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At least 19 records

Development and biodistribution studies of 77 As-labeled trithiol RM2 bioconjugates for prostate cancer: Comparison of [ 77 As]As-trithiol-Ser-Ser-RM2 vs. [ 77 As]As-trithiol-Glu-Ser-RM2

Recent progress with the production of 72 As (2.49 Mev β + max (64%), 3.33 Mev β + max (16%), 834 keV (81%), t 1/2 : 26 h) and 77 As (0.683 Mev β- max (97%), 239 keV (1.59%), t 1/2 : 38.8 h) has facilitated their evaluation as a potential “theranostic pair” for PET imaging and radiotherapy. Our 3 rd generation trithiol chelate with two carboxylic acid groups was further developed as a bifunctional chelate for radioarsenic. The As complex with the trithiol chelate was synthesized and characterized. No carrier added (nca) [ 77 As][H 2 AsO 4 - ] was used for radiolabeling studies. Here, the trithiol chelate was conjugated to the RM2 peptide (DPhe-Gln-Trp-Ala-VaI-Gly-His-Sta-Leu-NH 2 ) via solid phase peptide synthesis with two different linkers, Ser-Ser and Glu-Ser. The trithiol chelate and its RM2 bioconjugates were radiolabeled with nca 77 As, and the RM2 bioconjugates were compared in initial biodistribution studies. The As diacid trithiol complex was characterized by 1 H NMR, 13 C NMR and HR-ESI-MS. The trithiol-RM2 precursor and As trithiol bioconjugates were characterized by HR-ESI-MS and/or LC-ESI-MS. Radiolabeling of the RM2 bioconjugates with 77 As resulted in over 85% radiochemical yield for [ 77 As]As-trithiol-Ser-Ser-RM2 ([ 77 As]8) and 90% for [ 77 As]As-trithiol-Glu-Ser-RM2 ([ 77 As]9). Both radiotracers demonstrated excellent in vitro stability (≥ 90% remaining intact through 24 h in PBS buffer) and were more hydrophilic than previous analogues based on log D 7.4 values. Biodistribution results of the two radiotracers in healthy CF-1 male mice demonstrated blockable pancreatic uptake at 1 h (82% for ([ 77 As]8 and 78% for [ 77 As]9) indicating specific gastrin-releasing peptide receptor (GRPR) uptake. The primary route of excretion was through the gastrointestinal system for both radiotracers. A new trithiol chelate with improved hydrophilicity was successfully conjugated to the RM2 peptide via two linkers, and high radiolabeling yield with nca 77 As was achieved. In vivo biodistribution studies with both radiotracers demonstrated blockable pancreatic uptake suggestive of specific receptor uptake.

07 ISOTOPE AND RADIATION SOURCES↗

Reference Model 2 Scaled Geometry (RM2: River Current Turbine)

Contains the Reference Model 2 (RM2) scaled scale geometry files of the River Current Turbine, developed by the Reference Model Project (RMP). These scaled geometry files are saved as SolidWorks assembly, IGS, and STEP files, and require a CAD program to view. The scaled RM2 device was tested at the Saint Anthony Falls Laboratory (SAFL) at the University of Minnesota flume. The scale of the geometries included in this submission are at a 1:15 scale compared to the full scale geometry. This data was generated upon completion of the project on September 30, 2014. The Reference Model Project (RMP), sponsored by the U.S. Department of Energy (DOE), was a partnered effort to develop open-source MHK point designs as reference models (RMs) to benchmark MHK technology performance and costs, and an open-source methodology for design and analysis of MHK technologies, including models for estimating their capital costs, operational costs, and levelized costs of energy. The point designs also served as open-source test articles for university researchers and commercial technology developers. The RMP project team, led by Sandia National Laboratories (SNL), included a partnership between DOE, three national laboratories, including the National Renewable Energy Laboratory (NREL), Pacific Northwest National Laboratory (PNNL), and Oak Ridge National Laboratory (ORNL), the Applied Research Laboratory of Penn State University, and Re Vision Consulting. Reference Model 2 (RM2) is a variable speed dual-rotor cross-flow river turbine that is deployed at the water?s surface. It was designed for deployment at a reference site modeled after a reach in the Mississippi River near Baton Rouge, Louisiana. The rotors are anchored to a two-pontoon vessel platform. Surface deployment of the turbine minimizes the handling requirements during deployment and recovery and reduces overall costs for all O&M activities, including allowing for easy access to the power conversion chain (PCC). The design (two rotors per platform) also reduces the environmental footprint and associated environmental compliance costs.

16 TIDAL AND WAVE POWER↗

Reference Model 2 Cost Breakdown (RM2: River Current Turbine)

Contains the Reference Model 2 (RM2) spreadsheets with the cost breakdown structure (CBS) for the levelized cost of energy (LCOE) calculations for a single RM2 device and multiple unit arrays. These spreadsheets are contained within an XLSX file and a spreadsheet editor such as Microsoft Excel is needed to open the file. This data was generated upon completion of the project on September 30, 2014. The Reference Model Project (RMP), sponsored by the U.S. Department of Energy (DOE), was a partnered effort to develop open-source MHK point designs as reference models (RMs) to benchmark MHK technology performance and costs, and an open-source methodology for design and analysis of MHK technologies, including models for estimating their capital costs, operational costs, and levelized costs of energy. The point designs also served as open-source test articles for university researchers and commercial technology developers. The RMP project team, led by Sandia National Laboratories (SNL), included a partnership between DOE, three national laboratories, including the National Renewable Energy Laboratory (NREL), Pacific Northwest National Laboratory (PNNL), and Oak Ridge National Laboratory (ORNL), the Applied Research Laboratory of Penn State University, and Re Vision Consulting. Reference Model 2 (RM2) is a variable speed dual-rotor cross-flow river turbine that is deployed at the water?s surface. It was designed for deployment at a reference site modeled after a reach in the Mississippi River near Baton Rouge, Louisiana. The rotors are anchored to a two-pontoon vessel platform. Surface deployment of the turbine minimizes the handling requirements during deployment and recovery and reduces overall costs for all O&M activities, including allowing for easy access to the power conversion chain (PCC). The design (two rotors per platform) also reduces the environmental footprint and associated environmental compliance costs.

16 TIDAL AND WAVE POWER↗

U.S. Department of Energy Reference Model Program RM2: Experimental Results

The Reference Model Project (RMP), sponsored by the U.S. Department of Energy’s (DOE) Wind and Water Power Technologies Program within the Office of Energy Efficiency & Renewable Energy (EERE), aims at expediting industry growth and efficiency by providing non-proprietary Reference Models (RM) of MHK technology designs as study objects for open-source research and development (Neary et al. 2014a,b). As part of this program, MHK turbine models were tested in a large open channel facility at the University of Minnesota’s St. Anthony Falls Laboratory (UMN - SAFL) . Reference Model 2 (RM2) is a 1:15 geometric scale dual - rotor cross flow vertical axis device with counter - rotating rotors, each with a rotor diameter d T = 0.43m and rotor height, h T = 0.323 m. RM2 is a river turbine designed for a site modeled after a reach in the lower Mississippi River near Baton Rouge, Louisiana (Barone et al. 2014) . Precise blade angular position and torque measurements were synchronized with three acoustic Doppler velocimeters (ADV) aligned with each rotor and the midpoint for RM2 . Flow conditions for each case were controlled such that depth, h = 1m, and volumetric flow rate, Q w = 2. 35m 3 s -1 , resulting in a hub height velocity of approximately U hub = 1. 2 ms -1 and blade chord length Reynolds numbers of Re c = 6 .1x10 4 . Vertical velocity profiles collected in the wake of each device from 1 to 10 rotor diameters are used to estimate the velocity recovery and turbulent characteristics in the wake, as well as the interaction of the counter-rotating rotor wakes. The development of this high resolution laboratory investigation provides a robust dataset that enables assessing computational fluid dynamics (CFD) models and their ability to accurately simulate turbulent inflow environments, device performance metrics, and to reproduce wake velocity deficit, recovery and higher order turbulent statistics.

13 HYDRO ENERGY↗

RM2: Transform operations

The two-dimensional transform used in the research TV source encoder, RM2 is discussed. It is shown that both conceptually and in terms of the number of required computations, the RM2 transform is considerably simpler than the Fast Hadamard Transform. The latter can in fact be generated by extending the RM2 transform.

Rice, R. F.↗

Reference Model 2 Full Scale Geometry (RM2: River Current Turbine)

Contains the Reference Model 2 (RM2) full scale geometry files of the River Current Turbine, developed by the Reference Model Project (RMP). These full scale geometry files are saved as SolidWorks assembly, IGS, X_T, and STEP files, and require a CAD program to view. This data was generated upon completion of the project on September 30, 2014. The Reference Model Project (RMP), sponsored by the U.S. Department of Energy (DOE), was a partnered effort to develop open-source MHK point designs as reference models (RMs) to benchmark MHK technology performance and costs, and an open-source methodology for design and analysis of MHK technologies, including models for estimating their capital costs, operational costs, and levelized costs of energy. The point designs also served as open-source test articles for university researchers and commercial technology developers. The RMP project team, led by Sandia National Laboratories (SNL), included a partnership between DOE, three national laboratories, including the National Renewable Energy Laboratory (NREL), Pacific Northwest National Laboratory (PNNL), and Oak Ridge National Laboratory (ORNL), the Applied Research Laboratory of Penn State University, and Re Vision Consulting. Reference Model 2 (RM2) is a variable speed dual-rotor cross-flow river turbine that is deployed at the waters surface. It was designed for deployment at a reference site modeled after a reach in the Mississippi River near Baton Rouge, Louisiana. The rotors are anchored to a two-pontoon vessel platform. Surface deployment of the turbine minimizes the handling requirements during deployment and recovery and reduces overall costs for all O&M activities, including allowing for easy access to the power conversion chain (PCC). The design (two rotors per platform) also reduces the environmental footprint and associated environmental compliance costs.

16 TIDAL AND WAVE POWER↗

RM2: rms error comparisons

The root-mean-square error performance measure is used to compare the relative performance of several widely known source coding algorithms with the RM2 image data compression system. The results demonstrate that RM2 has a uniformly significant performance advantage.

Rice, R. F.↗

Present-day plate motions

A data set comprising 110 spreading rates, 78 transform fault azimuths and 142 earthquake slip vectors was inverted to yield a new instantaneous plate motion model, designated RM2. The mean averaging interval for the relative motion data was reduced to less than 3 My. A detailed comparison of RM2 with angular velocity vectors which best fit the data along individual plate boundaries indicates that RM2 performs close to optimally in most regions, with several notable exceptions. On the other hand, a previous estimate (RM1) failed to satisfy an extensive set of new data collected in the South Atlantic Ocean. It is shown that RM1 incorrectly predicts the plate kinematics in the South Atlantic because the presently available data are inconsistent with the plate geometry assumed in deriving RM1. It is demonstrated that this inconsistency can be remedied by postulating the existence of internal deformation with the Indian plate, although alternate explanations are possible.

Minster, J. B.↗

Present-day plate motions

An instantaneous plate-motion model, Relative Motion 2 (RM2), is obtained by inverting a data set comprising 110 spreading rates, 78 transform fault azimuths, and 142 earthquake slip vectors. RM2 is compared with angular velocity vectors which best fit the data along individual plate boundaries and, while the model performs close to optimally in most regions, attention is directed to those regions which are not suitably described by the model. Reasons for the discrepancies between RM2 and observations for the India-Antarctica plate boundary, the Pacific-India plate boundary, and the east-west trending transform fault azimuths observed in the French-American Mid-Ocean Undersea Study area are discussed.

Minster, J. B.↗

Geophysical interpretation of satellite laser ranging measurements of crustal movement in California

As determined by satellite laser ranging the rate of contraction of a 900 kilometer baseline between sites located near Quincy in northern California and San Diego in southern California is about 61 to 65 mm/yr with a formal uncertainty of about 10 mm/yr. The measured changes in baseline length are a manifestation of the relative motion between the North America and Pacific tectonic plates. This long baseline result is compared to measurements made by more conventional means on shorter baselines. Additional information based on seismicity, geology, and theoretical modelling is also analyzed. Deformation lying within a few tens of kilometers about the major faults in southern California accounts for most, but not all of the observed motion. Further motion is attributable to a broader scale deformation in southern California. Data suggesting crustal movements north of the Garlock fault, in and near the southern Sierra Nevada and local motion at an observatory are also critically reviewed. The best estimates of overall motion indicated by ground observations lie between 40 and 60 mm/yr. This lies within one or two standard deviations of that deduced by satellite ranging but the possibility of some unresolved deficit cannot be dismissed. The long time scale RM2 plate tectonic model of Minster and Jordan predicts a contraction between 47 and 53 mm/yr depending on the extension rate of the Basin and Range. Thus the ground based observations, satellite laser ranging (SLR) results, and RM2 rates differ at about the 10 mm/yr level and are consistent with one another within the data and model uncertainties.

Cohen, S. C.↗

B2, B7 or B10: Which palm-based blend mandate wise to be chosen in Malaysia?

The diminishing fossil energy resources, coupled with heightened interest in the abatement of greenhouse gas emissions and concerns about energy security have motivated Malaysia to produce palm-based biodiesel and it has been started to be exported since 2006. In line with this issue, the government in Malaysia launched the palm-based biodiesel blending mandate of five percent (B5) in the federal administration of Putrajaya on 1{sup st} June 2011. This was then followed by four states: Malacca on July 11, Negeri Sembilan on August 1, Kuala Lumpur on September 1 and Selangor on October 1 of the same year but it is yet to be implemented nationwide. However what is the wise blend mandate to be chosen? Thus, this paper seeks to examine the possible impact of various blend mandates implementation (B2, B7 and B10) on the palm oil industry market variables (stock and price) since the main aim of biodiesel industry in Malaysia is to reduce domestic palm oil stock to below one million tones and provide a floor price to support Crude Palm Oil (CPO) prices at RM2,000 per tonne. A structural econometric model consisting of nine structural equations and three identities was proposed in this study. The model has been estimated by two stage least squares (2SLS) method using annual data for the period 1976-2013. The study indicates that counterfactual simulation of a decrease from B5 to B2 predicts a decrease (11.2 per cent) in CPO domestic consumption for biodiesel usage, 731.02 per cent reduction in CPO stock and an increase of 27.41 percent in domestic price of CPO. However the increase in the blend mandate from B5 to B7 and B10 suggest that domestic consumption of CPO for biodiesel purpose increase 7.40 and 18.55 percent respectively. The interesting findings in this study suggest that no matter whether Malaysian government increase or decrease the blend mandate the increase in the price of CPO are the same with an increase of is 27.41 percent. Hence, this study suggests that the lower blend mandate is the most favourable on.+-e compared to increasing it in this short period due to high production cost of CPO prices. However, future study on economic feasibility of palm-based biodiesel has to be conducted to support this study.

29 ENERGY PLANNING, POLICY, AND ECONOMY↗

U.S. Department of Energy Reference Model Program RM1: Experimental Results

The Reference Model Project (RMP), sponsored by the U.S. Department of Energy’s (DOE) Wind and Water Power Technologies Program within the Office of Energy Efficiency & Renewable Energy (EERE), aims at expediting industry growth and efficiency by providing non-proprietary Reference Models (RM) of MHK technology designs as study objects for open-source research and development (Neary et al. 2014a,b). As part of this program, MHK turbine models were tested in a large open channel facility at the University of Minnesota’s St. Anthony Falls Laboratory (UMN-SAFL). Reference Model 1 (RM2) is a 1:40 geometric scale dual-rotor axial flow horizontal axis device with counter-rotating rotors, each with a rotor diameter d T = 0.5m. Precise blade angular position and torque measurements were synchronized with three acoustic Doppler velocimeters (ADVs) aligned with each rotor and the midpoint for RM1. Flow conditions for each case were controlled such that depth, h = 1m, and volumetric flow rate, Q w = 2.425m3s -1 , resulting in a hub height velocity of approximately U hub = 1.05ms -1 and blade chord length Reynolds numbers of R ec ≈ 3.0x105. Vertical velocity profiles collected in the wake of each device from 1 to 10 rotor diameters are used to estimate the velocity recovery and turbulent characteristics in the wake, as well as the interaction of the counter-rotating rotor wakes. The development of this high resolution laboratory investigation provides a robust dataset that enables assessing turbulence performance models and their ability to accurately predict device performance metrics, including computational fluid dynamics (CFD) models that can be used to predict turbulent inflow environments, reproduce wake velocity deficit, recovery and higher order turbulent statistics, as well as device performance metrics.

16 TIDAL AND WAVE POWER↗

An advanced imaging communication system for planetary exploration

An advanced imaging communication system (AICS) for planetary exploration is presented. The system offers 'end-to-end' information rate improvements of 3 to 5 times over existing systems in addition to extensive user flexibility to adapt his rate/fidelity priorities to fit a particular mission. AICS contains two major system elements. The first is a concatenated Reed-Solomon/Viterbi coded channel. This provides a powerful, yet practical, solution to the usual 'error vulnerability' problem associated with compressed data. The second major element is an extremely adaptive image data compression algorithm called RM2. The details of this algorithm as presently simulated are discussed in considerable detail. Used in conjunction with the virtually error free performance of the Reed-Solomon/Viterbi channel the stated AICS advantages are obtained.

Rice, R. F.↗

A concept for dynamic control of RPV information system parameters

A globally adaptive image compression structure has been developed for use in the tactical environment of a remotely piloted vehicle. The control structure is based on an image compression algorithm RM2 but can be easily extended to standard algorithms. It is shown that this structure provides an operator with the flexibility to dynamically maximize the usefulness of a limited and changing bit rate and should significantly improve overall system performance in tactical environments.

Rice, R. F.↗

Viscoelastic deformation near active plate boundaries

Model deformations near the active plate boundaries of Western North America using space-based geodetic measurements as constraints are discussed. The first six months of this project were spent gaining familarity with space-based measurements, accessing the Crustal Dynamics Data Information Computer, and building time independent deformation models. The initial goal was to see how well the simplest elastic models can reproduce very long base interferometry (VLBI) baseline data. From the Crustal Dynamics Data Information Service, a total of 18 VLBI baselines are available which have been surveyed on four or more occasions. These data were fed into weighted and unweighted inversions to obtain baseline closure rates. Four of the better quality lines are illustrated. The deformation model assumes that the observed baseline rates result from a combination of rigid plate tectonic motions plus a component resulting from elastic strain build up due to a failure of the plate boundary to slip at the full plate tectonic rate. The elastic deformation resulting from the locked plate boundary is meant to portray interseismic strain accumulation. During and shortly after a large interplate earthquake, these strains are largely released, and points near the fault which were previously retarded suddenly catch up to the positions predicted by rigid plate models. Researchers judge the quality of fit by the sum squares of weighted residuals, termed total variance. The observed baseline closures have a total variance of 99 (cm/y)squared. When the RM2 velocities are assumed to model the data, the total variance increases to 154 (cm/y)squared.

Ward, S. N.↗