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At least 19 records

Genome-wide Association Study Identifies Candidate Loci with Major Contributions to the Genetic Control of Pod Morphological Traits in Snap Bean

Snap beans are cultivars of common bean (Phaseolus vulgaris) that are cultivated for their fleshy immature pods that exhibit a wide diversity of pod shapes and sizes. The genetic basis of the snap bean pod shape is complex and involves the interaction of multiple genes. This study used a snap bean diversity panel composed of heirloom and improved cultivars used in North America and genome-wide association studies (GWAS) to investigate the genetic basis of pod morphological characteristics, including length, width, height, width/height ratio, and coefficients of variation (CVs). The GWAS detected multiple genomic regions associated with each pod trait, with a total of 20 quantitative trait loci (QTLs) for pod length, 9 for pod width, 14 for pod height, and 10 for pod width/height ratio. Regarding theCVof each pod trait, genome-wide association analyses detected six QTL for lengthCVs, five for widthCVs, 15 for heightCVs, and six for width/height ratioCVs. Thirteen regions in seven chromosomes were associated with two or more pod traits. Eighteen QTLs for pod traits in this study colocated with previously reported QTLs for pod and seed traits. The QTL intervals encompass gene models with homologues in other species that are involved in the control of developmental processes. These results capture the complex nature of the genetic control of snap bean pod traits and confirm the significance of genomic regions harboring overlapping QTLs identified in this and other studies. The phenotypic expression of pod traits in snap bean appears to be under the control of a few genomic regions with a strong effect with additional contributions of multiple small-effect regions. Validation of the function of the candidate genes identified in associated regions will contribute to our understanding of legume pod development.

Agriculture↗

Aerodynamic effects of five lift-fan pod arrangements on an unpowered V/STOL transport model

An investigation was conducted in the Langley V/STOL tunnel to determine the effect of longitudinally oriented wing-mounted pods on the longitudinal and lateral aerodynamic characteristics in the cruise flight condition of a high-wing V/STOL transport model. Five pod arrangements were tested - three configurations with in-line pods at 20, 40, or 60 percent semispan and two split pod configurations with rear pods at 20 percent semispan and front pods at 40 or 60 percent semispan. In general, addition of the pods to the model decreased the stability, increased the lift-curve slope, and alleviated the abrupt stall of the basic model. The configuration with pods at 20 percent semispan had an abrupt instability at 10 deg angle of attack, All the configurations had lateral stability at sideslip angles from 5 to -5 deg. Very little difference in results existed between the configurations with pods at 40 and 60 percent semispan. Of the split pod configurations, the configuration with front pods at 40 percent semispan offered the best trimmed lift and lift-induced drag characteristics at high angles of attack. The configuration with in-line pods at 40 or 60 percent semispan provided the best cruise characteristics of all the pod configurations.

Thomas, J. L.↗

Preliminary Orbit Determination System (PODS) for Tracking and Data Relay Satellite System (TDRSS)-tracked target Spacecraft using the homotopy continuation method

The Preliminary Orbit Determination System (PODS) provides early orbit determination capability in the Trajectory Computation and Orbital Products System (TCOPS) for a Tracking and Data Relay Satellite System (TDRSS)-tracked spacecraft. PODS computes a set of orbit states from an a priori estimate and six tracking measurements, consisting of any combination of TDRSS range and Doppler tracking measurements. PODS uses the homotopy continuation method to solve a set of nonlinear equations, and it is particularly effective for the case when the a priori estimate is not well known. Since range and Doppler measurements produce multiple states in PODS, a screening technique selects the desired state. PODS is executed in the TCOPS environment and can directly access all operational data sets. At the completion of the preliminary orbit determination, the PODS-generated state, along with additional tracking measurements, can be directly input to the differential correction (DC) process to generate an improved state. To validate the computational and operational capabilities of PODS, tests were performed using simulated TDRSS tracking measurements for the Cosmic Background Explorer (COBE) satellite and using real TDRSS measurements for the Earth Radiation Budget Satellite (ERBS) and the Solar Mesosphere Explorer (SME) spacecraft. The effects of various measurement combinations, varying arc lengths, and levels of degradation of the a priori state vector on the PODS solutions were considered.

Kirschner, S. M.↗

Open-Source Conceptual Sizing Models for the Hyperloop Passenger Pod

Hyperloop is a new mode of transportation proposed as an alternative to California's high speed rail project, with the intended benefits of higher performance at lower overall costs. It consists of a passenger pod traveling through a tube under a light vacuum and suspended on air bearings. The pod travels up to transonic speeds resulting in a 35 minute travel time between the intended route from Los Angeles and San Francisco. Of the two variants outlined, the smaller system includes a 1.1 meter tall passenger capsule traveling through a 2.2 meter tube at 700 miles per hour. The passenger pod features water-based heat exchangers as well as an on-board compression system that reduces the aerodynamic drag as it moves through the tube. Although the original proposal looks very promising, it assumes that tube and pod dimensions are independently sizable without fully acknowledging the constraints of the compressor system on the pod geometry. This work focuses on the aerodynamic and thermodynamic interactions between the two largest systems; the tube and the pod. Using open-source toolsets, a new sizing method is developed based on one-dimensional thermodynamic relationships that accounts for the strong interactions between these sub-systems. These additional considerations require a tube nearly twice the size originally considered and limit the maximum pod travel speed to about 620 miles per hour. Although the results indicate that Hyperloop will need to be larger and slightly slower than originally intended, the estimated travel time only increases by approximately five minutes, so the overall performance is not dramatically affected. In addition, the proposed on-board heat exchanger is not an ideal solution to achieve reasonable equilibrium air temperatures within the tube. Removal of this subsystem represents a potential reduction in weight, energy requirements and complexity of the pod. In light of these finding, the core concept still remains a compelling possibility, although additional engineering and economic analyses are markedly necessary before a more complete design can be developed.

design analysis↗

Design and test of a modified passive orbital disconnect strut (PODS-IV)

The design and testing of a modification to the Passive Orbital Disconnect Struts (PODS) III system (designed to reduce support conductance and superfluid-helium-tank dewar weights), PODS IV, are discussed. The PODS IV design was found to achieve a nominal increase of 630-812 percent in side-load resistance over the PODS-III design, with a predicted 15-percent increase in thermal conductance. It is noted that the increase in side-load resistance largely removes the limitation on length of the orbit tube inherent in the PODS III design. The higher resistance of the longer orbit tube results in lower total conductance for the PODS-IV design.

Spradley, Iran E.↗

Cacao pod transcriptome profiling of seven genotypes identifies features associated with post-penetration resistance to Phytophthora palmivora

Abstract The oomycete Phytophthora palmivora infects the fruit of cacao trees ( Theobroma cacao ) causing black pod rot and reducing yields. Cacao genotypes vary in their resistance levels to P. palmivora , yet our understanding of how cacao fruit respond to the pathogen at the molecular level during disease establishment is limited. To address this issue, disease development and RNA-Seq studies were conducted on pods of seven cacao genotypes (ICS1, WFT, Gu133, Spa9, CCN51, Sca6 and Pound7) to better understand their reactions to the post-penetration stage of P. palmivora infection. The pod tissue- P. palmivora pathogen assay resulted in the genotypes being classified as susceptible (ICS1, WFT, Gu133 and Spa9) or resistant (CCN51, Sca6 and Pound7). The number of differentially expressed genes (DEGs) ranged from 1625 to 6957 depending on genotype. A custom gene correlation approach identified 34 correlation groups. De novo motif analysis was conducted on upstream promoter sequences of differentially expressed genes, identifying 76 novel motifs, 31 of which were over-represented in the upstream sequences of correlation groups and associated with gene ontology terms related to oxidative stress response, defense against fungal pathogens, general metabolism and cell function. Genes in one correlation group (Group 6) were strongly induced in all genotypes and enriched in genes annotated with defense-responsive terms. Expression pattern profiling revealed that genes in Group 6 were induced to higher levels in the resistant genotypes. An additional analysis allowed the identification of 17 candidate cis -regulatory modules likely to be involved in cacao defense against P. palmivora . This study is a comprehensive exploration of the cacao pod transcriptional response to P. palmivora spread after infection. We identified cacao genes, promoter motifs, and promoter motif combinations associated with post-penetration resistance to P. palmivora in cacao pods and provide this information as a resource to support future and ongoing efforts to breed P. palmivora -resistant cacao.

60 APPLIED LIFE SCIENCES↗

Test and evaluate passive orbital disconnect struts (PODS 3)

The objectives of the Passive Orbital Disconnect Struts (PODS) test are to evaluate modal resonance of the PODS-III supports to obtain engineering data required for use of PODS-III on flight systems; determine possible performance improvements in large LO2/LH2 space applications. (1) Modal Vibration Tests. A modal resonance survey is performed on a set of six PODS-III struts assembled in a dewar simulator. The survey conditions simulate both launch and orbital loadings of the struts. The orbital load range spans a full to an empty tank. The frequencies surveyed cover the range consistent with Shuttle qualification requirements and the principal resonant modes of the strut system. (2) Benefit study. The benefit of using PODS-III supports on OTV and Space Station LO sub 2 and LH sub 2 reference tanks was compared to nondisconnect supports. Four LO sub 2 and LH sub 2 tanks were studied under various conditions: (1) holding the launch resonance at 35 Hz and varying the orbit resonance; (2) analyzing both full and emtpy tanks at launch; (3) varying orbit boundary temperaure; (4) varying the number of struts; (5) varying orbit times; and (6) using or not using vapor cooling.

Parmley, R. T.↗

Modeling the X-Ray Process, and X-Ray Flaw Size Parameter for POD Studies

Nondestructive evaluation (NDE) method reliability can be determined by a statistical flaw detection study called probability of detection (POD) study. In many instances the NDE flaw detectability is given as a flaw size such as crack length. The flaw is either a crack or behaving like a crack in terms of affecting the structural integrity of the material. An alternate approach is to use a more complex flaw size parameter. The X-ray flaw size parameter, given here, takes into account many setup and geometric factors. The flaw size parameter relates to X-ray image contrast and is intended to have a monotonic correlation with the POD. Some factors such as set-up parameters including X-ray energy, exposure, detector sensitivity, and material type that are not accounted for in the flaw size parameter may be accounted for in the technique calibration and controlled to meet certain quality requirements. The proposed flaw size parameter and the computer application described here give an alternate approach to conduct the POD studies. Results of the POD study can be applied to reliably detect small flaws through better assessment of effect of interaction between various geometric parameters on the flaw detectability. Moreover, a contrast simulation algorithm for a simple part-source-detector geometry using calibration data is also provided for the POD estimation.

Khoshti, Ajay↗

Modeling the X-ray Process, and X-ray Flaw Size Parameter for POD Studies

Nondestructive evaluation (NDE) method reliability can be determined by a statistical flaw detection study called probability of detection (POD) study. In many instances, the NDE flaw detectability is given as a flaw size such as crack length. The flaw is either a crack or behaving like a crack in terms of affecting the structural integrity of the material. An alternate approach is to use a more complex flaw size parameter. The X-ray flaw size parameter, given here, takes into account many setup and geometric factors. The flaw size parameter relates to X-ray image contrast and is intended to have a monotonic correlation with the POD. Some factors such as set-up parameters, including X-ray energy, exposure, detector sensitivity, and material type that are not accounted for in the flaw size parameter may be accounted for in the technique calibration and controlled to meet certain quality requirements. The proposed flaw size parameter and the computer application described here give an alternate approach to conduct the POD studies. Results of the POD study can be applied to reliably detect small flaws through better assessment of effect of interaction between various geometric parameters on the flaw detectability. Moreover, a contrast simulation algorithm for a simple part-source-detector geometry using calibration data is also provided for the POD estimation.

Koshti, Ajay M.↗

The Backscattering Contribution of Soybean Pods at L-band

L-band (1.25 GHz) radar measurements of a soybean canopy indicate that the emergence of seed pods is a significant contributor to the backscatter during the late stages of the growing season. In order to validate the measured data, a realistic scattering model of the soybean canopy is developed. The parameters of the soybean canopy and underlying soil used in the model vary over the growing season based on in situ measurements. Scattering amplitudes for soybean leaves are modeled analytically by using a thin disk approximation; stem and pods are jointly modeled using a numerical electromagnetic field solver. These scattering amplitudes are together incorporated into a coherent scattering model to obtain the backscattering coefficient for VV- and HH-polarizations. The modeling results show good agreement with the radar field measurements, having RMSEs of 0.51 dB for VV-pol and 1.1 dB for HH-pol. Both measured data and modeled results show that the change of soil moisture can be accurately monitored by L-band backscatter. It is also found that the difference between HH- and VV-polarized backscatter increases as the size of the soybean pods becomes larger. A method is developed here to estimate the number of pods in a soybean canopy based on polarimetric radar backscatter at L-band.

Radar backscatter↗

Sensitivity to Snapshot Frequency in the POD-Based Reduced-Order Modeling of Flow over a Gaussian Bump

The flow over a Gaussian bump at a Reynolds number of 10^6, based on the bump length and freestream conditions, and a Mach number of 0.2, was simulated using wall-modeled large eddy simulation (WMLES). The incoming turbulent flow exhibits relaminarization upstream of the bump apex, due to the favourable pressure gradient acceleration induced by the bump curvature, as well as separation, reattachment and a detached shear layer downstream of the apex. A Galerkin reduced-order model (ROM) was then constructed using a proper orthogonal decomposition (POD) modal basis. The complex, three-dimensional flow features are captured by spatial POD modes differently based on the frequency of the high-fidelity snapshots originally provided. POD modes constructed from differently sampled snapshot matrices were then truncated to retain 90% of the turbulence kinetic energy in the domain of interest. A parametric study is conducted to evaluate the effect of the frequency of snapshots used to construct the POD-ROM.

turbulence↗

A monkey metabolism pod for space-flight weightlessness studies.

The system described will permit quantitative physiological studies in adult monkeys, weighing from 8 to 14 kg, during future space flights. The system comprises a fiberglass pod containing a comfortable restraint couch for the animal. The pod is divided into upper and lower halves. When the monkey occupies the couch, a rubber belly-band forms a gas seal between the upper and lower portions of the animal. The upper-pod ventilating air stream is monitored for the partial pressures of oxygen, carbon dioxide, nitrogen, and water to permit continuous metabolic gas-exchange measurements for computation of metabolic energy expediture. The lower pod is lined with ashless filter paper for excreta collection.

Pace, N.↗

Results from the EPL monkey-pod experiment conducted as part of the 1974 NASA/Ames shuttle CVT-2

The participation of the Environmental Physiology Laboratory (EPL) in the general purpose laboratory concept verification test 3 is documented. The EPL Monkey-Pod Experiment was designed to incorporate a 10-12 kg, pig tailed monkey, Macaca nemestrina, into the pod and measure the physiological responses of the animal continuously. Four major elements comprise the EPL Monkey-Pod Experiment System: (1) a fiberglass pod containing the instrumented monkey plus feeder and watering devices, (2) an inner console containing the SKYLAB mass spectrometer with its associated valving and electronic controls, sensing, control and monitoring units for lower body negative pressure, feeder activity, waterer activity, temperatures, and gas metabolism calibration, (3) an umbilical complex comprising gas flow lines and electrical cabling between the inner and outer console and (4) an outer console in principle representing the experiment support to be provided from general spacecraft sources.

Rahlmann, D. F.↗

Results from the EPL monkey-pod flight experiments conducted aboard the NASA/Ames CV-990, May 1976

The participation of the Environmental Physiology Laboratory (EPL) in the general purpose laboratory concept verification test 3 is documented. The EPL Monkey-Pod Experiment was designed to incorporate a 10-12 kg, pig tailed monkey, Macaca nemestrina, into the pod and measure the physiological responses of the animal continously. Four major elements comprise the EPL Monkey-Pod Experiment System: (1) a fiberglass pod containing the instrumented monkey plus feeder and watering devices, (2) an inner console containing the SKYLAB mass spectrometer with its associated valving and electronic controls, sensing, control and monitoring units for lower body negative pressure, feeder activity, waterer activity, temperatures, and gas metabolism calibration, (3) an umbilical complex comprising gas flow lines and electrical cabling between the inner and outer console and (4) an outer console in principle representing the experiment support to be provided from general space craft sources.

Rahlmann, D. F.↗

Effects of a military cargo pod and tail fins on the aerodynamic characteristics of a large wide-body transport model

Wind tunnel tests were conducted on a 0.03 scale model of a large wide-body commercial aircraft to determine the effects on the static aerodynamic characteristics resulting from the attachment of a belly pod for the long-range deployment of outsize military equipment. The effectiveness of horizontal-tip fins in augmenting directional stability was investigated. At a test Reynolds number of 1.08 x 1,000,000, the addition of the pod results in an increase in total drag of approximately 20 percent. Trim drag due to the pod is very small. Although the pod produces a significant decrease in directional stability, the addition of the tip fins restores some of the stability, particularly at the lower angles of attack.

Jernell, L. S.↗

Hypersonic shock tunnel heat transfer tests of the Space Shuttle SILTS pod configuration

Heat transfer measurements have been made on a 0.0175-scale NASA Space Shuttle orbiter model having a simulated SILTS (Shuttle Infrared Leeside Temperature Sensor) pod on top of the vertical tail. Heat transfer distributions were measured both on the pod and on the vertical tail. The test program covered Mach numbers of 8, 11 and 16 in air, at Reynolds numbers from 100,000 to 18 million, based on model length. The angle of attack ranged from 30 deg to 40 deg at sideslip angles from -2 to +2 deg. Data were obtained with 92 thin film assistance thermometers located on the SILTS pod and on the upper 30 percent of the vertical tail. Heat transfer rates measured on the vertical tail show good agreement with flight data obtained from missions STS-1, -2 and -3. The variation of heat transfer to the pod with Reynolds number, Mach number and angle of attack is discussed.

Wittliff, C. E.↗

Probability of Detection (POD) Demonstration Transferability: Phase II

NASA Special Level POD demonstration tests are typically performed on flat plates of a single material containing fatigue cracks with aspect rations between 0.3 and 0.5. In many cases, the inspectors that pass the demonstration tests use (transfer) the NDE technique to inspect different materials and part geometries and for varying types of flaws. The objective of the task is to investigate several of the factors that may influence the transferability of POD demonstration tests. An existing set of 30 6061-T6 aluminum crack panels will be used to create parts with a square tube and pocket type geometries. These same aluminum crack panels were used in a study of the effect of penetrant sensitivity level on POD. Hence, we should be able to directly compare the POD for flat panels versus larger parts with more complex geometries.

Parker, Bradford H.↗

Global Ionospheric Electron Density from GNSS-POD Limb Measurements

GNSS-LEO radio links from Precise Orbital Determination (POD) and Radio Occultation (RO) antennas have been used increasingly in studying and monitoring the global ionospheric electron density (Ne). In this study we developed an optimal estimation (OE) method to retrieve Ne profiles from the slant total electron content (hTEC) measurements acquired by the GNSS-POD links at negative elevation angles. The hmF2 and NmF2 from the OE retrieval are validated against ground-based ionosondes and radar observations, showing generally good agreements in NmF2 from all sites. Nighttime hmF2 measurements tend to agree better than the daytime when the ionosonde heights tend to be slightly lower. The OE algorithm has been applied to all GNSS-POD data acquired from the COSMIC-1 (2006-2019), COSMIC-2 (2019-present), and Spire (2019-present) constellations, showing a consistent ionospheric Ne morphology. A detailed analysis of the frequency-wavenumber spectra is made for the Ne variability at different heights. In the lower ionosphere (~150 km) we found significant spectral power in DE1, DW6, DW4, SW5, and SE4 wave components, in addition to well-known DW1, SW2 and DE3 waves. In the upper ionosphere (~450 km), additional wave components are still present, including DE4, DW4, DW6, SE4 and SW4. The co-exist of eastward and westward propagating WN4 components implies the presence of a stationary planetary wave4 (sPW4), as suggested by other earlier studies.

GNSS-POD↗