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Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

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The Effects of Placental-expanded (PLX-PAD) Stromal Cell Treatment, Hindlimb Unloading, and Isolation on the Behavior of Female Mice

Spaceflight can lead to altered immune responses and inflammation (Crucian et al. 2014) and elevated levels of inflammation are connected to anxiety and depression. A recent study on the International Space Station showed that mice exhibited a novel circling behavior during spaceflight (Ronca et al. 2019). However, there is still a gap in knowledge on how microgravity impacts behavior. In this current study, we performed 30 days of hindlimb unloading (HU) on four-month old female mice and analyzed select behavior from video image capture. We also determined the effects of PLacental-eXpanded stromal cells derived from the maternal placenta (PLX-PAD), alone and in combination with HU and isolation, on behavior. We have previously shown that PLX-PAD mitigates select inflammatory responses and changes in cytokine expression caused by HU. In-cage behaviors were analyzed in HU or control female mice treated with 2 injections of PlasmaLyte (Sham) or PLX-PAD (n=7/group). We found that PLX-PAD decreased exploratory behaviors compared to Sham-treated mice at night. Normally loaded (NL) PLX mice slept less than NL Sham mice during the day, and HU animals had significantly higher involuntary movement during sleep compared to NL animals, suggesting sleep disruption. Overall, we show that both HU and PLX affect important behavioral factors. This experiment is the first to study the effects of PLX-PAD on behavior. Additional studies are needed to define the behavioral changes in spaceflight and test possible countermeasures.

PLacental-EXpanded stromal cells, behavior, microg↗

The Effects of Simulated Microgravity and PLacental-EXpanded (PLX-PAD) Stromal Cell Treatment on The Behavior and Correlation with Cytokine Profiles in Female Mice

Spacelight can lead to altered immune responses and inflammation (Crucian et al. 2014) and elevated levels of inflammation are connected to anxiety and depression. A recent study on the International Space Station showed that mice exhibited a novel circling behavior during spaceflight (Ronca 2019). However, there is still a gap in knowledge on how microgravity impacts behavior. In this current study, we performed 30 days of hindlimb unloading (HU) on four-month old female mice and analyzed select behavior from video image capture. We also determined the effects of PLacental-eXpanded stromal cells dervided from the maternal placenta (PLX-PAD), alone and in combination with HU and isolation, on behavior. We have previoulst shown that PLX-PAD mitigated select inflammatory responses and changes in cytokine expression causes by HU. In-cage behaviors were analyzed in HU or control female mice treated with 2 injections of PlasmaLyte (Sham) or PLX-PAD (n=7/group). We found that PLX-PAD decreased exploratory behaviors compared to Sham treated mice at night. Normally loaded (NL) PLX mice slept less than NL Sham mice during the day, and HU animals had signficantly higher involuntary movement during sleep compared to NL animals, suggesting sleep distruption. Overall, we show that both HU and PLX affect important behavioral factors., This experiment is the first to study the effects of PLX-PAD on behavior. Additional studies are needed to define the behavioral changes in spaceflight and test possible countermeasures. Funding for this project was provided through the 2019 Ames Research Innovation Award (ARIA) provided by NASA Ames Research Center.

Stromal cell treatment↗

Multi-camera imaging to characterize jet and liner uniformity on the Plasma Liner Experiment (PLX)

At Plasma Liner Experiment, a set of 36 coaxial plasma guns are deployed quasi-uniformly over a 9 ft diameter spherical chamber and are used to form a high-Z spherically compressive plasma liner. Simulations indicate that for the concept to ultimately achieve optimal target density and temperature, a high degree of timing uniformity is required between all guns. Therefore, to aid in quantifying and correcting gun-to-gun nonuniformities, a key diagnostic will consist of up to six fisheye-view CCD cameras positioned inside the main chamber such that each has all plasma guns within its view. The individual cameras can be triggered at different times to determine each plasma jet’s muzzle velocity and structure for different operating conditions. This camera array is currently under development, and the implementation needs and challenges for this camera array are discussed here. Additionally, we detail the analysis methodology for determining jet-to-jet uniformity deviations and how we can correct them, thereby improving overall liner uniformity.

70 PLASMA PHYSICS AND FUSION TECHNOLOGY↗

Human placenta-derived stromal cells mitigate select immune responses to simulated weightlessness

Space flight presents unique risks to human health. Microgravity and social isolation are important space flight stressors which have vast impact on multiple systems such as the musculoskeletal, immune and central nervous system (CNS). To explore candidate countermeasures against tissue decrements caused by spaceflight, we performed a pilot study with PLacental-eXpanded stromal (PLX) cells. PLX cells secrete factors that modulate the immune system, but do not trigger host immune responses, enabling treatment in subjects that have intact immune systems. These cells had previous success in treating critical limb ischemia and acute radiation syndrome in murine models. In this study, 4-month-old female mice underwent 30 days of hindlimb unloading (HU) in single housing conditions to simulate long-term weightlessness and isolation. HU and normally ambulating controls were administered with PLX cells or sham-injected with Plasmalyte. We hypothesized that administration of PLX cells will mitigate some of the effects caused by prolonged HU. We found that treatment with PLX cells was well tolerated as indicated by body weights, circulating corticosterone levels and immune organ response to injection of the cells. HU led to the expected decreases in soleus muscle weights and bone loss. We did not observe any protective properties of the PLX cells in the musculoskeletal system but found that the cell treatment partially protected from a subset of HU-induced immune and CNS changes. In the hippocampus, protein levels of 9 of 44 cytokines were downregulated by HU. PLX administration mitigated changes in expression of 8 of these cytokines, including IL-2, IL-6 and MCP-1. To our knowledge, this is the first report on the protective effects of PLX-PAD against HU-induced changes in the cytokine milieu of the CNS. Our findings warrant further studies, which includes testing in actual spaceflight.

Microgravity↗

Plasma Guns for Magnetized Fuel Targets for PJMIF

In plasma jet driven magneto-inertial fusion (PJMIF) an array of discrete supersonic plasma jets is used to form a spherically imploding plasma liner, which then compresses a magnetized plasma target to fusion conditions. With funding from ARPA-E’s ALPHA program and from Strong Atomics LLC, HyperJet Fusion Corp and HyperV Technologies Corp. previously developed the plasma guns required for an experimental demonstration of the plasma liner formation part of the concept. A 36-gun demonstration of an imploding spherical plasma liner is currently underway on the PLX facility at Los Alamos National Laboratory. This present project addresses the next step required for a complete PJMIF concept, i.e. developing the magnetized plasma target. We proposed to form the target by stagnating a number of magnetized plasma jets in the center of the target chamber. This is accomplished by adding a bias field coil to the plasma liner gun to form a magnetized plasma jet. This experimental development took place at HyperJet in a geometry replicating the bias field environment that will be seen on a PLX port so the results are directly transferrable to the PLX experiment. The objective of this effort was the technical development and characterization of a new magnetized plasma jet using a high-performance, high momentum flux, contoured-gap coaxial plasma gun appropriate for use on the next stage of the PLX experiment. Electromagnetic modeling of the coil indicated the coil was best placed around the aluminum tubes of the gun transmission line, rather than around the chamber port as originally proposed. This maximized field strength in the breech and yielded much better flux linkage between gun electrodes. A 30-turn coil was ultimately implemented, allowing long pulses that could diffuse through the metal walls on the timescale of interest. \machtwo modeling predicted that less capacitance in the main PFN could potentially improve plasma jet velocities due to better matching of the current to the smaller plasma mass and the existing electrode contour designed to suppress blowby. This proved true, as testing showed markedly improved performance when the original 600uF bank was reduced to 400uF. A number of diagnostics were built and/or upgraded in order to characterize the plasma jets, inluding laser interferomtery for density, photodiodes for velocity, Bdot probes for magnetic field measurements, a Triple probe for temperature measurements, and spectroscopy for impurity content. A plasma gun with the 30-turn magnet coil installed, a 70% reduction in gas valve plenum volume, and a 33% reduction in main PFN capacitance produced a dense, high velocity, well magnetized plasma jet. We met or exceeded virtually all of the plasma jet parameter goals. Peak velocity of 135 km/s exceeded the 100 km/s goal by 35%, while the peak density of over 1.0x10 15 cm -3 was 3.3 times the goal of >3.0x10 14 cm -3 . Shot-to-shot repeatability is excellent, with a jitter of less than 300ns observed on the arrival fronts of the photodiode signals from one shot to the next. Average plasma jet lengths of 33cm (at 120km/s) were slightly longer than the 20cm goal, but jets as short as 11.8cm were observed at 135km/s. Mass is much higher than the targeted goal, averaging 106ug per shot compared to 20ug. The magnetic field is still a bit lower than desired, with a maximum to date of ~811G, compared to the goal of 1000G. Average values, though, were typically in the 300-450G range, when measured further downstream after some expected in flight decay. Temperature measurements are still a work in progress. Increasing the B field and completing temperature measurements will be continued on into the ongoing BETHE project.

70 PLASMA PHYSICS AND FUSION TECHNOLOGY↗

MTF Driven by Plasma Liner Dynamically Formed by the Merging of Plasma Jets: An Overview

One approach for standoff delivery of the momentum flux for compressing the target in MTF consists of using a spherical array of plasma jets to form a spherical plasma shell imploding towards the center of a magnetized plasma, a compact toroid (Figure 1). A 3-year experiment (PLX-1) to explore the physics of forming a 2-D plasma liner (shell) by merging plasma jets is described. An overview showing how this 3-year project (PLX-1) fits into the program plan at the national and international level for realizing MTF for energy and propulsion is discussed. Assuming that there will be a parallel program in demonstrating and establishing the underlying physics principles of MTF using whatever liner is appropriate (e.g. a solid liner) with a goal of demonstrating breakeven by 2010, the current research effort at NASA MSFC attempts to complement such a program by addressing the issues of practical embodiment of MTF for propulsion. Successful conclusion of PLX-1 will be followed by a Physics Feasibility Experiment (PLX-2) for the Plasma Liner Driven MTF.

Thio, Y. C. Francis↗

DOE SCGSR Final Report - Project Accomplishments and Additional Materials

The major accomplishments of my project were successfully developing and testing the laser interferometry diagnostic on the Plasma Liner Experiment (PLX) at Los Alamos National Laboratory (LANL). This diagnostic helped advance my research by providing a simpler way to obtain electron density measurements of magnetized plasmas compared to other electron density diagnostics such as triple Langmuir probes. The laser used was a 561 nm continuous wave (CW) diode-pumped solid state (DPSS) laser. The PLX laser interferometry diagnostic consists of two parts. These include the launching and receiving sides of the laser diagnostic. The launching side consists of the main laser beam from the DPSS laser being split into five chords(probe beams) and a reference beam which are then directed with fiber optic couplers into fiber optic cables which transmit the probe beamsto the PLX vacuum chamber. The probe beams then pass through the plasma in the chamber and into the receiving side optics where they are again directed through fiber optic cables to be combined with the reference beam. The combined beams are transmitted via multi-mode fiber optic cables to photodiodes to convert the light signals into electrical signals. Before the electrical signals get digitized, they pass through bandpass and low pass filters to eliminate electromagnetic noise and unwanted frequencies. The electrical signals are then processed by IQ demodulators to determine phase angle difference between the probe and reference beams for each chord in order to calculate line-integrated electron density.

70 PLASMA PHYSICS AND FUSION TECHNOLOGY↗

Particle-in-cell modeling of plasma jet merging in the large-Hall-parameter regime

The merging process of magnetized plasma jets with parameters relevant to the plasma-jet-driven magneto-inertial fusion (PJMIF) design and the plasma liner experiment (PLX) is modeled by fully kinetic particle-in-cell (PIC) simulations in one and two spatial dimensions. Here, the modified two-stream instability is identified to be the main mechanism responsible for stopping the plasma jets and preventing species interpenetration. The electron and ion Hall parameters of the merged plasma are greater than unity, and the plasma β is close to unity, which is the desired characteristic of planned experiments at PLX. Our 2D PIC simulations validate the results of the radiation magneto-hydrodynamics code FLASH, which will be the primary tool for modeling various stages of future PJMIF experiments.

70 PLASMA PHYSICS AND FUSION TECHNOLOGY↗

A Physics Exploratory Experiment on Plasma Liner Formation

Momentum flux for imploding a target plasma in magnetized target fusion (MTF) may be delivered by an array of plasma guns launching plasma jets that would merge to form an imploding plasma shell (liner). In this paper, we examine what would be a worthwhile experiment to do in order to explore the dynamics of merging plasma jets to form a plasma liner as a first step in establishing an experimental database for plasma-jets driven magnetized target fusion (PJETS-MTF). Using past experience in fusion energy research as a model, we envisage a four-phase program to advance the art of PJETS-MTF to fusion breakeven Q is approximately 1). The experiment (PLX (Plasma Liner Physics Exploratory Experiment)) described in this paper serves as Phase I of this four-phase program. The logic underlying the selection of the experimental parameters is presented. The experiment consists of using twelve plasma guns arranged in a circle, launching plasma jets towards the center of a vacuum chamber. The velocity of the plasma jets chosen is 200 km/s, and each jet is to carry a mass of 0.2 mg - 0.4 mg. A candidate plasma accelerator for launching these jets consists of a coaxial plasma gun of the Marshall type.

Thio, Y. C. Francis↗

Hollow Retroreflectors

A hollow retroreflector is a mirror-like instrument that reflects light and other radiations back to the source. After developing a hollow retroreflector for NASA's Apollo-Soyuz mission, PLX, Inc. continued to expand the technology and develop a variety of retroreflector systems. The Lateral Transfer Hollow Retroreflector maintains precise separation, at any wavelength, of incoming and existing beams regardless of their orientation. It can be used as an instrument or as a component of an optical system. In the laboratory, it offers a new efficient means of beam positioning. In other applications, it connects laser resonators, aligns telescope mirrors and is useful in general boresighting and alignment.

Source record↗

Flexible Peripheral Component Interconnect Input/Output Card

The Flexible Peripheral Component Interconnect (PCI) Input/Output (I/O) Card is an innovative circuit board that provides functionality to interface between a variety of devices. It supports user-defined interrupts for interface synchronization, tracks system faults and failures, and includes checksum and parity evaluation of interface data. The card supports up to 16 channels of high-speed, half-duplex, low-voltage digital signaling (LVDS) serial data, and can interface combinations of serial and parallel devices. Placement of a processor within the field programmable gate array (FPGA) controls an embedded application with links to host memory over its PCI bus. The FPGA also provides protocol stacking and quick digital signal processor (DSP) functions to improve host performance. Hardware timers, counters, state machines, and other glue logic support interface communications. The Flexible PCI I/O Card provides an interface for a variety of dissimilar computer systems, featuring direct memory access functionality. The card has the following attributes: 8/16/32-bit, 33-MHz PCI r2.2 compliance, Configurable for universal 3.3V/5V interface slots, PCI interface based on PLX Technology's PCI9056 ASIC, General-use 512K 16 SDRAM memory, General-use 1M 16 Flash memory, FPGA with 3K to 56K logical cells with embedded 27K to 198K bits RAM, I/O interface: 32-channel LVDS differential transceivers configured in eight, 4-bit banks; signaling rates to 200 MHz per channel, Common SCSI-3, 68-pin interface connector.

Bigelow, Kirk K.↗