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Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

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At least 19 records
All sourcesNASA NTRSDOE OSTI
NASA NTRS2019

Plant Microbiomes May Provide Vital Information to Plant Success

Plant associated microbiomes, the rhizosphere and phyllosphere, are composed of communities of bacteria and fungi that may be mutualistic or pathogenic. These communities have the potential to influence plant health and development and can affect plant growth. Crop plants are being investigated as a fresh and safe supplement to astronauts’ diet and it is critical to understand and characterize these microbial communities. Multi-species crops, Mizuna mustard (Brassica rapa var japonica), ‘Outredgeous’ red romaine lettuce (Lactuca sativa), and Waldman’s Green lettuce (Lactuca sativa) were grown in two Veggie units on the International Space Station (ISS) for three grow outs in various combinations of plant types. Upon harvest, plant and pillow samples were frozen and returned to Earth for analysis. Bacterial and fungal community analyses for plant leaf and root, as well as pillow components, wick and media, were completed using next generation sequencing with the goal of surveying the composition of the entire community and identifying any potential pathogens. Bacteria were identified using the 16S rRNA gene whereas, fungi were identified with the internal transcribed spacer (ITS). The community composition for these three crops was compared between crop types and between plant tissue types. It is vital to mission success for the short term and long term to add nutritious, safe to eat vegetables providing a supplement to the crew members’ dietary requirements as well as to develop planning for deep space missions as we reach for the moon and on to Mars. Veggie technology validation tests were supported by NASA’s Space Biology Program.

Khodadad, Christina L.↗
NASA NTRS1976

Digital registration of topographic data and satellite MSS data for augmented spectral analysis

Results are presented for a project directed to assess the type and extent of long-term natural and man-made changes in floodplain features of a definite area in the Mississipi River Valley. 1:6000 scale maps were prepared from remotely sensed film/filter photographic coverage of the area at different times to compare changes in such features as channels, backwaters, vegetation as well as changes in the amount, location, and conditions of dredge spoil. Changes observed by this sequential photo comparison include abandonment of agricultural land followed by orderly secondary plant succession, the filling-in of shallows by sediment and the orderly succession of plants from a hydric to a more mesic environment and changes in deltas of small tributary streams in response to agricultural practices occurring at their headwaters. Plant colonization is observed on some spoil, and secondary movement of spoil is revealed in other areas.

Anuta, P. E.↗
NASA NTRS1974

Vegetation mapping from ERTS imagery of the Okavango Delta

The Okavango is Botswana's major water resource. The present study has been specifically directed at mapping vegetation types within the delta and generally concerned with finding what information of value to plant and animal ecologists could be extracted from the imagery. To date it has been found that. (1) It is possible to map broad vegetation types from the imagery. (2) Imagery of the delta records the state of the system in a manner which will facilitate long-term studies of plant succession. (3) Phenological events can be detected. (4) The imagery can be used to detect and map wild fires. This will be useful in determining the role of fire in the ecology of the region. Using the imagery it is thus possible to map existing vegetation and monitor both short and long-term changes.

Willamson, D. T.↗
NASA NTRS2000

Influence of microgravity on ultrastructure and storage reserves in seeds of Brassica rapa L

Successful plant reproduction under spaceflight conditions has been problematic in the past. During a 122 d opportunity on the Mir space station, full life cycles of Brassica rapa L. were completed in microgravity in a series of three experiments in the Svet greenhouse. Ultrastructural and cytochemical analyses of storage reserves in mature dry seeds produced in these experiments were compared with those of seeds produced during a high-fidelity ground control. Additional analyses were performed on developing Brassica embryos, 15 d post pollination, which were produced during a separate experiment on the Shuttle (STS-87). Seeds produced on Mir had less than 20% of the cotyledon cell number found in seeds harvested from the ground control. Cytochemical localization of storage reserves in mature cotyledons showed that starch was retained in the spaceflight material, whereas protein and lipid were the primary storage reserves in ground control seeds. Protein bodies in mature cotyledons produced in space were 44% smaller than those in the ground control seeds. Fifteen days after pollination, cotyledon cells from mature embryos formed in space had large numbers of starch grains, and protein bodies were absent, while in developing ground control seeds at the same stage, protein bodies had already formed and fewer starch grains were evident. These data suggest that both the late stage of seed development and maturation are changed in Brassica by growth in a microgravity environment. While gravity is not absolutely required for any step in the plant life cycle, seed quality in Brassica is compromised by development in microgravity.

NASA Experiment Number 9600004↗
NASA NTRS2004

Innovative Technology Reduces Power Plant Emissions - Commercialization Success

Emission control system development includes: (1) Development of new oxidizer scrubber system to eliminate NOx waste and produce fertilizer (2) Technology licensed and a 1 to 3 MWatt-scale prototype installed on. power plant (3) Development of method to oxidize NO. to N02 (4) Experience gained from licensing NASA technology

Parrish, Clyde↗
NASA NTRS2004

Innovative Technology Reduces Power Plant Emissions-Commercialization Success

Overview of emission control system development: (1) Development of new oxidizer scrubber system to eliminate NOx waste and produce fertilizer (2) Technology licensed and a 1 to 3 MWatt-scale prototype installed on power plant (3) Development of method to oxidize NO to NO2 (4) Experience gained from licensing NASA technology.

Parrish, Clyde↗
NASA NTRS1983

Birth of space plant growing

The attempts, and successes, to grow plants in space, and get them to fully develop, bloom and produce seeds using orchids are presented. The psychological advantages of the presence of plants onboard space vehicles and space stations is indicated.

Mashinskiy, A.↗
NASA NTRS2013

VEG-01: Veggie Hardware Verification Testing

The Veggie plant/vegetable production system is scheduled to fly on ISS at the end of2013. Since much of the technology associated with Veggie has not been previously tested in microgravity, a hardware validation flight was initiated. This test will allow data to be collected about Veggie hardware functionality on ISS, allow crew interactions to be vetted for future improvements, validate the ability of the hardware to grow and sustain plants, and collect data that will be helpful to future Veggie investigators as they develop their payloads. Additionally, food safety data on the lettuce plants grown will be collected to help support the development of a pathway for the crew to safely consume produce grown on orbit. Significant background research has been performed on the Veggie plant growth system, with early tests focusing on the development of the rooting pillow concept, and the selection of fertilizer, rooting medium and plant species. More recent testing has been conducted to integrate the pillow concept into the Veggie hardware and to ensure that adequate water is provided throughout the growth cycle. Seed sanitation protocols have been established for flight, and hardware sanitation between experiments has been studied. Methods for shipping and storage of rooting pillows and the development of crew procedures and crew training videos for plant activities on-orbit have been established. Science verification testing was conducted and lettuce plants were successfully grown in prototype Veggie hardware, microbial samples were taken, plant were harvested, frozen, stored and later analyzed for microbial growth, nutrients, and A TP levels. An additional verification test, prior to the final payload verification testing, is desired to demonstrate similar growth in the flight hardware and also to test a second set of pillows containing zinnia seeds. Issues with root mat water supply are being resolved, with final testing and flight scheduled for later in 2013.

Massa, Gioia↗
NASA NTRS2021

Persistence of a Potential Pathogen on a Plant Microbiome-Does Seed Sanitization Matter?

Microbial interactions on, in, and around the seeds can have profound effects on plant growth, development, and productivity. These interactions can be casual or intimate in nature, but ultimately they all contribute in varying degrees to an ever-evolving microbiome. Plant microbiomes have been investigated over decades and new data continue to reveal how microbiomes play an important role in the plant’s success. Many eubacteria and fungi have been found to have a symbiotic relationship with plants and other microorganisms. The source of the plant microbiome on adult plants is provided by the seed via vertical transmission to leaf, root, flowers or fruit, as well as the surrounding environment.

Anirudha R. Dixit↗
NASA NTRS1998

Protocol Development for the NASA-JSC Lunar-Mars Life Support Test Project (LMLSTP) Phase 3 Project: A Report on Baseline Studies at KSC for Continuous Salad Production

The Phase 3 Lunar-Mars Life Support Test Project (LMLSTP) was conducted in a 20-foot chamber at Johnson Space Center. The overall objective of the Phase 3 project was to conduct a 90-day regenerative life support system test involving 4 human subjects to demonstrate an integrated biological and physicochemical life support system. A secondary objective of the Phase 3 LMLSTP was to demonstrate the ability to produce salad-type vegetable by integration of a small benchtop growth chamber located within the crew habitat area. This small chamber, commercially manufactured as the Controlled Environment Research Ecosystem (CERES 2010(TM)), functioned as a means to continuously provide fresh lettuce crops for crew members. The CERES 2010(TM) growth chamber utilized hardware components developed for effective plant biomass production in spaceflight applications. These components included: (1) LED lighting; (2) Astroculture(TM) Root Trays; and (3) Zeoponic media. In planning for the LMLSTP Phase 3, a request was put forward for KSC scientists to generate a protocol for successful continuous planting, culturing, and harvesting of the salad-crop, lettuce. By conducting baseline tests with components of the CERES 2010(TM), a protocol was developed.

Goins, G. D.↗
NASA NTRS2003

An in silico assessment of gene function and organization of the phenylpropanoid pathway metabolic networks in Arabidopsis thaliana and limitations thereof

The Arabidopsis genome sequencing in 2000 gave to science the first blueprint of a vascular plant. Its successful completion also prompted the US National Science Foundation to launch the Arabidopsis 2010 initiative, the goal of which is to identify the function of each gene by 2010. In this study, an exhaustive analysis of The Institute for Genomic Research (TIGR) and The Arabidopsis Information Resource (TAIR) databases, together with all currently compiled EST sequence data, was carried out in order to determine to what extent the various metabolic networks from phenylalanine ammonia lyase (PAL) to the monolignols were organized and/or could be predicted. In these databases, there are some 65 genes which have been annotated as encoding putative enzymatic steps in monolignol biosynthesis, although many of them have only very low homology to monolignol pathway genes of known function in other plant systems. Our detailed analysis revealed that presently only 13 genes (two PALs, a cinnamate-4-hydroxylase, a p-coumarate-3-hydroxylase, a ferulate-5-hydroxylase, three 4-coumarate-CoA ligases, a cinnamic acid O-methyl transferase, two cinnamoyl-CoA reductases) and two cinnamyl alcohol dehydrogenases can be classified as having a bona fide (definitive) function; the remaining 52 genes currently have undetermined physiological roles. The EST database entries for this particular set of genes also provided little new insight into how the monolignol pathway was organized in the different tissues and organs, this being perhaps a consequence of both limitations in how tissue samples were collected and in the incomplete nature of the EST collections. This analysis thus underscores the fact that even with genomic sequencing, presumed to provide the entire suite of putative genes in the monolignol-forming pathway, a very large effort needs to be conducted to establish actual catalytic roles (including enzyme versatility), as well as the physiological function(s) for each member of the (multi)gene families present and the metabolic networks that are operative. Additionally, one key to identifying physiological functions for many of these (and other) unknown genes, and their corresponding metabolic networks, awaits the development of technologies to comprehensively study molecular processes at the single cell level in particular tissues and organs, in order to establish the actual metabolic context.

NASA Program Fundamental Space Biology↗
NASA NTRS1972

Manual control models of industrial management

The industrial engineer is often required to design and implement control systems and organization for manufacturing and service facilities, to optimize quality, delivery, and yield, and minimize cost. Despite progress in computer science most such systems still employ human operators and managers as real-time control elements. Manual control theory should therefore be applicable to at least some aspects of industrial system design and operations. Formulation of adequate model structures is an essential prerequisite to progress in this area; since real-world production systems invariably include multilevel and multiloop control, and are implemented by timeshared human effort. A modular structure incorporating certain new types of functional element, has been developed. This forms the basis for analysis of an industrial process operation. In this case it appears that managerial controllers operate in a discrete predictive mode based on fast time modelling, with sampling interval related to plant dynamics. Successive aggregation causes reduced response bandwidth and hence increased sampling interval as a function of level.

Crossman, E. R. F. W.↗
NASA NTRS1992

Design, test, and evaluation of three active flutter suppression controllers

Three control law design techniques for flutter suppression are presented. Each technique uses multiple control surfaces and/or sensors. The first method uses traditional tools (such as pole/zero loci and Nyquist diagrams) for producing a controller that has minimal complexity and which is sufficiently robust to handle plant uncertainty. The second procedure uses linear combinations of several accelerometer signals and dynamic compensation to synthesize the model rate of the critical mode for feedback to the distributed control surfaces. The third technique starts with a minimum-energy linear quadratic Gaussian controller, iteratively modifies intensity matrices corresponding to input and output noise, and applies controller order reduction to achieve a low-order, robust controller. The resulting designs were implemented digitally and tested subsonically on the active flexible wing wind-tunnel model in the Langley Transonic Dynamics Tunnel. Only the traditional pole/zero loci design was sufficiently robust to errors in the nominal plant to successfully suppress flutter during the test. The traditional pole/zero loci design provided simultaneous suppression of symmetric and antisymmetric flutter with a 24-percent increase in attainable dynamic pressure. Posttest analyses are shown which illustrate the problems encountered with the other laws.

Adams, William M., Jr.↗
NASA NTRS2003

Success Continues: NASA-Developed Plant Health Measurement Technology is Becoming Big Business for Illinois Company

Originally produced in 2001, sales of Spectrum Technologies' CM 1000 chlorophyll meter have now topped $290,000 on 140 units. Up-to-date sales figures for 2003 have shown an almost 50% increase over the combined sales totals of 2001 and 2002. The CM 1000 chlorophyll meter identifies the failing health of a plant based on the chlorophyll content of the plant up to 16 days before it is physically detectable by the human eye. Poor health, 'stress' in a plant, is a result of unfavorable growing conditions; lack of nutrients, insufficient water, disease or insect damage.

Source record↗
NASA NTRS2023

NASA X-HAB Water Delivery System

As part of the eXploration Systems and Habitation (X-HAB) Academic Innovation Challenge program of National Aeronautics and Space Administration (NASA), The Ohio State University was selected to improve the performance of NASA’s existing Vegetable Production System (VEGGIE), which is a deployable plant growth unit for International Space Station (ISS). During the academic year 2015-2016, The Ohio State University student team developed a passive water delivery system using capillary water transport principle (Jenson et al. 2016). The major design improvement made was directly connecting the water reservoir to the plant-rooting pillows using a single-interface capillary cord design. Harvestable plants were successfully grown from seeds using the single interface system. In addition, Nomex®, a fabric material composed of short nylon based fibers, was identified as the material for wicks. Finally, the water reservoir was modeled as a propellant management device (PMD) to ensure consistent and long term watering of the VEGGIE system. The PMDs are made of materials that utilize surface tension and adhesive forces to improve stability and fluid delivery. The team recommended using a sponge PMD in order to mitigate bubble obstruction, decrease system weight, and ensure reliable water delivery to the capillary interface.

Peter Ling↗
NASA NTRS1984

In vitro seed to seed growth on clinostats

The effect of a long term micro-gravity environment on the life cycle of plants is unknown. Whether higher plants have evolved to a stage where removal or reduction of gravity is detrimental to plant life cycle and thus fatal to the plant species, is an unanswered question in space plants which were successfully grown through the various stages of their life cycle. Attempts to grow plants as a continuous integral process from seed to seed through one generation were successful until recently. Culture of plants through multiple generations was not accomplished in space nor in ground based studies. The effect of long term simulated weightlessness by growing consecutive generations of plants continuously on clinostats using the cruciferous plants, Arabidopsis thaliana (L.) Heyn. and Cardamine oligosperma Nutt. is being investigated.

Hoshizaki, T.↗
NASA NTRS1988

Cultural systems for growing potatoes in space

Higher plants are being evaluated for life support to provide needed food, oxygen and water as well as removal of carbon dioxide from the atmosphere. The successful utilization of plants in space will require the development of not only highly productive growing systems but also highly efficient bioregenerative systems. It will be necessary to recycle all inedible plant parts and all human wastes so that the entire complement of elemental compounds can be reused. Potatoes have been proposed as one of the desirable crops because they are 1) extremely productive, yielding more than 100 metric tons per hectare from field plantings, 2) the edible tubers are high in digestible starch (70%) and protein (10%) on a dry weight basis, 3) up to 80% of the total plant production is in tubers and thus edible, 4) the plants are easily propagated either from tubers or from tissue culture plantlets, 5) the tubers can be utilized with a minimum of processing, and 6) potatoes can be prepared in a variety of different forms for the human diet (Tibbitts et al., 1982). However potatoes have a growth pattern that complicates the development of growing the plants in controlled systems. Tubers are borne on underground stems that are botanically termed 'rhizomes', but in common usage termed 'stolons'. The stolons must be maintained in a dark, moist area with sufficient provision for enlargement of tubers. Stems rapidly terminate in flowers forcing extensive branching and spreading of plants so that individual plants will cover 0.2 m2 or more area. Thus the growing system must be developed to provide an area that is darkened for tuber and root growth and of sufficient size for plant spread. A system developed for growing potatoes, or any plants, in space will have certain requirements that must be met to make them a useful part of a life support system. The system must 1) be constructed of materials, and involve media, that can be reused for many successive cycles of plant growth, 2) involve a minimum quantity of media, 3) contain media that is essentially inert and not oxidize or degrade with use, 4) utilize a recirculating nutrient solution to permit regulation of pH and nutrient concentrations, and 5) be capable of complete automation of all planting, maintenance and harvesting procedures.

NASA Discipline Number 61-10↗