under Design-Basis and Beyond-Design-Basis Accidents

This report documents the activities performed by the Idaho National Laboratory (INL) during fiscal year (FY) 2020 for the U.S. Department of Energy (DOE) Light Water Reactor Sustainability (LWRS) Program, Risk-Informed System Analysis (RISA) Pathway, Enhanced Resilient Plant (ERP) Systems research. The purpose of the RISA Pathway research and development is to support plant owner-operator decisions with the aim to improve the economics, reliability, and maintain the high levels of safety of current nuclear power plants over periods of extended plant operations. The concept of ERP refers to the combinations of accident-tolerant fuel (ATF), optimal use of diverse and flexible coping strategy (FLEX), enhancements to plant components and systems, and the incorporation of augmented or new passive cooling systems, as well as improved fuel cycle efficiency. The objective of the ERP research effort is to use the RISA methods and toolkit in industry applications, including methods development and early demonstration of technologies, in order to enhance existing reactors’ safety features (both active and passive) and to substantially reduce operating costs through risk-informed approaches to plant design modifications to the plant and their characterization. One main focus of the FY 2020 efforts documented in this report was to extend the analyses conducted in FYs 2018 and 2019 for a pressurized water reactor (PWR) to a boiling water reactor (BWR). The same analysis process, risk analysis approaches, and analysis tools as in the previous work for PWR were used for a generic BWR with near-term ATF cladding (i.e., Iron-Chromium-Aluminum [FeCrAl] cladding and Chromium [Cr]-coated cladding) designs under the postulated station blackout (SBO) and medium loss-of-coolant (MLOCA) accident scenarios. In addition, a FLEX model was developed and incorporated into a generic BWR probabilistic risk assessment (PRA) model using the INL-developed software tool, Systems Analysis Programs for Hands-on Integrated Reliability Evaluations (SAPHIRE), to assess the risk impact from FLEX. The other main focus of the FY 2020 efforts was to advance analysis methods, including developing dynamic approach for FLEX human reliability analysis (HRA) using the INL-developed software tool, Event Modeling Risk Assessment using Linked Diagrams (EMRALD), as well as developing a multicriterion benefit evaluation (MCBE) method for evaluating costs and benefits of safety enhancements in nuclear power plants (NPPs). As a case study, the MCBE method was applied to evaluate the costs and benefits brought by FLEX implementation.

99 GENERAL AND MISCELLANEOUS↗
DOE OSTI2024

Halophytes and heavy metals: A multi‐omics approach to understand the role of gene and genome duplication in the abiotic stress tolerance of Cakile maritima

Abstract Premise The origin of diversity is a fundamental biological question. Gene duplications are one mechanism that provides raw material for the emergence of novel traits, but evolutionary outcomes depend on which genes are retained and how they become functionalized. Yet, following different duplication types (polyploidy and tandem duplication), the events driving gene retention and functionalization remain poorly understood. Here we usedCakile maritima, a species that is tolerant to salt and heavy metals and shares an ancient whole‐genome triplication with closely related salt‐sensitive mustard crops (Brassica), as a model to explore the evolution of abiotic stress tolerance following polyploidy. Methods Using a combination of ionomics, free amino acid profiling, and comparative genomics, we characterize aspects of salt stress response inC. maritimaand identify retained duplicate genes that have likely enabled adaptation to salt and mild levels of cadmium. Results Cakile maritimais tolerant to both cadmium and salt treatments through uptake of cadmium in the roots. Proline constitutes greater than 30% of the free amino acid pool inC. maritimaand likely contributes to abiotic stress tolerance. We find duplicated gene families are enriched in metabolic and transport processes and identify key transport genes that may be involved inC. maritimaabiotic stress tolerance. Conclusions These findings identify pathways and genes that could be used to enhance plant resilience and provide a putative understanding of the roles of duplication types and retention on the evolution of abiotic stress response.

Plant Sciences↗
DOE OSTI2025

Plant genotype and rhizobia strain combinations strongly influence the transcriptome under heavy metal stress conditions in Medicago truncatula

Heavy metals such as cadmium (Cd) and mercury (Hg) pose significant threats to plant health and food safety as they are absorbed from the environment. Legumes are generally considered sensitive to heavy metals but possess standing genetic variation for accumulation and tolerance to toxic ions. We conducted a transcriptomic analysis on hydroponically and soil grown Medicago truncatula plants to investigate gene expression responses to Cd and Hg exposure in roots, leaves, and nodules. By using plant genotypes with varying metal tolerance or accumulation levels, we observed distinct clustering of gene ontologies, indicating tissue-specific, genotype-specific, and metal-specific gene expression patterns. Considering the symbiotic relationship between legumes and nitrogen-fixing bacteria, we further examined plant phenotypes and transcriptomes of plant genotypes with contrasting Hg accumulation levels and inoculated them with high or low Hg-tolerant Sinorhizobium medicae strains that have presence-absence variation for a mercury reductase (Mer) operon. Host plants inoculated with the Hg-tolerant rhizobia strain possessing a Mer operon exhibited less reduction in nodule number and plant biomass. A smaller reduction in iron (Fe) distribution in nodules after Hg stress was measured using X-ray Fluorescence (XRF) imaging. Dual transcriptome (host plant and bacteria) analysis of nodules revealed a remarkable decrease in the number of differentially expressed genes (DEGs) and clustering of gene ontologies in plants inoculated with the Hg-tolerant rhizobia strain, including symbiosis related genes. This finding suggests that the Hg-tolerant rhizobia strain has the potential to mitigate Hg stress in host plants. Furthermore, we observed genotype by-genotype interactions between the high Hg accumulating plant genotype and the Hg-tolerant rhizobia strain. These findings provide insights into enhancing plant resilience in contaminated environments through optimizing legume-rhizobia interactions for heavy metal tolerance.

59 BASIC BIOLOGICAL SCIENCES↗
DOE OSTI2026

Desorption Electrospray Ionization–Mass Spectrometry Imaging Provides Spatiochemical Information on Potential Biocontrol Agents against Phytophthora capsici Infection in Tomato Plants

Biological control agents can offer an eco-friendly and more sustainable alternative to conventional chemical pesticides, providing protection against destructive pathogens, such as Phytophthora capsici, while reducing potential environmental harm associated with synthetic pesticide use in agricultural systems. This work evaluates the biocontrol effectiveness of Bacillus vallismortis, Bacillus amyloliquefaciens, Bacillus thuringiensis, and Bacillus subtilis, against the widespread plant pathogen Phytophthora capsici. Our studies showed that Bacillus thuringiensis and Bacillus subtilis promote plant growth and provide protection against Phytophthora capsici in both in vitro and in vivo greenhouse studies, while Bacillus vallismortis and Bacillus amyloliquefaciens were effective in vitro but not in vivo. Specifically, Bacillus thuringiensis was observed to both hinder the growth of Phytophthora capsici and enhance plant resilience to this pathogen, with B. thuringiensis-treated, pathogen-exposed plants displaying a 94.4% increase in root length and a 74.0% increase in shoot height compared to plants with only oomycete exposure. To probe the molecular interactions between the biocontrol agent and pathogen, a dual culture of Bacillus thuringiensis and Phytophthora capsici was analyzed in situ using a desorption electrospray ionization–mass spectrometry imaging (DESI-MSI) workflow. This approach interrogated the spatially oriented biochemical interactions that may serve as the molecular foundation for the effectiveness of these biological control agents in crop protection, identifying seven unique phenotypic regions within the dual culture. Herein, we demonstrate the benefits of biological control agent application in tomato cultivation and showcase the strengths of desorption electrospray ionization–mass spectrometry imaging when applied to the spatially resolved molecular characterization of agriculturally relevant microorganisms.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗
DOE OSTI2026

Small Hydro Power Plants with Integrated BESS for Enhance Resiliency

Battery energy storage systems (BESS) are an important asset for power systems with high integration levels of renewable energy, and they can be controlled to provide various services to the grid. This paper presents the hardware demonstration and characterization of using a utility-scale BESS with grid-following (GFL) and grid-forming (GFM) controls and a run-of-river (ROR) hydropower plant to perform a bottom-up power system black start that enhances power systems' resiliency. ROR hydropower plants are generally not used for power system restoration due to their frequency instability during islanded operation; however, BESS with droop control can provide critical damping to convert a ROR hydropower generator into a black start- capable unit. To demonstrate this, we carry out hardware experiments at the megawatt-scale integrating a synchronous generator driven by a dynamometer, an actual GFL/GFM BESS, a medium-voltage impedance network, and a load bank. The demonstration shows the different roles of BESS with GFL and GFM control in power system restoration. GFL BESS can suffer from high-frequency oscillations, even instability, depending on the droop control gain and loading condition. The results provide further insights for system operators on how GFL- or GFM controlled BESS can enhance grid stability and how hydro-BESS hybrids can operate as a black-start-capable unit. The presented experimental results are also a valuable resource to understand the different stability characteristics of real-world BESS with different control modes.

24 POWER TRANSMISSION AND DISTRIBUTION↗
DOE OSTI2023

Stressing the importance of plant specialized metabolites: omics-based approaches for discovering specialized metabolism in plant stress responses

Plants produce a diverse range of specialized metabolites that play pivotal roles in mediating environmental interactions and stress adaptation. These unique chemical compounds also hold significant agricultural, medicinal, and industrial values. Despite the expanding knowledge of their functions in plant stress interactions, understanding the intricate biosynthetic pathways of these natural products remains challenging due to gene and pathway redundancy, multifunctionality of proteins, and the activity of enzymes with broad substrate specificity. In the past decade, substantial progress in genomics, transcriptomics, metabolomics, and proteomics has made the exploration of plant specialized metabolism more feasible than ever before. Notably, recent advances in integrative multi-omics and computational approaches, along with other technologies, are accelerating the discovery of plant specialized metabolism. In this review, we present a summary of the recent progress in the discovery of plant stress-related specialized metabolites. Emphasis is placed on the application of advanced omics-based approaches and other techniques in studying plant stress-related specialized metabolism. Additionally, we discuss the high-throughput methods for gene functional characterization. These advances hold great promise for harnessing the potential of specialized metabolites to enhance plant stress resilience in the future.

59 BASIC BIOLOGICAL SCIENCES↗
DOE OSTI2024

Elevated Temperature Effects on Protein Turnover Dynamics in Arabidopsis thaliana Seedlings Revealed by 15 N-Stable Isotope Labeling and ProteinTurnover Algorithm

Global warming poses a threat to plant survival, impacting growth and agricultural yield. Protein turnover, a critical regulatory mechanism balancing protein synthesis and degradation, is crucial for the cellular response to environmental changes. We investigated the effects of elevated temperature on proteome dynamics in Arabidopsis thaliana seedlings using 15 N-stable isotope labeling and ultra-performance liquid chromatography-high resolution mass spectrometry, coupled with the ProteinTurnover algorithm. Analyzing different cellular fractions from plants grown under 22 °C and 30 °C growth conditions, we found significant changes in the turnover rates of 571 proteins, with a median 1.4-fold increase, indicating accelerated protein dynamics under thermal stress. Notably, soluble root fraction proteins exhibited smaller turnover changes, suggesting tissue-specific adaptations. Significant turnover alterations occurred with redox signaling, stress response, protein folding, secondary metabolism, and photorespiration, indicating complex responses enhancing plant thermal resilience. Conversely, proteins involved in carbohydrate metabolism and mitochondrial ATP synthesis showed minimal changes, highlighting their stability. This analysis highlights the intricate balance between proteome stability and adaptability, advancing our understanding of plant responses to heat stress and supporting the development of improved thermotolerant crops.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗