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Ectomycorrhizal Community Structure and Soil Characteristics of Mature Lodgepole Pine (Pinus Contorta) and Adjacent Stands of Old Growth Mixed Conifer in Yellowstone National Park, Wyoming USA

Forest development patterns following disturbance are known to influence the physical and chemical attributes of soils at different points in time. Changes in soil resources are thought to have a corresponding effect on ectomycorrhizal (ECM) community structure. We used molecular methods to compare below-ground ECM species richness, composition, and abundance between adjacent stands of homogenous lodgepole pine and old growth mixed conifer in Yellowstone National Park (YNP). In each stand-type we collected soil cores to both identify mycorrhizae and assess soil chemistry. Although no statistical difference was observed in the mean number of ECM root tips per core between stand types, the total number of species identified (85 versus 35) and the mean number of species per core (8.8 +/- 0.6 versus 2.5 +/- 0.3) were significantly higher in lodgepole pine. Differences between the actual and estimated species richness levels indicated that these forest types support a high number of ECM species and that undersampling was severe. Species compositions were widely disparate between stands where only four species were shared out of a total of 116. Soil analysis also revealed that mixed conifer was significantly lower in pH, but higher in organic matter, potassium, phosphorus, and ammonium when compared to lodgepole pine stands. Species richness per core was correlated with these chemical data, however, analysis of covariance indicated that stand type was the only statistically significant factor in the observed difference in species richness. Our data suggest that ECM fungal richness increases as homogenous lodgepole pine stands grow and mature, but declines after Engelmann spruce and subalpine fir colonize. Despite difficulties linking species composition with soil chemistry, there are a variety of physical and chemical factors that could be influencing ECM community structure. Future field experiments are necessary to test some of the mechanisms potentially operating within this system.

Douglas, Robert B.↗

Oleoresin, Chemistry and Spectral Reflectance in "Stressed" Lodgepole and White Bark Pine, Mammoth Mountain, California

Development of methods to identify the physical and chemical character of materials on the earth's surface is one of the foci of hyperspectral remote sensing activities. Enhancing the ability to elucidate changes in foliar chemistry that relate to the health of a plant is a benefit to plant physiologists, foresters, and plant ecologists, as well as geologist and environmental scientists. Vegetation covers the landscape throughout the temperate and tropical regions of the earth. The existence of vegetation in these areas presents special problems to remote sensing systems since geologic bedrock and alteration zones are masked. At times, however, alterations in the soil/sediment geochemical environment result in foliar chemical changes that are detectable via remote sensing. Examples include monitoring of chlorophyll reflectance/fluorescence and equivalent water thickness indices as indicators of drought-induced plant stress. Another processing and interpretation approach used with hyperspectral data has been principal components analysis (PCA). Rowan et al. used PCA to identify absorption feature patterns obtained from vegetated areas with discrete bedrock geology or mineralization as the substrate. Many researchers highlight the need to advance our ability for hyperspectral imaging in vegetated areas as a near-term priority.

Hickey, James C.↗

Effects of litter addition on ectomycorrhizal associates of a lodgepole pine (Pinus contorta) stand in Yellowstone National Park

Increasing soil nutrients through litter manipulation, pollution, or fertilization can adversely affect ectomycorrhizal (EM) communities by inhibiting fungal growth. In this study, we used molecular genetic methods to determine the effects of litter addition on the EM community of a Pinus contorta stand in Yellowstone National Park that regenerated after a stand-replacing fire. Two controls were used; in unmodified control plots nothing was added to the soil, and in perlite plots perlite, a chemically neutral substance, was added to maintain soil moisture and temperature at levels similar to those under litter. We found that (i) species richness did not change significantly following perlite addition (2.6 +/- 0.3 species/core in control plots, compared with 2.3 +/- 0.3 species/core in perlite plots) but decreased significantly (P < 0.05) following litter addition (1.8 +/- 0.3 species/core); (ii) EM infection was not affected by the addition of perlite but increased significantly (P < 0.001) in response to litter addition, and the increase occurred only in the upper soil layer, directly adjacent to the added litter; and (iii) Suillus granulatus, Wilcoxina mikolae, and agaricoid DD were the dominant organisms in controls, but the levels of W. mikolae and agaricoid DD decreased significantly in response to both perlite and litter addition. The relative levels of S. granulatus and a fourth fungus, Cortinariaceae species 2, increased significantly (P < 0.01 and P < 0.05, respectively) following litter addition. Thus, litter addition resulted in some negative effects that may be attributable to moisture-temperature relationships rather than to the increased nutrients associated with litter. Some species respond positively to litter addition, indicating that there are differences in their physiologies. Hence, changes in the EM community induced by litter accumulation also may affect ecosystem function.

Fungi/classification/growth & development↗

Investigation of the detection and monitoring of forest insect infestations in the Sierra Nevada Mountains of California

The author has identified the following significant results. In earlier reports it has been indicated that it is possible to delineate areas of lodgepole pine timber mortality into three degrees of damage from enlarged ERTS-1 color composites; light, medium, and heavy. It can also be confidently reported that it is now possible to detect all major areas of lodgepole pine defoliated by the needle miner. It has also been confirmed, through ground checking and helicopter observation that previous designation of the following features have been consistently accurate: timbered vs non-timbered areas; timber types; damaged vs undamaged areas; lakes, dome shadows which resemble lakes, mountain meadows, pasture and agricultural land, desert; riparian vegetation; and glaciers.

Hall, R. C.↗

Investigation of the detection and monitoring of forest insect infestations in the Sierra Nevada Mountains of California

The author has identified the following significant results. It is possible to detect all major areas of lodgepole pine defoliated by the needle miner within a given target area. Ground checking and helicopter observations have confirmed that accurate designations have been obtained for the following areas: (1) timbered v.s. non-timbered areas, (2) damaged v.s. undamaged timber areas, (3) lakes, (4) dome shadows which resemble lakes, (5) mountain meadows, (6) pasture land, (7) agricultural land, (8) desert, and (9) riparian vegetation.

Hall, R. C.↗

Inventory of forest and rangeland and detection of forest stress

The author has identified the following significant results. Controlled visual interpretation of one ERTS-1 scene taken at the peak of the growing season has indicated that classification to the ECOCLASS Series level is not entirely satisfactory. For five forest classes, aspen, Douglas-fir, lodgepole pine, ponderosa pine, and Spruce/fir, correct identification ranged from 60 to 70 percent. With the exception of shortgrass and wet shrubby meadow classes in the nonforest categories (81 and 100 percent correct, respectively), correct identification of the nonforest classes is so far unacceptable. The low accuracies are believed due to: (1) edge effects due to ecotones between plant community classes with apparent similar image characteristics; (2) confounding effects of amount of plant crown cover and ground surface material in the scene; and (3) variable land slope degree and aspect as it affects the image signature.

Heller, R. C.↗

Geology and forestry classification from ERTS-1 digital data

Computer classifications into seven and ten classes of two areas in central Oregon of interest to geology and forestry demonstrate the extraction of information from ERTS-1 data. The area around Newberry Caldera was classified into basalt, rhyolite obsidian, pumice flats, Newberry pumice, ponderosa pine, lodgepole pine, and water classes. The area around Mt. Washington was classified into two basalts, three forest, two clearcut, burn, snow, and water classes. Both also include an unclassified category. Significant details that cannot be extracted from photographic reconstitutions of the data emerge from these classifications, such as moraine locations and paleowind directions. Spectral signatures for the various rocks are comparable to those published elsewhere.

Lawrence, R. D.↗

Interpreting vegetation reflectance measurements as a function of solar zenith angle

Spectral hemispherical-conical reflectances of a nadir looking sensor were taken throughout the day for a lodgepole pine and two grass canopies. Mathematical simulations of both spectral hemispherical-conical and bi-hemispherical reflectances were performed for two theoretical canopies of contrasting geometric structure. These results and comparisons with literature studies showed a great amount of variability of vegetation canopy reflectances as a function of solar zenith angle. Explanations for this variability are discussed and recommendations for further measurements are proposed.

Kimes, D. S.↗

Simulation of solar radiation absorption in vegetation canopies

A solar radiation canopy absorption model, including multiple scattering effects, was developed and tested for a lodgepole pine (Pinus contorta) canopy. Reflectance above the canopy, spectral transmittance to the ground layer, and geometric and spectral measurements of canopy elements were made. Relatively large differentials occurred in spectral absorption by canopy layers, especially in the photosynthetically active region, as a function of solar zenith angle. In addition, the proportion of total global irradiance absorbed by individual layers varied greatly as a function of solar zenith angle. However, absorption by the entire canopy system remained relatively constant.

Kimes, D. S.↗

Vegetation reflectance measurements as a function of solar zenith angle

An understanding of the behavior of vegetation canopy reflectance as a function of solar zenith angle is important to several remote sensing applications. Spectral hemispherical-conical reflectances of a nadir looking sensor were taken throughout the day of a lodgepole pine and two grass canopies. Mathematical simulations of both a spectral hemispherical-conical reflectance factor and a spectral bi-hemispherical reflectance were performed for two theoretical canopies of contrasting geometric structure. These results and results from literature studies showed a great amount of variability of vegetation canopy reflectances as a function of solar zenith angle. Explanations for this variability are discussed and recommendations for future measurements are proposed.

Kimes, D. S.↗

Thermal IR exitance model of a plant canopy

A thermal IR exitance model of a plant canopy based on a mathematical abstraction of three horizontal layers of vegetation was developed. Canopy geometry within each layer is quantitatively described by the foliage and branch orientation distributions and number density. Given this geometric information for each layer and the driving meteorological variables, a system of energy budget equations was determined and solved for average layer temperatures. These estimated layer temperatures, together with the angular distributions of radiating elements, were used to calculate the emitted thermal IR radiation as a function of view angle above the canopy. The model was applied to a lodgepole pine (Pinus contorta) canopy over a diurnal cycle. Simulated vs measured radiometric average temperatures of the midcanopy layer corresponded with 2 C. Simulation results suggested that canopy geometry can significantly influence the effective radiant temperature recorded at varying sensor view angles.

Kimes, D. S.↗

Calibration and evaluation of AVIRIS data: Cripple Creek in October 1987

Airborne Visible/Infrared Imaging Spectrometer (AVIRIS) data were obtained over Cripple Creek and Canon City Colorado on October 19, 1987 at local noon. Multiple ground calibration sites were measured within both areas with a field spectrometer and samples were returned to the laboratory for more detailed spectral characterization. The data were used to calibrate the AVIRIS data to ground reflectance. Once calibrated, selected spectra in the image were extracted and examined, and the signal to noise performance was computed. Images of band depth selected to be diagnostic of the presence of certain minerals and vegetation were computed. The AVIRIS data were extremely noisy, but images showing the presence of goethite, kaolinite and lodgepole pine trees agree with ground checks of the area.

Clark, Roger N.↗

Response of vegetation indices to changes in three measures of leaf water stress

The responses of vegetation indices to changes in water stress were evaluated in two separate laboratory experiments. In one experiment the normalized difference vegetation index (NDVI), the near-IR to red ratio (near-IR/red), the Infrared Index (II), and the Moisture Stress Index (MSI) were more highly correlated to leaf water potential in lodgepole pine branches than were the Leaf Water Content Index (LWCI), the mid-IR ratio (Mid-IR), or any of the single Thematic Mapper (TM) bands. In the other experiment, these six indices and the TM Tasseled Cap brightness, greenness, and wetness indices responded to changes in leaf relative water content (RWC) differently than they responded to changes in leaf water content (WC) of three plant species, and the responses were dependent on how experimental replicates were pooled. With no pooling, the LWCI was the most highly correlated index to both RWC and WC among replications, followed by the II, MSI, and wetness. Only the LWCI was highly correlated to RWC and WC when replications were pooled within species. With among species pooling the LWCI was the only index highly correlated with RWC, while the II, MSI, Mid-IR, and wetness were most highly correlated with WC.

Cohen, Warren B.↗

Surveying Dead Trees and CO2-Induced Stressed Trees Using AVIRIS in the Long Valley Caldera

Since 1980 the Long Valley Caldera in the eastern Sierra Nevada (California) has shown signs of renewed volcanic activity. Frequent earthquakes, a re-inflation of the caldera, hydrothermal activity and gas emissions are the outer symptoms of this renewed activity. In 1990 and 1991 several areas of dying trees were found around Mammoth Mountain. The cause of the die off of the trees was first sought in the persistent drought in the preceding years. However, the trees died regardless of age and species. Farrar et al. (1995) started a soil-gas survey in 1994 in the dead-tree areas and found carbon dioxide concentrations ranging from 30 to 96% at soil depths between 30 and 60 cm. CO2 concentrations in the atmosphere are usually around 0.03% and in the soil profile CO2 levels do commonly not exceed 4 to 5%. Although not much is known about the effect of high levels of carbon dioxide in the soil profile on roots, it is most likely that the trees are dying due to oxygen deprivation: the CO2 drives the oxygen out of the soil. So far, four sites of dead trees have been mapped around Mammoth Mountain. The two largest dying trees sites are located near Horseshoe Lake and near Mammoth Mountain Main Lodge covering approximately an area of 10 and 8 ha respectively. Analysis of the gas composition regarding the He-3/He-4 ratio and the percentage biogenic carbon reveals the source of the gas: the magma body beneath the Long Valley Caldera. Until recently it was not known that volcanoes release abundant carbon dioxide from their flanks as diffuse soil emanations. As a result of the magma gas emission around Mammoth Mountain there is an excellent sequence of dead trees, stressed trees, healthy trees and bare soil surfaces. This research site provides excellent opportunities to: (1) Study the capabilities of imaging spectrometry to map stressed (and dead) pine and fir species; (2) Study methods to separate the vivid vegetation, stressed vegetation and dead vegetation from the soil background of glacial deposits and crystalline rocks. The dead tree areas are located on the flanks of Mammoth Mountain (N:37 deg 37' 45" and W:119 deg 02' 05") at an elevation between 2600 and 3000 meters. The area is covered by an open type of Montane Forest. The dominant tree species are Lodgepole Pine (Pinus contorta), the Red Fir (Abies magnifica) and the Jeffrey Pine (Pinus jeffreyi). The soil surface near Horseshoe Lake is generally fairly bright. The surface is covered by glacial deposits (till) consisting mainly of weathered granitic rocks.

deJong, Steven M.↗