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Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

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At least 19 records

Linking crop traits to transcriptome differences in a progeny population of tetraploid potato

Background: Potato is the third most consumed crop in the world. Breeding for traits such as yield, product quality and pathogen resistance are main priorities. Identifying molecular signatures of these and other important traits is important in future breeding efforts. In this study, a progeny population from a cross between a breeding line, SW93–1015, and a cultivar, Désirée, was studied by trait analysis and RNA-seq in order to develop understanding of segregating traits at the molecular level and identify transcripts with expressional correlation to these traits. Transcript markers with predictive value for field performance applicable under controlled environments would be of great value for plant breeding.Results: A total of 34 progeny lines from SW93–1015 and Désirée were phenotyped for 17 different traits in a field in Nordic climate conditions and controlled climate settings. A master transcriptome was constructed with all 34 progeny lines and the parents through a de novo assembly of RNA-seq reads. Gene expression data obtained in a controlled environment from the 34 lines was correlated to traits by different similarity indices, including Pearson and Spearman, as well as DUO, which calculates the co-occurrence between high and low values for gene expression and trait. Our study linked transcripts to traits such as yield, growth rate, high laying tubers, late and tuber blight, tuber greening and early flowering. We found several transcripts associated to late blight resistance and transcripts encoding receptors were associated to Dickeya solani susceptibility. Transcript levels of a UBX-domain protein was negatively associated to yield and a GLABRA2 expression modulator was negatively associated to growth rate.Conclusion: In our study, we identify 100’s of transcripts, putatively linked based on expression with 17 traits of potato, representing both well-known and novel associations. This approach can be used to link the transcriptome to traits. We explore the possibility of associating the level of transcript expression from controlled, optimal environments to traits in a progeny population with different methods introducing the application of DUO for the first time on transcriptome data. We verify the expression pattern for five of the putative transcript markers in another progeny population.

59 BASIC BIOLOGICAL SCIENCES↗

Actinide in Air (Rn-Progeny Rejected) Alpha Spectroscopy With Tensioned Metastable Fluid Detectors

Here, this article discusses outcome of research for deriving a methodology and apparatus for ascertaining for the presence of ultratrace-level actinides in air from their alpha emission signatures, while remaining blind to the relatively large (1000× higher activity) alpha emissions from Rn-progeny. Apparatus and techniques were developed to collect and characterize alpha-emitting nuclides of Rn-progeny and actinides in air on a polycarbonate 3 μm pore size continuous air monitor (CAM) filter. A wet-chemistry approach was developed and validated for successfully separating the Rn-progeny alpha emitting isotopes of Po-214 and Po-218, while extracting the actinides (U, Pu, Am) in a fluid mixture that is suitable for conduct of alpha spectroscopy with a centrifugally tensioned metastable fluid detector (CTMFD). The resulting α-TMFD technology was compared against the state-of-art “Alpha-Sentry™” Continuous Air Monitor (CAM) system commonly utilized world-wide. Results indicate that the α-TMFD technology can potentially offer complementary and superior performance in multiple performance categories, and ∼18× improvement in the time to detect (e.g., at 0.02 derived air concentration (DAC) within ∼3 h, versus ∼70 h for Alpha-Sentry) for actinides of interest while also remaining ∼100% blind to ∼10 3 × higher Rn-progeny background—with the added potential for offering few keV scale energy resolution without resorting to peak shape fitting, versus ∼300–400 keV for existing CAM systems.

46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND ↗

Characterization of Precipitation-Induced Radon Progeny Deposition Events Using a City-Scale Sensor Network

Networks of radiation detectors provide a platform for real-time radioactive source detection and identification in urban environments. Detection algorithms in these systems must adapt to naturally-occurring changes in background, which requires well-characterized relationships between precipitation events and their corresponding radiological signature. Here, we present a quantitative and qualitative description of rain-induced radon progeny deposition events occurring in Chicago from September 2023 to February 2024. We measure ambient gamma radiation levels, precipitation rate, temperature, pressure, and relative humidity in a network of sensor nodes. For each identified precipitation period, we decompose spectra into static- and radon-associated components as defined by a non-negative matrix factorization (NMF) algorithm. We find a consistent power-law relationship between a precipitation-dependent peak of the radon progeny proxy (RPP) and the peak strength of the radon-associated NMF component for most precipitation events. We conduct a case study of a rainfall period with abnormally high levels of implied radon progeny concentration and describe its temporal and spatial evolution. We hypothesize that this phenomenon is due to the air mass path that intersects a uranium-rich region of Wyoming. Finally, we cluster precipitation events into three distinct categories. One category roughly corresponds to events with deep low-pressure systems and high relative radon concentration, while another is characteristic of light stratiform rain with slightly higher temperatures and intermediate relative radon concentration. The third category appears to contain weak-gradient or lake breeze convection showers with intermittent precipitation and low relative radon concentration. These findings suggest that radiological anomaly detection could be improved by training unique background models corresponding to each category of meteorological event.

46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND ↗

Stochastic journeys of cell progenies through compartments and the role of self-renewal, symmetric and asymmetric division

Abstract Division and differentiation events by which cell populations with specific functions are generated often take place as part of a developmental programme, which can be represented by a sequence of compartments. A compartment is the set of cells with common characteristics; sharing, for instance, a spatial location or a phenotype. Differentiation events are transitions from one compartment to the next. Cells may also die or divide. We consider three different types of division events: (i) where both daughter cells inherit the mother’s phenotype (self-renewal), (ii) where only one of the daughters changes phenotype (asymmetric division), and (iii) where both daughters change phenotype (symmetric division). The self-renewal probability in each compartment determines whether the progeny of a single cell, moving through the sequence of compartments, is finite or grows without bound. We analyse the progeny stochastic dynamics with probability generating functions. In the case of self-renewal, by following one of the daughters after any division event, we may construct lifelines containing only one cell at any time. We analyse the number of divisions along such lines, and the compartment where lines terminate with a death event. Analysis and numerical simulations are applied to a five-compartment model of the gradual differentiation of hematopoietic stem cells and to a model of thymocyte development: from pre-double positive to single positive (SP) cells with a bifurcation to either SP4 or SP8 in the last compartment of the sequence.

Science & Technology - Other Topics↗

Comprehensive study of radon progeny attachment to surfaces

Low energy, low rate experiments, such as searches for neutrinoless double β decay and dark matter, require unprecedentedly low levels of background in order to deliver their full science potential. 210 Po driven, neutron induced background, caused by nuclear (α, n) reactions on low-Z materials, direct background contributions of the 210 Po α radiation and desorption of the 210 Pb progeny 210 Bi from surfaces into the detector medium are of particular of concern. These backgrounds depend on details of the components’ exposure to radon-loaded laboratory air and, thus, their handling history. The attachment rates of airborne radon progeny to surfaces, needed for the estimation of these background rates, are poorly understood. This article reports the results of a campaign comprising of more than 1200 attachment measurements, performed for nine different materials. Correlations of the attachment with environmental parameters such as air exchange rate, electrical surface potential, temperature, atmospheric pressure, and relative humidity have been studied and found to be significant only in case of the first two. Attachment modeling, using the Jacobi model, is compared to data.

73 NUCLEAR PHYSICS AND RADIATION PHYSICS↗

Air Exchange Rate Impact on Activity Equilibrium Factors and Inhalation Fractional Equilibrium Factors for Rn, Xe, Kr, Ar, Ne, and Their Progeny in Vapor Intrusion, Risk, and Dose Models

Exposure to the radioactive noble gasses, especially radon, is of high concern and poses a significant risk to humans in an indoor air environment as the second leading cause of lung cancer in the United States. To evaluate and minimize the risks posed by these gasses, it is important to understand their radiological and physical properties. The EPA’s Radon Vapor Intrusion Screening Level (RVISL) calculator calculates indoor air RVISLs based on target working levels (WLs), target excess lifetime cancer risk (ELCR), and annual dose limits for the actinon (Rn-219), thoron (Rn-220), and radon (Rn-222) decay series. The RVISLs are based on inhalation and submersion in gas cloud exposure routes for residential and commercial settings. The RVISLs are analogous to preliminary remediation goals (PRGs) and dose compliance concentrations (DCCs), where the isotope-specific values are in units of activity concentration (activity per unit volume). If the concentration of a parent isotope of radon or its progeny is found to exceed the RVISL, then further action to ensure cleanup of the contaminant may be necessary. In residential and commercial settings, the RVISLs will vary based on the air exchange rate present. The EPA’s Radionuclide PRG and DCC Calculators also assess the risk/dose from noble gases in the air due to household use of water like showering. In this study, a computational method in MATLAB was developed to determine the impact of the air exchange rate on the activity equilibrium factor (A eq ) and the inhalation fractional equilibrium factor (F eq ). Both factors are values that reflect the equilibrium concentrations of progeny to their parent in the air. These factors have a direct impact on the RVISL, PRG, and DCC calculations of WL, ELCR, and annual dose, respectively. This study builds on a previous report that only focused on actinon, thoron, and radon by revisiting the original A eq and F eq calculation methods, as well as including the values for the Rn-207, Rn-209, Rn-210, Rn-211, Rn-215, Rn-216, Rn-217, Rn-218, Rn-223, Ne-24, Ar-42, Ar-43, Ar-44, Kr-74, Kr-75, Kr-76, Kr-77, Kr-88, Kr-89, Xe-120, Xe-121, Xe-122, Xe-123, Xe-135m, and Xe-138 decay chains, which are not currently available in literature. The EPA’s RVISL calculator will be updated to include the new A eq and F eq values for the actinon, thoron, and radon decay chains, while the rest of the calculators will incorporate all the new A eq and F eq values as appropriate.

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTAN↗

Measurement of ambient radon progeny decay rates and energy spectra in liquid argon using the MicroBooNE detector

We report measurements of radon progeny in liquid argon within the MicroBooNE time projection chamber (LArTPC). The presence of specific radon daughters in MicroBooNE’s 85 metric tons of active liquid argon bulk is probed with newly developed charge-based low-energy reconstruction tools and analysis techniques to detect correlated Bi 214 − Po 214 radioactive decays. Special datasets taken during periods of active radon doping enable new demonstrations of the calorimetric capabilities of single-phase neutrino LArTPCs for β and α particles with electron-equivalent energies ranging from 0.1 to 3.0 MeV. By applying Bi 214 − Po 214 detection algorithms to data recorded over a 46-day period, no statistically significant presence of radioactive Bi 214 is detected, and a limit on the activity is placed at < 0.35 mBq / kg at the 95% confidence level. This bulk Bi 214 radiopurity limit—the first ever reported for a liquid argon detector incorporating liquid-phase purification—is then further discussed in relation to the targeted upper limit of 1 mBq / kg on bulk Rn 222 activity for the DUNE neutrino detector. Published by the American Physical Society 2024

46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND ↗

Removal of radon progeny from delicate surfaces

210 Po α-decay-driven neutron background is a concern for many rare event search experiments. It is a difficult to control background because its radiogenic component depends on the air exposure history of parts. In this study, we demonstrate that about half of the radon progeny 210 Po can be removed from copper and silicon surfaces relatively easily by wiping a copper sample with acetone-wetted tissue and a silicon detector with acetone soaked cotton balls. For a copper sample, we demonstrate that long-lived 210 Pb is removed with similar effectiveness. For copper, allocated the longest counting time, additional wiping was found to be largely ineffective. For silicon, the removal effectiveness has large uncertainties. Additional cleaning showed a small but statistically significant effect. Here, capitalizing on this trivial cleaning step will allow experiments to relax their requirements on the allowable air exposure time during construction, leading to cost and time savings.

Radioactivity↗

Utilization of traceable standards to validate plutonium isotopic purification and separation of plutonium progeny using AG MP-1M resin for nuclear forensic investigations

Radio-chronometric studies on plutonium (Pu) materials require independent measurement of the Pu (parent) content and isotopic distribution as well as concentration and isotopic distribution of the plutonium isotopic decay products. We performed a series of experiments to demonstrate the consistency of separations using the Lewatit MP 800 macroporous anion exchange resin and the AG MP-1M resin with traceable Pu isotopic certified reference material (CRM) standards 136, 137, 138, and 126-A. Two different mesh-sizes of the AG MP-1M resin were tested and the 50–100 mesh size resin was found to work more efficiently for the separation task. Both Lewatit and AG MP-1M resins were found to perform satisfactorily for quantitatively extracting the americium (Am) and uranium (U) progeny as well as gallium (Ga) present as a tracer in the Pu material. Both resins were effective in removing isobaric interferences from the Pu fraction used in isotopic measurements by thermal ionization mass spectrometry (TIMS). To address the co-elution of uranium and gallium, Alizarin red S (ARS) was used as a colorimetric dye to determine the behavior of UO 2 2+ and Ga 3+ on AG MP-1M resin with various acidic solutions as eluents using UV–vis spectra. Poor resolution of these peaks complicated quantitative analysis by UV–vis spectroscopy, but these results were informative in planning automated separation experiments by HPLC. LA-UR-24-28919.

38 RADIATION CHEMISTRY, RADIOCHEMISTRY, AND NUCLEA↗

Fractionation of UF6 and daughter progeny in storage cylinders from external heating

Abstract Uranium hexafluoride (UF 6 ) is a significant concern for material accountancy and verification in the international safeguards community. Verification of the contents of UF 6 cylinders is generally attempted with gamma spectroscopy but the current methods assume a uniform, homogeneous UF 6 mass distribution within the cylinder. In this work, it was found experimentally and confirmed via modeling, that under an external heat load (the sun), the UF 6 and its daughter products undergo fractionation in the cylinder. This fractionation of the UF 6 and daughter products can cause an errant measurement of the enrichment of the cylinder when using the current verification methods.

38 RADIATION CHEMISTRY, RADIOCHEMISTRY, AND NUCLEA↗

Accelerating Surface Radiation Content to Investigate the Impact of Radon Progeny on Superconducting Qubits

Ionizing radiation in the form of $α$, $β$, $γ$, and additional high-energy particles can induce decoherence via phonon and quasiparticle poisoning in superconducting qubits. Recent studies have explored this effect using cosmic rays or controlled radioactive sources held in the proximity of a qubit package, and have concluded that reductions in such ``external'' environmental radiation may benefit stable operation of qubit devices. However, the effect of long-lived, unstable daughters of $^{222}$Rn that ``plate out'' directly on device and packaging surfaces has not been as extensively explored. This plate-out process, well-known to the dark matter direct detection field, occurs throughout the fabrication and testing lifecycle of a device and (separately) its packaging, and produces a local source of $α$-decays which can remain active for decades. As this scales with chip area, understanding and managing this source of ionizing radiation is relevant for successfully scaling quantum computing architectures to larger numbers of qubits in a radiation-robust way. We present a setup capable of accelerating and enhancing radon daughter plateout by a factor of $7\times10^4$ over ambient, in order to study, \textit{in situ}, the impact of these events on superconducting qubits. We also provide outlook on the potential impact of this source of ionizing radiation on current and future qubit arrays.

Poudel, Sagar S. [South Dakota Sch. Mines Tech.]↗

Challenges and Practical Solutions for Conducting Structural MARSSIM Surveys in Elevated Radon Environments - 20413

The Multi-Agency Radiation Survey and Site Investigation Manual (MARSSIM) [1] provides comprehensive guidance for the development and implementation of radiological surveys conducted to demonstrate compliance with a dose- or risk-based cleanup goal during final status survey. The MARSSIM methodology follows an integrated strategy combining direct measurements and sampling to assess uniform residual contamination, and scanning surveys to identify localized areas of elevated activity. Building surveys assessing residual surface contamination present unique challenges from reliance on field measurements that may be subject to less than ideal environmental conditions. Additionally, limited characterization and excessive conservatism incorporated into the conceptual site model may result in derived concentration guideline levels (DCGL) and, as a consequence of relatively inefficient field instrumentation, corresponding attributable count rates generally comparable to background. The presence of elevated ambient radon concentrations and associated progeny deposition both exacerbate these intrinsic survey challenges and introduce a series of technical, logistic, and administrative obstacles that can wreak havoc on the statistical legitimacy of each component of the MARSSIM process. Short-lived radon progeny including polonium-218, lead-214, bismuth-214, and polonium-214 produce excessive alpha and beta radiation that may decrease instrument sensitivity through an increase in background count rates, and, at worst, may result in incorrect survey unit failure through the simulation of residual surface activity. Aside from the obvious solution of exhaustive radon mitigation, the primary objectives are to distinguish between radon progeny deposition and actual surface activity, and collect sufficient defensible data. The following is a technical discussion of the consequences of performing building MARSSIM surveys in the presence of elevated ambient radon atmospheres, conceptual examples, and an overview of practical strategies to minimize the effects of radon interference while collecting sufficient defensible data to complete the survey. (authors)

12 MANAGEMENT OF RADIOACTIVE AND NON-RADIOACTIVE W↗

Genomic prediction of switchgrass winter survivorship across diverse lowland populations

Abstract In the North-Central United States, lowland ecotype switchgrass can increase yield by up to 50% compared with locally adapted but early flowering cultivars. However, lowland ecotypes are not winter tolerant. The mechanism for winter damage is unknown but previously has been associated with late flowering time. This study investigated heading date (measured for two years) and winter survivorship (measured for three years) in a multi-generation population generated from two winter-hardy lowland individuals and diverse southern lowland populations. Sequencing data (311,776 markers) from 1,306 individuals were used to evaluate genome-wide trait prediction through cross-validation and progeny prediction (n = 52). Genetic variance for heading date and winter survivorship was additive with high narrow-sense heritability (0.64 and 0.71, respectively) and reliability (0.68 and 0.76, respectively). The initial negative correlation between winter survivorship and heading date degraded across generations (F1 r = −0.43, pseudo-F2 r = −0.28, pseudo-F2 progeny r = −0.15). Within-family predictive ability was moderately high for heading date and winter survivorship (0.53 and 0.52, respectively). A multi-trait model did not improve predictive ability for either trait. Progeny predictive ability was 0.71 for winter survivorship and 0.53 for heading date. These results suggest that lowland ecotype populations can obtain sufficient survival rates in the northern United States with two or three cycles of effective selection. Despite accurate genomic prediction, naturally occurring winter mortality successfully isolated winter tolerant genotypes and appears to be an efficient method to develop high-yielding, cold-tolerant switchgrass cultivars.

60 APPLIED LIFE SCIENCES↗

Efficacy of a Short-Term Captive Broodstock Program Compared with Hatchery-Origin Spring Chinook Salmon Derived from the Same Population

Abstract We examined the efficacy of a one-generation (five brood years: 1997–2001) captive broodstock program for spring Chinook Salmon Oncorhynchus tshawytscha by comparing survival rates of captive broodstock progeny (CBP; F2) with that of hatchery-origin fish (HOR) from a conservation hatchery supplementation program in which both groups were derived from the Tucannon River (Washington State) population for the 2000–2006 brood years. Survival rates compared were egg to fry, fry to smolt, egg to smolt, total (ages 3–5) and adult (ages 4+) smolt-to-adult-return (SAR) survival, and total (ages 3–5) and adult (ages 4+) progeny-to-parent (P:P) ratio. Total escapement and adult P:P ratios were also examined to determine if observed demographic benefits to the population continued after the captive broodstock program ended. The CBP group had lower within-hatchery survival than the HOR group, with significant differences in survival at the egg-to-fry and egg-to-smolt stages due to poor egg viability. Mean untransformed total and adult SARs for the CBP were half those of the HOR group; however, SARs did not differ significantly. The CBP also had significantly lower total and adult P:P ratios than the HOR group and were below replacement for six of the seven brood years. While the captive broodstock provided additional fish for release that would not have been available otherwise, overall the CBP performed poorly and below expectations compared with the HOR group, both within the hatchery and after release. The captive broodstock program provided a short-term demographic boost, most notable in the 2008–2010 return years, but the benefit did not carry over after the program ended.

59 BASIC BIOLOGICAL SCIENCES↗

Skinny kelp (Saccharina angustissima) provides valuable genetics for the biomass improvement of farmed sugar kelp (Saccharina latissima)

Abstract Saccharina latissima (sugar kelp) is one of the most widely cultivated brown marine macroalgae species in the North Atlantic and the eastern North Pacific Oceans. To meet the expanding demands of the sugar kelp mariculture industry, selecting and breeding sugar kelp that is best suited to offshore farm environments is becoming necessary. To that end, a multi-year, multi-institutional breeding program was established by the U.S. Department of Energy's (DOE) Advanced Research Projects Agency-Energy (ARPA-E) Macroalgae Research Inspiring Novel Energy Resources (MARINER) program. Hybrid sporophytes were generated using 203 unique gametophyte cultures derived from wild-collected Saccharina spp . for two seasons of farm trials (2019–2020 and 2020–2021). The wild sporophytes were collected from 10 different locations within the Gulf of Maine (USA) region, including both sugar kelp ( Saccharina latissima ) and the skinny kelp species ( Saccharina angustissima ). We harvested 232 common farm plots during these two seasons with available data. We found that farmed kelp plots with skinny kelp as parents had an average increased yield over the mean (wet weight 2.48 ± 0.90 kg m −1 and dry weight 0.32 ± 0.10 kg m −1 ) in both growing seasons. We also found that blade length positively correlated with biomass in skinny kelp x sugar kelp crosses or pure sugar kelp crosses. The skinny x sugar progenies had significantly longer and narrower blades than the pure sugar kelp progenies in both seasons. Overall, these findings suggest that sugar x skinny kelp crosses provide improved yield compared to pure sugar kelp crosses.

59 BASIC BIOLOGICAL SCIENCES↗

Excessive radon-based radiation in indoor air caused by soil building materials in traditional homes on Đồng Văn karst plateau, northern Vietnam

Radon-based radiation from natural soil building materials is an important factor likely influencing residents’ health as a contributing source of natural radiation. This work aims to quantify the nuclide-specific α-radiation of isotopes 222 Rn and 220 Rn in common types of houses in a region of northern Vietnam, Ɖông Văn karst plateau, to preliminarily (i) evaluate the total annual effective dose rates and (ii) assess the relative risk of cancer induction from indoor α-radiation for inhabitants. The average 222 Rn concentrations in all house types were lower than 100 Bq m -3 , but 220 Rn abundances were far higher than 222 Rn, even up to >1000 Bq m -3 in air close to a wall of unfired-soil bricks. The estimated total annual effective dose rates from indoor 222 Rn and 220 Rn and their progenies to residents with daily exposure of 13 h in the various types of houses range from 3.1 to 4.3 mSv a -1 for houses constructed with modified materials, but up to higher than 6 mSv a -1 in houses with raw building materials. The average risk of developing lung cancer as a consequence of a lifetime exposure to indoor α-radiation in affected homes ranges from 3.9% to 14.6%. 220 Rn and its metallic progenies contribute more than 80% of the total average lung cancer risk from total radon, being responsible for a range of 2.7–14.6% of the risk of developing lung cancer.

54 ENVIRONMENTAL SCIENCES↗

Basis for the ICRP’s updated biokinetic model for systemic astatine

The International Commission on Radiological Protection (ICRP) recently updated its biokinetic models for workers in a series of reports called the OIR (occupational intakes of radionuclides) series. A new biokinetic model for astatine (At), the heaviest member of the halogen family, was adopted in OIR Part 5 (ICRP in press). Occupational intakes of radionuclides: Part 5). Furthermore, this paper provides an overview of available biokinetic data for At; describes the basis for the ICRP's updated model for At; and tabulates dose coefficients for intravenous injection of each of the two longest lived and most important At isotopes, 211 At and 210 At. At-211 (T 1/2 = 7.214 h) is a promising radionuclide for use in targeted α-particle therapy due to several favourable properties including its half-life and the absence of progeny that could deliver significant radiation doses outside the region of α-particle therapy. At-210 (T 1/2 = 8.1 h) is an impurity generated in the production of 211 At in a cyclotron and represents a potential radiation hazard via its long-lived progeny 210 Po (T 1/2 = 138 days). Tissue dose coefficients for injected 210 At and 211 At based on the updated model are shown to differ considerably from values based on the ICRP's previous model for At, particularly for the thyroid, stomach wall, salivary glands, lungs, spleen, and kidneys.

61 RADIATION PROTECTION AND DOSIMETRY↗