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Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

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At least 19 records

microRNA-29a inhibition induces Gab1 upregulation to protect OB-6 human osteoblasts from hydrogen peroxide

Highlights: • Gab1 is required for H{sub 2}O{sub 2}-induced Akt activation in OB-6 osteoblasts. • miR-29a inhibition upregulates Gab1 to protect OB-6 cells from H{sub 2}O{sub 2}. • Gab1 over-expression promotes Akt activation to inhibit H{sub 2}O{sub 2}-induced OB-6 cell death. • Gab1 is the direct and primary target of antagomiR-29a in mediating its anti-H{sub 2}O{sub 2} actions. The present study determines the role of the Gab1 in hydrogen peroxide (H{sub 2}O{sub 2})-induced death of human osteoblasts. We show that Gab1 is required for H{sub 2}O{sub 2}-induced Akt activation to promote osteoblast survival. In OB-6 human osteoblasts, Gab1 silencing (by targeted-shRNA) or complete knockout (by CRISPR-Cas9 KO plasmid) largely attenuated Akt activation by H{sub 2}O{sub 2}. Gab1-depleted OB-6 cells were more vulnerable to H{sub 2}O{sub 2}. Conversely, forced over-expression of Gab1 by an adenovirus vector increased Akt activation to protect OB-6 cells from H{sub 2}O{sub 2}. Significantly, the anti-sense of microRNA-29a (“antagomiR-29a”) induced Gab1 expression to facilitate H{sub 2}O{sub 2}-induced Akt activation, which protected OB-6 cells from apoptosis. AntagomiR-29a was however ineffective in Gab1-deficient and Akt-inhibited OB-6 cells. Forced over-expression of miR-29a induced Gab1 downregulation to inhibit H{sub 2}O{sub 2}-induced Akt activation, causing enhanced OB-6 cell death. miR-29a-induced actions were abolished by an adenovirus constitutively-active Akt1 (Ad-caAkt1) in OB-6 cells. Together, microRNA-29a inhibition induces Gab1 upregulation and Akt activation to protect OB-6 osteoblasts from H{sub 2}O{sub 2}.

60 APPLIED LIFE SCIENCES↗

Structural basis of tRNA recognition by the widespread OB fold

The widespread oligonucleotide/oligosaccharide-binding (OB)-fold recognizes diverse substrates from sugars to nucleic acids and proteins, and plays key roles in genome maintenance, transcription, translation, and tRNA metabolism. OB-containing bacterial Trbp and yeast Arc1p proteins are thought to recognize the tRNA elbow or anticodon regions. Here we report a 2.6 Å co-crystal structure of Aquifex aeolicus Trbp111 bound to tRNA Ile , which reveals that Trbp recognizes tRNAs solely by capturing their 3' ends. Structural, mutational, and biophysical analyses show that the Trbp/EMAPII-like OB fold precisely recognizes the single-stranded structure, 3' terminal location, and specific sequence of the 3' CA dinucleotide — a universal feature of mature tRNAs. Arc1p supplements its OB – tRNA 3' end interaction with additional contacts that involve an adjacent basic region and the tRNA body. This study uncovers a previously unrecognized mode of tRNA recognition by an ancient protein fold, and provides insights into protein-mediated tRNA aminoacylation, folding, localization, trafficking, and piracy.

59 BASIC BIOLOGICAL SCIENCES↗

AmeriFlux FLUXNET-1F CA-Obs Saskatchewan - Western Boreal, Mature Black Spruce

This is the AmeriFlux Management Project (AMP) created FLUXNET-1F version of the carbon flux data for the site CA-Obs Saskatchewan - Western Boreal, Mature Black Spruce. This is the FLUXNET version of the carbon flux data for the site CA-Obs Saskatchewan - Western Boreal, Mature Black Spruce produced by applying the standard ONEFlux (1F) software. Site Description - 53.98717° N, 105.11779° W, elavation of 628.94 m, BOREAS 1994, 1996, BERMS climate measurements began Dec. 1996 and flux measurements in Apr. 1999

Black, T. Andrew↗

AmeriFlux CA-Obs Saskatchewan - Western Boreal, Mature Black Spruce

This is the AmeriFlux version of the carbon flux data for the site CA-Obs Saskatchewan - Western Boreal, Mature Black Spruce. Site Description - 53.98717° N, 105.11779° W, elavation of 628.94 m, BOREAS 1994, 1996, BERMS climate measurements began Dec. 1996 and flux measurements in Apr. 1999

Black, T. Andrew↗

Angular Momentum Evolution of Young Stars in the nearby Scorpius–Centaurus OB Association

We report the results of a study of archival SuperWASP light curves for stars in Scorpius–Centaurus (Sco–Cen), the nearest OB association. We use SuperWASP time-series photometry to extract rotation periods for 189 candidate members of the Sco–Cen complex and verify that 162 of those are members of the classic Sco–Cen subgroups of Upper Scorpius (US), Upper Centaurus-Lupus (UCL), and Lower Centaurus-Crux (LCC). This study provides the first measurements of rotation periods explicitly for large samples of pre-main-sequence (pre-MS) stars spanning the UCL and LCC subgroups. Our final sample of 157 well-characterized pre-MS stars spans ages of ∼10–20 Myr, spectral types of ∼F3–M0, and masses of M ≃ 0.3–1.5 M{sub ⊙}{sup N}. For this sample, we find a distribution of stellar rotation periods with a median of P {sub rot} ≃ 2.4 days, an overall range of 0.2 < P {sub rot} < 8 days, and a fairly well-defined mass-dependent upper envelope of rotation periods. This distribution of periods is consistent with recently developed stellar angular momentum evolution models. These data are significant because they represent an undersampled age range and the number of measurable rotation periods is large compared to recent studies of other regions. We also search for new examples of eclipsing disk or ring systems analogous to 1SWASP J140747.93-394542.6 (J1407), but find none. Our survey yielded five eclipsing binaries, but only one appears to be physically associated with the Sco–Cen complex. V2394 Oph is a heavily reddened (A {sub V} ≃ 5 mag) massive contact binary in the LDN 1689 cloud whose Gaia astrometry is clearly consistent with kinematic membership with the Ophiuchus star-forming region.

79 ASTRONOMY AND ASTROPHYSICS↗

Occupant behavior models: A critical review of implementation and representation approaches in building performance simulation programs

Occupant behavior (OB) in buildings is a leading factor influencing energy use in buildings. Quantifying this influence requires the integration of OB models with building performance simulation (BPS). This study reviews approaches to representing and implementing OB models in today’s popular BPS programs, and discusses weaknesses and strengths of these approaches and key issues in integrating of OB models with BPS programs. Two of the key findings are: (1) a common data model is needed to standardize the representation of OB models, enabling their flexibility and exchange among BPS programs and user applications; the data model can be implemented using a standard syntax (e.g., in the form of XML schema), and (2) a modular software implementation of OB models, such as functional mock-up units for co-simulation, adopting the common data model, has advantages in providing a robust and interoperable integration with multiple BPS programs. Such common OB model representation and implementation approaches help standardize the input structures of OB models, enable collaborative development of a shared library of OB models, and allow for rapid and widespread integration of OB models with BPS programs to improve the simulation of occupant behavior and quantification of their impact on building performance.

32 ENERGY CONSERVATION, CONSUMPTION, AND UTILIZATI↗

Method to account for natural fracture induced elastic anisotropy in geomechanical characterization of shale gas reservoirs

Shale has been usually recognized as a transverse isotropic (TI) medium in conventional geomechanical log interpretation due to its laminated nature. However, when natural fractures exist in the shale rock, additional elastic anisotropy is introduced, converting laminated Shale to an orthorhombic (OB) medium. Previous studies illustrate that neglecting the natural fracture induced anisotropy in shale geomechanical log interpretation could lead to inaccurate evaluations of elastic moduli and in-situ stresses. In this paper, a new method is developed to account for the natural fracture induced anisotropy in geomechanical log interpretation based upon the TI acoustic model developed by the author and a characterization technique of elastic wave anisotropy (Sayers, 1991). The new OB model incorporates the four acoustic log data inputs and five modeling constraints in a nonlinear optimization algorithm to solve for the nine independent stiffness coefficients of an OB rock, and further to solve for the geomechanical properties and in-situ stress profiles in an OB formation. The new method was validated with a Marcellus Gas Shale field case. Both the new OB model and the conventional TI model were applied to interpret the minimum horizontal stress profile for the same formation. By comparing the results, the OB model is more robust than the TI from two aspects. First, the average stress magnitude predicted by the OB model is closer to the one measured by the Diagnostic Fracture Injection Test (DFIT). Second, the OB model predicts a more obvious stress barrier between the lower Marcellus and upper Onondaga Limestone than the TI model does. Finally, the predicted stress barrier is consistent with the observation of the microseismic events of a horizontal well drilled and completed nearby, which reveals that no hydraulic fracture propagates downward through the bottom boundary of Marcellus Shale into the underlying Onondaga Limestone.

03 NATURAL GAS↗

1α,25-Dihydroxyvitamin D3 increases implant osseointegration in diabetic mice partly through FoxO1 inactivation in osteoblasts

Highlights: • FoxO1 ablation in osteoblasts favors glucose homeostasis in diabetic mice. • 1,25(OH){sub 2}D{sub 3} regulates glucose and bone metabolism partly through inactivation of FoxO1. • FoxO1 inhibitor may be a novel approach to enhance implant osseointegration. Oral implant osseointegration is delayed by hyperglycemia-generated oxidative stress (OS). Forkhead transcription factor 1 (FoxO1) is known to be viewed as a sensor to OS since reactive oxide species like H{sub 2}O{sub 2} regulates its activity. We previously demonstrated that 1,25(OH){sub 2}D{sub 3} favored glucose homeostasis and implant osseointegration in diabetic rats. In this study, we investigated the role of FoxO1{sub OB} in the regulation process of 1,25(OH){sub 2}D{sub 3} on glycometabolism and bone metabolism. We show herein that, with the treatment of 1,25(OH){sub 2}D{sub 3}, mice lacking FoxO1 in osteoblasts (FoxO1{sub OB}{sup −/-}) exhibited decreased serum glucose that was gradually elevated in untreated diabetic mice. An optimal increase of bone mass and bone-implant contact (BIC) was observed in 1,25(OH){sub 2}D{sub 3} treated FoxO1{sub OB}{sup −/-} mice after 2-month healing. Surprisingly, FoxO1{sub OB}{sup −/-} mice without 1,25(OH){sub 2}D{sub 3} treatment also showed an improvement on bone formation and BIC. Same effect could be found in the expression of bone-related markers Runx2, Osterix and BSP, which elevated in 1,25(OH){sub 2}D{sub 3} treated FoxO1{sub OB}{sup −/-} mice as compared to untreated WT mice. In addition, in vitro study showed that high glucose induced FoxO1 nuclear localization while the effect was ameliorated by 1,25(OH){sub 2}D{sub 3} treatment. These results suggest that FoxO1{sub OB} might be involved in the regulation of 1,25(OH){sub 2}D{sub 3} on glucose homeostasis and bone formation, and that FoxO1{sub OB} might act as a key modulator of the capacity of the skeleton regulating metabolic homeostasis. Our study also provides a new idea that a combination of systemic 1,25(OH){sub 2}D{sub 3} and local FoxO1 inhibitor may be a new approach to enhance implant osseointegration.

60 APPLIED LIFE SCIENCES↗

Effect of dietary fiber fermentation on short‐chain fatty acid production and microbial composition in vitro

Abstract BACKGROUND The efficient utilization of fiber‐rich co‐products is important for optimizing feed resource utilization and animal health. This study was conducted to evaluate the fermentation characteristics of fiber‐rich co‐products, which had equal quantities of total dietary fiber (TDF), at different time points using batch in vitro methods. It considered their gas production, short‐chain fatty acid (SCFA) production, and microbial composition. RESULTS The fermentation of wheat bran (WB) and oat bran (OB) showed higher and faster ( P < 0.05) gas and SCFA production than corn bran (CB), sugar beet pulp (SBP), and soybean hulls (SH). The α ‐diversity was higher in the CB, SBP, and SH groups than in the WB and OB groups ( P < 0.05). At the phylum level, OB and WB fermentation showed lower ( P < 0.05) relative abundance of Actinobacteria than the CB, SBP, and SH groups. At the genus level, OB and WB fermentation increased the Enterococcus population in comparison with the CB, SBP, and SH groups, whereas CB and SBP fermentation improved the relative abundance of the Christensenellaceae R‐7 group more than the WB, OB, and SH groups ( P < 0.05). CONCLUSION Overall, WB and OB were rapidly fermented by fecal microbiota, in contrast with SBP, SH, and CB. Fermentation of different fiber‐rich co‐products with an equal TDF content gives different responses in terms of microbial composition and SCFA production due to variations in their physicochemical properties and molecular structure. © 2020 Society of Chemical Industry

Bai, Yu↗

Characterization of diterpene synthase genes in the wild rice species Oryza brachyatha provides evolutionary insight into rice phytoalexin biosynthesis

Highlights: • Diterpene-type phtyoalexin oryzalexins are only found in Oryza sativa. • Copalyl diphosphate synthase (CPS) and kaurene synthase-like in turn make diterpenes. • A distant wild rice species O. brachyantha has different CPS activity to O. sativa. • CPS activity type determines the range of diterpene-type phytoalexins produced. • syn-CPS acquisition was necessary for evolution of oryzalexin S production in rice. Cultivated rice (Oryza sativa; Os) produces a variety of labdane-related diterpenoids; not only phytohormone gibberellins (GAs) but also phytoalexins for defense including phytocassanes, momilactones and oryzalexins. Their carbon skeleton diterpenes are constructed from geranylgeranyl diphosphate via ent-copalyl diphosphate (ent-CDP) or its diastereomer syn-CDP. These two-step reactions are successively catalyzed by homologs of the two diterpene synthases, ent-CDP synthase (ent-CPS) and ent-kaurene synthase (KS) that are responsible for the biosynthesis of GAs; e.g. OsCPS4 and OsKSL8 that are involved in the biosynthesis of oryzalexin S, a rice phytoalexin. Oryza brachyantha (Ob) is the most distant wild rice species from Os among the Oryza genus. We previously reported that the Ob genome contains ObCPS{sub 1}1g, ObKSL8-a, ObKSL8-b and ObKSL8-c for specialized metabolism at a locus similar to the OsKSL8 locus on chromosome 11. These Ob genes are closely related to OsCPS4 and OsKSL8, respectively. We herein characterize the diterpene synthase genes in Ob, using functional analyses and expression analysis. Recombinant OsKSL8 and ObKSL8-a showed the same in vitro function when syn-CDP or normal-CDP were used as substrates. Nonetheless, our results suggest that Ob produces normal-CDP-related diterpenoid phytoalexins, presumably via ObKSL8-a, while Os produces a syn-CDP-related phytoalexin, oryzalexin S, via OsKSL8. This difference must be due to the kinds of CPS that are present in each species; Os has OsCPS4 encoding syn-CPS, while Ob has ObCPS{sub 1}1g encoding normal-CPS. Thus, we propose the evolutionary history underlying oryzalexin S biosynthesis: the gain of a syn-CPS was a critical event allowing the biosynthesis of oryzalexin S.

60 APPLIED LIFE SCIENCES↗

Alzheimer's disease and alpha-synuclein pathology in the olfactory bulbs of infants, children, teens and adults ≤ 40 years in Metropolitan Mexico City. APOE4 carriers at higher risk of suicide accelerate their olfactory bulb pathology

Highlights: • Air pollution targets olfactory bulbs in young city dwellers. • Olfactory bulb hyperphosphorylated tau and Lewy neurites are present in Mexico City toddlers. • APOE4 carriers accelerate their OB pathology and have 4.7 times higher suicide odds v APOE3. • Olfactory testing may aid in identifying young persons at high risk for neurodegenerative disease. • Early neuroprotective interventions in PM{sub 2.5} and CDNP's exposed individuals ≤ 20 years are critical. There is growing evidence that air pollution is a risk factor for a number of neurodegenerative diseases, most notably Alzheimer's (AD) and Parkinson's (PD). It is generally assumed that the pathology of these diseases arises only later in life and commonly begins within olfactory eloquent pathways prior to the onset of the classical clinical symptoms. The present study demonstrates that chronic exposure to high levels of air pollution results in AD- and PD-related pathology within the olfactory bulbs of children and relatively young adults ages 11 months to 40 years. The olfactory bulbs (OBs) of 179 residents of highly polluted Metropolitan Mexico City (MMC) were evaluated for AD- and alpha-synuclein-related pathology. Even in toddlers, hyperphosphorylated tau (hTau) and Lewy neurites (LN) were identified in the OBs. By the second decade, 84% of the bulbs exhibited hTau (48/57), 68% LNs and vascular amyloid (39/57) and 36% (21/57) diffuse amyloid plaques. OB active endothelial phagocytosis of red blood cell fragments containing combustion-derived nanoparticles (CDNPs) and the neurovascular unit damage were associated with myelinated and unmyelinated axonal damage. OB hTau neurites were associated mostly with pretangle stages 1a and 1b in subjects ≤ 20 years of age, strongly suggesting olfactory deficits could potentially be an early guide of AD pretangle subcortical and cortical hTau. APOE4 versus APOE3 carriers were 6–13 times more likely to exhibit OB vascular amyloid, neuronal amyloid accumulation, alpha-synuclein aggregates, hTau neurofibrillary tangles, and neurites. Remarkably, APOE4 carriers were 4.57 times more likely than non-carriers to die by suicide. The present findings, along with previous data that over a third of clinically healthy MMC teens and young adults exhibit low scores on an odor identification test, support the concept that olfactory testing may aid in identifying young people at high risk for neurodegenerative diseases. Moreover, results strongly support early neuroprotective interventions in fine particulate matter (PM{sub 2.5}) and CDNP's exposed individuals ≤ 20 years of age, and the critical need for air pollution control.

54 ENVIRONMENTAL SCIENCES↗

Adjusting solar-induced fluorescence to nadir-viewing provides a better proxy for GPP

Solar-induced fluorescence (SIF) provides key information for inferring terrestrial gross primary productivity (GPP). However, there is little research on analyzing the performance of nadir-adjusted SIF (SIF nadir ) on GPP, compared to total SIF emitted by all leaves (SIF total ) for reducing the viewing angle effects and estimating GPP. Besides, there have been controversial opinions on whether SIF total is better correlated to GPP than raw SIF observations (SIF obs ). Here we systematically analyzed the relationship between raw/nadir/total SIF (i.e., SIF obs , SIF nadir and SIF total ) and GPP and investigated the underlying mechanism, using multi-angular field measurements and eddy covariance data in wheat and corn crops at sub-daily scale. We further compared the performance of SIF obs , SIF nadir and SIF total in estimating GPP using the TROPOspheric Monitoring Instrument (TROPOMI) SIF and concurrent AmeriFlux measurements at daily scale. Furthermore, the results indicate that diurnal SIF nadir has stronger correlations to GPP than SIF obs for field measurements, with the increase of mean coefficient of determination (R 2 ) by 0.05–0.07 for far-red band and 0.11–0.20 for red band. SIF nadir shows comparable performance with SIF total for both far-red and red bands. Although the viewing angle effects dominate the difference in estimating GPP between SIF nadir and SIF obs , the correlation between light use efficiency (LUE) and fesc further determines the different performance of SIF nadir and SIF total in estimating GPP. TROPOMI-based analysis further confirms that SIF nadir overall has higher correlations to AmeriFlux GPP than SIF obs for different plant functional types and shows similar performance with SIF total . Compared to SIF total , the estimation of SIF nadir independent of viewing angle effects does not require any canopy structure parameters, and thus offers promising potential for reliably estimating regional and global GPP.

54 ENVIRONMENTAL SCIENCES↗

Generating high-resolution total canopy SIF emission from TROPOMI data: Algorithm and application

Solar-induced chlorophyll fluorescence (SIF) is a rapidly advancing front in modeling global terrestrial gross primary production (GPP). Canopy total SIF emissions (SIF total ) are mechanistically linked to the plant photosynthesis, and can be estimated from satellite observed SIF (SIF obs ) through radiative transfer modeling. However, the current satellite SIF obs and thus SIF total are available only at coarse spatial resolutions from several kilometers to tens of kilometers, inhibiting the application at fine spatial scales. Here, in this work, we proposed an algorithm to generate both global high-resolution SIF total (HSIF total ) and high-resolution SIF obs (HSIF obs ) at 1 km from low-resolution SIF obs (LSIF obs ) from the TROPOspheric Monitoring Instrument (TROPOMI), which has a spatial resolution at nadir of 3.5 km by 5.6–7 km. Our statistical method is based on the law of energy conservation and uses satellite derived fraction of absorbed photosynthetically active radiation, fluorescence efficiency, and the escape probability of fluorescence. We evaluated the accuracy of our HSIF total using the Orbiting Carbon Observatory-2 SIF (R 2 = 0.78). We found that the spatial resolution had clear effects on the relationship between HSIF total and GPP. We also compared HSIF total to 8-day averaged tower GPP from 135 flux sites and found that they were better correlated when HSIF total was averaged over a 1-km radius around the tower than when averaged over a larger radius. Our study provided a unique high-resolution HSIF total product, which will advance the estimation of GPP by extrapolating site-level relationships to the global scale.

54 ENVIRONMENTAL SCIENCES↗

Quantum mechanics and observers for gravity in a closed universe

Recent arguments based on the quantum extremal surface formula or the gravitational path integral have given fairly compelling evidence that the Hilbert space of quantum gravity in a closed universe is one-dimensional and real. How can this be consistent with the complexity of our own experiences? In this paper we propose that the experiences of any observer Ob in a closed universe can be approximately described by a quantum mechanical theory with a Hilbert space whose dimension is roughly e S Ob , where S Ob is the number of degrees of freedom of Ob. Moreover we argue that the errors in this description are exponentially small in S Ob . We give evidence for this proposal by incorporating it into the gravitational path integral and the coding interpretation of holography in simple models and seeing that it works, and we explain how similar effects arise in black hole physics in appropriate circumstances.

AdS-CFT Correspondence↗

A library of building occupant behaviour models represented in a standardised schema

During the past four decades, a significant body of literature has reported on the impacts of occupant behaviour (OB) on building technologies, operation, and energy consumption. A large number of data-driven behavioural models have been developed based on field data. These models lack standardisation and consistency, leading to difficulties in applications and comparison. To address this concern, an ontology was developed using the drivers-needs-actions-systems (DNAS) framework. Recent work has been carried out to implement the theoretical DNAS framework into an eXtensible Markup Language (XML) schema, titled ‘occupant behaviour XML’ (obXML) which is a practical implementation of OB models that can be integrated into building performance simulation (BPS) programs. This paper presents a newly developed library of OB models represented in the standardised obXML schema format. This library provides ready-to-use examples for BPS users to employ more accurate occupant representation in their energy models. The library, which contains an initial effort of 52 OB models, was made publicly available for the BPS community. As part of the library development process, limitations of the obXML schema were identified and addressed, and future improvements were proposed. Authors hope that by compiling this library building, energy modellers from all over the world can enhance their BPS models by integrating more accurate and robust OB patterns.

32 ENERGY CONSERVATION, CONSUMPTION, AND UTILIZATI↗

Enhancing occupant behavior representation for interoperability between building information modeling and building energy modeling

Building Performance Simulation (BPS) has been adopted as an essential tool for designing, operating, and retrofitting buildings to optimize energy efficiency throughout the building life cycle. The Green Building XML (gbXML) schema facilitates seamless data exchange between Building Information Modeling (BIM) and Building Energy Modeling (BEM) software tools. However, limited occupant behavior (OB) representation in BIM often leads to inconsistent and inaccurate energy simulation in BEM software. This paper presents 154 systematic enhancements to the existing occupant behavior XML (obXML) schema v1.3.4, initially developed for standardizing OB representation for BEM, to address existing limitations and improve interoperability with BIM models. The enhancements encompass improved integration with BIM models through extended building representations and system operations, expanded support for advanced OB models with additional environmental parameters and mathematical capabilities, and implementation of a standardized model documentation framework. To facilitate seamless data transformation between gbXML and obXML schemas, we developed a publicly available gb-obXML Schema Converter. Three case studies demonstrate the enhanced schema’s capabilities: representation of building information using a two-story office building model, documentation of a window operation behavior model, and validation of the schema converter’s functionality. The enhanced obXML schema v1.4 enables sophisticated modeling of occupant-building interactions while maintaining consistency with industry-standard BIM schemas. The standardized documentation framework facilitates reproducibility and knowledge sharing in the OB research community, while the schema converter automates the integration of building information into OB simulation workflows. These enhancements establish a foundation for more accurate building performance simulation by supporting sophisticated representation of occupant behavior within the BIM-to-BEM simulation workflows.

Chung, Jihoon↗

Structural and Stratigraphic Controls on Methane Hydrate occurrence and distribution: Gulf of Mexico, Walker Ridge 313 and Green Canyon 955: Final Report

The goal of this project was to determine structural and stratigraphic controls on hydrate occurrence and distribution in Green Canyon (GC) 955 and Walker Ridge (WR) 313 blocks using seismic and well data. Gas hydrate was discovered in these blocks in coarse- and fine-grained sediments during the 2009 Joint Industrial project (JIP) Leg 11 drilling expedition. Although the immediate interest of the exploration community is exclusively hydrate which is present in coarse–grained sediments, factors that control hydrate and free gas distribution in the two blocks and whether coarse and fine-grained hydrate-bearing units are related in any manner, formed the core of this research. The project spanned from 10/01/2012 to 07/31/2016. In the project, in both the leased blocks, the interval spanning the gas hydrate stability zone (GHSZ) was characterized using a joint analysis of sparse Ocean Bottom Seismic (OBS) and dense, surface–towed multichannel seismic (MCS) data. The project team had the luxury of calibrating their results with two well logs. Advance processing methods such as depth migration and full-waveform inversion (FWI) were used for seismic data analysis. Hydrate quantification was achieved through interpretation of the FWI velocity field using appropriate rock physics models at both blocks. The seismic modeling/inversion methodology (common to both GC955 and WR313 blocks) was as follows. First, the MCS data were depth migrated using a P-wave velocity (VP) model constructed using inversion of reflection arrival times of a few (four in both cases) key horizons carefully picked in the OBS data to farthest possible offsets. Then, the resolution of the traveltime VP model was improved to wavelength scale by inverting OBS gathers up to the highest frequency possible (21.75 Hz for GC955 and 17.5 for WR313) using FWI. Finally, the hydrate saturation (or the volume fraction) was estimated at the well location assuming one of the other hydrate morphology (filling the primary or the secondary porosity) was extrapolated out from the wells using the FWI VP as a guide. General outcomes were as follows. First and foremost, an imaging methodology using sparse seismic data, which is easily replicable at other sites with similar datasets, has been demonstrated. The end product of this methodology at both the leased blocks is quantitative estimates of hydrate distribution. Second, at both locations there is strong evidence that the base of the GHSZ, which does not appear as a clear Bottom Simulating Reflection (BSR), manifests in the VP perturbations created by FWI, suggesting that FWI is sensitive to subtle compositional changes in shallow sediments and establishes it as a valuable tool for investigations of hydrate-bearing basins. Third, through joint interpretation of the depth migrated image and the FWI VP model, how structure and stratigraphy jointly determine hydrate and free gas distribution in both blocks could be clearly visualized. The joint interpretation also suggests that the coarse and fine grained hydrate-bearing sediments at both leased are connected. Site specific results, in addition to general results, are as follows. At GC955 the overlying fine-grained hydrate-bearing unit could have been sourced from the underlying hydrate coarse-grained channel-levee complex through a chimney feature. The channel-levee system at GC955 is compartmentalized by faults, of which only a few may be impermeable. Although compartmentalized, the channel-levee system in the GC955 as a whole might be in communication except selected zones. At WR313 the overlying fine-grained fracture-filled hydrate unit appears to be sourced from below the GHSZ. The reason that only a particular fine-grained unit has hydrate, despite having lower porosity that the bounding units, could be the presence of secondary porosity (such as those formed from clay dewatering under compaction). In conclusion, the project was a pioneering effort in in joint analysis of OBS and MCS datasets for advancing the knowledge about a hydrate and free–gas system dynamics using advanced processing methods such as FWI and depth migration. Results obtained in this project can greatly advance the tools and techniques used for delineating specific hydrate prospects. Results obtained in this project can also be seamlessly incorporated into other DOE funded project on modeling the potential productivity and commercial viability of hydrate from sand-dominated reservoirs. The OBS and MCS data in this project were acquired in 2012 (after the JIP II drilling) by the USGS and therefore the results are a posteriori. Nonetheless, the seismic inversion workflow established through this project can be used to generate various what-if quantification scenarios even in absence of logs and serve as a valuable tool for guiding drilling operations. Results from this project can augment other DOE sponsored projects on determining the commercial viability of methane production from the Gulf of Mexico.

03 NATURAL GAS↗

On the applicability of various levels of detail for occupant behavior representation and modeling in building performance simulation

Occupant behavior (OB) is one of the significant sources of uncertainty in building performance simulation. While OB modeling has received increased attention in the past decade, research on the degree of granularity or level of detail (LoD) required for representing occupants is still in the nascent stages. This paper analyzes the modeling and applicability of three LoDs to represent occupants in building performance assessment. A medium-sized prototype office building located in Chicago, Illinois is used as the simulation case study. Ten occupant-centric attributes are adopted to develop the LoDs for OB representation. We first demonstrate the different modeling approaches required for simulating the three fidelity levels. Later, we illustrate the suitability of the developed LoDs in supporting six building performance use cases across different lifecycle stages. Furthermore, this study intends to provide guidance for the building simulation community on appropriate OB representation to support various use cases.

32 ENERGY CONSERVATION, CONSUMPTION, AND UTILIZATI↗