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At least 19 records

Evaluation of artificial neural network performance for classification of potato plants infected with potato virus Y using spectral data on multiple varieties and genotypes

Potato virus Y (Potyviridae, PVY) is a plant virus that poses a significant threat to potato producers on a global basis. The pathogen has disrupted seed potato supplies and negatively impacted yield and quality of commercial potato crops. The potato industry currently manages PVY infection levels via insecticide applications, regional seed certification programs that rely on field scouting to visually assess individual plants for infection status, and destructive and costly tissue sampling coupled with laboratory assays. Despite these efforts, PVY continues to confound potato industry stakeholders resulting in economic harm. Remote sensing and machine learning provide for the development of new tools to more accurately detect and spatially quantify PVY-infected plants versus the current state of the art. However, there is a need to understand how the occurrence of many different potato varieties impact the dynamics of developing models to detect potato plants impacted with PVY and their potential effectiveness. This study evaluates classification modelling outcomes using spectral datasets collected in different temporal and spatial environments (greenhouse and a production field) on multiple potato varieties consisting of labelled instances of plants infected with PVY and those not infected with the virus. A modelling framework was developed to support iterative modelling runs using artificial neural network (ANN) architectures configured as binary classifiers to develop sample populations to support statistical analysis on model performance using specific spectral subsets. When using spectral data to detect PVY-infected plants, ANN models achieved the highest mean accuracy of 0.894 on a single variety. Conversely, the same ANN model architecture only achieved a mean accuracy of 0.575 on a spectral data set representing 29 potato breeding lines. Additionally, statistical analysis indicates spectral regions including the red edge, near infrared and shortwave infrared contain more important spectral features for the ANN classifier introduced in this research.

60 APPLIED LIFE SCIENCES↗

Continuous cropping of potato changed the metabolic pathway of root exudates to drive rhizosphere microflora

For potato production, continuous cropping (CC) could lead to autotoxicity buildup and microflora imbalance in the field soil, which may result in failure of crops and reduction in yield. In this study, non-targeted metabolomics (via liquid chromatography with tandem mass spectrometry (LC–MS/MS)) combined with metagenomic profiling (via high-throughput amplicon sequencing) were used to evaluate correlations between metabolomics of potato root exudates and communities of bacteria and fungi around potato plants to illustrate the impacts of CC. Potato plants were grown in soil collected from fields with various CC years (0, 1, 4, and 7 years). Metabolomic analysis showed that the contents and types of potential autotoxins in potato root exudates increased significantly in CC4 and CC7 plants (i.e., grown in soils with 4 and 7 years of CC). The differentially expressed metabolites were mainly produced via alpha-linolenic acid metabolism in plant groups CC0 and CC1 (i.e., no CC or 1 year CC). The metabolomics of the groups CC4 and CC7 became dominated by styrene degradation, biosynthesis of siderophore group non-ribosomal peptides, phenylpropanoid biosynthesis, and biosynthesis of various plant secondary metabolites. Continuous cropping beyond 4 years significantly changed the bacterial and fungal communities in the soil around the potato crops, with significant reduction of beneficial bacteria and accumulation of harmful fungi. Correlations between DEMs and microflora biomarkers were established with strong significances. These results suggested that continuous cropping of potato crops changed their metabolism as reflected in the plant root exudates and drove rhizosphere microflora to directions less favorable to plant growth, and it needs to be well managed to assure potato yield.

Xing, Yanhong↗

Multi-level impacts of climate change and supply disruption events on a potato supply chain: An agent-based modeling approach

Context: The world is experiencing frequent extreme weather events like droughts, snowstorms, and shifting of seasons due to climate change. Increased frequency and severity of these extreme weather events threaten food security because agriculture depends on climate conditions. Impacts of climate change on the agricultural system not only occur at the grower’s level, but also cascade to other levels along the supply chain. Objective: In this study, we aim to quantify a wide range of economic impacts of different extreme climate events on different stages of a food supply chain. Methods: We chose the potato supply chain in Idaho as a case study. We developed a multi-echelon supply chain simulation model using an agent-based modeling (ABM) approach with five types of agents—farmers, shippers, processors, retailers, and logistics companies. In addition to the business-as-usual (BAU) scenario, we designed two climate-related disruption events—drought and snowstorm. We quantified the heterogeneous impacts at different stages of the supply chain for both fresh and processed potatoes using key performance indicators (KPIs) including revenues, prices, lead times, traded quantities, and food waste quantity. Results and Conclusion: The impacts of the disruption events are different on different agents in the supply chain for different product categories. The price hike of fresh potatoes is far higher than processed potatoes during disruption events. This price hike makes consumers switch to processed potatoes, which require more fresh potatoes as an input that further reinforces the price hike. However, because processed potatoes have an elastic demand, once their prices go up due to higher input cost, their demand drops. Non-contracted farmers gain additional revenues from the disruption events, whereas contracted farmers incur a loss due to lock-in price and lower than usual harvest. Significance: The methodology developed in this study could be applied to other food and agricultural supply chains for understanding the vulnerabilities at agent levels due to climate change disruption events. Furthermore, the findings would help develop mitigation strategies or policies to improve the well-being of the overall supply chain.

54 ENVIRONMENTAL SCIENCES↗

Copper ions suppress abscisic acid biosynthesis to enhance defence against Phytophthora infestans in potato

Abstract Copper‐based antimicrobial compounds are widely and historically used to control plant diseases, such as late blight caused by Phytophthora infestans , which seriously affects the yield and quality of potato. We previously identified that copper ion (Cu 2+ ) acts as an extremely sensitive elicitor to induce ethylene (ET)‐dependent immunity in Arabidopsis . Here, we found that Cu 2+ induces the defence response to P. infestans in potato. Cu 2+ suppresses the transcription of the abscisic acid (ABA) biosynthetic genes StABA1 and StNCED1 , resulting in decreased ABA content. Treatment with ABA or inhibitor fluridone made potato more susceptible or resistance to late blight, respectively. In addition, potato with knockdown of StABA1 or StNCED1 showed greater resistance to late blight, suggesting that ABA negatively regulates potato resistance to P. infestans . Cu 2+ also promotes the rapid biosynthesis of ET. Potato plants treated with 1‐aminocyclopropane‐1‐carboxylate showed enhanced resistance to late blight. Repressed expression of StEIN2 or StEIN3 resulted in enhanced transcription of StABA1 and StNCED1 , accumulation of ABA and susceptibility to P. infestans . Consistently, StEIN3 directly binds to the promoter regions of StABA1 and StNCED1 . Overall, we concluded that Cu 2+ triggers the defence response to potato late blight by activating ET biosynthesis to inhibit the biosynthesis of ABA.

Liu, Hai‐Feng↗

Limited potential of irrigation to prevent potato yield losses in Germany under climate change

Climate models project higher temperatures and a substantial net water deficit during the summer months over Germany until the end of the 21st century. The agricultural sector is particularly vulnerable to changing weather and climate conditions. In this study, we examine the role of irrigation for potato yields as a climate change adaptation measure in Northeast Lower Saxony (Germany). The region represents the largest irrigated area in Germany and is one of the main growing areas of potatoes. We develop a statistical multivariate regression model to investigate whether regional climate and irrigation data at the monthly and county level are suitable to describe potato yield variability from 1978 to 2018. Irrigation is estimated based on the climatic water balance and crop water demand. These estimates are calibrated against irrigation data from local agencies to account for realistic monthly water withdrawals. We analyze future yield changes based on climate model projections for the 21st century and different irrigation scenarios. We find that the potato yield will decrease significantly by 18% on average until 2050 with climate projection uncertainty of ±6.4% under the high emission scenario RCP 8.5 and without irrigation. Irrigation at current levels could compensate for about 54% of these yield losses. Doubling the amount of irrigation minimizes yield losses by as much as 80%. Under the low (middle) emission scenario RCP 2.6 (RCP 4.5), potato yield are projected to decrease moderately by 4.5% ± 3.4% (5.4% ± 2.8%) with irrigation at current levels. We show that the yield losses mainly originate from higher temperatures during the summer. Our analysis indicates that the capacity of irrigation to minimize yield losses is limited. Strengthening climate change mitigation efforts may be more effective in preventing yield losses. However, the effect of elevated CO 2 might buffer the high yield losses, especially in RCP 8.5. The studies supports farmers and decision makers in assessing future risks in potato yields. It can guide policymakers in allocating economic resources to cope with climate change.

60 APPLIED LIFE SCIENCES↗

Effects of different pretreatment methods on the drying characteristics and quality of potatoes

Abstract The effects of different pretreatments on the vitamin C content of peeled fresh potato, the drying characteristics, and several quality attributes of dehydrated potatoes were investigated. Citric acid pretreatment (0.1%–0.3%, 10–30 min), steam blanching (100ºC, 1–2 min), and water blanching (95°C, 1–2 min) were found to have no obvious effect on the drying rate of potatoes, whereas temperature was the main influencing factor. In terms of quality of dehydrated diced potato, 20 min of citric acid pretreatment resulted in the highest vitamin C retention and better color. Furthermore, dehydrated potato pretreated with citric acid all showed similar dynamic moisture adsorption curves, namely type II sorption isotherm. The moisture adsorption curves can be well fitted using the Guggenheim–Anderson–deBoer model with R 2 higher than .97.

Sun, Xiangfeng↗

POTATO: A Unified Assessment System for HL-LHC Outer Tracker Module Productions

The CMS detector s silicon tracker will be replaced in preparation for the upcoming High-Luminosity LHC (HL LHC) upgrade. The inner tracker will include a new pixel detector while the outer tracker (OT) will feature 5,592 pixel strip (PS) and 7,608 strip strip (2S) sensor modules with production distributed across 7 sites worldwide, requiring standardized grading and testing. One testing apparatus the burn-in box replicates the temperature cycling conditions that successful modules must withstand. The Phase-II Outer Tracker Analyzer of Test Outputs (POTATO) standardizes module evaluation and grading among testing sites. Given burn-in box test output, POTATO analyzes the results and assigns a letter grade to a module and its sub-components. POTATO's grading process must adapt to evolving standards for module evaluation; hence, we detail work on POTATO's database and grading functionality that will facilitate the unified assessment of Phase-II OT module assembly and testing worldwide. Once completed, POTATO will allow technicians to interact with a database of all graded modules and efficiently assess the global module assembly effort.

Clark, Olivia J. [U. Tennessee, Knoxville]↗

Utilization of potatoes in bioregenerative life support systems

Data on the tuberization, harvest index, and morphology of 2 cvs of white potato (Solanum tuberosum L.) grown at 12, 16, 20, 24 and 28 C, 250, 400 and 550 micromol/s/m photosynthetic photon flux (PPF), 350, 1000 and 1600 microliter 1 sup -1 CO2 is presented. A productivity of 21.9 g/m day sup -1 of edible tubers from a solid stand of potatoes grown for 15 weeks with continuous irradiation at 400 micromol/s/m, 16 C and 1000 microliter 1 sup -1 CO2 was obtained. This equates to an area of 34.3 sq m being required to provide 2800 kcal of potatoes per day for a human diet. Separated plants receiving side lighting have produced 32.8 g/m day sup -1 which equates to an area of 23.6 sq m to provide 2800 kcal. Studies with side lighting indicate that productivities in this range should be realized from potatoes. Glycoalkaloid levels in tubers of controlled environment grown plants are within the range of levels found in tubers of field grown plants. The use and limitation of recirculating solution cultures for potato growth is discussed.

Tibbitts, T. W.↗

Regulated Expression of a Calmodulin Isoform Alters Growth and Development in Potato

A transgene approach was taken to study the consequences of altered expression of a calmodutin iso-form on plant growth and development. Eight genomic clones of potato calmodulin (PCM 1 to 8) have been isolated and characterized. Among the potato calmodulin isoforms studied, PCM 1 differs from the other isoforms because of its unique amino acid substitutions. Transgenic potato plants were produced carrying sense construct of PCM 1 fused to the CAMV 35S promoter. Transgenic plants showing a moderate increase in PCM 1 MRNA exhibited strong apical dominance, produced elongated tubers, and were taller than the controls. Interestingly, the plants expressing the highest level of PCM 1 MRNA did not form underground tubers. Instead, these transgenic plants produced aerial tubers when allowed to grow for longer periods. The expression of different calmodulin isoforms (PCM 1, 5, 6, and 8) was studied in transgenic plants. Among the four potato calmodulin isoforms, only the expression of PCM 1 MRNA was altered in transgenic plants, while the expression of other isoforms was not significantly altered. Western analysis revealed increased PCM 1 protein in transgenic plants, indicating that the expression of both MRNA and protein are altered in transgenic plants. These results suggest that increasing the expression of PCM 1 alters growth and development in potato plants.

Poovaiah, B. W.↗

Development of a pilot system for converting sweet potato starch into glucose syrup

Sweet potato has been chosen as one of NASA's crops to support human beings in future space missions. One of the possible uses is to make syrup that can be used as a general sweetener. In this work a simple engineering system for converting sweet potato starch into glucose syrup was studied on a laboratory scale. The system comprises the following main units: a blender, continuous stirred tank reactor (CSTR), centrifugal and vacuum filters, deionization column and vacuum evaporator. The system was tested by carrying out conversion processes from fresh sweet potato roots. The roots were pealed, sliced, homogenized, heated and hydrolyzed by diastase of malt and Dextrozyme C (Novo Nordisk BioChem, North America, Inc.) enzymes in the CSTR. After hydrolysis the slurry was filtered, de-ionized and concentrated to get glucose syrup. The performance of the system was evaluated based on the quality of the conversion. The main factor was the level of reducing sugars except for the deionization where ash content and color were the main factors. Through careful control of the system units, good heating performance in the CSTR was obtained and the hydrolysis process attained sufficient conversion. The filtration process that incorporated the centrifuge was faster than when it was by-passed to the vacuum filter but losses in sugars were higher. Deionization removed more than 90% of the ash and reduced pigmentation, with probable insignificant losses in sugars during the deionization process. Recovery levels when the centrifuge was used and when it was by-passed could reach about 65% and 78%, respectively. These correspond to reducing sugar concentration of 259 and 310 mg/ml in 150-ml syrups from 300 g of sweet potatoes in each process. However, from concentration trials, syrups with volumes of 100 and 70 ml with the respective dextrose equivalence of 281 and 213 mg/ml were obtained. The syrups obtained were brownish in color and the process that employed centrifugal filtration gave a product with color that resembled the original color of the sweet potatoes. Further work is required to improve the overall system performance.

Non-NASA Center↗

Multi-Level Impacts of Extreme Climate-Related Events on Food Supply Chain: Idaho Potato's Case Study

Background: The world is experiencing frequent extreme weather events like droughts, floods, snowstorms, and shifting of seasons due to climate change. Increasing frequencies and severity of these extreme weather events threaten food security because agriculture is extremely vulnerable to climate change. Higher temperature reduces overall yields of the desirable crops but proliferates weeds and harmful pests. Current data shows that every year U.S. farmers spend more than 11 billion USD to fight weeds and this number is expected to rise in the coming years due to climate change. Due to droughts, the water required for irrigation is becoming scarcer. Since many areas are becoming climatically unusable for crop production, the amount of farmland area is shrinking rapidly. In addition, changes in precipitation patterns are causing crop failures and negatively impacting expected yields. In this study, we investigated how different extreme climate events impact different stages of a food supply chain. Approach: As a case study, we chose Idaho’s potato supply chain (SC). Idaho is the top potato producing state in the U.S. and contributes around 30% of the nation’s potato production. Using the agent-based modeling (ABM) approach, we developed a multi-echelon potato supply chain computer simulation model with six types of agents – seed producers, farmers, shippers, processors, retailers, and logistics companies. In addition to the baseline scenario, we developed two additional climate-related disruption events – drought and snowstorm. We investigated how each of these disruption events impacts different stakeholders across the supply chain in terms of revenue and some other key performance metrics such as lead time, food waste, number of delayed orders, and order fulfillment rate. Results: Extreme climate events not only impact the growers but there is also cascading ripple effects downstream of the supply chain. The methodology developed in this study could be applied to other food and agricultural SC to understand the vulnerabilities of the different links. The findings would potentially help to adopt new policies for the well-being of the overall SC.

54 ENVIRONMENTAL SCIENCES↗

Effect of channel size on sweet potato storage root enlargement in the Tuskegee University hydroponic nutrient film system

The potential of the sweet potato as a food source for future long term manned space missions is being evaluated for NASA's Controlled Ecological Life Support Systems (CELSS) program. Sweet potatoes have been successfully grown in a specially designed Tuskegee University nutrient film technique (TU NFT) system. This hydroponic system yielded storage roots as high as 1790 g/plant fresh weight. In order to determine the effect of channel size on the yield of sweet potatoes, the width and depth of the growing channels were varied in two separate experiments. Widths were studied using the rectangular TU NFT channels with widths of 15 cm (6 in), 30 cm (12 in) and 45 cm (18 in). Channel depths of 5 cm (2 in), 10 cm (4 in), and 15 cm (6 in) were studied using a standard NASA fan shaped Biomass Production Chamber (BPC) channel. A comparison of preliminary results indicated that, except for storage root number, the growth and yield of sweet potatoes were not affected by channel width. Storage root yield was affected by channel depth although storage root number and foliage growth were not. Both experiments are being repeated.

Morris, Carlton E.↗

Growth of potatoes for CELSS

This report summarizes research on the utilization of white potatoes (Solanum tuberosum L.) for space life support systems at the University of Wisconsin-Madison over the period of 1984 to 1993. At full maturity the tuber productivity was 37.5 gm(exp -2) d(exp -1), equating to a growing area requirement for one human (2800 kcal d(exp -1)) of 10.1 m(exp -2). A recirculating nutrient system using slanted trays produced best potato growth and tuber yields when a 2-3 cm layer of gravel or arcillite media was utilized. Potato production was close to maximum under lighting levels of 400 micromol m(exp -2) s(exp -1) of photosynthetic photo flux (PPF) for 24 hours or 800 micromol m(exp -2) s(exp -1) for 12 hours, alternating diurnal temperatures of 22 C and 14 C, relative humidity of 85 percent, and a carbon dioxide level of 1000 micromol m(exp -1). The range of effective concentrations of each separate nutrient is reported. The extensive studies with potatoes in this project have demonstrated that this crop has high productivity of nutritous tubers with a high harvest index in controlled environments, and can fulfill a significant portion of the energy and protein requirements for humans in space.

Tibbitts, T. W.↗

Cultural systems for growing potatoes in space

Higher plants are being evaluated for life support to provide needed food, oxygen and water as well as removal of carbon dioxide from the atmosphere. The successful utilization of plants in space will require the development of not only highly productive growing systems but also highly efficient bioregenerative systems. It will be necessary to recycle all inedible plant parts and all human wastes so that the entire complement of elemental compounds can be reused. Potatoes have been proposed as one of the desirable crops because they are 1) extremely productive, yielding more than 100 metric tons per hectare from field plantings, 2) the edible tubers are high in digestible starch (70%) and protein (10%) on a dry weight basis, 3) up to 80% of the total plant production is in tubers and thus edible, 4) the plants are easily propagated either from tubers or from tissue culture plantlets, 5) the tubers can be utilized with a minimum of processing, and 6) potatoes can be prepared in a variety of different forms for the human diet (Tibbitts et al., 1982). However potatoes have a growth pattern that complicates the development of growing the plants in controlled systems. Tubers are borne on underground stems that are botanically termed 'rhizomes', but in common usage termed 'stolons'. The stolons must be maintained in a dark, moist area with sufficient provision for enlargement of tubers. Stems rapidly terminate in flowers forcing extensive branching and spreading of plants so that individual plants will cover 0.2 m2 or more area. Thus the growing system must be developed to provide an area that is darkened for tuber and root growth and of sufficient size for plant spread. A system developed for growing potatoes, or any plants, in space will have certain requirements that must be met to make them a useful part of a life support system. The system must 1) be constructed of materials, and involve media, that can be reused for many successive cycles of plant growth, 2) involve a minimum quantity of media, 3) contain media that is essentially inert and not oxidize or degrade with use, 4) utilize a recirculating nutrient solution to permit regulation of pH and nutrient concentrations, and 5) be capable of complete automation of all planting, maintenance and harvesting procedures.

NASA Discipline Number 61-10↗

Enhancing physicochemical, bioactive, and nutritional properties of sweet potatoes: Ultrasonic contact drying with slot jet nozzles compared to hot-air drying and freeze drying

Sweet potatoes are a rich source of nutrients and bioactive compounds, but their quality can be impacted by the drying process. This study investigates the impact of slot jet reattachment (SJR) nozzle and ultrasound (US) combined drying (SJR + US) on sweet potato quality, compared to freeze-drying (FD), SJR drying, and hot air drying (HAD). SJR + US drying at 50 °C closely resembled FD in enhancing quality attributes and outperformed HAD and SJR in key areas such as rehydration, shrinkage ratios, and nutritional composition. Notably, SJR + US at 50 °C produced the highest total starch (36.84 g/100 g), total dietary fiber (8.48 g/100 g), total phenolic content (158.19 mg GAE/100 g), total flavonoid content (119.08 mg QE/g), DPPH antioxidant activity (6.44 μmol TE/g), β-carotene (31.98 mg/100 g), and vitamin C (5.27 mg/100 g). It also exhibited higher glass transition temperatures (Tg: 14.49 °C), indicating better stability at room temperature. The hardness values for SJR + US samples were similar to FD, while HAD samples had the highest hardness. SJR + US at 50 °C resulted in the lowest total color changes (ΔE), indicating minimal impact on appearance. Additionally, FTIR analysis revealed that peaks in specific spectral regions indicated superior preservation of bioactive compounds in SJR + US samples compared to other methods, which was also confirmed by principal component analysis (PCA) and heatmap visualization. Overall, these findings suggest that SJR + US is an effective alternative to conventional drying techniques, significantly improving the quality of dried sweet potatoes.

Color↗

Predictive analytics of selections of russet potatoes

We explore the application of machine learning algorithms specifically to enhance the selection process of Russet potato (Solanum tuberosum L.) clones in breeding trials by predicting their suitability for advancement. This study addresses the challenge of efficiently identifying high-yield, disease-resistant, and climate-resilient potato varieties that meet processing industry standards. Leveraging manually collected data from trials in the state of Oregon, we investigate the potential of a wide variety of state-of-the-art binary classification models. The dataset includes 1086 clones, with data on 38 attributes recorded for each clone, focusing on yield, size, appearance, and frying characteristics, with several control varieties planted consistently across four Oregon regions from 2013 to 2021. We conduct a comprehensive analysis of the dataset that includes preprocessing, feature engineering, and imputation to address missing values. We focus on several key metrics such as accuracy, F1-score, and Matthews correlation coefficient (MCC) for model evaluation. The top-performing models, namely a feedforward neural network classifier (Neural Net), a histogram-based gradient boosting classifier (HGBC), and a support vector machine classifier (SVM), demonstrate consistent and significant results. To further validate our findings, we conducted a simulation study using the aims, data-generating mechanisms, estimands, methods, and performance measures (ADEMP) framework, simulating different data-generating scenarios to assess model robustness and performance through true positive, true negative, false positive, and false negative distributions, area under the receiver operating characteristic curve (AUC-ROC) and MCC. The simulation results highlight that non-linear models like SVM and HGBC consistently show higher AUC-ROC and MCC than logistic regression, thus outperforming the traditional linear model across various distributions, and emphasizing the importance of model selection and tuning in agricultural trials. Variable selection further enhances model performance and identifies influential features in predicting trial outcomes. The findings emphasize the potential of machine learning in streamlining the selection process for potato varieties, offering benefits such as increased efficiency, substantial cost savings, and judicious resource utilization. Our study contributes insights into precision agriculture and showcases the relevance of advanced technologies for informed decision-making in breeding programs.

60 APPLIED LIFE SCIENCES↗

Development of POTATO for 2S Module Grading for the CMS Phase-2 Outer Tracker Upgrade

Due to the High Luminosity-Large Hadron Collider (HL-LHC) upgrade, several detectors of the Compact Muon Solenoid (CMS) will need to be replaced, specifically the new Outer Tracker (OT) that will be assembled with 13,200 silicon modules. The OT is composed of two types of modules; the PS (pixel-strip) and the 2S (strip-strip) modules. With a large influx of production of modules, extensive testing is required to ensure quality of the modules. This research introduces POTATO (Phase-II Outer Tracker Analyzer of Test Outputs), a specialized software developed in C++ to analyze, grade, and store results in a centralized database. Module data will be collected from various international production facilities. The implementation of POTATO will facilitate with selecting the best performing modules for the Outer Tracker upgrade. This research is focused on the implementation of the analysis of 2S Module results within POTATO.

Klupshas, Anna↗