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Vibrationally excited ground state hydroxyl in fast flow system, considering mean radiative lifetime and reaction rate with ozone
Purification of enzyme responsible for degradation of nicotine to 6-hyroxynicotine
The lysine (Lys) hydroxylation pattern of type I collagen produced by human fibroblasts in culture was analyzed and compared. Fibroblasts were cultured from normal human skin (NSF), keloid (KDF), fetal skin (FDF), and skin tissues of Ehlers-Danlos syndrome type VIA and VIB patients (EDS-VIA and -VIB). The type I collagen alpha chains with or without non-helical telopeptides were purified from the insoluble matrix and analyzed. In comparison with NSFs, KDF and FDF showed significantly higher Lys hydroxylation, particularly in the telopeptide domains of both alpha chains. Both EDS-VIA and -VIB showed markedly lower Lys hydroxylation in the helical domains of both alpha chains whereas that in the telopeptides was comparable with those of NSFs. A similar profile was observed in the tissue sample of the EDS-VIB patient. These results demonstrate that the Lys hydroxylation pattern is domain-specific within the collagen molecule and that this method is useful to characterize the cell phenotypes in normal/pathological connective tissues.
The pattern of lysyl hydroxylation in the nontriple helical domains of collagen is critical in determining the cross-linking pathways that are tissue specific. We hypothesized that the tissue specificity of type I collagen cross-linking is, in part, due to the differential expression of lysyl hydroxylase genes (Procollagen-lysine,2-oxyglutarate,5-dioxygenase 1, 2, and 3 [PLOD1, PLOD2, and PLOD3]). In this study, we have examined the expression patterns of these three genes during the course of in vitro differentiation of human osteoprogenitor cells (bone marrow stromal cells [BMSCs]) and normal skin fibroblasts (NSFs). In addition, using the medium and cell layer/matrix fractions in these cultures, lysine hydroxylation of type I collagen alpha chains and collagen cross-linking chemistries have been characterized. High levels of PLOD1 and PLOD3 genes were expressed in both BMSCs and NSFs, and the expression levels did not change in the course of differentiation. In contrast to the PLOD1 and PLOD3 genes, both cell types showed low PLOD2 gene expression in undifferentiated and early differentiated conditions. However, fully differentiated BMSCs, but not NSFs, exhibited a significantly elevated level (6-fold increase) of PLOD2 mRNA. This increase coincided with the onset of matrix mineralization and with the increase in lysyl hydroxylation in the nontriple helical domains of alpha chains of type I collagen molecule. Furthermore, the collagen cross-links that are derived from the nontriple helical hydroxylysine-aldehyde were found only in fully differentiated BMSC cultures. The data suggests that PLOD2 expression is associated with lysine hydroxylation in the nontriple helical domains of collagen and, thus, could be partially responsible for the tissue-specific collagen cross-linking pattern.
An experiment is reported in which a balloon-borne lidar system was used to measure ozone and the hydroxyl radical in the stratosphere by two lidar techniques. Ozone was measured in the 20-37 km altitude range using differential absorption lidar, and the hydroxyl radical was measured in the 34-37 km range using remote laser-induced fluorescence. Ozone concentrations were determined with a vertical resolution of 0.5 km, and in addition, horizontally resolved ozone measurements with 0.15-km resolution were obtained over a 2-km range. The temporal variation of the hydroxyl radical concentration ranged from 40 parts/trillion shortly after noon to about 5 parts/trillion two hours after sunset. Possible modifications to the system are discussed which can yield an improvement in the sensitivity of between one and two orders of magnitude, thus permitting measurements of the hydroxyl radical in the 20-30-km altitude range.
Hydroxylation by solar wind protons has been simulated in our laboratory on Apollo 17 lunar sample 78421, a very mature regolith sample that is rich with agglutinates (68%). The goal of this study was to determine the rate of hydroxyl formation and their thermal stability by monitoring changes in the SiOH (hydroxyl) stretching band near 3 μm using diffuse reflectance FTIR spectroscopy (DRIFTS). A 2 keV H2+ ion beam was used to simulate proton implantation on 78421 and on a crushed fused silica sample. We find that the OH band does not change unless the samples have been annealed in vacuum prior to irradiation. Qualitatively, the OH bands for the fused silica and 78421 are very different. The OH band for fused silica is centered at 2.74 μm and is relatively sharp ranging from 2.67 - 3.1 μm at full-width-at-half-maximum (FWHM), while the OH band for 78421 is centered at 3.0 μm and ranges from 2.74 - 3.37 μm at FWHM. The increase in wavelength and broadened nature of the OH band in 78421 may be associated with the OH’s proximity to surface defects and/or lattice vacancies. The lack of the H2O bending mode at 6.1 μm indicates that any adsorbed terrestrial H2O is below our detection limit, and therefore the H2O stretching mode at 2.9 μm is not significantly contributing to the broad 3 μm OH band and implies that proton implantation by itself does not lead to water formation. To simulate the maximum dayside temperature on the lunar surface, the lunar sample was heated after proton irradiation. The proton induced OH concentration was reduced by as much as 25% after heating to 400 K (127 °C).
Production of hydroxyl containing curable liquid hydrocarbon prepolymers by ozonizing a high molecular weight saturated hydrocarbon polymer such as polyisobutylene or ethylene propylene rubber is discussed. The ozonized material is reduced using reducing agents, preferably diisobutyl aluminum hydride, to form the hydroxyl containing liquid prepolymers having a substantially lower molecular weight than the parent polymer. The resulting curable liquid hydroxyl containing prepolymers can be poured into a mold and readily cured, with reactants such as toluene diisocyanate, to produce highly stable elastomers having a variety of uses such as binders for solid propellants.
A balloon-borne laser radar (LIDAR) system for determining the stratospheric hydroxyl radical concentration constructed and flown by the Goddard Space Flight Center is discussed. The system measured hydroxyl in the altitude range of 34-37 km during an afternoon and early evening, producing the first post-sunset hydroxyl determination ever obtained. Daytime values (approximately 5 x 10 to the 6th/cu cm) are somewhat lower than previous measurements using in situ resonance fluorescence. Nighttime values (approximately 7 x 10 to the 5th/cu cm at 2100 CDT) are found to be higher than predicted by current one-dimensional models. Possible sources of these discrepancies, together with improvements possible in future systems, are outlined.
A combination of anthropogenic activities and a possible decline of global concentrations for the hydroxyl radicals that formerly removed methane from the atmosphere are cited as potential causes for the 1.3 percent/year rise of atmospheric methane levels. Calculations are presented which show that much of the methane increase over the last 200 years is probably to be divided among the two main sources in the proportions of 70 percent for anthropogenic generation and 30 percent for hydroxyl radical depletion. It is projected that in 20 years, average tropospheric concentrations of methane may be about 20 percent greater than 1980 levels. The current abundance of hydroxyl radicals may be 20 percent less than two centuries ago.
Knowledge of the concentration of hydroxyl radical is crucial to understanding the chemistry of the stratosphere. Hydroxyl participates in several catalytic cycles which destroy ozone and strongly influences the cycles of chlorine and nitrogen oxides by its reactions which form or destroy reservoirs for chlorine and odd-nitrogen compounds. Measurements have been conducted of the concentration of hydroxyl radical between the altitudes of 32.5 and 38.5 km using the technique of laser-induced fluorescence. The results ranging from 4 to 9 x 10 to the 6th per cu cm (with an accuracy of + or - 50 percent) are about 2-3 times lower than predicted by current one-dimensional models, although the uncertainties in the determination and in the models are sufficiently large to explain the differences. A number of potential improvements to the instrument are discussed.
A portion of an organic polymer article such as a membrane is made hydrophilic by exposing a hydrophobic surface of the article to a depth of about 50 to about 5000 angstroms to atomic oxygen or hydroxyl radicals at a temperature below 100C., preferably below 40 C, to form a hydrophilic uniform surface layer of hydrophilic hydroxyl groups. The atomic oxygen and hydroxyl radicals are generated by a flowing afterglow microwave discharge, and the surface is outside of a plasma produced by the discharge. A membrane having both hydrophilic and hydrophobic surfaces can be used in an immunoassay by adhering antibodies to the hydrophobic surface. In another embodiment, the membrane is used in cell culturing where cells adhere to the hydrophilic surface. Prior to adhering cells, the hydrophilic surface may be grafted with a compatibilizing compound. A plurality of hydrophilic regions bounded by adjacent hydrophobic regions can be produced such that a maximum of one cell per each hydrophilic region adheres.
Three-step thermal process quantitatively determines the hydroxyl content in samples of magnesium oxide. Analytical method can be adapted to large-scale production of hydroxyl-free magnesium oxide.
Reaction rate of vibrationally excited hydroxyl with ozone, obtaining hydroxyl emission spectra by Fourier transform spectroscopy
In the experiment discussed molecular oxygen was allowed to mix with atomic hydrogen and ozone at the entrance to a 3-m flow tube. A rate equation for the decay of the vibrationally excited hydroxyl is derived, taking into consideration the reaction with ozone, radiative decay, wall quenching, and interaction with molecular oxygen. It is pointed out that molecular oxygen might be important as a quencher for the hydroxyl airglow in the upper atmosphere in spite of negative results obtained in fast-flow experiments conducted in the laboratory.-
Profiles of static temperature and hydroxyl radical concentration were measured in a two-dimensional supersonic combustor test section 22.8 cm downstream of hydrogen injection. A high-pressure gas generator supplied vitiated air to the test section at Mach 2.44, atmospheric pressure, and a total temperature of about 2240 K. Room-temperature hydrogen was injected through a 0.40-cm step slot at Mach 1 and matched pressure. The measurements utilized a noninterfering spectral line absorption technique in which narrow ultraviolet emission lines of the hydroxyl electronic transition are absorbed by the broader absorption lines in the combustion gas. Comparison of the measured temperature profiles with theoretical calculations showed good agreement.
The behavior of the sodium and hydroxyl nighttime emissions during a stratospheric warming has been studied principally by use of data from the airglow photometers on the OGO-4 satellite. It was found that during the late stages of a major warming, both emissions increase appreciably, with the sodium emission returning to normal levels prior to the decrease in hydroxyl emission. The emission behaviors are attributed to temperature and density variations from 70 to 94 km, and a one-dimensional hydrostatic model for that altitude range is used to calculate the effects on the emissions and on the mesospheric ozone densities.
The behavior of the sodium and hydroxyl nighttime emissions during a stratospheric warming has been studied principally by use of data from the airglow photometers on the OGO 4 satellite. During the late stages of a major warming, both emissions increased appreciably, with the sodium emission returning to normal values prior to the decrease in the hydroxyl emission. The emission behaviors are attributed to temperature and density variations from 70 to 94 km, and a one-dimensional hydrostatic model for that altitude range is used to calculate the effects on the emissions and on the mesospheric ozone densities. These results support the existence of a warming in the upper part of the mesosphere that is correlated with a major stratospheric warming.
Comet Tago-Sato-Kosaka (1969 IX) was observed with the ultraviolet photometers on OAO 2 from January 16.41 to January 29.89, 1970, while its heliocentric distance increased from 0.78 to 1.03 AU. The production rates of hydrogen and hydroxyl are derived from Lyman-alpha (1216 A) and OH (0-0) band (3090 A) emission. The variations of the hydrogen and hydroxyl production ran parallel to one another, while their ratio was about 3:1. These results are consistent with the assumption that vaporization of water ice controlled the production rate of gas during this interval. The hydrogen production rates of four nonperiodic comets are compared.