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Development and application of operational techniques for the inventory and monitoring of resources and uses for the Texas coastal zone

The author has identified the following significant results. Four LANDSAT scenes were analyzed for the Harbor Island area test sites to produce land cover and land use maps using both image interpretation and computer-assisted techniques. When evaluated against aerial photography, the mean accuracy for three scenes was 84% for the image interpretation product and 62% for the computer-assisted classification maps. Analysis of the fourth scene was not completed using the image interpretation technique, because of poor quality, false color composite, but was available from the computer technique. Preliminary results indicate that these LANDSAT products can be applied to a variety of planning and management activities in the Texas coastal zone.

Harwood, P.

Composite x-ray image assembly for large-field digital mammography with one- and two-dimensional positioning of a focal plane array

To demonstrate the feasibility of a novel large-field digital mammography technique, a 1024 x 1024 pixel Loral charge-coupled device (CCD) focal plane array (FPA) was positioned in a mammographic field with one- and two-dimensional scan sequences to obtain 950 x 1800 pixel and 3600 x 3600 pixel composite images, respectively. These experiments verify that precise positioning of FPAs produced seamless composites and that the CCD mosaic concept has potential for high-resolution, large-field imaging. The proposed CCD mosaic concept resembles a checkerboard pattern with spacing left between the CCDs for the driver and readout electronics. To obtain a complete x-ray image, the mosaic must be repositioned four times, with an x-ray exposure at each position. To reduce the patient dose, a lead shield with appropriately patterned holes is placed between the x-ray source and the patient. The high-precision motorized translation stages and the fiber-coupled-scintillating-screen-CCD sensor assembly were placed in the position usually occupied by the film cassette. Because of the high mechanical precision, seamless composites were constructed from the subimages. This paper discusses the positioning, image alignment procedure, and composite image results. The paper only addresses the formation of a seamless composite image from subimages and will not consider the effects of the lead shield, multiple CCDs, or the speed of motion.

Mammography/methods/statistics & numerical data

Three-dimensional characterization of tethered microspheres by total internal reflection fluorescence microscopy

Tethered particle microscopy is a powerful tool to study the dynamics of DNA molecules and DNA-protein complexes in single-molecule experiments. We demonstrate that stroboscopic total internal reflection microscopy can be used to characterize the three-dimensional spatiotemporal motion of DNA-tethered particles. By calculating characteristic measures such as symmetry and time constants of the motion, well-formed tethers can be distinguished from defective ones for which the motion is dominated by aberrant surface effects. This improves the reliability of measurements on tether dynamics. For instance, in observations of protein-mediated DNA looping, loop formation is distinguished from adsorption and other nonspecific events.

Non-NASA Center

Development and application of operational techniques for the inventory and monitoring of resources and uses for the Texas coastal zone. Volume 1: Text

The author has identified the following significant results. Image interpretation and computer-assisted techniques were developed to analyze LANDSAT scenes in support of resource inventory and monitoring requirements for the Texas coastal region. Land cover and land use maps, at a scale of 1:125,000 for the image interpretation product and 1:24,000 for the computer-assisted product, were generated covering four Texas coastal test sites. Classification schemes which parallel national systems were developed for each procedure, including 23 classes for image interpretation technique and 13 classes for the computer-assisted technique. Results indicate that LANDSAT-derived land cover and land use maps can be successfully applied to a variety of planning and management activities on the Texas coast. Computer-derived land/water maps can be used with tide gage data to assess shoreline boundaries for management purposes.

Harwood, P.

Use of multiresolution wavelet feature pyramids for automatic registration of multisensor imagery

The problem of image registration, or the alignment of two or more images representing the same scene or object, has to be addressed in various disciplines that employ digital imaging. In the area of remote sensing, just like in medical imaging or computer vision, it is necessary to design robust, fast, and widely applicable algorithms that would allow automatic registration of images generated by various imaging platforms at the same or different times and that would provide subpixel accuracy. One of the main issues that needs to be addressed when developing a registration algorithm is what type of information should be extracted from the images being registered, to be used in the search for the geometric transformation that best aligns them. The main objective of this paper is to evaluate several wavelet pyramids that may be used both for invariant feature extraction and for representing images at multiple spatial resolutions to accelerate registration. We find that the bandpass wavelets obtained from the steerable pyramid due to Simoncelli performs best in terms of accuracy and consistency, while the low-pass wavelets obtained from the same pyramid give the best results in terms of the radius of convergence. Based on these findings, we propose a modification of a gradient-based registration algorithm that has recently been developed for medical data. We test the modified algorithm on several sets of real and synthetic satellite imagery.

Evaluation Studies

Image processing for improved eye-tracking accuracy

Video cameras provide a simple, noninvasive method for monitoring a subject's eye movements. An important concept is that of the resolution of the system, which is the smallest eye movement that can be reliably detected. While hardware systems are available that estimate direction of gaze in real-time from a video image of the pupil, such systems must limit image processing to attain real-time performance and are limited to a resolution of about 10 arc minutes. Two ways to improve resolution are discussed. The first is to improve the image processing algorithms that are used to derive an estimate. Off-line analysis of the data can improve resolution by at least one order of magnitude for images of the pupil. A second avenue by which to improve resolution is to increase the optical gain of the imaging setup (i.e., the amount of image motion produced by a given eye rotation). Ophthalmoscopic imaging of retinal blood vessels provides increased optical gain and improved immunity to small head movements but requires a highly sensitive camera. The large number of images involved in a typical experiment imposes great demands on the storage, handling, and processing of data. A major bottleneck had been the real-time digitization and storage of large amounts of video imagery, but recent developments in video compression hardware have made this problem tractable at a reasonable cost. Images of both the retina and the pupil can be analyzed successfully using a basic toolbox of image-processing routines (filtering, correlation, thresholding, etc.), which are, for the most part, well suited to implementation on vectorizing supercomputers.

NASA Discipline Space Human Factors

Digital storage and analysis of color Doppler echocardiograms

Color Doppler flow mapping has played an important role in clinical echocardiography. Most of the clinical work, however, has been primarily qualitative. Although qualitative information is very valuable, there is considerable quantitative information stored within the velocity map that has not been extensively exploited so far. Recently, many researchers have shown interest in using the encoded velocities to address the clinical problems such as quantification of valvular regurgitation, calculation of cardiac output, and characterization of ventricular filling. In this article, we review some basic physics and engineering aspects of color Doppler echocardiography, as well as drawbacks of trying to retrieve velocities from video tape data. Digital storage, which plays a critical role in performing quantitative analysis, is discussed in some detail with special attention to velocity encoding in DICOM 3.0 (medical image storage standard) and the use of digital compression. Lossy compression can considerably reduce file size with minimal loss of information (mostly redundant); this is critical for digital storage because of the enormous amount of data generated (a 10 minute study could require 18 Gigabytes of storage capacity). Lossy JPEG compression and its impact on quantitative analysis has been studied, showing that images compressed at 27:1 using the JPEG algorithm compares favorably with directly digitized video images, the current goldstandard. Some potential applications of these velocities in analyzing the proximal convergence zones, mitral inflow, and some areas of future development are also discussed in the article.

NASA Discipline Cardiopulmonary

X-ray digital imaging petrography of lunar mare soils: modal analyses of minerals and glasses

It is essential that accurate modal (i.e., volume) percentages of the various mineral and glass phases in lunar soils be used for addressing and resolving the effects of space weathering upon reflectance spectra, as well as for their calibration such data are also required for evaluating the resource potential of lunar minerals for use at a lunar base. However, these data are largely lacking. Particle-counting information for lunar soils, originally obtained to study formational processes, does not provide these necessary data, including the percentages of minerals locked in multi-phase lithic fragments and fused-soil particles, such as agglutinates. We have developed a technique for modal analyses, sensu stricto, of lunar soils, using digital imaging of X-ray maps obtained with an energy-dispersive spectrometer mounted on an electron microprobe. A suite of nine soils (90 to 150 micrometers size fraction) from the Apollo 11, 12, 15, and 17 mare sites was used for this study. This is the first collection of such modal data on soils from all Apollo mare sites. The abundances of free-mineral fragments in the mare soils are greater for immature and submature soils than for mature soils, largely because of the formation of agglutinitic glass as maturity progresses. In considerations of resource utilization at a lunar base, the best lunar soils to use for mineral beneficiation (i.e., most free-mineral fragments) have maturities near the immature/submature boundary (Is/FeO approximately or = 30), not the mature soils with their complications due to extensive agglutination. The particle data obtained from the nine mare soils confirm the generalizations for lunar soils predicted by L.A. Taylor and D.S. McKay (1992, Lunar Planet Sci. Conf. 23rd, pp. 1411-1412 [Abstract]).

NASA Discipline Exobiology

Automated quantification of mitral valve regurgitation based on normalized centerline velocity distribution

Previous echocardiographic techniques for quantifying valvular regurgitation are limited by factors including uncertainties for orifice location and a hemispheric convergence assumption that often results in over- and underestimation of flow rate and regurgitant orifice area. Using computational fluid dynamics simulations, these factors were eliminated, allowing a more accurate assessment of regurgitation. A model was developed to allow automated quantification of regurgitant orifice diameter based on the centerline velocity data available from color M-mode echocardiography. The model, validated using in vitro unsteady flow data, demonstrated improved accuracy for orifice diameter (y=0.95x + 0.38, r=0.96) and volume (y=1.18x - 4.72, r=0.93).

NASA Program Biomedical Research and Countermeasur

Coastal pollution hazards in southern California observed by SAR imagery: stormwater plumes, wastewater plumes, and natural hydrocarbon seeps

Stormwater runoff plumes, municipal wastewater plumes, and natural hydrocarbon seeps are important pollution hazards for the heavily populated Southern California Bight (SCB). Due to their small size, dynamic and episodic nature, these hazards are difficult to sample adequately using traditional in situ oceanographic methods. Complex coastal circulation and persistent cloud cover can further complicate detection and monitoring of these hazards. We use imagery from space-borne synthetic aperture radar (SAR), complemented by field measurements, to examine these hazards in the SCB. The hazards are detectable in SAR imagery because they deposit surfactants on the sea surface, smoothing capillary and small gravity waves to produce areas of reduced backscatter compared with the surrounding ocean. We suggest that high-resolution SAR, which obtains useful data regardless of darkness or cloud cover, could be an important observational tool for assessment and monitoring of coastal marine pollution hazards in the SCB and other urbanized coastal regions.

Water Movements

Implementation of ILLIAC 4 algorithms for multispectral image interpretation

Research has focused on the design and partial implementation of a comprehensive ILLIAC software system for computer-assisted interpretation of multispectral earth resources data such as that now collected by the Earth Resources Technology Satellite. Research suggests generally that the ILLIAC 4 should be as much as two orders of magnitude more cost effective than serial processing computers for digital interpretation of ERTS imagery via multivariate statistical classification techniques. The potential of the ARPA Network as a mechanism for interfacing geographically-dispersed users to an ILLIAC 4 image processing facility is discussed.

Ray, R. M.

Advanced feature extraction in remote sensing using artificial intelligence and geographic information systems

Traditional computer-assisted image-analysis techniques in remote sensing lag well behind human abilities in terms of both speed and accuracy. A fundamental limitation of computer-assisted techniques is their inability to assimilate a variety of different data types leading to an interpretation in a manner similar to human image interpretation. Expert systems and computer-vision techniques are proposed as a potential solution to these limitations. Some aspects of human expertise in image analysis may be codified into expert systems. Image understanding and symbolic reasoning provide a means of assimilating spatial information and spatial reasoning into the analysis procedure. Knowledge-based image-analysis systems incorporate many of these concepts and have been implemented for some well defined problem domains. Geographic information systems represent an excellent environment for this type of analysis, providing both analytic tools and contextual information to the analysis procedure.

Estes, John E.

Characterization of LANDSAT-4 TM and MSS Image Quality for the Interpretation of California's Agricultural Resources

The quality of LANDSAT-4 MSS and TM data was determined by analyzing TM spectral and spatial performance in terms of spectral variability of natural targets and the TM-ground instantaneous field-of-view (IFOV) variability in level and mountainous terrain; and by assessing the suitability of TM and MSS image products for characterizing renewable resourse features. The TM data should be extremelly valuable for crop type and area proportion estimation; undating agricultural land use survey maps at 1:24,000 scale and smaller, field boundary definition; and determining the size and location of individual farmsteads. Ongoing research activities are focused on making spectral and spatial analyses of both MSS and TM analytical film products. The improved spectral, spatial, and radiometric quality of the TM data, should promote a renewed emphasis and interest in direct visual interpretation of these image products, both for updating and improving land stratification in support of resource inventory and for enhancing the image analyst's contribution to computer-assisted analysis procedures.

Degloria, S. D.

Compression of echocardiographic scan line data using wavelet packet transform

An efficient compression strategy is indispensable for digital echocardiography. Previous work has suggested improved results utilizing wavelet transforms in the compression of 2D echocardiographic images. Set partitioning in hierarchical trees (SPIHT) was modified to compress echocardiographic scanline data based on the wavelet packet transform. A compression ratio of at least 94:1 resulted in preserved image quality.

Non-NASA Center

Color M-mode Doppler flow propagation velocity is a preload insensitive index of left ventricular relaxation: animal and human validation

OBJECTIVES: To determine the effect of preload in color M-mode Doppler flow propagation velocity (v(p)). BACKGROUND: The interpretation of Doppler filling patterns is limited by confounding effects of left ventricular (LV) relaxation and preload. Color M-mode v(p) has been proposed as a new index of LV relaxation. METHODS: We studied four dogs before and during inferior caval (IVC) occlusion at five different inotropic stages and 14 patients before and during partial cardiopulmonary bypass. Left ventricular (LV) end-diastolic volumes (LV-EDV), the time constant of isovolumic relaxation (tau), left atrial (LA) pre-A and LV end-diastolic pressures (LV-EDP) were measured. Peak velocity during early filling (E) and v(p) were extracted by digital analysis of color M-mode Doppler images. RESULTS: In both animals and humans, LV-EDV and LV-EDP decreased significantly from baseline to IVC occlusion (both p < 0.001). Peak early filling (E) velocity decreased in animals from 56 +/- 21 to 42 +/- 17 cm/s (p < 0.001) without change in v(p) (from 35 +/- 15 to 35 +/- 16, p = 0.99). Results were similar in humans (from 69 +/- 15 to 53 +/- 22 cm/s, p < 0.001, and 37 +/- 12 to 34 +/- 16, p = 0.30). In both species, there was a strong correlation between LV relaxation (tau) and v(p) (r = 0.78, p < 0.001, r = 0.86, p < 0.001). CONCLUSIONS: Our results indicate that color M-mode Doppler v(p) is not affected by preload alterations and confirms that LV relaxation is its main physiologic determinant in both animals during varying lusitropic conditions and in humans with heart disease.

Non-NASA Center