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At least 19 records

Hindlimb Suspension (HLS) in Rodents for the Study of Intracranial Pressure, Molecular and Histologic Changes in the Eye, and CSF Production Regulation and Resorption: A Status Report of Two Studies

This status report corresponds to two studies tied to an animal experiment being executed at the University of California Davis (Charles Fuller's laboratory). The animal protocol uses the well-documented rat hindlimb suspension (HLS) model, to examine the relationship between cephalic fluid shifts and the regulation of intracranial (ICP) and intraocular (IOP) pressures as well as visual system structure and function. Long Evans rats are subjected to HLS durations of 7, 14, 28 and 90 days. Subgroups of the 90-day animals are studied for recovery periods of 7, 14, 28 or 90 days. All HLS subjects have age-matched cage controls. Various animal cohorts are planned for this study: young males, young females and old males. In addition to the live measures (ICP by telemetry, IOP and retinal parameters by optical coherence tomography) which are shared with the Fuller study, the specific outcomes for this study include: -Gene expression analysis of the retina -Histologic analysis - Analysis of the microvasculature of retina flat mounts by NASA's VESsel GENeration Analysis (VESGEN) Software. To date, the young male and female cohorts are being completed. Due to the need to keep technical variation to a minimum, the histologic and genomic analyses have been delayed until all samples from each cohort are available and can be processed in a single batch per cohort. The samples received so far correspond to young males sacrificed at 7,14, 28 and 90 days of HLS and at 90 days of recovery; and from young females sacrificed at 7, 14 and 28 of HLS. A complementary study titled: "A gene expression and histologic approach to the study of cerebrospinal fluid (CSF) production and outflow in hindlimb suspended rats" seeks to study the molecular components of CSF production and outflow modulation as a result of HLS, bringing a molecular and histologic approach to investigate genome wide expression changes in the arachnoid villi and choroid plexus of HLS rats compared to rats in normal posture.

Theriot, C. A.

A Method for Landsat and Sentinel 2 (HLS) BRDF Normalization

The Harmonized Landsat/Sentinel-2 (HLS) project aims to generate a seamless surface reflectance product by combining observations from USGS/NASA Landsat-8 and ESA Sentinel-2 remote sensing satellites. These satellites’ sampling characteristics provide nearly constant observation geometry and low illumination variation through the scene. However, the illumination variation throughout the year impacts the surface reflectance by producing higher values for low solar zenith angles and lower reflectance for large zenith angles. In this work, we present a model to derive the bidirectional reflectance distribution function (BRDF) normalization and apply it to the HLS product at 30m spatial resolution. It is based on the BRDF parameters estimated from the MODerate Resolution Imaging Spectroradiometer (MODIS) surface reflectance product (M{O,Y}D09) at 1 km spatial resolution using the VJB method (Vermote et al., 2009). Unsupervised classification (segmentation) of HLS images is used to disaggregate the BRDF parameters to the HLS spatial resolution and to build a BRDF parameters database at HLS scale. We first test the proposed BRDF normalization for different solar zenith angles over two homogeneous sites, in particular one desert and one Peruvian Amazon forest. The proposed method reduces both the correlation with the solar zenith angle and the coefficient of variation (CV) of the reflectance time series in the red and near infrared bands to 4% in forest and keeps a low CV of 3% to 4% for the deserts. Additionally, we assess the impact of the view zenith angle (VZA) in an area of the Brazilian Amazon forest close to the equator, where impact of the angular variation is stronger because it occurs in the principal plane. The directional reflectance shows a strong dependency with the VZA. The current HLS BRDF correction reduces this dependency but still shows an under-correction, especially in the near infrared, while the proposed method shows no dependency with the view angles. We also evaluate the BRDF parameters using field surface albedo measurements as a reference over seven different sites of the US surface radiation budget observing network (SURFRAD) and five sites of the Australian OzFlux network.

Belen Franch

Quantitative Assessment of the HLS Surface Reflectance Consistency

The Harmonized Landsat and Sentinel-2 (HLS) project produces compatible surface reflectance (SR) from observations acquired by Landsat-8/9 OLI and Sentinel-2A/2B MSI. The HLS harmonization procedures include atmosphere correction, cloud masking, view angle normalization, and bandpass adjustment. The objective of this study is to quantitatively assess the reflectance consistency between Landsat and Sentinel-2 within the Version 2 HLS data. We collected 545 pairs of same-day Landsat/Sentinel-2 images across the globe to represent a wide range of vegetation types and climate regimes. The mean absolute difference (MAD) in reflectance between Landat and Sentinel-2 was calculated as a consistency indicator for each harmonization step. The MAD generally increased after the atmosphere correction, and then greatly decreased after the BRDF and bandpass adjustments, to smaller than the top-of-atmosphere MAD values. The MAD ranged from 0.0048 to 0.0093 for the six common spectral bands (blue, green, red, NIR, SWIR1, and SWIR2) in the final products, only slightly greater than the difference between Landsat and Sentinel-2 calibrations.. An evaluation on a few commonly used vegetation indices also showed good agreement between Landsat and Sentinel-2 reflectance. All these evaluations demonstrate that the HLS project produces a consistent SR dataset from Landsat-OLI and Sentinel-MSI, which will be a valuable resource for a wide range of remote sensing applications.

Qiang Zhou

Analysis of the Approach Direction of HLS to a South Pole Landing Site Subject to Lighting and Communication Constraints

The NRHO used as the staging orbit for crewed missions to the lunar surface by the HLS remains nearly perpendicular to the Earth-Moon line of sight, however, due to the libration of the Moon the location of the South Pole is ever shifting beneath it. DTE communication as well as ground illumination conditions near the South Pole coincide with lunar libration, with approximately 2-week cycles of DTE communication and solar availability. Additionally, the constant shifting of the South Pole region with respect to the NRHO leads to variations in the nominal HLS approach path to the surface in order to maintain near in-plane alignment of the NRHO, LLO, and approach phase. Making adjustments to the nominal approach path to achieve desired communication and lighting conditions quickly raises in-space ΔV. This analysis compares the ΔV impact of several trajectory strategies that could be used to alter the HLS approach path.

HLS

Horn Location Sensors (HLS) for LBNF

The Long-Baseline Neutrino Facility (LBNF) will deliver the world's most powerful muon neutrino beam to the Deep Underground Neutrino Experiment (DUNE), initially operating at 1.2 MW and upgradeable to 2.4 MW. Ensuring the accurate direction of this beam is critical for DUNE's precision goals. This talk introduces the Horn Location Sensors (HLS) system, designed to provide precise, relative measurements of the focusing horns, targets, and beam position monitors in the neutrino beamline. The HLS system employs high-precision FSI-based hydrostatic leveling sensors to track vertical motion and tilt, achieving precision on the order of 0.1 mm. Built for minimal maintenance in radioactive environments, the HLS system ensures precise alignment of beamline components during high-power operations. This system is essential for maintaining beam accuracy and enhancing DUNE's scientific performance.

43 PARTICLE ACCELERATORS

Human Landing System (HLS) Program Extravehicular Activity (EVA) Compatibility Interface Requirements Document (IRD)

The purpose of this document is to establish a set of EVA compatibility design requirements for the HLS Program. This document contains the fundamental information required for building hardware compatible with suited EVA flight crewmembers to perform EVAs. Compatibility requirements are located in section 3.0 with a gravity field applicability table provided in APPENDIX G, Human Landing System Program Applicability Matrix. Each HLS Provider will use the EHP-provided matrix in Appendix G to assess applicability to the awarded mission provider on a per-mission basis. Providers will negotiate applicability with EHP, and this will be documented in the appropriate Annex (reference section 2.2)

Christine N Kovich

HLS Mission Planning Using the STEDe Applications Suite of Timeline Evaluation and Development environments (STEDe)

STEDe applications work together to emulate the official HLS mission timeline and provide insight into potential mission constraints. They ingest HLS Program inputs such as ground rules and assumptions, requirements, and activity durations, informed by experts, to ensure that crew activities and critical vehicle events can transpire without conflicts and mission goals can be achieved. The STEDe applications provide timely assessments of what-if changes to the timeline with respect to different parameters and improve on the trial-and-error mission timeline development process.

Levi Gosdin

Human Landing System (HLS) Program Non-Design Driver Lunar Environments

The purpose of this white paper is to provide a source of information on lunar environments which, according to scientific and engineering consensus, should not impact human-class spacecraft design. Additionally, comparisons are shown between the Apollo missions and Artemis mission parameters for solar lighting angles at the landing sites and plasma region exposure.

HLS

Artemis III Sleep Scheduling with HYPNOS Mission Planning Tool HLS Yawn Prevention N Outer Space (HYPNOS)

HYPNOS ingests critical event data, such as burns and docking, and automatically shifts the crew’s sleep schedule to support these events where possible, while still being compliant with medical restrictions. HYPNOS analyzes the crew’s timeline in 3 states: 1) Awake, meaning the crew is active for work hours 2) Pre- and Post-Sleep, awake periods of limited activity as the crew gets ready to sleep or is waking up 3) Sleep, where the crew is inactive as they rest

Levi Gosdin