Membrane Chaperoning of a Thylakoid Protease Whose Structural Stability Is Modified by the Protonmotive Force
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Biochemistry & Molecular Biology↗
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Process for manufacturing shell membrane force and deflection sensor
A sensor for force is formed from an elastomeric cylinder having a region with apertures. The apertures have passageways formed between them, and an optical fiber is introduced into these passageways, where the optical fiber has a grating for measurement of tension positioned in the passageways between apertures. Optionally, a temperature measurement sensor is placed in or around the elastomer for temperature correction, and if required, a copper film may be deposited in the elastomer for reduced sensitivity to spot temperature variations in the elastomer near the sensors.
Park, Yong-Lae↗
Electrochromic shift supports the membrane destabilization model of Tat-mediated transport and shows ion leakage during Sec transport
Significance Due to the transient nature of the active Tat complex, much of the knowledge available has been hard won. Electric-field-indicating absorbance transients allow investigation of transmembrane ion movement using native electric potential in thylakoids while circumventing challenges of reconstitution. Electrical behavior of transiently and/or constitutively assembled complexes suggests that Sec translocation occurs through a barrel-stave-type proteinaceous pore, while Tat translocation likely occurs through a toroidal pore. This would be the first instance of toroidal pores being exploited by a general protein translocation system. Our electrochemical analysis offers insight into how protein transport might be linked to light stimulation. Localized membrane destabilization presents a rational link between the proton motive force, membrane thinning, and Tat-mediated transport of substrates critical to photosynthesis.
59 BASIC BIOLOGICAL SCIENCES↗
On the solution of conical shells of linearly varying thickness subjected to lateral normal loads.
Asymptotic and particular solutions of conical shells subjected to lateral normal loads, discussing membrane forces, bending effects and boundary conditions for moments and shearing force
SHEARING STRESS↗
Deflections of anisotropic sandwich beams with variable face sheets and core thicknesses
A sandwich construction consists of a low-density core material with high strength face sheets bounded to the top and bottom surfaces. The construction has been widely used in the aerospace and marine industries due to its outstanding characteristics such as noise absorption, weight minimization, heat insulation, and better bending stiffness. In sandwich structures used in high-performance aircraft, the face sheets are often made of fiber-reinforced composite materials and the core is made of honeycomb. The structures may also have variable thickness so as to satisfy aerodynamic requirements. In the stress analysis, the constant-thickness face sheets are usually considered as membrane and the core is assumed to be inextensible but deformable in the thickness direction. The static behavior of variable-thickness, isotropic and homogeneous sandwich beams was successfully studied by employing a constant-thickness theory but allowing stiffnesses to vary in accordance with local thickness variations. It has been recently found in a refined theory that the analyses based on the constant thickness theory locally can lead to significant errors in structural responses if the sandwich beam is thickness-tapered and the cores are deformable in transverse shear. The errors arise mainly from two factors: (1) the transverse shear components of the membrane forces in the face sheets alter the transverse shears carried by the core; and (2) the face-sheet membrane strains arise from transverse shear deformation of the core. In practice the variable thickness may not only exist in core but also in face sheets. The thickness-variations may even be a type of step function. In this case the transverse shear stress in the face sheets and bending stress in the core should be taken into account in the refined theory mentioned. In the present study, energy principles are employed in deriving governing equations for general bending of anisotropic sandwich beams with variable thickness in both face sheets and cores. Solutions to these equations are based on a finite difference scheme. As an example in application, a simply supported thickness-tapered sandwich beam subject to a concentrated load at its center is considered. Let W' be the maximum deflection of the beam in which face sheets are considered as membrane, while W'' is that based on using the modified refined theory. It is found that W' is always larger than W'', however, the magnitude of (W'- W'') appears to be insensitive to the change of the taper of the beam.
Lu, Chu-Ho↗
Computer program performs rectangular fitting stress analysis
Computer program simulates specific bulkhead fittings by subjecting the desired geometry configuration to a membrane force, an external force, an external moment, an external tank pressure, or any combination of the above. This program generates a general model of bulkhead fittings for the Saturn booster.
Bertrand, A. R.↗
Transverse shear effect in a circumferentially cracked cylindrical shell
The objectives of the paper are to solve the problem of a circumferentially-cracked cylindrical shell by taking into account the effect of transverse shear, and to obtain the stress intensity factors for the bending moment as well as the membrane force as the external load. The formulation of the problem is given for a specially orthotropic material within the framework of a linearized shallow shell theory. The particular theory used permits the consideration of all five boundary conditions as to moment and stress resultants on the crack surface. The effect of Poisson's ratio on the stress intensity factors and the nature of the out-of-plane displacement along the edges of the crack, i.e., bulging, are also studied.
Delale, F.↗
The effect of low velocity impact in the strength characteristics of composite materials laminates
The nonlinear vibration response of a double cantilevered beam subjected to pulse loading over a central sector is studied. The initial response is generated in detail to ascertain the energetics of the response. The total energy is used as a gauge of the stability and accuracy of the solution. It is shown that to obtain accurate and stable initial solutions an extremely high spatial and time resolution is required. This requirement was only evident through an examination of the energy of the system. It is proposed, therefore, to use the total energy of the system as a necessary stability and accuracy criterion for the nonlinear response of conservative systems. The results also demonstrate that even for moderate nonlinearities, the effects of membrane forces have a significant influence on the system.
Liebowitz, H.↗
Energy conservation in the transient response of nonlinear beam vibration problems subjected to pulse loading - A numerical approach
The nonlinear vibration response of a double cantilevered beam subjected to pulse loading over a central sector is studied. The initial response is generated in detail to ascertain the energetics of the response. The total energy is used as a gauge of the stability and accuracy of the solution. It is shown that to obtain accurate and stable initial solutions an extremely high spatial and time resolution is required. This requirement was only evident through an examination of the energy of the system. It is proposed, therefore, to use the total energy of the system as a necessary stability and accuracy criterion for the nonlinear response of conservative systems. The results also demonstrate that even for moderate nonlinearities, the effects of membrane forces have a significant influence on the system. It is also shown that while the fundamental response is contained in a first mode envelope, the fluctuations caused by the higher order modes must be resolved.
Moyer, E. T., Jr.↗
Multiplexed Force and Deflection Sensing Shell Membranes for Robotic Manipulators
Force sensing is an essential requirement for dexterous robot manipulation, e.g., for extravehicular robots making vehicle repairs. Although strain gauges have been widely used, a new sensing approach is desirable for applications that require greater robustness, design flexibility including a high degree of multiplexibility, and immunity to electromagnetic noise. This invention is a force and deflection sensor a flexible shell formed with an elastomer having passageways formed by apertures in the shell, with an optical fiber having one or more Bragg gratings positioned in the passageways for the measurement of force and deflection.
Park, Yong-Lae↗
The force exerted by the membrane potential during protein import into the mitochondrial matrix
The force exerted on a targeting sequence by the electrical potential across the inner mitochondrial membrane is calculated on the basis of continuum electrostatics. The force is found to vary from 3.0 pN to 2.2 pN (per unit elementary charge) as the radius of the inner membrane pore (assumed aqueous) is varied from 6.5 to 12 A, its measured range. In the present model, the decrease in force with increasing pore width arises from the shielding effect of water. Since the pore is not very much wider than the distance between water molecules, the full shielding effect of water may not be present; the extreme case of a purely membranous pore without water gives a force of 3.2 pN per unit charge, which should represent an upper limit. When applied to mitochondrial import experiments on the protein barnase, these results imply that forces between 11 +/- 2 pN and 13.5 +/- 2.5 pN catalyze the unfolding of barnase in those experiments. A comparison of these results with unfolding forces measured using atomic force microscopy is made.
Models, Molecular↗
The Force Exerted by the Membrane Potential During Protein Import into the Mitochondrial Matrix
The electrostatic force exerted on a targeting sequence by the electrical potential across the inner mitochondrial membrane is calculated and found to vary from 1.4 pN to 2.2 pN (per unit elementary charge) as the radius of the inner membrane pore (assumed aqueous) is varied from 12 to 6.5 Angstroms, its measured range. Since the pore is not very much wider than the distance between water molecules, the full shielding effect of water may not be present; the extreme case of a nonaqueous pore gives a force of 3.1 pN per unit charge, which represents an upper limit. When applied to mitochondrial import experiments on the protein harness, these results imply that a force of 11 plus or minus 4 pN is sufficient to catalyze the unfolding of harness during import. Comparison of these results with unfolding forces measured using atomic force microscopy suggests that the two are not inconsistent.
Shariff, Karim↗
Protein Crystal Growth Apparatus for Microgravity
Apparatus for growing protein crystals under microgravity environment includes a plurality of protein growth assemblies stacked one above the other within a canister. Each of the protein growth assemblies includes a tray having a number of spaced apart growth chambers recessed below an upper surface. the growth chambers each having an upstanding pedestal and an annular reservoir about the pedestal for receiving a wick and precipitating agents. A well is recessed below the top of each pedestal to define a protein crystal growth receptacle. A flexible membrane is positioned on the upper surface of each tray and a sealing plate is positioned above each membrane, each sealing plate having a number of bumpers corresponding in number and alignment to the pedestals for forcing the membrane selectively against the upper end of the respective pedestal to seal the reservoir and the receptacle when the sealing plate is forced down.
Carter, Daniel C.↗
Force and deflection sensor with shell membrane and optical gratings and method of manufacture
A sensor for force is formed from an elastomeric cylinder having a region with apertures. The apertures have passageways formed between them, and an optical fiber is introduced into these passageways, where the optical fiber has a grating for measurement of tension positioned in the passageways between apertures. Optionally, a temperature measurement sensor is placed in or around the elastomer for temperature correction, and if required, a copper film may be deposited in the elastomer for reduced sensitivity to spot temperature variations in the elastomer near the sensors.
Park, Yong-Lae↗
Influence of Solutocapillary Convection on Macrovoid Defect Formation in Polymeric Membranes
The focus of this research project involved the dry-cast process for polymeric membrane formation, whereby evaporation of solvent from an initially homogeneous polymer/solvent/ nonsolvent solution results in phase separation and the formation of polymer-rich and polymer-lean phases. Under certain conditions the polymer-lean phase gives rise to very large and usually undesirable, tear-drop-shaped pores (size approx. 10 - 50 microns) termed macrovoids (MVs). Although in many cases the presence of MV pores has deleterious effects on membrane performance, there are a number of innovative applications where the presence of such pores is highly desirable. Although researchers have proposed a variety of mechanisms for MV formation over the past three decades, two main hypotheses are currently favored: one asserts that MV growth can be attributed solely to diffusion (the diffusive growth hypothesis), whereas the other states that solutocapillary convection (the SC hypothesis) at the MV interface contributes to growth. The overall goal of this research was to obtain a more comprehensive understanding of the fundamental mechanism of MV growth. This research incorporates a coupled modeling and experimental approach to test a solutocapillary convection hypothesis for the growth of macrovoid pores in polymeric membranes. Specifically, we utilized a modification of the first principles model developed by two of the PIs (ARG and WBK) for dry-cast CA membranes. For the experimental component, two separate and mutually complementary approaches were used to study MV growth. In the first, membranes cast in a zero-g environment aboard the NASA KC-135 aircraft were compared with those cast on the ground to assess the effect of the buoyancy force on membrane morphology and MV size and shape. In the second approach, videomicroscopy flow visualization (VMFV) was utilized to observe MV formation and growth in real time and to assess the effect of surface tension on the MV growth dynamics. As a result of these fundamental studies, our research group advanced a new hypothesis for MV pore development in polymeric membranes.
Greenberg, Alan R.↗
Cell surface crowding is a tunable energetic barrier to cell-cell fusion
Abstract Cell-cell fusion is fundamental to processes such as muscle formation and viral infection. An essential step in fusion is close membrane apposition, but cell membranes are crowded with proteins, glycoproteins, and glycolipids, which must be cleared before a fusion pore can be nucleated. Here, we find that cell surface crowding reduces fusogenicity independent of how fusion is driven. We estimate that crowding presents an energetic barrier to membrane apposition on the scale of ~$${100k}_{{\mbox{B}}}T$$ 100 k B T , greater than that of bare membrane fusion alone. We show that increasing cell surface crowding reduces fusion efficiency of PEG-mediated and fusogen-mediated cell-cell fusion, as well as synthetic membranes under force. Interestingly, we find that differentiating myoblasts naturally decrease their surface crowding prior to fusion. In this work, we show that cell surface crowding presents an underappreciated biophysical barrier that may be tuned developmentally and could be targeted externally to control tissue-specific cell-cell fusion.
Science & Technology - Other Topics↗
Active Contact Forces Drive Nonequilibrium Fluctuations in Membrane Vesicles
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Takatori, Sho C. (ORCID:0000000278393399)↗
Non-uniform distribution of myosin-mediated forces governs red blood cell membrane curvature through tension modulation
Not Available
Alimohamadi, Haleh↗