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At least 19 records

Linking of the mini-computer Electronik-100I and NR-9821A

The means of transmitting digital information from the computer E-100I to the desk top calculator NR-9821A with the help of an intermediate carrier of information (perforated tape) is described. The means of removal of information from the computer E-100I in a form which is understandable for the NR-9821A are given. Instructions for the use and programming of the transcription of information onto magnetic tape from the perforated tape and from the keyboard of the calculator are included.

Zubkov, B. V.

A correlational analysis of the effects of changing environmental conditions on the NR atomic hydrogen maser

An extensive statistical analysis has been undertaken to determine if a correlation exists between changes in an NR atomic hydrogen muser's frequency offset and changes in environmental conditions. Data have been acquired over the past 20 months by recording the frequency offset of three NR atomic hydrogen masers along with the relative and absolute humidity, barometric pressure, and ambient temperature of the laboratory in which the masers are maintained. Correlational analyses have been performed comparing barometric pressure, humidity, and temperature with maser frequency offset as functions of time for periods ranging from 5.5 to 17 days. Semi partial correlation coefficients as large as -0.9 have been fond between barometric pressure and maser frequency offset for data covering periods as long as a week. Maser frequency offset and barometric pressure were consistently found to change simultaneously. The correlation between humidity and frequency offset is less predictable, and the resulting semi partial correlation coefficients were usually small when compared with those derived from the relationship between pressure and frequency offset. The time delay between changes in humidity and correlated changes in maser frequency offset was found to vary extensively with no predictable pattern. Analysis of temperature data indicates that, in the most current design, temperature does not significant(y affect maser frequency offset in the laboratory environment. Thus, the results of the analyses disclose a significant statistical correlation between changes in maser frequency offset and changes in barometric pressure. The statistics also reveal some correlation between humidity and frequency offset, bat for reasons to be discussed, the effects of humidity should be considered secondary to the effects of changing barometric pressure.

R. A. Dragonette

Space shuttle: Static stability and control investigation of the NR-GD/C delta wing booster (B-15B-1) and a Reusable Nuclear Stage (RNS) M = 0.6 - 4.96

Experimental aerodynamic investigations were made in the NASA/MSFC 14 x 14 inch trisonic wind tunnel on a NR-GD/C 0.0031 scale model delta wing booster (B-15B-1) and reusable nuclear stage (RNS). Three basic configurations were tested: (1) the B-15B-1 booster alone, (2) the RNS alone, and (3) the booster with the RNS mounted piggyback. Six component force and moment data were recorded for each of these configurations over an angle of attack range from -16 deg to 4 deg at zero degrees sideslip, and over an angle of sideslip range from -10 deg to 10 deg at zero and -6 degrees angle of attack. The configurations were tested over a Mach number range of 0.6 to 5.0 with a nominal Reynolds number of 6.5 million per foot except for Mach 1.2 and 3.0 where the Reynolds number were 12.4 million and 4.4 million per foot respectively.

Allen, E. C.

NR-150 adhesive development

A polyimide precursor adhesive solution, code-named NR-056X, was developed. It reproducibly gives low void, high strength bond lines at both room temperature and 589K (600 F) using either titanium or graphite/polyimide composite adherends having 12.7 mm (1/2 in.) overlaps. Lap shear samples prepared using this adhesive and composite adherends based on graphite/NR-150B2 were shown to have a high degree of resistance to such adverse environments as air at 589K (600 F), high humidity, methyl ethyl ketone and jet fuel. Bond line toughness was illustrated by the complete resistance to cracking in the wedge-crack propagation test. The available evidence indicates that wide area composite to composite or composite to metal bonding should be feasible.

Blatz, P. S.

NASA NR hydrogen maser

The Johns Hopkins University, Applied Physics Laboratory, under contract to NASA/Goddard Space Flight Center, is engineering a new generation of field operable hydrogen masers (NR) based on prior NASA NP and NX designs. These units incorporate improvements in magnetic shielding, lower noise electronics, better thermal control and have a microprocessor for operation, monitoring and diagnostic functions. They are ruggedly built for transportability and ease of service anywhere in the world.

Rueger, L. J.

Results of investigations on a 0.0405 scale model PRR version of the NR-SSV orbiter in the North American Aeronautical Laboratory low speed wind tunnel

Experimental aerodynamic investigations were conducted in a low speed wind tunnel on a scale model space shuttle vehicle (SSV) orbiter. The purpose of the test was to investigate the longitudinal and lateral-directional aerodynamic characteristics of the space shuttle orbiter. Emphasis was placed on model component, wing-glove, and wing-body fairing effects, as well as elevon, aileron, and rudder control effectiveness. Angles of attack from - 5 deg to + 30 deg and angles of sideslip of - 5 deg, 0 deg, and + 5 deg were tested. Static pressures were recorded on base, fuselage, and wing surfaces. Tufts and talc-kerosene flow visualization techniques were also utilized. The aerodynamic force balance results are presented in plotted and tabular form.

Kingsland, R. B.

Results of investigations on a 0.0405 scale model ATP version of the NR-SSV orbiter in the North American Aeronautical Laboratory low speed wind tunnel

Experimental aerodynamic investigations were conducted on a scale model space shuttle vehicle (SSV) orbiter. The purpose of the test was to investigate the longitudinal and lateral-directional aerodynamic characteristics. Emphasis was placed on model component, wing-glove, and wing-body fairing effects, as well as elevon, aileron, and rudder control effectiveness. Angles of attack from - 5 deg to + 30 deg and angles of sideslip from - 5 deg to + 10 deg were tested. Static pressures were recorded on base, fuselage, and wing surfaces. Tufts and talc-kerosene flow visualization techniques were also utilized. The aerodynamic force balance results are presented in plotted and tabular form.

Mennell, R.

Hypersonic aerodynamic characteristics of NR-ATP orbiter, orbiter with external tank, and ascent configuration

A scale model of the North American Rockwell ATP Orbiter with and without the external tank has been tested in a 22-inch helium tunnel at Mach 20 and a Reynolds number based on model length, of 2.14 times one million. Longitudinal and lateral-directional data were determined for the orbiter alone while only longitudinal characteristics and elevon roll effectiveness were investigated for the orbiter/tank combination. Oil flow and electron beam flow visualization studies were conducted for the orbiter alone, orbiter with external tank and the ascent configuration.

Ashby, G. C., Jr.

NR-150B2 adhesive development

Adhesive based polyimide solutions which are more easily processed than conventional aromatic polyimide systems and show potential for use for extended times at 589K are discussed. The adhesive system is based on a solution containing diglyme as the solvent and 2,2 bis(3',4'-dicarboxyphenyl)hexafluoropropane, paraphenylenediamine, and oxydianiline. The replacement of N-methylpyrrolidone with diglyme as the solvent was found to improve the adhesive strengths of lap shear samples and simplify the processing conditions for bonding both titanium and graphite fiber/polyimide matrix resin composites. Information was obtained on the effects of various environments including high humidity, immersion in jet fuel and methylethylketone on aluminum filled adhesive bonds. The adhesive was also evaluated in wide area bonds and flatwise tensile specimens using titanium honeycomb and composite face sheets. It was indicated that the developed adhesive system has the potential for use in applications requiring long term exposure to at least 589K (600 F).

Blatz, P. S.

Inhibition of nitrate transport by anti-nitrate reductase IgG fragments and the identification of plasma membrane associated nitrate reductase in roots of barley seedlings

Membrane associated nitrate reductase (NR) was detected in plasma membrane (PM) fractions isolated by aqueous two-phase partitioning from barley (Hordeum vulgare L. var CM 72) roots. The PM associated NR was not removed by washing vesicles with 500 millimolar NaCl and 1 millimolar EDTA and represented up to 4% of the total root NR activity. PM associated NR was stimulated up to 20-fold by Triton X-100 whereas soluble NR was only increased 1.7-fold. The latency was a function of the solubilization of NR from the membrane. NR, solubilized from the PM fraction by Triton X-100 was inactivated by antiserum to Chlorella sorokiniana NR. Anti-NR immunoglobulin G fragments purified from the anti-NR serum inhibited NO3- uptake by more than 90% but had no effect on NO2- uptake. The inhibitory effect was only partially reversible; uptake recovered to 50% of the control after thorough rinsing of roots. Preimmune serum immunoglobulin G fragments inhibited NO3- uptake 36% but the effect was completely reversible by rinsing. Intact NR antiserum had no effect on NO3- uptake. The results present the possibility that NO3- uptake and NO3- reduction in the PM of barley roots may be related.

NASA Discipline Number 61-10

Comparative induction of nitrate reductase by nitrate and nitrite in barley leaves

The comparative induction of nitrate reductase (NR) by ambient NO3- and NO2- as a function of influx, reduction (as NR was induced) and accumulation in detached leaves of 8-day-old barley (Hordeum vulgare L.) seedlings was determined. The dynamic interaction of NO3- influx, reduction and accumulation on NR induction was shown. The activity of NR, as it was induced, influenced its further induction by affecting the internal concentration of NO3-. As the ambient concentration of NO3- increased, the relative influences imposed by influx and reduction on NO3- accumulation changed with influx becoming a more predominant regulant. Significant levels of NO3- accumulated in NO2(-)-fed leaves. When the leaves were supplied cycloheximide or tungstate along with NO2-, about 60% more NO3- accumulated in the leaves than in the absence of the inhibitors. In NO3(-)-supplied leaves NR induction was observed at an ambient concentration of as low as 0.02 mM. No NR induction occurred in leaves supplied with NO2- until the ambient NO2- concentration was 0.5 mM. In fact, NR induction from NO2- solutions was not seen until NO3- was detected in the leaves. The amount of NO3- accumulating in NO2(-)-fed leaves induced similar levels of NR as did equivalent amounts of NO3- accumulating from NO3(-)-fed leaves. In all cases the internal concentration of NO3-, but not NO2-, was highly correlated with the amount of NR induced. The evidence indicated that NO3- was a more likely inducer of NR than was NO2-.

NASA Program CELSS

Evidence for a plasma-membrane-bound nitrate reductase involved in nitrate uptake of Chlorella sorokiniana

Anti-nitrate-reductase (NR) immunoglobulin-G (IgG) fragments inhibited nitrate uptake into Chlorella cells but had no affect on nitrate uptake. Intact anti-NR serum and preimmune IgG fragments had no affect on nitrate uptake. Membrane-associated NR was detected in plasma-membrane (PM) fractions isolated by aqueous two-phase partitioning. The PM-associated NR was not removed by sonicating PM vesicles in 500 mM NaCl and 1 mM ethylenediaminetetraacetic acid and represented up to 0.8% of the total Chlorella NR activity. The PM NR was solubilized by Triton X-100 and inactivated by Chlorella NR antiserum. Plasma-membrane NR was present in ammonium-grown Chlorella cells that completely lacked soluble NR activity. The subunit sizes of the PM and soluble NRs were 60 and 95 kDa, respectively, as determined by sodium-dodecyl-sulfate electrophoresis and western blotting.

Non-NASA Center

Tropical Waves and the Quasi-Biennial Oscillation in a 7-km Global Climate Simulation

This study investigates tropical waves and their role in driving a quasi-biennial oscillation (QBO)-like signal in stratospheric winds in a global 7-km-horizontal-resolution atmospheric general circulation model. The Nature Run (NR) is a 2-year global mesoscale simulation of the Goddard Earth Observing System Model, version 5 (GEOS-5). In the tropics, there is evidence that the NR supports a broad range of convectively generated waves. The NR precipitation spectrum resembles the observed spectrum in many aspects, including the preference for westward-propagating waves. However, even with very high horizontal resolution and a healthy population of resolved waves, the zonal force provided by the resolved waves is still too low in the QBO region and parameterized gravity wave drag is the main driver of the NR QBO-like oscillation (NRQBO). The authors suggest that causes include coarse vertical resolution and excessive dissipation. Nevertheless, the very-high-resolution NR provides an opportunity to analyze the resolved wave forcing of the NR-QBO. In agreement with previous studies, large-scale Kelvin and small-scale waves contribute to the NRQBO driving in eastward shear zones and small-scale waves dominate the NR-QBO driving in westward shear zones. Waves with zonal wavelength,1000 km account for up to half of the small-scale (,3300 km) resolved wave forcing in eastward shear zones and up to 70% of the small-scale resolved wave forcing in westward shear zones of the NR-QBO.

NR-QBO

Identification of Ser-543 as the major regulatory phosphorylation site in spinach leaf nitrate reductase

Spinach leaf NADH:nitrate reductase (NR) responds to light/dark signals and photosynthetic activity in part as a result of rapid regulation by reversible protein phosphorylation. We have identified the major regulatory phosphorylation site as Ser-543, which is located in the hinge 1 region connecting the cytochrome b domain with the molybdenum-pterin cofactor binding domain of NR, using recombinant NR fragments containing or lacking the phosphorylation site sequence. Studies with NR partial reactions indicated that the block in electron flow caused by phosphorylation also could be localized to the hinge 1 region. A synthetic peptide (NR6) based on the phosphorylation site sequence was phosphorylated readily by NR kinase (NRk) in vitro. NR6 kinase activity tracked the ATP-dependent inactivation of NR during several chromatographic steps and completely inhibited inactivation/phosphorylation of native NR in vitro. Two forms of NRk were resolved by using anion exchange chromatography. Studies with synthetic peptide analogs indicated that both forms of NRk had similar specificity determinants, requiring a basic residue at P-3 (i.e., three amino acids N-terminal to the phosphorylated serine) and a hydrophobic residue at P-5. Both forms are strictly calcium dependent but belong to distinct families of protein kinases because they are distinct immunochemically.

NASA Discipline Plant Biology