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At least 19 records

Population dynamics of transgenic strain Escherichia coli Z905/pPHL7 in freshwater and saline lake water microcosms with differing microbial community structures

Populations of Escherichia coli Z905/pPHL7, a transgenic microorganism, were heterogenic in the expression of plasmid genes when adapting to the conditions of water microcosms of various mineralization levels and structure of microbial community. This TM has formed two subpopulations (ampicillin-resistant and ampicillin-sensitive) in every microcosm. Irrespective of mineralization level of a microcosm, when E. coli Z905/pPHL7 alone was introduced, the ampicillin-resistant subpopulation prevailed, while introduction of the TM together with indigenous bacteria led to the dominance of the ampicillin-sensitive subpopulation. A high level of lux gene expression maintained longer in the freshwater microcosms than in sterile saline lake water microcosms. A horizontal gene transfer has been revealed between the jointly introduced TM and Micrococcus sp. 9/pSH1 in microcosms with the Lake Shira sterile water. c2005 COSPAR. Published by Elsevier Ltd. All rights reserved.

Ecosystem↗

Alteration of Rock Fragments from Columbia River Basalt Microcosms

During an earlier study, microorganisms were grown microcosms consisting of sterilized chips of Columbia River Basalt (CRB) and natural CRB ground water with its natural microflora; environmental conditions simulated a deep subsurface, anaerobic, dark environment. Subsequent scanning and transmission electron microscope (SEM and TEM) studies revealed the presence of several types of bacteria and biofilm, some of which were mineralized. Some of these biological features are very similar to possible biogenic features found in two meteorites from Mars, ALH84001 (found in Antarctica) and Nakhla (observed to fall in Egypt). Both ALH84001 and Nakhla contain traces of low-temperature aqueous alteration of silicates, oxides, and sulfides. The goals of this study are to use high-resolution field-emission SEM (FE-SEM) to examine the CRB samples for evidence of alteration features similar to those in the martian meteorites, to determine the extent of alteration during the CRB microcosm experiments, and to determine whether effects of biological activity can be distinguished from inorganic effects.

Wentworth, Susan J.↗

Changes in Arctic and Antarctic Sea Ice as a Microcosm of Global Climate Change

Polar sea ice is a key element of the climate system and has now been monitored through satellite observations for over three and a half decades. The satellite observations reveal considerable information about polar ice and its changes since the late 1970s, including a prominent downward trend in Arctic sea ice coverage and a much lesser upward trend in Antarctic sea ice coverage, illustrative of the important fact that climate change entails spatial contrasts. The decreasing ice coverage in the Arctic corresponds well with contemporaneous Arctic warming and exhibits particularly large decreases in the summers of 2007 and 2012, influenced by both preconditioning and atmospheric conditions. The increasing ice coverage in the Antarctic is not as readily explained, but spatial differences in the Antarctic trends suggest a possible connection with atmospheric circulation changes that have perhaps been influenced by the Antarctic ozone hole. The changes in the polar ice covers and the issues surrounding those changes have many commonalities with broader climate changes and their surrounding issues, allowing the sea ice changes to be viewed in some important ways as a microcosm of global climate change.

Arctic↗

Effects of Potassium Permanganate Oxidation on Subsurface Microbial Activity

In situ chemical oxidation has the potential for degrading large quantities of organic contaminants and can be more effective and timely than traditional ex situ treatment methods. However, there is a need to better characterize the potential effects of this treatment on natural processes. This study focuses on potential inhibition to anaerobic dechlorination of trichloroethene (TCE) in soils from a large manufacturing facility as a result of in situ oxidation using potassium permanganate (KMn04)Previous microcosm studies established that natural attenuation occurs on-site and that it is enhanced by the addition of ethanol to the system. A potential remediation scheme for the site involves the use of potassium permanganate to reduce levels of TCE in heavily contaminated areas, then to inject ethanol into the system to "neutralize" excess oxidant and enhance microbial degradation. However, it is currently unknown whether the exposure of indigenous microbial populations to potassium permanganate may adversely affect biological reductive dechlorination by these microorganisms. Consequently, additional microcosm studies were conducted to evaluate this remediation scheme and assess the effect of potassium permanganate addition on biological reductive dechlorination of TCE. Samples of subsurface soil and groundwater were collected from a TCE-impacted area of the site. A portion of the soil was pretreated with nutrients and ethanol to stimulate microbial activity, while the remainder of the soil was left unamended. Soil/groundwater microcosms were prepared in sealed vials using the nutrient-amended and unamended soils, and the effects of potassium permanganate addition were evaluated using two permanganate concentrations (0.8 and 2.4 percent) and two contact times (1 and 3 weeks). TCE was then re-added to each microcosm and TCE and dichloroethene (DCE) concentrations were monitored to determine the degree to which microbial dechlorination occurred following chemical oxidation. Evidence of microbial degradation was generally detected within four weeks after TCE addition. Increases in DCE concentrations were consistent with decreases in TCE. The concentration of TCE in the nutrient-amended samples exposed to 2.4% KMnO4 for one week degraded somewhat more slowly than the samples exposed to the 0.8% KMnO4. The rates of degradation did not correlate with the length of KMn04 exposure for the nutrient-amended microcosms. Microbial degradation of TCE in the unamended microcosms was generally similar to that observed in the nutrient-amended microcosms. One treatment condition (unamended, one week exposure, 2.4% KMnO4) was exposed to elevated levels of ethanol and showed little evidence of degradation. It is suspected that the high levels of ethanol were toxic to the microorganisms. The results of the study indicate that exposure of indigenous soil and groundwater microbial populations to KMnO4 at concentrations of 0.8 to 2.4% do not impair the ability of the microbial populations to dechlorinate TCE. Consequently, the combination of chemical oxidation followed by enhanced biological reductive dechlorination appears to be a viable remedial strategy for highly-impacted subsurface areas of the site.

Rowland, Martin A.↗

Mineralization of Bacteria in Terrestrial Basaltic Rocks: Comparison With Possible Biogenic Features in Martian Meteorite Allan Hills 84001

The identification of biogenic features altered by diagenesis or mineralization is important in determining whether specific features in terrestrial rocks and in meteorites may have a biogenic origin. Unfortunately, few studies have addressed the formation of biogenic features in igneous rocks, which may be important to these phenomena, including the controversy over possible biogenic features in basaltic martian meteorite ALH84001. To explore the presence of biogenic features in igneous rocks, we examined microcosms growing in basaltic small-scale experimental growth chambers or microcosms. Microbial communities were harvested from aquifers of the Columbia River Basalt (CRB) group and grown in a microcosm containing unweathered basalt chips and groundwater (technique described in. These microcosms simulated natural growth conditions in the deep subsurface of the CRB, which should be a good terrestrial analog for any putative martian subsurface ecosystem that may have once included ALH84001. Here we present new size measurements and photomicrographs comparing the putative martian fossils to biogenic material in the CRB microcosms. The range of size and shapes of the biogenic features on the CRB microcosm chips overlaps with and is similar to those on ALH84001 chips. Although this present work does not provide evidence for the biogenicity of ALH84001 features, we believe that, based on criteria of size, shape, and general morphology, a biogenic interpretation for the ALH84001 features remains plausible.

Thomas-Keprta, K. L.↗

Effect of shadowing on survival of bacteria under conditions simulating the Martian atmosphere and UV radiation

Spacecraft-associated spores and four non-spore-forming bacterial isolates were prepared in Atacama Desert soil suspensions and tested both in solution and in a desiccated state to elucidate the shadowing effect of soil particulates on bacterial survival under simulated Martian atmospheric and UV irradiation conditions. All non-spore-forming cells that were prepared in nutrient-depleted, 0.2-μm-filtered desert soil (DSE) microcosms and desiccated for 75 days on aluminum died, whereas cells prepared similarly in 60-μm-filtered desert soil (DS) microcosms survived such conditions. Among the bacterial cells tested, Microbacterium schleiferi and Arthrobacter sp. exhibited elevated resistance to 254-nm UV irradiation (low-pressure Hg lamp), and their survival indices were comparable to those of DS- and DSE-associated Bacillus pumilus spores. Desiccated DSE-associated spores survived exposure to full Martian UV irradiation (200 to 400 nm) for 5 min and were only slightly affected by Martian atmospheric conditions in the absence of UV irradiation. Although prolonged UV irradiation (5 min to 12 h) killed substantial portions of the spores in DSE microcosms (~5- to 6-log reduction with Martian UV irradiation), dramatic survival of spores was apparent in DS-spore microcosms. The survival of soil-associated wild-type spores under Martian conditions could have repercussions for forward contamination of extraterrestrial environments, especially Mars.

Venkateswaran, Kasthuri↗

Factors that control the stable carbon isotopic composition of methane produced in an anoxic marine sediment

The carbon isotopic composition of methane produced in anoxic marine sediment is controlled by four factors: (1) the pathway of methane formation, (2) the isotopic composition of the methanogenic precursors, (3) the isotope fractionation factors for methane production, and (4) the isotope fractionation associated with methane oxidation. The importance of each factor was evaluated by monitoring stable carbon isotope ratios in methane produced by a sediment microcosm. Methane did not accumulate during the initial 42-day period when sediment contained sulfate, indicating little methane production from 'noncompetitive' substrates. Following sulfate depletion, methane accumulation proceeded in three distinct phases. First, CO2 reduction was the dominant methanogenic pathway and the isotopic composition of the methane produced ranged from -80 to -94 per thousand. The acetate concentration increased during this phase, suggesting that acetoclastic methanogenic bacteria were unable to keep pace with acetate production. Second, acetate fermentation became the dominant methanogenic pathway as bacteria responded to elevated acetate concentrations. The methane produced during this phase was progressively enriched in C-13, reaching a maximum delta(C-13) value of -42 per thousand. Third, the acetate pool experienced a precipitous decline from greater than 5 mM to less than 20 micro-M and methane production was again dominated by CO2 reduction. The delta(C-13) of methane produced during this final phase ranged from -46 to -58 per thousand. Methane oxidation concurrent with methane production was detected throughout the period of methane accumulation, at rates equivalent to 1 to 8 percent of the gross methane production rate. Thus methane oxidation was too slow to have significantly modified the isotopic signature of methane. A comparison of microcosm and field data suggests that similar microbial interactions may control seasonal variability in the isotopic composition of methane emitted from undisturbed Cape Lookout Bight sediment.

Alperin, M. J.↗

Super-Compact Laser

Microcosm, Inc. produced the portable Farfield-2 laser for field applications that require high power pulsed illumination. The compact design was conceived through research at Goddard Space Flight Center on laser instruments for space missions to carry out geoscience studies of Earth. An exclusive license to the key NASA patent for the compact laser design was assigned to Microcosm. The FarField-2 is ideal for field applications, has low power consumption, does not need water cooling or gas supplies, and produces nearly ideal beam quality. The properties of the laser also make it effective over long distances, which is one reason why NASA developed the technology for laser altimeters that can be toted aboard spacecraft. Applications for the FarField-2 include medicine, biology, and materials science and processing, as well as diamond marking, semiconductor line-cutting, chromosome surgery, and fluorescence microscopy.

Source record↗

Cosmology without general relativity

Idealizations in cosmology with special theory of relativity - partitioned universe, microcosmic model, and similarity to Newtonian cosmology

RELATIVITY THEORY↗

Thermal control surfaces experiment (SOO69) flight systems performance

The thermal control surfaces experiment (TCSE) was the most complex hardware system aboard the Long Duration Exposure Facility (LDEF). The TCSE system consists of a scanning spectroreflectometer that measured test samples mounted on a rotatable carousel assembly. A microprocessor based data system controlled all aspects of TCSE system operation. Power was provided by four primary batteries. Flight measurement and housekeeping data were stored on a tape recorder for postflight analysis. The TCSE is a microcosm of complex electro-optical payloads being developed by NASA, DoD, and the aerospace community. The TCSE provides valuable data on the performance of these systems in space. The TCSE flight system and its excellent performance on the LDEF mission are described. A few operational anomalies were encountered and are discussed. Initial post-flight tests show that the TCSE system remains functional although some degradation in the optical measurements were observed. The results of these tests are also presented.

Wilkes, Donald R.↗

Thermal control surfaces experiment flight system performance

The Thermal Control Surfaces Experiment (TCSE) is the most complex system, other than the LDEF, retrieved after long term space exposure. The TCSE is a microcosm of complex electro-optical payloads being developed and flow by NASA and the DoD including SDI. The objective of TCSE was to determine the effects of the near-Earth orbital environment and the LDEF induced environment on spacecraft thermal control surfaces. The TCSE was a comprehensive experiment that combined in-space measurements with extensive post flight analyses of thermal control surfaces to determine the effects of exposure to the low earth orbit space environment. The TCSE was the first space experiment to measure the optical properties of thermal control surfaces the way they are routinely measured in a lab. The performance of the TCSE confirms that low cost, complex experiment packages can be developed that perform well in space.

Wilkes, Donald R.↗

Thermal control surfaces experiment flight system performance

The Thermal Control Surfaces Experiment (TCSE) is the most complex system retrieved after long term space exposure. The TCSE is a microcosm of complex electro-optical payloads being developed and flown. The objective of the TCSE on the LDEF was to determine the effects of the near-Earth orbital environment and the LDEF induced environment on spacecraft thermal control surfaces. The TCSE was a comprehensive experiment that combined in-space measurements with extensive post-flight analyses of thermal control surfaces to determine the effects of exposure to the low Earth orbit space environment. The TCSE was the first space experiment to measure the optical properties of thermal control surfaces the way they are routinely measured in the lab. The performance of the TCSE flight system on the LDEF was excellent.

Wilkes, Donald R.↗

Fully autonomous navigation for the NASA cargo transfer vehicle

A great deal of attention has been paid to navigation during the close approach (less than or equal to 1 km) phase of spacecraft rendezvous. However, most spacecraft also require a navigation system which provides the necessary accuracy for placing both satellites within the range of the docking sensors. The Microcosm Autonomous Navigation System (MANS) is an on-board system which uses Earth-referenced attitude sensing hardware to provide precision orbit and attitude determination. The system is capable of functioning from LEO to GEO and beyond. Performance depends on the number of available sensors as well as mission geometry; however, extensive simulations have shown that MANS will provide 100 m to 400 m (3(sigma)) position accuracy and 0.03 to 0.07 deg (3(sigma)) attitude accuracy in low Earth orbit. The system is independent of any external source, including GPS. MANS is expected to have a significant impact on ground operations costs, mission definition and design, survivability, and the potential development of very low-cost, fully autonomous spacecraft.

Wertz, James R.↗

Environmental monitoring of Space Shuttle launches at Kennedy Space Center - The first ten years

Space Shuttle launches produce local environmental effects through the generation of a launcher exhaust plume that in turn produces acidic depositions and acute vegetation damage in the near-field environment; fish kills have also been noted in the lagoon or impoundment near each of the launch pads. Repeated launches lead to cumulative changes in plant community composition and structure, and temporary decreases in pH due to acidification increases metal availability in soil microcosms and surface waters. Direct effects on terrestrial fauna include the mortality of birds, mammals, amphibians, and reptiles in the near-field area.

Schmalzer, Paul A.↗

Support of Helicopter 'Free Flight' Operations in the 1996 Olympics

The microcosm of activity surrounding the 1996 Olympic Games provided researchers an opportunity for demonstrating state-of-the art technology in the first large-scale deployment of a prototype digital communication/navigation/surveillance system in a confined environment. At the same time it provided an ideal opportunity for transportation officials to showcase the merits of an integrated transportation system in meeting the operational needs to transport time sensitive goods and provide public safety services under real-world conditions. Five aeronautical CNS functions using a digital datalink system were chosen for operational flight testing onboard 91 aircraft, most of them helicopters, participating in the Atlanta Short-Haul Transportation System. These included: GPS-based Automatic Dependent Surveillance, Cockpit Display of Traffic Information, Controller-Pilot Communications, Graphical Weather Information (uplink), and Automated Electronic Pilot Reporting (downlink). Atlanta provided the first opportunity to demonstrate, in an actual operating environment, key datalink functions which would enhance flight safety and situational awareness for the pilot and supplement conventional air traffic control. The knowledge gained from such a large-scale deployment will help system designers in development of a national infrastructure where aircraft would have the ability to navigate autonomously.

Branstetter, James R.↗

This New Ocean: A History of Project Mercury

When Congress created the National Aeronautics and Space Administration (NASA) in 1958, it charged NASA with the responsibility "to contribute materially to . . . the expansion of human knowledge of phenomena in the atmosphere and space" and "provide for the widest practicable and appropriate dissemination of information concerning its activities and the results thereof." NASA wisely interpreted this mandate to include responsibility for documenting the epochal progress of which it is the focus. The result has been the development of a historical program by NASA as unprecedented as the task of extending man's mobility beyond his planet. This volume is not only NASA's accounting of its obligation to disseminate information to our current generation of Americans. It also fulfills, as do all of NASA's future-oriented scientific-technological activities, the further obligation to document the present as the heritage of the future. The wide-ranging NASA history program includes chronicles of day-to-day space activities; specialized studies of particular fields within space science and technology; accounts of NASA's efforts in organization and management, where its innovations, while less known to the public than its more spectacular space shots, have also been of great significance; narratives of the growth and expansion of the space centers throughout the country, which represent in microcosm many aspects of NASA's total effort; program histories, tracing the successes- and failures- of the various projects that mark man's progress into the Space Age; and a history of NASA itself, incorporating in general terms the major problems and challenges, and the responses thereto, of our entire civilian space effort. The volume presented here is a program history, the first in a series telling of NASA's pioneering steps into the Space Age. It deals with the first American manned-spaceflight program: Project Mercury. Although some academicians might protest that this is "official" history, it is official only in the fact that it has been prepared and published with the support and cooperation of NASA. It is not "official" history in the sense of presenting a point of view supposedly that of NASA officialdom-if anyone could determine what the "point of view" of such a complex organism might be. Certainly, the authors were allowed to pursue their task with the fullest freedom and in accordance with the highest scholarly standards of the history profession.

Swenson, Loyd S., Jr.↗

Cellular Oxygen and Nutrient Sensing in Microgravity Using Time-Resolved Fluorescence Microscopy

Oxygen and nutrient sensing is fundamental to the understanding of cell growth and metabolism. This requires identification of optical probes and suitable detection technology without complex calibration procedures. Under this project Microcosm developed an experimental technique that allows for simultaneous imaging of intra- and inter-cellular events. The technique consists of frequency-domain Fluorescence Lifetime Imaging Microscopy (FLIM), a set of identified oxygen and pH probes, and methods for fabrication of microsensors. Specifications for electronic and optical components of FLIM instrumentation are provided. Hardware and software were developed for data acquisition and analysis. Principles, procedures, and representative images are demonstrated. Suitable lifetime sensitive oxygen, pH, and glucose probes for intra- and extra-cellular measurements of analyte concentrations have been identified and tested. Lifetime sensing and imaging have been performed using PBS buffer, culture media, and yeast cells as a model systems. Spectral specifications, calibration curves, and probes availability are also provided in the report.

Szmacinski, Henryk↗