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NASA NTRS2008

The Importance of the International Space Station for Life Sciences Research: Past and Future

The International Space Station (ISS) celebrates ten years of operations in 2008. While the station did not support permanent human crews during the first two years of operations, it hosted a few early science experiments months before the first international crew took up residence in November 2000. Since that time, science returns from the ISS have been growing at a steady pace. To date, early utilization of the U.S. Operating Segment of ISS has fielded nearly 200 experiments for hundreds of ground-based investigators supporting U.S. and international partner research. This paper will summarize the life science accomplishments of early research aboard the ISS both applied human research for exploration, and research on the effects of microgravity on life. At the 10-year point, the scientific returns from ISS should increase at a rapid pace. During the 2008 calendar year, the laboratory space and research facilities (both pressurized and external) will be tripled, with multiple scientific modules that support a wide variety of research racks and science and technology experiments conducted by all of the International Partners. A milestone was reached in February 2008 with the launch and commissioning of ESA s Columbus module and in March of 2008 with the first of three components of the Japanese Kibo laboratory. Although challenges lie ahead, the realization of the international scientific partnership provides new opportunities for scientific collaboration and broadens the research disciplines engaged on ISS. As the ISS nears completion of assembly in 2010, we come to full international utilization of the facilities for research. Using the past as an indicator, we are now able to envision the multidisciplinary contributions to improving life on Earth that the ISS can make as a platform for life sciences research.

Robinson, Julie A.↗

NASA NTRS2009

International Space Station Research and Facilities for Life Sciences

Assembly of the International Space Station is nearing completion in fall of 2010. Although assembly has been the primary objective of its first 11 years of operation, early science returns from the ISS have been growing at a steady pace. Laboratory facilities outfitting has increased dramatically 2008-2009 with the European Space Agency s Columbus and Japanese Aerospace Exploration Agency s Kibo scientific laboratories joining NASA s Destiny laboratory in orbit. In May 2009, the ISS Program met a major milestone with an increase in crew size from 3 to 6 crewmembers, thus greatly increasing the time available to perform on-orbit research. NASA will launch its remaining research facilities to occupy all 3 laboratories in fall 2009 and winter 2010. To date, early utilization of the US Operating Segment of the ISS has fielded nearly 200 experiments for hundreds of ground-based investigators supporting international and US partner research. With a specific focus on life sciences research, this paper will summarize the science accomplishments from early research aboard the ISS- both applied human research for exploration, and research on the effects of microgravity on life. We will also look ahead to the full capabilities for life sciences research when assembly of ISS is complete in 2010.

Robinson, Julie A.↗

NASA NTRS2000

Life Sciences Research in the Centrifuge Accommodation Module of the International Space Station

The Centrifuge Accommodation Module (CAM) will be the home of the fundamental biology research facilities on the International Space Station (ISS). These facilities are being built by the Biological Research Project (BRP), whose goal is to oversee development of a wide variety of habitats and host systems to support life sciences research on the ISS. The habitats and host systems are designed to provide life support for a variety of specimens including cells, bacteria, yeast, plants, fish, rodents, eggs (e.g., quail), and insects. Each habitat contains specimen chambers that allow for easy manipulation of specimens and alteration of sample numbers. All habitats are capable of sustaining life support for 90 days and have automated as well as full telescience capabilities for sending habitat parameters data to investigator homesite laboratories. The habitats provide all basic life support capabilities including temperature control, humidity monitoring and control, waste management, food, media and water delivery as well as adjustable lighting. All habitats will have either an internal centrifuge or are fitted to the 2.5-meter diameter centrifuge allowing for variable centrifugation up to 2 g. Specimen chambers are removable so that the specimens can be handled in the life sciences glovebox. Laboratory support equipment is provided for handling the specimens. This includes a compound and dissecting microscope with advanced video imaging, mass measuring devices, refrigerated centrifuge for processing biological samples, pH meter, fixation and complete cryogenic storage capabilities. The research capabilities provided by the fundamental biology facilities will allow for flexibility and efficiency for long term research on the International Space Station.

Dalton, Bonnie P.↗

NASA NTRS2021

SMD Technology Highlights

Six technology highlights from the Science Mission Directorate (SMD) Heliophysics, Astrophysics, Earth, and Biological and Physical Sciences divisions. Technologies featured include 1. the 1653 m2 Solar Cruiser Solar Sail Propulsion system to enable missions to reach novel and otherwise difficult or impossible destinations for observing the Sun. 2. Technology sponsored by NASA and developed by MIT Space Nanotechnology Lab is developing high-performance space instrumentation for more powerful future X-ray telescopes that will study the dynamics of the high-energy universe. 3. Active thermal control technology that will help enable large ultra-stable telescopes needed to detect and characterize Earth-like planets around other stars. 4. SMD sponsored high-performance infrared sensors with reduced requirements for cooling onboard satellites and these sensors could potentially be flown on small satellite platforms like CubeSats. 5. A new technology that will utilize electron beams/ultraviolet light to remove dust that could help protect future space assets and astronauts from dangerous effects that arise from Moon/Mars dust exposure. 6. Studies using model organisms on ISS to understand the impact of the spaceflight environment on organisms in preparation for long-duration missions.

solar sail↗

NASA NTRS2011

Life Science on the International Space Station Using the Next Generation of Cargo Vehicles

With the retirement of the Space Shuttle and the transition of the International Space Station (ISS) from assembly to full laboratory capabilities, the opportunity to perform life science research in space has increased dramatically, while the operational considerations associated with transportation of the experiments has changed dramatically. US researchers have allocations on the European Automated Transfer Vehicle (ATV) and Japanese H-II Transfer Vehicle (HTV). In addition, the International Space Station (ISS) Cargo Resupply Services (CRS) contract will provide consumables and payloads to and from the ISS via the unmanned SpaceX (offers launch and return capabilities) and Orbital (offers only launch capabilities) resupply vehicles. Early requirements drove the capabilities of the vehicle providers; however, many other engineering considerations affect the actual design and operations plans. To better enable the use of the International Space Station as a National Laboratory, ground and on-orbit facility development can augment the vehicle capabilities to better support needs for cell biology, animal research, and conditioned sample return. NASA Life scientists with experience launching research on the space shuttle can find the trades between the capabilities of the many different vehicles to be confusing. In this presentation we will summarize vehicle and associated ground processing capabilities as well as key concepts of operations for different types of life sciences research being launched in the cargo vehicles. We will provide the latest status of vehicle capabilities and support hardware and facilities development being made to enable the broadest implementation of life sciences research on the ISS.

Robinson, J. A.↗

NASA NTRS2008

NASA's Plans for Materials Science on ISS: Cooperative Utilization of the MSRR-MSL

The ISS Research Project draws Life (non-human) and Physical Sciences investigations on the ISS, free flyer and ground-based into one coordinated project. The project has two categories: I. Exploration Research Program: a) Utilizes the ISS as a low Technology Readiness Level (TRL) test bed for technology development, demonstration and problem resolution in the areas of life support, fire safety, power, propulsion, thermal management, materials technology, habitat design, etc.; b) Will include endorsement letters from other ETDP projects to show relevancy. II. Non-Exploration Research Program; a) Not directly related to supporting the human exploration program. Research conducted in the life (non-human) and physical sciences; b) The program will sustain, to the maximum extent practicable, the United States scientific expertise and research capability in fundamental microgravity research. Physical Sciences has about 44 grants, and Life Sciences has approximately 32 grants, mostly with universities, to conduct low TRL research; this includes grants to be awarded from the 2008 Fluid Physics and Life Science NRA's.

Chiaramonte, Francis↗

NASA NTRS2001

Space Station Biological Research Project Habitat: Incubator

Developed as part of the suite of Space Station Biological Research Project (SSBRP) hardware to support research aboard the International Space Station (ISS), the Incubator is a temperature-controlled chamber, for conducting life science research with small animal, plant and microbial specimens. The Incubator is designed for use only on the ISS and is transported to/from the ISS, unpowered and without specimens, in the Multi-Purpose Logistics Module (MPLM) of the Shuttle. The Incubator interfaces with the three SSBRP Host Systems; the Habitat Holding Racks (HHR), the Life Sciences Glovebox (LSG) and the 2.5 m Centrifuge Rotor (CR), providing investigators with the ability to conduct research in microgravity and at variable gravity levels of up to 2-g. The temperature within the Specimen Chamber can be controlled between 4 and 45 C. Cabin air is recirculated within the Specimen Chamber and can be exchanged with the ISS cabin at a rate of approximately equal 50 cc/min. The humidity of the Specimen Chamber is monitored. The Specimen Chamber has a usable volume of approximately equal 19 liters and contains two (2) connectors at 28v dc, (60W) for science equipment; 5 dedicated thermometers for science; ports to support analog and digital signals from experiment unique sensors or other equipment; an Ethernet port; and a video port. It is currently manifested for UF-3 and will be launched integrated within the first SSBRP Habitat Holding Rack.

Nakamura, G. J.↗

NASA NTRS2000

STS-97 ISS Science Payloads Briefing

John Un, International Space Station (ISS) Lead Increment Scientist, gives an overview of the STS-97 Endeavour mission payload (PV Module P6) and Expedition 1 crew. He describes the research and experimentation to take place on the ISS in the following fields: (1) Life Sciences, (2) Microgravity Research, (3) Commercial, (4) Space Sciences, and (5) Earth Sciences. Observations of Earth include images of the Aral Sea in central Asia and fires in Mongolia. Mr. Un then answers questions from the press.

Source record↗

NASA NTRS2016

Levitation Technology in International Space Station Research

The International Space Station (ISS) is a unique multidisciplinary orbiting laboratory for science and technology research, enabling discoveries that benefit life on Earth and exploration of the universe. ISS facilities for containerless sample processing in Materials Science experiments include levitation devices with specimen positioning control while reducing containment vessel contamination. For example, ESA's EML (ElectroMagnetic Levitator), is used for melting and solidification of conductive metals, alloys, or semiconductors in ultra-high vacuum, or in high-purity gaseous atmospheres. Sample heating and positioning are accomplished through electromagnetic fields generated by a coil system. EML applications cover investigation of solidification and microstructural formation, evaluation of thermophysical properties of highly reactive metals (whose properties can be very sensitive to contamination), and examination of undercooled liquid metals to understand metastable phase convection and influence convection on structural changes. MSL utilization includes development of novel light-weight, high-performance materials. Another facility, JAXA's ELF (Electrostatic Levitation Furnace), is used to perform high temperature melting while avoiding chemical reactions with crucibles by levitating a sample through Coulomb force. ELF is capable of measuring density, surface tension, and viscosity of samples at high temperatures. One of the initial ELF investigations, Interfacial Energy-1, is aimed at clarification of interfacial phenomena between molten steels and oxide melts with industrial applications in control processes for liquid mixing. In addition to these Materials Science facilities, other ISS investigations that involve levitation employ it for biological research. For example, NASA's "Magnetic 3D Culturing and Bioprinting" investigation uses magnetic levitation for three-dimensional culturing and positioning of magnetized cells to generate spheroid assemblies for biomedical applications. Levitation is also used as a modeled microgravity ground analog in the NASA OsteoOmics ISS investigation, which tests whether magnetic levitation accurately simulates microgravity conditions by studying gravitational regulation of osteoblast and osteoclast genomics and metabolism. Elucidating the cellular mechanisms of bone loss in microgravity contributes to the understanding of bone loss in medical disorders on Earth, which may lead to development of preventive or therapeutic countermeasures. Thus, the ISS state-of-the-art laboratory offers various levitation capability platforms with applications for innovative research in Materials and Life Sciences disciplines, with benefits for humanity.

Guinart-Ramirez, Y.↗

NASA NTRS2000

International Space Station Evolution Data Book: Baseline Design - Volume 1

The International Space Station (ISS) will provide an Earth-orbiting facility that will accommodate engineering experiments as well as research in a microgravity environment for life and natural sciences. The ISS will distribute resource utilities and support permanent human habitation for conducting this research and experimentation in a safe and habitable environment. The objectives of the ISS program are to develop a world-class, international orbiting laboratory for conducting high-value scientific research for the benefit of humans on Earth; to provide access to the microgravity environment; to develop the ability to live and work in space for extended periods; and to provide a research test bed for developing advanced technology for human and robotic exploration of space. The current design and development of the ISS has been achieved through the outstanding efforts of many talented engineers, designers, technicians, and support personnel who have dedicated their time and hard work to producing a state-of-the-art Space Station. Despite these efforts, the current design of the ISS has limitations that have resulted from cost and technology issues. Regardless, the ISS must evolve during its operational lifetime to respond to changing user needs and long-term national and international goals. As technologies develop and user needs change, the ISS will be modified to meet these demands. The design and development of these modifications should begin now to prevent a significant lapse in time between the baseline design and the realization of future opportunities. For this effort to begin, an understanding of the baseline systems and current available opportunities for utilization needs to be achieved. Volume I of this document provides the consolidated overview of the ISS baseline systems. It also provides information on the current facilities available for pressurized and unpressurized payloads. Information on current plans for crew availability and utilization; resource timelines and margin summaries including power, thermal, and storage volumes; and an overview of the ISS cargo traffic and the vehicle traffic model is also included.

Jorgensen, Catherine A.↗

NASA NTRS2009

Humans on the International Space Station-How Research, Operations, and International Collaboration are Leading to New Understanding of Human Physiology and Performance in Microgravity

As the International Space Station (ISS) nears completion, and full international utilization is achieved, we are at a scientific crossroads. ISS is the premier location for research aimed at understanding the effects of microgravity on the human body. For applications to future human exploration, it is key for validation, quantification, and mitigation of a wide variety of spaceflight risks to health and human performance. Understanding and mitigating these risks is the focus of NASA s Human Research Program. However, NASA s approach to defining human research objectives is only one of many approaches within the ISS international partnership (including Roscosmos, the European Space Agency, the Canadian Space Agency, and the Japan Aerospace Exploration Agency). Each of these agencies selects and implements their own ISS research, with independent but related objectives for human and life sciences research. Because the science itself is also international and collaborative, investigations that are led by one ISS partner also often include cooperative scientists from around the world. The operation of the ISS generates significant additional data that is not directly linked to specific investigations. Such data comes from medical monitoring of crew members, life support and radiation monitoring, and from the systems that have been implemented to protect the health of the crew (such as exercise hardware). We provide examples of these international synergies in human research on ISS and highlight key early accomplishments that derive from these broad interfaces. Taken as a whole, the combination of diverse research objectives, operational data, international sharing of research resources on ISS, and scientific collaboration provide a robust research approach and capability that no one partner could achieve alone.

Ronbinson, Julie A.↗

NASA NTRS2015

Spaceflight Causes Increased Virulence of Serratia Marcescens on a Drosophila Melanogaster Host

Drosophila melanogaster, or the fruit fly, has long been an important organism for Earth-based research, and is now increasingly utilized as a model system to understand the biological effects of spaceflight. Studies in Drosophila melanogaster have shown altered immune responses in 3rd instar larvae and adult males following spaceflight, changes similar to those observed in astronauts. In addition, spaceflight has also been shown to affect bacterial physiology, as evidenced by studies describing altered virulence of Salmonella typhimurium following spaceflight and variation in biofilm growth patterns for the opportunistic pathogen Pseudomonas aeruginosa during flight. We recently sent Serratia marcescens Db11, a Drosophila pathogen and an opportunistic human pathogen, to the ISS on SpaceX-5 (Fruit Fly Lab-01). S. marcescens samples were stored at 4degC for 24 days on-orbit and then allowed to grow for 120 hours at ambient station temperature before being returned to Earth. Upon return, bacteria were isolated and preserved in 50% glycerol or RNAlater. Storage, growth, and isolation for ground control samples were performed using the same procedures. Spaceflight and ground samples stored in 50% glycerol were diluted and injected into 5-7-day-old ground-born adult D. melanogaster. Lethality was significantly greater in flies injected with the spaceflight samples compared to those injected with ground bacterial samples. These results indicate a shift in the virulence profile of the spaceflight S. marcescens Db11 and will be further assessed with molecular biological analyses. Our findings strengthen the conclusion that spaceflight impacts the virulence of bacterial pathogens on model host organisms such as the fruit fly. This research was supported by NASA's ISS Program Office (ISSPO) and Space Life and Physical Sciences Research and Applications (SLPSRA).

Serratia marcescens↗

NASA NTRS2007

Research on the International Space Station: Understanding Future Potential from Current Accomplishments

In November 2007, the International Space Station (ISS) will have supported seven years of continuous presence in space, with 15 Expeditions completed. These years have been characterized by the numerous technical challenges of assembly as well as operational and logistical challenges related to the availability of transportation by the Space Shuttle. During this period, an active set of early research objectives have also been accomplished alongside the assembly. This paper will review the research accomplishments on ISS to date, with the objective of drawing insights on the potential of future research following completion of ISS assembly. By the end of Expedition 15, an expected 121 U.S.-managed investigations will have been conducted on ISS, with 91 of these completed. Many of these investigations include multiple scientific objectives, with an estimated total of 334 scientists served. Through February 2007, 101 scientific publications have been identified. Another 184 investigations have been sponsored by ISS international partners, which independently track their scientists served and results publication. Through this survey of U.S. research completed on ISS, three different themes will be addressed: (1) How have constraints on transportation of mass to orbit affected the types of research successfully completed on the ISS to date? What lessons can be learned for increasing the success of ISS as a research platform during the period following the retirement of the Space Shuttle? (2) How have constraints on crew time for research during assembly and the active participation of crewmembers as scientists affected the types of research successfully completed on the ISS to date? What lessons can be learned for optimizing research return following the increase in capacity from 3 to 6 crewmembers (planned for 2009)? What lessons can be learned for optimizing research return after assembly is complete? (3) What do early research results indicate about the various scientific disciplines represented in investigations on ISS? Are there lessons specific to human research, technology development, life sciences, and physical sciences that can be used to increase future research accomplishments? Research has been conducted and completed on ISS under a set of challenging constraints during the past 7 years. The history of research accomplished on ISS during this time serves as an indicator of the value and potential of ISS when full utilization begins. By learning from our early experience in completing research on ISS, NASA and our partners can be positioned to optimize research returns as a full crew complement comes onboard, assembly is completed, and research begins in full.

Robinson, Julie A.↗

NASA NTRS2017

NASA Ames Institutional Scientific Collection (ISC)

NASA's current human space flight research is directed towards enabling human space exploration beyond Low Earth Orbit (LEO). The Space Flight Payload Projects; Rodent Research, Cell Science, and Microbial Labs, flown on the International Space Station (ISS), benefit both the global life sciences and commercial space communities. Verified data sets, science results, peer-reviewed publications, and returned biospecimens, collected and analyzed for flight and ground investigations, are all part of the knowledge base within NASA’s Human Exploration and Operations Mission Directorate’s Space Life and Physical Sciences Research and Applications (SLPSRA) Division, specifically the Human Research and Space Biology Programs. These data and biospecimens are made available through the public LSDA website. The Ames Institutional Scientific Collection (ISC), or ARC Biobank, stores flight and ground biospecimens from Space Shuttle and ISS programs. These specimens are curated and managed by the Ames Life Sciences Data Archive (ALSDA), an internal node of NASA's Life Sciences Data Archive (LSDA). The ARC Biolbank stores over 15,000 specimens from experiments dating from 1984 to present. Currently available specimens include tissues from the circulatory, digestive, endocrine, excretory, integumentary, muscular, neurosensory, reproductive, respiratory and skeletal systems. The most recent contributions include RNA, DNA and protein extracts from Rodent Research 1 and tissues from Rodent Research 4. NASA's biospecimen collection represents a unique and limited resource. The use of these biospecimens maximizes utilization and scientific return from these unique spaceflight payload and ground control research subjects. These biospecimens are harvested following complex, costly NASA research activities to meet primary scientific objectives. Once the primary scientific objectives have been met, the remaining specimens are made available to provide secondary opportunities for complementary studies or new investigations to broaden research without large expenditures of time or resources. Innovative ways of sharing this information ultimately advances the frontiers of human space exploration as well as scientific understanding of the effects of gravity on life on earth.

French, Alison J.↗

NASA NTRS2020

Accelerating Space Life Sciences: Successes and Challenges of Biospecimen and Data Sharing

NASA's current human space flight research is directed towards enabling human space exploration beyond Low Earth Orbit (LEO). To that end, NASA Space Flight Payload Projects; Rodent Research, Cell Science, and Microbial Labs, flown on the International Space Station (ISS), benefit the global life sciences and commercial space communities. Verified data sets, science results, peer-reviewed publications, and returned biospecimens, collected and analyzed for flight and ground investigations, are all part of the knowledge base collected by NASA's Human Exploration and Operations Mission Directorate's Space Life and Physical Sciences Research and Applications (SLPSRA) Division, specifically the Human Research and Space Biology Programs. These data and biospecimens are made available through the public Life Sciences Data Archive (LSDA) website to promote basic discovery, pre-clinical and clinical science.The NASA Institutional Scientific Collection (ISC), stores flight and ground biospecimens from Space Shuttle and ISS programs. These specimens are curated and managed by the Ames Life Sciences Data Archive (ALSDA), an internal node of NASA's LSDA. The ISC stores over 30,000 specimens from experiments dating from 1984 to present. Currently available specimens include tissues from the circulatory, digestive, endocrine, excretory, integumentary, muscular, neurosensory, reproductive, respiratory and skeletal systems.NASA's biospecimen collection represents a unique and limited resource of unique spaceflight payload and ground control research subjects. These specimens are harvested according to well established SOPs that maintain their quality and integrity. Once the primary scientific objectives have been met, the remaining specimens are made available to provide secondary opportunities for complementary studies or new investigations to broaden research without large expenditures of time or resources. Website: https://lsda.jsc.nasa.gov/

Scott, Ryan T.↗

NASA NTRS2016

Rodent Habitat On ISS: Spaceflight Effects On Mouse Behavior

The NASA Decadal Survey (2011), Recapturing a Future for Space Exploration: Life and Physical Sciences Research for a New Era, emphasized the importance of expanding NASA life sciences research to long duration, rodent experiments on the International Space Station (ISS). To accomplish this objective, flight hardware, operations, and science capabilities supporting mouse studies in space were developed at NASA Ames Research Center. The first flight experiment carrying mice, Rodent Research Hardware and Operations Validation (Rodent Research-1), was launched on Sept 21, 2014 in an unmanned Dragon Capsule, SpaceX4, exposing the mice to a total of 37 days in space. Ground control groups were maintained in environmental chambers at Kennedy Space Center. Mouse health and behavior were monitored for the duration of the experiment via video streaming. Here we present behavioral analysis of two groups of five C57BL/6 female adult mice viewed via fixed camera views compared with identically housed Ground Controls. Flight (Flt) and Ground Control (GC) mice exhibited the same range of behaviors, including eating, drinking, exploratory behavior, self- and allo-grooming, and social interactions at similar or greater levels of occurrence. Mice propelled themselves freely and actively throughout the Habitat using their forelimbs to push off or by floating from one cage area to another, and they quickly learned to anchor themselves using tails and/or paws. Overall activity was greater in Flt as compared to GC mice, with spontaneous ambulatory behavior including the development of organized ‘circling’ or ‘race-tracking’ behavior that emerged within the first few days of flight and encompassed the primary dark cycle activity for the remainder of the experiment. We quantified the bout frequency, duration and rate of circling with respect to characteristic behaviors observed in the varying stages of the progressive development of circling: flipping utilizing two sides of the habitat, circling, multi-lap circling and group-circling. Once begun, mice did not regress to flipping behavior or other previous behavioral milestones for the remainder of flight. An overall upward trend in circling frequency, rate, duration, participation, and organization was observed over the course of the 37-day spaceflight experiment. In this presentation, we will summarize qualitative observations and quantitative comparisons of mice in microgravity and 1g conditions. Behavioral analyses provide important insights into the overall health and adaptation of mice to the space environment, and identify unique behaviors and social interactions to guide future habitat development and research on rodents in space.

Mouse↗

NASA NTRS2016

Overview of the Life Science Glovebox (LSG) Facility and the Research Performed in the LSG

The Life Science Glovebox (LSG) is a rack facility currently under development with a projected availability for International Space Station (ISS) utilization in the FY2018 timeframe. Development of the LSG is being managed by the Marshal Space Flight Center (MSFC) with support from Ames Research Center (ARC) and Johnson Space Center (JSC). The MSFC will continue management of LSG operations, payload integration, and sustaining following delivery to the ISS. The LSG will accommodate life science and technology investigations in a "workbench" type environment. The facility has a.Ii enclosed working volume that is held at a negative pressure with respect to the crew living area. This allows the facility to provide two levels of containment for handling Biohazard Level II and lower biological materials. This containment approach protects the crew from possible hazardous operations that take place inside the LSG work volume. Research investigations operating inside the LSG are provided approximately 15 cubic feet of enclosed work space, 350 watts of28Vdc and l IOVac power (combined), video and data recording, and real time downlink. These capabilities will make the LSG a highly utilized facility on ISS. The LSG will be used for biological studies including rodent research and cell biology. The LSG facility is operated by the Payloads Operations Integration Center at MSFC. Payloads may also operate remotely from different telescience centers located in the United States and different countries. The Investigative Payload Integration Manager (IPIM) is the focal to assist organizations that have payloads operating in the LSG facility. NASA provides an LSG qualification unit for payload developers to verify that their hardware is operating properly before actual operation on the ISS. This poster will provide an overview of the LSG facility and a synopsis of the research that will be accomplished in the LSG. The authors would like to acknowledge Ames Research Center, Johnson Space Center, Teledyne Brown Engineering, MOOG-Bradford Engineering and the entire LSG Team for their inputs into this abstract.

Cole, J. Michael↗

NASA NTRS2016

Rodent Habitat on ISS: Advances in Capability for Determining Spaceflight Effects on Mammalian Physiology

Rodent research is a valuable essential tool for advancing biomedical discoveries in life sciences on Earth and in space. The National Research Counsel's Decadal survey (1) emphasized the importance of expanding NASAs life sciences research to perform long duration, rodent experiments on the International Space Station (ISS). To accomplish this objective, new flight hardware, operations, and science capabilities were developed at NASA ARC to support commercial and government-sponsored research. The flight phases of two separate spaceflight missions (Rodent Research-1 and Rodent Research-2) have been completed and new capabilities are in development. The first flight experiments carrying 20 mice were launched on Sept 21, 2014 in an unmanned Dragon Capsule, SpaceX4; Rodent Research-1 was dedicated to achieving both NASA validation and CASIS science objectives, while Rodent Reesearch-2 extended the period on orbit to 60 days. Groundbased control groups (housed in flight hardware or standard cages) were maintained in environmental chambers at Kennedy Space Center. Crewmembers previously trained in animal handling transferred mice from the Transporter into Habitats under simultaneous veterinary supervision by video streaming and were deemed healthy. Health and behavior of all mice on the ISS was monitored by video feed on a daily basis, and post-flight quantitative analyses of behavior were performed. The 10 mice from RR-1 Validation (16wk old, female C57Bl6/J) ambulated freely and actively throughout the Habitat, relying heavily on their forelimbs for locomotion. The first on-orbit dissections of mice were performed successfully, and high quality RNA (RIN values>9) and liver enzyme activities were obtained, validating the quality of sample recovery. Post-flight sample analysis revealed that body weights of FLT animals did not differ from ground controls (GC) housed in the same hardware, or vivarium controls (VIV) housed in standard cages. Organ weights analyzed post-flight showed that there were no differences between FLT and GC groups in adrenal gland and spleen weights, whereas FLT thymus and liver weights exceeded those of GC. Minimal differences between the control groups (GC and VIV) were observed. In addition, Over 3,000 aliquots collected post-flight from the four groups of mice were deposited into the Ames Life Science Data Archives for the Biospecimen Sharing Program and Genelab project. New capabilities recently developed include DEXA scanning, grip strength tests and male mice. In conclusion, new capability for long duration rodent habitation of group-housed rodents was developed and includes in-flight sample collection, thus avoiding the complication of reentry. Results obtained to date reveal the possibility of striking differences between the effects of short duration vs. long duration spaceflight. This Rodent Research system enables achievement of both basic science and translational research objectives to advance human exploration of space.

Bone Loss↗