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At least 19 records

Genomic and morphological characterization of Knufia obscura isolated from the Mars 2020 spacecraft assembly facility

Members of the family Trichomeriaceae, belonging to the Chaetothyriales order and the Ascomycota phylum, are known for their capability to inhabit hostile environments characterized by extreme temperatures, oligotrophic conditions, drought, or presence of toxic compounds. The genus Knufia encompasses many polyextremophilic species. In this report, the genomic and morphological features of the strain FJI-L2-BK-P2 presented, which was isolated from the Mars 2020 mission spacecraft assembly facility located at the Jet Propulsion Laboratory in Pasadena, California. The identification is based on sequence alignment for marker genes, multi-locus sequence analysis, and whole genome sequence phylogeny. The morphological features were studied using a diverse range of microscopic techniques (bright field, phase contrast, differential interference contrast and scanning electron microscopy). The phylogenetic marker genes of the strain FJI-L2-BK-P2 exhibited highest similarities with type strain of Knufia obscura (CBS 148926 T ) that was isolated from the gas tank of a car in Italy. To validate the species identity, whole genomes of both strains (FJI-L2-BK-P2 and CBS 148926 T ) were sequenced, annotated, and strain FJI-L2-BK-P2 was confirmed as K. obscura. The morphological analysis and description of the genomic characteristics of K. obscura FJI-L2-BK-P2 may contribute to refining the taxonomy of Knufia species. Key morphological features are reported in this K. obscura strain, resembling microsclerotia and chlamydospore-like propagules. These features known to be characteristic features in black fungi which could potentially facilitate their adaptation to harsh environments.

59 BASIC BIOLOGICAL SCIENCES↗

Description and Genome Characterization of Three Novel Fungal Strains Isolated from Mars 2020 Mission-Associated Spacecraft Assembly Facility Surfaces—Recommendations for Two New Genera and One Species

National Aeronautics and Space Administration’s (NASA) spacecraft assembly facilities are monitored for the presence of any bacteria or fungi that might conceivably survive a transfer to an extraterrestrial environment. Fungi present a broad and diverse range of phenotypic and functional traits to adapt to extreme conditions, hence the detection of fungi and subsequent eradication of them are needed to prevent forward contamination for future NASA missions. During the construction and assembly for the Mars 2020 mission, three fungal strains with unique morphological and phylogenetic properties were isolated from spacecraft assembly facilities. The reconstruction of phylogenetic trees based on several gene loci (ITS, LSU, SSU, RPB, TUB, TEF1) using multi-locus sequence typing (MLST) and whole genome sequencing (WGS) analyses supported the hypothesis that these were novel species. Here we report the genus or species-level classification of these three novel strains via a polyphasic approach using phylogenetic analysis, colony and cell morphology, and comparative analysis of WGS. The strain FJI-L9-BK-P1 isolated from the Jet Propulsion Laboratory Spacecraft Assembly Facility (JPL-SAF) exhibited a putative phylogenetic relationship with the strain Aaosphaeria arxii CBS175.79 but showed distinct morphology and microscopic features. Another JPL-SAF strain, FJII-L3-CM-DR1, was phylogenetically distinct from members of the family Trichomeriaceae and exhibited morphologically different features from the genera Lithohypha and Strelitziana. The strain FKI-L1-BK-DR1 isolated from the Kennedy Space Center facility was identified as a member of Dothideomycetes incertae sedis and is closely related to the family Kirschsteiniotheliaceae according to a phylogenetic analysis. The polyphasic taxonomic approach supported the recommendation for establishing two novel genera and one novel species. The names Aaosphaeria pasadenensis (FJI-L9-BK-P1 = NRRL 64424 = DSM 114621), Pasadenomyces melaninifex (FJII-L3-CM-DR1 = NRRL 64433 = DSM 114623), and Floridaphiala radiotolerans (FKI-L1-BK-DR1 = NRRL 64434 = DSM 114624) are proposed as type species. Furthermore, resistance to ultraviolet-C and presence of specific biosynthetic gene cluster(s) coding for metabolically active compounds are unique to these strains.

59 BASIC BIOLOGICAL SCIENCES↗

Space Nuclear Power Autonomous Control Algorithm and Control Element Test Bed

Nuclear thermal rockets are currently NASA’s preferred option for use in a manned mission to Mars in the 2040s. The communication delay between an Earth ground station and a spacecraft heading toward Mars can be up to 20 min. Therefore, controlling the nuclear rocket engine would require either a full-time reactor operator on the mission or an autonomous control system for the reactor. The latter idea of making space nuclear reactors fully autonomous has drawn more interest from stakeholders, but such an autonomous control system must be rigorously tested and validated before it is certified for human use. The cost of a full ground test for a space nuclear reactor is tremendous, so a nonnuclear mock reactor test bed was created to test and validate control elements and control algorithms for space nuclear reactors. The test bed consists of control element hardware that inputs physical measurement data into a reactor emulator to produce the reactor’s performance under steady-state, transient, and fault conditions. The control element hardware consists of six full-sized control drums equipped with servo drives and motors and is instrumented with optical encoders, resolvers, and torque sensors for drum movement characterization. In addition to the drums, a two-phase flow loop was designed and built to mimic the valves and turbomachinery associated with the propellant flow through a nuclear thermal rocket engine; components such as pressure sensors, flow meters, thermocouples, and tachometers are instrumented throughout the loop to characterize the fluid flow, valve, and turbomachinery behavior of the system. The data from the physical hardware (e.g., drum position, propellant flow rates) are input to a nuclear reactor simulator to determine the actual nuclear reactor parameters, and the data are sent back to a control algorithm to complete the control loop. The ability to conduct numerous tests of the control systems and autonomous algorithms can help validate the instrumentation and control aspects for a space nuclear reactor for every possible fault situation.

Wilson, Brandon↗

Lyα Observations of Comet C/2013 A1 (Siding Spring) Using MAVEN IUVS Echelle

The close approach of comet C/2013 A1 (Siding Spring) to Mars in 2014 October provided a unique opportunity to observe a dynamically new Oort cloud comet with potential for interaction with a planet’s atmosphere. The water-originating hydrogen coma of the comet extended to over 20 million km from the nucleus. Determining the properties of this coma contributes to characterizing the comet’s water content and production rate. The present study analyzes a unique data set of high spectral resolution UV observations of comet C/2013 A1 Siding Spring measured by the Mars Atmosphere and Volatile Evolution spacecraft. The Siding Spring observations capture Lyα emissions from the Martian corona, the interplanetary medium, as well as the cometary H and D reservoirs. The isolated cometary spectra are analyzed to reveal a velocity distribution of H atoms that are consistent with model estimates of H{sub 2}O photodissociated H emissions and of OH photodissociated H emissions, Doppler shifted from the main comet H emission line center by 18 km s{sup −1} and 8 km s{sup −1}, respectively. The variations in comet H brightness with distance from the nucleus are used to constrain cometary water production to a rate of 0.5 × 10{sup 28} molecules s{sup −1} at a time when Siding Spring was at 1.5 au, pre-perihelion.

79 ASTRONOMY AND ASTROPHYSICS↗

Report on the Analysis of Plutonium-238 Oxide, Campaign #P5PO

Oak Ridge National Laboratory (ORNL) is currently producing Plutonium-238 oxide for NASA space programs. Through the irradiation of NpO 2 pellets in the High Flux Isotope Reactor (HFIR), the production of Pu-238 oxide will be used for electrical power and heat on NASA spacecraft such as the Curiosity Mars Rover and the Voyager 1 and 2. The Transuranium Analytical Laboratory (TAL) leads the analytical effort for this project, which includes the dissolution of plutonium product oxide (PUP) and the analysis of the dissolved product solution using both radiological and inductively coupled plasma mass spectrometry (ICP-MS) techniques that are required to meet product specifications. The Transuranic Analytical Laboratory received three samples of Pu-238 oxide for analysis in January of 2022. This report documents the analytical protocols performed by the TAL along with the results produced from said analytical protocols. This report also includes a comparison with the general-purpose heat source (GPHS) specifications.

07 ISOTOPE AND RADIATION SOURCES↗

Characterizing Mars's Magnetotail Topology With Respect to the Upstream Interplanetary Magnetic Fields

The canonical picture of the magnetotail of unmagnetized planets consists of draped interplanetary magnetic fields (IMFs) forming opposite-directed lobes, separated by the current sheet. DiBraccio et al. (2018, https://doi.org/10.1029/2018GL077251) showed that Mars's magnetotail has a twist departing from this picture. Magnetohydrodynamic (MHD) results suggest that the asymmetry in how open field lines connected to the planet populate the tail causes the apparent twist. To validate this interpretation, we compare the tail topology determined from MHD simulations to that inferred from data collected by the Mars Atmosphere and Volatile EvolutioN (MAVEN) spacecraft, in particular, how each topology responds to the upstream IMF orientation. The occurrence rates for open topology from both data and MHD vary with IMF polarities in a similar fashion as the tail twisting. This suggests that Mars's crustal fields have a global effect on the magnetosphere configuration, supporting the picture of a “hybrid” magnetotail that is partly induced/draped and partly intrinsic/planetary in origin.

79 ASTRONOMY AND ASTROPHYSICS↗

First Observation of Harmonics of Magnetosonic Waves in Martian Magnetosheath Region

Abstract The present study provides an evidence for the generation of harmonics of magnetosonic waves in the Martian magnetosheath region. The wave signatures are manifested in the magnetic field measurements recorded by the fluxgate magnetometer instrument onboard the Mars Atmosphere and Volatile Evolution missioN (MAVEN) spacecraft in the dawn sector around 5–10 LT at an altitude of 4,000–6,000 kms. The wave that is observed continuously from 19.1 to 20.7 UT below the proton cyclotron frequency (f ci ≈ 46 mHz) is identified as fundamental mode of the magnetosonic wave. Whereas harmonics of the magnetosonic wave are observed during 19.7–20.3 UT at frequencies that are multiple off ci . The ambient solar wind proton density and plasma flow velocity are found to vary with a fundamental mode frequency of 46 mHz. It is noticed that the fundamental mode is mainly associated with the left‐hand (LH), and higher frequency harmonics are associated with the right‐hand (RH) circular polarizations. A clear difference in the polarization and ellipticity is noticed during the time of occurrence of harmonics. The magnetosonic wave harmonics are found to propagate in the quasi‐perpendicular directions to the ambient magnetic field. The results of linear theory and Particle‐In‐Cell simulation performed here are in agreement with the observations. The present study provides a conclusive evidence for the occurrence of harmonics of magnetosonic wave in the close vicinity of the magnetosheath region of the unmagnetized planet Mars.

Astronomy & Astrophysics↗

Characterization of high-frequency waves in the Martian magnetosphere

Context.Various high-frequency waves in the vicinity of upper-hybrid and Langmuir frequencies are commonly observed in different space plasma environments. Such waves and fluctuations have been reported in the magnetosphere of the Earth, a planet with an intrinsic strong magnetic field. Mars has no intrinsic magnetic field and, instead, it possesses a weak induced magnetosphere, which is highly dynamic due to direct exposure to the solar wind. In the present paper, we investigate the presence of high-frequency plasma waves in the Martian plasma environment by making use of the high-resolution electric field data from the Mars Atmosphere and Volatile Evolution missioN (MAVEN) spacecraft. Aims.This study aims to provide conclusive observational evidence of the occurrence of high-frequency plasma waves around the electron plasma frequency in the Martian magnetosphere. We observe two distinct wave modes with frequency below and above the electron plasma frequency. The characteristics of these high-frequency waves are quantified and presented here. We discuss the generation of possible wave modes by taking into account the ambient plasma parameters in the region of observation. Methods.We have made use of the medium frequency (100 Hz–32 kHz) burst mode-calibrated electric field data from the Langmuir Probe and Waves instrument on board NASA’s MAVEN mission. Due to the weak magnetic field strength, the electron gyro-frequency is much lower than the electron plasma frequency, which implies that the upper-hybrid and Langmuir waves have comparable frequencies. A total of 19 wave events with wave activities around electron plasma frequency were identified by examining high-resolution spectrograms of the electric field. Results.These waves were observed around 5 LT when MAVEN crossed the magnetopause boundary and entered the magnetosheath region. These waves are either a broadband- or narrowband-type with distinguishable features in the frequency domain. The narrowband-type waves have spectral peak above the electron plasma frequency. However, in the case of broadband-type waves, the spectral peak always occurred below the electron plasma frequency. The broadband waves consistently show a periodic modulation of 8–14 ms. Conclusions.The high-frequency narrowband-type waves observed above the electron plasma frequency are believed to be associated with upper-hybrid or Langmuir waves. However, the physical mechanism responsible for the generation of broadband-type waves and the associated 8–14 ms modulation remain unexplained and further investigation is required.

Astronomy & Astrophysics↗

Seismic constraints from a Mars impact experiment using InSight and Perseverance

NASA’s InSight (Interior Exploration using Seismic Investigations, Geodesy and Heat Transport) mission has operated a sophisticated suite of seismology and geophysics instruments on the surface of Mars since its arrival in 2018. On 18 February 2021, we attempted to detect the seismic and acoustic waves produced by the entry, descent and landing of the Perseverance rover using the sensors onboard the InSight lander. Similar observations have been made on Earth using data from both crewed and uncrewed spacecraft, and on the Moon during the Apollo era, but never before on Mars or another planet. This was the only seismic event to occur on Mars since InSight began operations that had an a priori known and independently constrained timing and location. It therefore had the potential to be used as a calibration for other marsquakes recorded by InSight. Here we report that no signal from Perseverance’s entry, descent and landing is identifiable in the InSight data. Nonetheless, measurements made during the landing window enable us to place constraints on the distance–amplitude relationships used to predict the amplitude of seismic waves produced by planetary impacts and place in situ constraints on Martian impact seismic efficiency (the fraction of the impactor kinetic energy converted into seismic energy).

79 ASTRONOMY AND ASTROPHYSICS↗

Questions to Heaven

Benjamin Fernando and colleagues report on the international cooperation involved InSight's attempt to gather seismic data from the arrival at Mars of China's Zhurong rover. In one of the first collaborations of its kind, scientists working on China's Tianwen-1 mission and NASA's InSight spacecraft worked together to try and detect the seismic signatures of the Zhurong Rover's arrival at Mars. Although no signal was recorded, we present here the results of the experiment in the hope that it may act as a guide for future collaborations of this kind.

79 ASTRONOMY AND ASTROPHYSICS↗

MULTI-MISSION THERMOELECTRIC GENERATOR ASSEMBLY TESTING AND LAUNCH OPERATIONS FOR MARS 2020

The Space Nuclear Power and Isotope Technologies (SNPIT) division at Idaho National Laboratory (INL) fuels, performs acceptance testing, and provides spacecraft integration support of Radioisotope Power Systems (RPS) in support of National Aeronautics and Space Administration (NASA) missions. Recently the SNPIT team completed assembly, testing and launch support of the Multi-Mission Radioisotope Thermoelectric Generator (MMRTG) for the Mars 2020 Perseverance Rover mission.

07 ISOTOPE AND RADIATION SOURCES↗

MULTI-MISSION THERMOELECTRIC GENERATOR ASSEMBLY TESTING AND LAUNCH OPERATIONS FOR MARS 2020

The Space Nuclear Power and Isotope Technologies (SNPIT) division at Idaho National Laboratory (INL) fuels, performs acceptance testing, and provides spacecraft integration support of Radioisotope Power Systems (RPS) in support of National Aeronautics and Space Administration (NASA) missions. Recently the SNPIT team completed assembly, testing and launch support of the Multi-Mission Radioisotope Thermoelectric Generator (MMRTG) for the Mars 2020 Perseverance Rover mission.

07 ISOTOPE AND RADIATION SOURCES↗

Tarda and Tagish Lake: Samples from the same outer Solar System asteroid and implications for D- and P-type asteroids

Here, we report a comprehensive study of the ungrouped type 2 carbonaceous chondrite, Tarda, which fell in Morocco in 2020. This meteorite exhibits substantial similarities to Tagish Lake, Wisconsin Range 91600, and Meteorite Hills 00432, which are generally considered to have originated from a D-type asteroid(s). We constrain the compositions and petrologies of the materials present in a potential sample of a D-type asteroid by reporting the petrography, bulk chemical compositions, bulk H, C, N, Cr, and Ti isotopic compositions, reflectance spectra, and in situ chemical compositions of metals, sulfides, carbonates, and FeO-poor and FeO-rich chondrule silicates of Tarda. We also present new data for Tagish Lake. We then compare Tarda with the other Tagish Lake-like meteorites. Tarda and Tagish Lake appear to be from the same parent body, as demonstrated by their similar petrologies (modal abundances, chondrule sizes), mineral compositions, bulk chemical and isotopic compositions, and reflectance spectra. While the two other Tagish Lake-like meteorites, Wisconsin Range 91600 and Meteorite Hills 00432, show some affinities to Tagish Lake and Tarda, they also share similar characteristics to the Mighei-like carbonaceous (CM) chondrites, warranting further study. Similarities in reflectance spectra suggest that P-type asteroids 65 Cybele and 76 Freia are potential parent bodies of Tarda and the Tagish Lake-like meteorites, or at least have similar surface materials. Since upcoming spacecraft missions will spectrally survey D-type, P-type, and C-type Trojan asteroids (NASA's Lucy) and spectrally study and return samples from Mars' moon Phobos (JAXA's Martian Moons eXploration mission), which is spectrally similar to D-type asteroids, these meteorites are of substantial scientific interest. Furthermore, since Tarda closely spectrally matches P-type asteroids (but compositionally matches the D-type asteroid like Tagish Lake meteorite), P-type and D-type asteroids may represent fragments of the same or similar parent bodies.

58 GEOSCIENCES↗

VLBI Data Processing on Coronal Radio-sounding Experiments of Mars Express

The ESA’s Mars Express solar corona experiments were performed at two solar conjunctions in the years 2015 and 2017 by a number of radio telescopes in the European VLBI Network. This paper presents the methods to measure the frequency and phase fluctuations of the spacecraft radio signal, and the applications to study the characteristics of the plasma turbulence effects on the signal at a single station and at multiple stations via cross correlation. The power spectra of the frequency fluctuations observed between 4.9 and 76.3 R {sub s} have a power-law shape close to a Kolmogorov spectrum over the frequency interval ν {sub lo} < ν < ν {sub up}, where the nominal value of ν {sub lo} is set to 3 mHz and ν {sub up} is in the range of 0.03–0.15 Hz. The rms of the frequency fluctuations is presented as a function of the heliocentric distance. Furthermore, we analyze the variations of the electron column density fluctuations at solar offsets 4.9 R {sub s} and 9.9 R {sub s} and the cross-correlation products between the VLBI stations. The power density of the differential fluctuations between different stations decreases at ν < 0.01 Hz. Finally, the fast flow speeds of solar wind >700 km s{sup -1} are derived from the cross correlation of frequency fluctuations at ν < 0.01 Hz. The fast flow speeds of solar wind correspond to the high heliolatitude of the coronal region that the radio rays passed. The VLBI observations and analysis methods can be used to study the electron column density fluctuations and the turbulence at multiple spatial points in the inner solar wind by providing multiple lines of sight between the Earth and the spacecraft.

71 CLASSICAL AND QUANTUM MECHANICS, GENERAL PHYSIC↗

Listening for the Landing: Seismic Detections of Perseverance's Arrival at Mars With InSight

The entry, descent, and landing (EDL) sequence of NASA's Mars 2020 Perseverance Rover will act as a seismic source of known temporal and spatial localization. We evaluate whether the signals produced by this event will be detectable by the InSight lander (3,452 km away), comparing expected signal amplitudes to noise levels at the instrument. Modeling is undertaken to predict the propagation of the acoustic signal (purely in the atmosphere), the seismoacoustic signal (atmosphere-to-ground coupled), and the elastodynamic seismic signal (in the ground only). Our results suggest that the acoustic and seismoacoustic signals, produced by the atmospheric shock wave from the EDL, are unlikely to be detectable due to the pattern of winds in the martian atmosphere and the weak air-to-ground coupling, respectively. However, the elastodynamic seismic signal produced by the impact of the spacecraft's cruise balance masses on the surface may be detected by InSight. The upper and lower bounds on predicted ground velocity at InSight are 2.0 × 10 -14 and 1.3 × 10 -10 m s -1 . The upper value is above the noise floor at the time of landing 40% of the time on average. The large range of possible values reflects uncertainties in the current understanding of impact-generated seismic waves and their subsequent propagation and attenuation through Mars. Uncertainty in the detectability also stems from the indeterminate instrument noise level at the time of this future event. A positive detection would be of enormous value in constraining the seismic properties of Mars, and in improving our understanding of impact-generated seismic waves.

58 GEOSCIENCES↗

Space radiation measurements during the Artemis I lunar mission

Space radiation is a notable hazard for long-duration human spaceflight. Associated risks include cancer, cataracts, degenerative diseases and tissue reactions from large, acute exposures. Space radiation originates from diverse sources, including galactic cosmic rays, trapped-particle (Van Allen) belts5 and solar-particle events. Previous radiation data are from the International Space Station and the Space Shuttle in low-Earth orbit protected by heavy shielding and Earth’s magnetic field and lightly shielded interplanetary robotic probes such as Mars Science Laboratory and Lunar Reconnaissance Orbiter. Limited data from the Apollo missions and ground measurements with substantial caveats are also available. Here we report radiation measurements from the heavily shielded Orion spacecraft on the uncrewed Artemis I lunar mission. At differing shielding locations inside the vehicle, a fourfold difference in dose rates was observed during proton-belt passes that are similar to large, reference solar-particle events. Interplanetary cosmic-ray dose equivalent rates in Orion were as much as 60% lower than previous observations. Furthermore, a change in orientation of the spacecraft during the proton-belt transit resulted in a reduction of radiation dose rates of around 50%. These measurements validate the Orion for future crewed exploration and inform future human spaceflight mission design.

79 ASTRONOMY AND ASTROPHYSICS↗

Predicting Biological Cleanliness: An Empirical Bayes Approach for Spacecraft Bioburden Accounting

To comply with the international planetary protection policy set forth by the Committee on Space Research and NASA Agency level requirements, spacecraft destined to biologically sensitive planetary bodies have to minimize terrestrial biological contamination. Analysis, testing and inspection are the standard forward verification activities that are used to demonstrate compliance with the biological contamination requirements. For testing of spacecraft surface areas, a swab or wipe sample is collected from surfaces prior to last access and subsequently processed in the lab using NASA Approved Planetary Protection Methods for Culture Based Assays. Raw data resulting from this assay is then statistically treated employing a mathematical paradigm stemming from the 1970’s Viking Lander Project to generate the bioburden density and total microbial bioburden present. This standard approach arbitrarily accounts for error and provides an upper conservative bound as it reports the maximum number of spores estimated to be present on flight hardware surfaces. A bioburden density estimate factors in the following variables: the observed bioburden count, representative volume processed, sampling efficiencies. Notably, to account for error in the approach, a 0 observed count is arbitrarily changed to a count of 1 for each hardware grouping. The data generated by spacecraft bioburden verification campaigns in the past have resulted in <80% of wipes and <90% of swabs containing a bioburden count of 0. As such, having a robust and well documented statistical approach for dealing with the probability of low incident rates is necessary to be able to estimate spacecraft bioburden. Being able to statistically describe the bioburden distribution and associated confidence level is a gamechanger for the development of bioburden allocations during mission design and will allow for tighter management of risk throughout spacecraft build. Thus, Empirical Bayes statistical approach was evaluated to estimate the microbial bioburden on spacecraft to mitigate the aforementioned mathematical concerns and provide a probabilistic bioburden distribution of the flight hardware surface. For application of this approach to performing bioburden calculations, a range of non-informative prior assumptions on hardware surfaces are explored for Bayesian analyses while informative priors using posterior distributions from prior assays are utilized for Empirical Bayes analyses. Several non-informative priors are currently under investigation to assess fitness including use of these priors to serve as a foundation to build off of NASA specification values or a basis of risk to account for unknowns during the integration and testing process. Informative priors under consideration are generated using sampled bioburden values from hardware originating within like processing environments (e.g. vendor cleaning process or similar assembly process), temporal spacecraft status events as a prediction for hardware cleanliness of future samples, and heritage system bioburden actuals to predict allocation for subsequent missions. Informative priors and probabilistic bioburden distributions are then validated using data sets from the Mars Exploration Rover, Mars Science Laboratory, and InSight missions. Using Empirical Bayes approach to generate a probabilistic bioburden distribution as demonstrated through mission use cases provides a valid approach for use in the end-to-end requirements verification process.

97 MATHEMATICS AND COMPUTING↗