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Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

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At least 19 records
All sourcesNASA NTRSDOE OSTI
DOE OSTI2024

Genomic profiles of four novel cyanobacteria MAGs from Lake Vanda, Antarctica: insights into photosynthesis, cold tolerance, and the circadian clock

Cyanobacteria in polar environments face environmental challenges, including cold temperatures and extreme light seasonality with small diurnal variation, which has implications for polar circadian clocks. However, polar cyanobacteria remain underrepresented in available genomic data, and there are limited opportunities to study their genetic adaptations to these challenges. This paper presents four new Antarctic cyanobacteria metagenome-assembled genomes (MAGs) from microbial mats in Lake Vanda in the McMurdo Dry Valleys in Antarctica. The four MAGs were classified as Leptolyngbya sp. BulkMat.35, Pseudanabaenaceae cyanobacterium MP8IB2.15, Microcoleus sp. MP8IB2.171, and Leptolyngbyaceae cyanobacterium MP9P1.79. The MAGs contain 2.76 Mbp – 6.07 Mbp, and the bin completion ranges from 74.2–92.57%. Furthermore, the four cyanobacteria MAGs have average nucleotide identities (ANIs) under 90% with each other and under 77% with six existing polar cyanobacteria MAGs and genomes. This suggests that they are novel cyanobacteria and demonstrates that polar cyanobacteria genomes are underrepresented in reference databases and there is continued need for genome sequencing of polar cyanobacteria. Analyses of the four novel and six existing polar cyanobacteria MAGs and genomes demonstrate they have genes coding for various cold tolerance mechanisms and most standard circadian rhythm genes with the Leptolyngbya sp. BulkMat.35 and Leptolyngbyaceae cyanobacterium MP9P1.79 contained kaiB3, a divergent homolog of kaiB.

59 BASIC BIOLOGICAL SCIENCES↗
NASA NTRS2015

Solar Probe Plus MAG Sensor Thermal Design for Low Heater Power and Extreme Thermal Environment

The heater power available for the Solar Probe Plus FIELDS MAG sensor is less than half of the heritage value for other missions. Nominally the MAG sensors are in the spacecraft's umbra. In the worst hot case, approximately 200 spacecraft communication downlinks, up to 10 hours each, are required at 0.7 AU. These downlinks require the spacecraft to slew 45 deg. about the Y-axis, exposing the MAG sensors and boom to sunlight. This paper presents the thermal design to meet the MAG sensor thermal requirements in the extreme thermal environment and with low heater power. A thermal balance test on the MAG sensor engineering model has verified the thermal design and correlated the thermal model for flight temperature predictions.

Thermal design↗
DOE OSTI2024

Depth-specific distribution of bacterial MAGs in permafrost active layer in Ny Ålesund, Svalbard (79°N)

Arctic soil microbial communities may shift with increasing temperatures and water availability from climate change. We examined temperature and volumetric liquid water content (VWC) in the upper 80 cm of permafrost-affected soil over 2 years (2018–2019) at the Bayelva monitoring station, Ny Ålesund, Svalbard. We show VWC increases with depth, whereas in situ temperature is more stable vertically, ranging from -5°C to 5°C seasonally. Prokaryotic metagenome-assembled genomes (MAGs) were obtained at 2–4 cm vertical resolution collected while frozen in April 2018 and at 10 cm vertical resolution collected while thawed in September 2019. The most abundant MAGs were Acidobacteriota, Actinomycetota, and Chloroflexota. Actinomycetota and Chloroflexota increase with depth, while Acidobacteriota classes Thermoanaerobaculia Gp7-AA8, Blastocatellia UBA7656, and Vicinamibacteria Vicinamibacterales are found above 6 cm, below 6 cm, and below 20cm, respectively. All MAGs have diverse carbon-degrading genes, and Actinomycetota and Chloroflexota have autotrophic genes. Further, genes encoding β -glucosidase, N-acetyl-β-D-glucosaminidase, and xylosidase increase with depth, indicating a greater potential for organic matter degradation with higher VWC. Acidobacteriota dominate the top 6 cm with their classes segregating by depth, whereas Actinomycetota and Chloroflexota dominate below ~6 cm. This suggests that Acidobacteriota classes adapt to lower VWC at the surface, while Actinomycetota and Chloroflexota persist below 6cm with higher VWC. This indicates that VWC may be as important as temperature in microbial climate change responses in Arctic mineral soils. Here we describe MAG-based Seqcode type species in the Acidobacteriota, Onstottus arcticum, Onstottus frigus, and Gilichinskyi gelida and in the Actinobacteriota, Mayfieldus profundus.

54 ENVIRONMENTAL SCIENCES↗
DOE OSTI2026

Data Mining of Groundwater to Identify MAGs with Methane, Propane and Toluene Monooxygenases

Whole genome sequencing datasets, involving more than 600 groundwater samples, from nine countries, were analyzed to identify metagenome assembled genomes (MAGs) containing full operons for propane monooxygenase, soluble methane monooxygease, toluene monooxygenase and particulate ammonia/methane monooxygenase. The enzymes encoded by these genes are a focus of interest because of their ability to degrade common groundwater contaminants. Due to the large amount of data, sequence analyses involved more than 80 individual KBase narratives. The approach followed the KBase tutorial called "Metagenome-Assembled Genome Extraction from a Compost Microbiome Enrichment" The generated MAGs were exported from each individual narrative into separate summary KBase narratives for each monooxygenase. Three KBase narratives were generated for particulate ammonia/methane monooxygenase, due to the large number of MAGs identified.

59 BASIC BIOLOGICAL SCIENCES↗
NASA NTRS1991

RW Per - Nodal motion changes its amplitude by 1.4 mag

RW Per was found to have large secular changes in its eclipse amplitude. In blue light, for example, the amplitude was 3.2 mag in the early 1900s, 2.2 mag in the late 1960s, and 1.75 mag in 1990. Throughout this time, the brightness at maximum was constant in all colors. It is shown that the only possible explanation is nodal motion, where the inclination varies with a period of roughly 100,000 yr. The nodal motion is caused by a third star, for which the light curve, the colors, and the O - C curve already provide evidence. Thus, RW Per is only the fourth known star with large changes of eclipse amplitude and is only the second example of nodal motion.

Schaefer, Bradley E.↗
NASA NTRS1988

US 943 - A 19th mag eclipsing cataclysmic variable with a period of 2 hr 3.8 min

The high-latitude, V equals about 19.5, blue variable star US 943 is found to be an eclipsing binary with an orbital period of 2.06 hr. The light curve is dominated by an orbital hump which has an amplitude of 0.9 mag in V and is centered about 0.17 orbital cycles before the 1.5-mag deep eclipse. There is evidence that the eclipse has at least two components. The overall appearance of the star is that of a dwarf nova in quiescence, a conclusion that is supported by the observation of a subsequent bright state (V equals about 15) suggesting a dwarf nova outburst.

Howell, S. B.↗
NASA NTRS2017

AZ-2000-IECW and StaMet Black Kapton Options for Solar Probe Plus MAG Sensor MLI Kevlar/Polyimide Shells

AZ-2000-IECW white paint and StaMet black Kapton have been evaluated for the Kevlar/polyimide shells that enclose the Solar Probe Plus Magnetometer (MAG) sensors and multilayer insulation. Flight qualification testing on AZ-2000-IECW painted Kevlar/polyimide laminate was completed at Goddard Space Flight Center. This paint potentially meets all the requirements. However, it has no flight heritage. StaMet is hotter in the sun, and is specular. The results of the MAG thermal balance test show StaMet meets the thermal requirement and heater power budget. The mission prefers to fly StaMet after evaluating the risks of AZ-2000-IECW flaking and glint from StaMet to the Star Trackers.

AZ-2000-IECW; StaMet Black Kapton; Kevlar/Polyimid↗
DOE OSTI2026

Post Wildfire Soil Bacterial MAGs and Metagenome Analysis

We compare the differences between bacteria in soil affected by a wildfire to an unaffected area from Minnewaska State Park, NY located in the biodiverse northern Shawangunk ridge. We detail our metagenomic sequencing data, relative abundance of bacterial phyla, and the taxonomic classification of three high-quality MAGs.

59 BASIC BIOLOGICAL SCIENCES↗
DOE OSTI2022

PNV J00444033+4113068: Early superhumps with 0.7 mag amplitude and non-red color

Abstract In the first days of WZ Sge-type dwarf nova (DN) outbursts, the 2 : 1 resonance induces a spiral arm structure in the accretion disk, which is observed as early superhumps in optical light curves. We reports on our optical observations of an eclipsing WZ Sge-type DN PNV J00444033+4113068 during its 2021 superoutburst using the 3.8 m Seimei telescope and through the Variable Star Network collaboration. The eclipse analysis showed that its orbital period was 0.055425534(1) d. Our observations confirmed early superhumps with an amplitude of 0.7 mag, the largest amplitude among known WZ Sge-type DNe. More interestingly, its early superhumps became the reddest around their secondary minimum, whereas other WZ Sge-type DNe show the reddest color around the early superhump maximum. The spectrum around the peak of the outburst showed two double-peaked emission lines of He ii 4686 Å and Hα with a peak separation of ≥700 km s−1, supporting a very high-inclination system. With the early superhump mapping, the unique profile and color of the early superhump are successfully reproduced by an accretion disk with a vertically extended double arm structure. Therefore, a large amplitude and a unique color behavior of the early superhumps in PNV J00444033+4113068 can be explained by the 2 : 1 resonance model along with other WZ Sge-type DNe.

Tampo, Yusuke (ORCID:0000000301963936)↗
DOE OSTI2023

Lawrence Livermore National Laboratory Experimental Test Site 300 (Site 300): Site 300 Roadway Improvements - Chem Mag Loop Soil Sampling and Analysis Plan (May 2023)

This Soil Sampling and Analysis Plan (SAP) was prepared by the Environmental Function Area (EFA)/Technical Services Department (TSD) of the Environment, Safety & Health (ES&H) Directorate for the Project Management Office (PMO) for the proposed Roadway Improvements Project at the Chem Mag Loop (project). The purpose of the SAP is to describe the procedures for collection and analysis of environmental samples and evaluation of analytical data (chemical and radiological) to determine management options of excavated soil during project construction. This SAP follows criteria established in Lawrence Livermore National Laboratory’s (LLNL) Soils Screening and Management Plan (SSMP) (LLNL 2022), which was formalized in accordance with U.S. Environmental Protection Agency (EPA) guidance for developing Data Quality Objectives for environmental data (EPA 2006) and the Multi-Agency Radiation Survey and Site Investigation Manual (MARSSIM) guidance (U.S. NRC, U.S. EPA, U.S. DOE, and U.S. DOD 2000). The scope of this SAP is based on preliminary design information provided to EFA by PMO.

54 ENVIRONMENTAL SCIENCES↗
DOE OSTI2025

MAGs from alcoholic fermentation in sugarcane biorefineries

Genome-resolved metagenomics was applied to recover microbial metagenome-assembled genomes (MAGs) from alcoholic fermentation samples collected at two sugarcane biorefineries in São Paulo state, Brazil, during the 2024 harvest season.

59 BASIC BIOLOGICAL SCIENCES↗
NASA NTRS1996

Feedback Linearization in a Six Degree-of-Freedom MAG-LEV Stage

A six degree-of-freedom electromagnetically suspended motion control stage (the Angstrom Stage) has been designed and constructed for use in short-travel, high-resolution motion control applications. It achieves better than 0.5 nm resolution over a 100 micron range of travel. The stage consists of a single moving element (the platen) floating in an oil filled chamber. The oil is crucial to the stage's operation since it forms squeeze film dampers between the platen and the frame. Twelve electromagnetic actuators provide the forces necessary to suspend and servo the platen, and six capacitance probes measure its position relative to the frame. The system is controlled using a digital signal processing board residing in a '486 based PC. This digital controller implements a feedback linearization algorithm in real-time to account for nonlinearities in both the magnetic actuators and the fluid film dampers. The feedback linearization technique reduces a highly nonlinear plant with coupling between the degrees of freedom into one that is linear, decoupled, and setpoint independent. The key to this procedure is a detailed plant model. The operation of the feedback linearization procedure is transparent to the outer loop of the controller, and so a proportional controller is sufficient for normal operation. We envision applications of this stage in scanned probe microscopy and for integrated circuit measurement.

Ludwick, Stephen J.↗