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Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

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At least 19 records

Optimizing Selection Pressures and Pest Management to Maximize Cultivation Yield (OSPREY) (Final Technical Report)

This project was proposed in response to AOI 1, Cultivation Intensification Processes for Algae, within the FY19 Bioenergy Technologies Office Multi-Topic Funding Opportunity Announcement (FOA Number: DE-FOA-0002029). The work was designed to address a critical industry need to improve annualized productivity, stability, and quality of algal production strains for biofuels and bioproducts. The overall project goals were to generate process innovations rooted in established outdoor systems for strain selection, improvement, maintenance, and cultivation as well as pest detection and tracking. Planned advances included a 50% improvement in harvest yield based on AFDW (g m 2 d -1 ), 50% improvement in robustness based on stability metrics (e.g., high-productivity cultivation days, pond uptime), and 20% improvement in conversion yield. Individually, each of our planned process improvements (e.g., pest tracking) had the potential to increase productivity. However, to realize increases in yield at the system level, improvements to one unit’s process must be balanced against potential effects on other processes. For example, changes to strains, cultivation, and pest management developed in isolation may hurt other unit operations. Therefore, a critical success factor of the project was the integration of the pipeline components, achieved through iterative field-to- (short term) lab testing. In addition, through sustainability models, we evaluated how improvements would alter industry scenarios.

09 BIOMASS FUELS↗

Integrated Pest Management (IPM) for Early Detection Algal Crop Protection. Final Report

During large-scale, outdoor algal biomass growth, production strain(s) are subject to attack by pathogens, predators, and non-productive competitors, compromising the biomass yield. The project team at UCSD developed and demonstrated the effectiveness of Volatile Organic Compound, VOC, analysis via mass spectrometry (MS) as an early detection system for the presence of production algal ponds infection. The MS detection can then trigger high-resolution melt analysis (HRMA) and enhanced quantitative PCR (qPCR) to identify predatory or pathogenic species precisely. This study gathered the foundational data informing how wholly automated systems monitor the health of production ponds. By detecting the presence of low molecular weight VOCs in the air space above ponds, the instrument was able to mimic “sniffing” the health of the algae pond. This early warning system will be free growers from time-consuming and costly regular surveillance of ponds with the current techniques, such as qPCR and flow-cam assays, which are slower, less sensitive, and not easily fully automated, resulting in increased production efficiency through the reduction of crop loss. This project demonstrated that Chemical Ionization Mass Spectrometry, CIMS, could serve as a real-time monitor of ponds health tracking up to 100 different VOCs simultaneously for continuous periods up to 60 days without interruption, signaling infection within an algal crop 24 to 36 hours prior to techniques such as qPCR and microscopy. While mass spectrometry is an expensive tool, this simple VOC sampling system proved not only to be sensitive and robust but could to multiplexed to monitor several ponds, lowering the overall cost serially. This technology demonstrated a reduction in biomass production cost by approximately 50% compared to growers not employing any detection methodology.

09 BIOMASS FUELS↗

AmeriFlux US-MN3 Morris: Corn-Soybean, Conventional Tillage

This is the AmeriFlux version of the carbon flux data for the site US-MN3 Morris: Corn-Soybean, Conventional Tillage. Site Description - Cropland, 2-year rotation (corn-soybean), conventional (deep rip) tillage, uniform nutrient management, uniform pest management, tile drainage

Helseth, Christina↗

AmeriFlux FLUXNET-1F US-MN3 Morris: Corn-Soybean, Conventional Tillage

This is the AmeriFlux Management Project (AMP) created FLUXNET-1F version of the carbon flux data for the site US-MN3 Morris: Corn-Soybean, Conventional Tillage. This is the FLUXNET version of the carbon flux data for the site US-MN3 Morris: Corn-Soybean, Conventional Tillage produced by applying the standard ONEFlux (1F) software. Site Description - Cropland, 2-year rotation (corn-soybean), conventional (deep rip) tillage, uniform nutrient management, uniform pest management, tile drainage

Helseth, Christina [USDA-ARS LTAR]↗

AmeriFlux US-MN1 Morris: Corn-Soybean with Cover Crops, Strip Tillage

This is the AmeriFlux version of the carbon flux data for the site US-MN1 Morris: Corn-Soybean with Cover Crops, Strip Tillage. Site Description - Cropland, 2-year rotation (corn-soybean), strip tillage with winter cover crops (annual rye), nutrient management by soil tests, uniform pest management, tile drainage

Helseth, Christina↗

AmeriFlux US-MN2 Morris: Corn-Soybean-Wheat with Cover Crops, Minimal Tillage

This is the AmeriFlux version of the carbon flux data for the site US-MN2 Morris: Corn-Soybean-Wheat with Cover Crops, Minimal Tillage. Site Description - Cropland, 3-year rotation (corn-soybean-wheat), cover crops (winter camelina and pennycress), minimum tillage, nutrient management by soil tests, uniform pest management, tile drainage

Helseth, Christina↗

AmeriFlux FLUXNET-1F US-MN1 Morris: Corn-Soybean with Cover Crops, Strip Tillage

This is the AmeriFlux Management Project (AMP) created FLUXNET-1F version of the carbon flux data for the site US-MN1 Morris: Corn-Soybean with Cover Crops, Strip Tillage. This is the FLUXNET version of the carbon flux data for the site US-MN1 Morris: Corn-Soybean with Cover Crops, Strip Tillage produced by applying the standard ONEFlux (1F) software. Site Description - Cropland, 2-year rotation (corn-soybean), strip tillage with winter cover crops (annual rye), nutrient management by soil tests, uniform pest management, tile drainage

Helseth, Christina [USDA-ARS LTAR]↗

AmeriFlux FLUXNET-1F US-MN2 Morris: Corn-Soybean-Wheat with Cover Crops, Minimal Tillage

This is the AmeriFlux Management Project (AMP) created FLUXNET-1F version of the carbon flux data for the site US-MN2 Morris: Corn-Soybean-Wheat with Cover Crops, Minimal Tillage. This is the FLUXNET version of the carbon flux data for the site US-MN2 Morris: Corn-Soybean-Wheat with Cover Crops, Minimal Tillage produced by applying the standard ONEFlux (1F) software. Site Description - Cropland, 3-year rotation (corn-soybean-wheat), cover crops (winter camelina and pennycress), minimum tillage, nutrient management by soil tests, uniform pest management, tile drainage

Helseth, Christina [USDA-ARS LTAR]↗

A Biomass Pyrolysis Oil as a Novel Insect Growth Regulator Mimic for a Variety of Stored Product Beetles

Abstract As fumigants face increasing regulatory restrictions, resistance, and consumer pushback, it is vital to expand the integrated pest management (IPM) chemical toolkit for stored products. The production of biomass derived insecticides (e.g., bio-oil fraction) from byproducts of biofuel production may be a promising alternative source of chemistries for controlling stored product insects. These potential insecticidal bio-oils were fractionated based on boiling points (ranging from 115 to 230°C in one series and 245–250°C in another). Fractions were analyzed using GC-MS, and were found to be unique in composition. The lethality of these fractions was tested on Tribolium castaneum, Tribolium confusum, and Oryzaephilus surinamensis (L.) (Coleoptera: Silvanidae). Fractions were tested at concentrations ranging from 5–260 mg/ml to screen for efficacy against adults for durations of 2–8 hr sprayed on concrete arenas. In addition, a separate assay evaluated adult emergence of larvae after 6 wk with supplemental food in arenas, while repellency was evaluated against four stored product insect species in a laminar wind tunnel. A greenhouse gas (GHG) emissions life cycle assessment was also performed, which found the use of the bio-oil fraction could reduce GHG emissions associated with the insecticide supply chain by 25–61% relative to a fossil-fuel based insecticide or pyrethroid. While adults were largely unaffected, we found that larval emergence was significantly suppressed compared to controls by roughly half or more. We also determined that there was minimal repellency to most fractions by most species. We conclude that the use of bio-oil fractions is a climate-friendly choice that may support IPM programs.

09 BIOMASS FUELS↗

Invasive wild pig ( Sus scrofa ) diets on barrier islands in the southeastern United States

BACKGROUND: Biological invasions are a leading cause of reductions in global biodiversity. Islands are particularly sensitive to invasions, which often result in cascading impacts throughout island communities. Wild pigs (Sus scrofa) are globally invasive and pose threats to numerous taxa and ecosystems, particularly for islands where they have contributed to declines of many endemic species. However, the impacts of wild pig diet on the flora and fauna remain understudied in many island systems. RESULTS: We used DNA metabarcoding of wild pig fecal samples to quantify the seasonal diet composition of wild pigs on three barrier islands in the southeastern United States. Wild pigs exhibited a diverse diet dominated by plants, but also including marine and terrestrial animals. The diet composition of plants varied seasonally and between islands. Consumption of invertebrates also changed seasonally, with a shift to coastal invertebrates, particularly crabs, in spring and summer. Vertebrates were found in <10% of samples, but spanned broad taxa including amphibians, fish, mammals, and reptiles. Species consumed by wild pigs indicate that wild pigs use a variety of habitats within barrier islands for foraging, including maritime forests, saltmarshes, and beaches. CONCLUSIONS: An observed shift to beach foraging during sea turtle nesting season suggests wild pigs have potential to hinder nesting success on islands without established management programs. These findings provide insight into the diverse diets of wild pigs on barrier islands and highlight the need for removal of wild pigs from sensitive island ecosystems because of their potential impacts to native plant and animal communities. © 2024 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

60 APPLIED LIFE SCIENCES↗

AmeriFlux FLUXNET-1F US-CF1 CAF-LTAR Cook East

This is the AmeriFlux Management Project (AMP) created FLUXNET-1F version of the carbon flux data for the site US-CF1 CAF-LTAR Cook East. This is the FLUXNET version of the carbon flux data for the site US-CF1 CAF-LTAR Cook East produced by applying the standard ONEFlux (1F) software. Site Description - CF1 has operated since May 2017 at the R.J. Cook Agronomy Farm outside of Pullman, Washington, and monitors the no-till side of a paired-catchment study that is part of the Longterm Agroecosystem Research (LTAR) site common experiment. CF1 is located in “Cook East,” a field that has been in no-till management since 1998, and represents the Alternative treatment of the LTAR common experiment. It is paired with the CF2 tower location in “Cook West,” which has been in conventional tillage since prior to 1998 and represents the Prevalent treatments of the common experiment. Cook Agronomy Farm is in the high precipitation agroecological zone of the Columbia Plateau’s dryland cropping region. Crop rotations are wheat-based and include winter wheat, spring wheat, chickpea, spring canola, and winter peas. Wheat is the principal cash crop, with other crops grown in rotation for diversity, nutrient, and pest management. Soils are predominantly silt loam texture Mollisols in the Palouse, Thatuna, Naff soil series. The CF1 tower replaced US-RC1, which operated 2012-2016 in a neighboring field that was also in no-till management since 1998. CF1 and RC1 have distinct footprints, aspects, and soil series compositions.

Phillips, Claire L.↗

Reliability metrics and their management implications for open pond algae cultivation

The prevalence of contaminating organisms in outdoor algae cultivation, with the often-associated dramatic crop failures, necessitates the need for metrics describing production system reliability. Standard metrics for algae cultivation reliability are critically needed to be able to map improvements in operational parameters, but do not currently exist. In this work, we present a set of standard metrics including mean time to failure (MTTF) and mean time between failures (MTBF) as the basis for the calculation of pond failure rate (FR) and reliability coefficient (RC). Metrics associated with the numbers of contaminating organisms such as abundance ratio (AR), prevalence of infection (PI), and mean intensity of infection (MII) are also relevant. These metrics, based on measured experimental values of outdoor pond performance during the Algae Testbed Public-Private Partnership (ATP3) Unified Field Studies (UFS), provide a basis for quantifying pond failure and provide insight into potential pond management and contaminant mitigation strategies. From these reliability metrics applied to this dataset, we are able to link operational parameters, such as harvest frequency and inoculum source, to pond reliability for different algae strains and seasons, and from an assessment of AR, provide contamination thresholds beyond which a culture may be unrecoverable. Ultimately, the implementation of widely-practiced and simple-to-calculate algae pond reliability metrics calculated from rapid and easy to collect data or observations will reduce risk and uncertainty in large-scale algae deployment and aid in the development of integrated pest management (IPM) strategies.

09 BIOMASS FUELS↗

A New Repellent for Redbay Ambrosia Beetle (Coleoptera: Curculionidae: Scolytinae), Primary Vector of the Mycopathogen That Causes Laurel Wilt

The redbay ambrosia beetle, Xyleborus glabratus, was detected in Georgia, USA, in 2002 and has since spread to 11 additional states. This wood-boring weevil carries a symbiotic fungus, Harringtonia lauricola, that causes laurel wilt, a lethal disease of trees in the Lauraceae family. Native ambrosia beetles that breed in infected trees can acquire H. lauricola and contribute to the spread of laurel wilt. Since 2002, laurel wilt has devastated native Persea species in coastal forests and has killed an estimated 200,000 avocado trees in Florida. Since laurel wilt is difficult to manage once it has entered a susceptible agrosystem, this study evaluated piperitone as a candidate repellent to deter attacks by X. glabratus and other ambrosia beetles. Additionally, piperitone was compared to the known repellent verbenone as a potential cost-effective alternative. The repellent efficacy was determined by comparing captures in traps baited with commercial beetle lures containing α-copaene versus captures in traps baited with lures plus a repellent. In parallel 10-week field tests, the addition of piperitone reduced the captures of X. glabratus in α-copaene-baited traps by 90%; however, there was no significant reduction in the captures of native ambrosia beetles in ethanol-baited traps. In two replicate 10-week comparative tests, piperitone and verbenone both reduced X. glabratus captures by 68–90%, with longevity over the full 10 weeks. This study identifies piperitone as a new X. glabratus repellent with potential for pest management.

59 BASIC BIOLOGICAL SCIENCES↗

Predicting plant attractiveness to coccinellids with plant trait profiling, citizen science, and common garden surveys

Lady beetles are key predators in agricultural systems and their impact can be enhanced by plant resources. Facilitation of lady beetles is a potentially effective pest management strategy but a lack of data on which plant species attract and nurture them limits implementation. Here we investigate which plant species might optimize lady beetle impact by developing a profile of plant characteristics most likely to attract lady beetles and testing if the subset of “potentially preferred” plant families exhibiting those characteristics are significantly more attractive to lady beetles than other families. This testing encompassed a combination of observations submitted to a citizen science program, the Lost Ladybug Project, and two repeated site surveys. Traits reported to be most attractive to lady beetles included the presence of trichomes that provide protection from predation, nutritional resources such as nectar or prey, and visual or chemical cues associated with those traits. Significantly more lady beetles were observed on the three plant families which most often exhibit these traits, Apiaceae, Asteraceae, and Rosaceae, than on other families. Furthermore, strategies for incorporating plants from these families into production systems to attract lady beetles and limit pest damage are discussed.

59 BASIC BIOLOGICAL SCIENCES↗

Linking Small-Scale Flight Manoeuvers and Density Profiles to the Vertical Movement of Insects in the Nocturnal Stable Boundary Layer

Huge numbers of insects migrate over considerable distances in the stably-stratified night-time atmosphere with great consequences for ecological processes, biodiversity, ecosystem services and pest management. We used a combination of meteorological radar and lidar instrumentation at a site in Oklahoma, USA, to take a new look at the general assistance migrants receive from both vertical and horizontal airstreams during their long-distance flights. Movement in the nocturnal boundary layer (NBL) presents very different challenges for migrants compared to those prevailing in the daytime convective boundary layer, but we found that Lagrangian stochastic modelling is effective at predicting flight manoeuvers in both cases. A key feature for insect transport in the NBL is the frequent formation of a thin layer of fast-moving air – the low-level jet. Modelling suggests that insects can react rapidly to counteract vertical air movements and this mechanism explains how migrants are retained in the jet for long periods (e.g. overnight, and perhaps for several hours early in the morning). This results in movements over much longer distances than are likely in convective conditions, and is particularly significant for the reintroduction of pests to northern regions where they are seasonally absent due to low winter temperatures.

54 ENVIRONMENTAL SCIENCES↗