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At least 19 records

The Arthromitus stage of Bacillus cereus: intestinal symbionts of animals

In the guts of more than 25 species of arthropods we observed filaments containing refractile inclusions previously discovered and named "Arthromitus" in 1849 by Joseph Leidy [Leidy, J. (1849) Proc. Acad. Nat. Sci. Philadelphia 4, 225-233]. We cultivated these microbes from boiled intestines of 10 different species of surface-cleaned soil insects and isopod crustaceans. Literature review and these observations lead us to conclude that Arthromitus are spore-forming, variably motile, cultivable bacilli. As long rod-shaped bacteria, they lose their flagella, attach by fibers or fuzz to the intestinal epithelium, grow filamentously, and sporulate from their distal ends. When these organisms are incubated in culture, their life history stages are accelerated by light and inhibited by anoxia. Characterization of new Arthromitus isolates from digestive tracts of common sow bugs (Porcellio scaber), roaches (Gromphodorhina portentosa, Blaberus giganteus) and termites (Cryptotermes brevis, Kalotermes flavicollis) identifies these flagellated, spore-forming symbionts as a Bacillus sp. Complete sequencing of the 16S rRNA gene from four isolates (two sow bug, one hissing roach, one death's head roach) confirms these as the low-G+C Gram-positive eubacterium Bacillus cereus. We suggest that B. cereus and its close relatives, easily isolated from soil and grown on nutrient agar, enjoy filamentous growth in moist nutrient-rich intestines of healthy arthropods and similar habitats.

Non-NASA Center

Clear air turbulence as a response to meso- and synoptic-scale dynamic processes

The paper presents both the Roach equation for the rate of energy dissipation due to clear air turbulence and Richardson number tendencies in isentropic coordinates and examines the implications of these formulations to determine whether there is a dynamic interdependence between Ri and the nonturbulent deformation processes. The equation representing the ln(Ri) tendency is applied diagnostically to grids from an isentropic analysis of archived soundings. The evolution of the Richardson number fields over 12-hour time periods is examined using a mechanistic model. It is suggested that the application of the Roach equation for the turbulent dissipation rate should have a more restricted use. Analyses of the meso-alpha scale Richardson number and of the Richardson number tendency fields reveal a phase relationship consistent with the theoretical predictions.

Keller, John L.

Performance Analysis of a Hardware Implemented Complex Signal Kurtosis Radio-Frequency Interference Detector

Radio-frequency interference (RFI) is a known problem for passive remote sensing as evidenced in the L-band radiometers SMOS, Aquarius and more recently, SMAP. Various algorithms have been developed and implemented on SMAP to improve science measurements. This was achieved by the use of a digital microwave radiometer. RFI mitigation becomes more challenging for microwave radiometers operating at higher frequencies in shared allocations. At higher frequencies larger bandwidths are also desirable for lower measurement noise further adding to processing challenges. This work focuses on finding improved RFI mitigation techniques that will be effective at additional frequencies and at higher bandwidths. To aid the development and testing of applicable detection and mitigation techniques, a wide-band RFI algorithm testing environment has been developed using the Reconfigurable Open Architecture Computing Hardware System (ROACH) built by the Collaboration for Astronomy Signal Processing and Electronics Research (CASPER) Group. The testing environment also consists of various test equipment used to reproduce typical signals that a radiometer may see including those with and without RFI. The testing environment permits quick evaluations of RFI mitigation algorithms as well as show that they are implementable in hardware. The algorithm implemented is a complex signal kurtosis detector which was modeled and simulated. The complex signal kurtosis detector showed improved performance over the real kurtosis detector under certain conditions. The real kurtosis is implemented on SMAP at 24 MHz bandwidth. The complex signal kurtosis algorithm was then implemented in hardware at 200 MHz bandwidth using the ROACH. In this work, performance of the complex signal kurtosis and the real signal kurtosis are compared. Performance evaluations and comparisons in both simulation as well as experimental hardware implementations were done with the use of receiver operating characteristic (ROC) curves. The complex kurtosis algorithm has the potential to reduce data rate due to onboard processing in addition to improving RFI detection performance.

microwaves

Performance Analysis of a Hardware Implemented Complex Signal Kurtosis Radio-Frequency Interference Detector

Radio-frequency interference (RFI) is a known problem for passive remote sensing as evidenced in the L-band radiometers SMOS, Aquarius and more recently, SMAP. Various algorithms have been developed and implemented on SMAP to improve science measurements. This was achieved by the use of a digital microwave radiometer. RFI mitigation becomes more challenging for microwave radiometers operating at higher frequencies in shared allocations. At higher frequencies larger bandwidths are also desirable for lower measurement noise further adding to processing challenges. This work focuses on finding improved RFI mitigation techniques that will be effective at additional frequencies and at higher bandwidths. To aid the development and testing of applicable detection and mitigation techniques, a wide-band RFI algorithm testing environment has been developed using the Reconfigurable Open Architecture Computing Hardware System (ROACH) built by the Collaboration for Astronomy Signal Processing and Electronics Research (CASPER) Group. The testing environment also consists of various test equipment used to reproduce typical signals that a radiometer may see including those with and without RFI. The testing environment permits quick evaluations of RFI mitigation algorithms as well as show that they are implementable in hardware. The algorithm implemented is a complex signal kurtosis detector which was modeled and simulated. The complex signal kurtosis detector showed improved performance over the real kurtosis detector under certain conditions. The real kurtosis is implemented on SMAP at 24 MHz bandwidth. The complex signal kurtosis algorithm was then implemented in hardware at 200 MHz bandwidth using the ROACH. In this work, performance of the complex signal kurtosis and the real signal kurtosis are compared. Performance evaluations and comparisons in both simulation as well as experimental hardware implementations were done with the use of receiver operating characteristic (ROC) curves.

radiometers

Semidirect calculation of steady two- and three-dimensional flows

This paper describes a semidirect method for rapidly solving steady-state viscous flows described by the complete Navier-Stokes equations. The current results are for two-dimensional incompressible flows in general channels at arbitrary Reynolds numbers, but work in progress on compressible and three-dimensional flows is also described. The basic concept of semidirect methods is to use the recently developed fast (direct, or noniterative) linear solvers to solve linearized equations, which are then iterated to solve the nonlinearity. The method used here is an extension of the Split NOS method (Roache, 1975)

Roache, P. J.

A search for forward scattering of sunlight from lunar libration clouds

Data obtained with the white-light coronagraph on Skylab are analyzed to determine the radiance of forward scattered sunlight from particles in lunar libration regions. It is found that the libration regions could not be distinguished against the solar K + F coronal background, and upper limits are determined for the libration cloud radiance as well as density enhancements in the libration region. The actual spatial density enhancement is calculated on the basis of previous observations by Roach (1975). The radiance contrast of a possible model libration cloud is calculated with respect to the K- and F-corona/zodiacal-light background and is found to be a maximum in the vicinity of a solar elongation angle of the order of 30 deg.

Munro, R. H.

Case studies of clear air turbulence using the diagnostic Richardson Number Tendency formulation

The results of four case studies of clear air turbulence (CAT) using the diagnostic Richardson number tendency (DRT) formulation are highlighted. The performance of this technique in resolving regions of documented CAT encounters is encouraging. Its operational adaptability appears particularly attractive in that input data can be supplied by the currently operational rawinsonde system. Two CAT indices are calculated deterministically, sensing synoptic scale changes in static stability and vertical wind shear conductive for supporting mesoscale CAT layers. These two indices reveal volumes of the troposphere which act as source regions for patches of CAT. The first, the time to reach the critical Richardson number necessary for the initiation of turbulent conditions, was first used by Oard (1974). A second index is devised which is an adaptation of Roach's (1970) work relating synoptic scale and mesoscale energetical coupling and gives more information on the relative intensity of these source regions. The output from the DRT computer module highlights specific regions of the atmosphere which can be interpreted operationally in terms of a CAT encounter probability.

Keller, J. L.

Development of an operational specific CAT risk (SCATR) index

The original formulations of Roach (1970) and Oard (1974) for the calculation of clear air turbulence (CAT) potential from synoptic scale data were extended. An index which gives a measure of the specific risk of encountering CAT - the specific clear air turbulence risk (SCATR) index - was defined. This index takes into account both the locally and advected contributions to the energy necessary for CAT. The advected contribution is associated with the role of atmospheric gravity waves. The SCATR index was calculated for a number of cases where documented encounters with CAT occurred. Of particular interest were those made for cases involving severe CAT. The results for the two severe CAT cases run were quite impressive and elicited considerable interest from operational aviation meteorologists.

Keller, J. L.

Project Explorer takes its second step: GAS-608 in engineering development

An a continuation of its Project Explorer series, the Alabama Space and Rocket Center is sponsoring the development of two additional Get Away Special payloads. Details are given of GAS-608, including descriptions of its six experiments in organic crystal growth, roach eggs, yeast, radish seeds, bacterial morphology, and silicon crystals. A brief summary is also presented of GAS-105 and the Space Camp program for stimulating student first hand participation in space flight studies. GAS-608 will carry six student experiments, which will involve biology, crystal growth, and biochemistry in addition to a centralized package for electronics and power supply.

Kitchens, Philip H.

A simple method for simulating gasdynamic systems

A simple method for performing digital simulation of gasdynamic systems is presented. The approach is somewhat intuitive, and requires some knowledge of the physics of the problem as well as an understanding of the finite difference theory. The method is explicitly shown in appendix A which is taken from the book by P.J. Roache, 'Computational Fluid Dynamics,' Hermosa Publishers, 1982. The resulting method is relatively fast while it sacrifices some accuracy.

Hartley, Tom T.

RDBE Development and Progress

A digital backend based on the ROACH board has been developed jointly by the National Radio Astronomy Observatory and MIT Haystack Observatory. The RDBE will have both Polyphase Filterbank and Digital Downconverter personalities. The initial configuration outputs sixteen 32-MHz channels, comprised of half the channels from the PFB processing of the two IF inputs, for use in the VLBI2010 geodetic system and in the VLBA sensitivity upgrade project. The output rate is 2x109 bits/second (1x10(exp 9) bits/sec = 1 Gbps) over a 10 GigE connection to the Mark 5C with the data written in Mark 5B format on disk.

Niell, A.

Reynolds-Averaged Turbulence Model Assessment for a Highly Back-Pressured Isolator Flowfield

The use of computational fluid dynamics in scramjet engine component development is widespread in the existing literature. Unfortunately, the quantification of model-form uncertainties is rarely addressed with anything other than sensitivity studies, requiring that the computational results be intimately tied to and calibrated against existing test data. This practice must be replaced with a formal uncertainty quantification process for computational fluid dynamics to play an expanded role in the system design, development, and flight certification process. Due to ground test facility limitations, this expanded role is believed to be a requirement by some in the test and evaluation community if scramjet engines are to be given serious consideration as a viable propulsion device. An effort has been initiated at the NASA Langley Research Center to validate several turbulence closure models used for Reynolds-averaged simulations of scramjet isolator flows. The turbulence models considered were the Menter BSL, Menter SST, Wilcox 1998, Wilcox 2006, and the Gatski-Speziale explicit algebraic Reynolds stress models. The simulations were carried out using the VULCAN computational fluid dynamics package developed at the NASA Langley Research Center. A procedure to quantify the numerical errors was developed to account for discretization errors in the validation process. This procedure utilized the grid convergence index defined by Roache as a bounding estimate for the numerical error. The validation data was collected from a mechanically back-pressured constant area (1 2 inch) isolator model with an isolator entrance Mach number of 2.5. As expected, the model-form uncertainty was substantial for the shock-dominated, massively separated flowfield within the isolator as evidenced by a 6 duct height variation in shock train length depending on the turbulence model employed. Generally speaking, the turbulence models that did not include an explicit stress limiter more closely matched the measured surface pressures. This observation is somewhat surprising, given that stress-limiting models have generally been developed to better predict shock-separated flows. All of the models considered also failed to properly predict the shape and extent of the separated flow region caused by the shock boundary layer interactions. However, the best performing models were able to predict the isolator shock train length (an important metric for isolator operability margin) to within 1 isolator duct height.

Baurle, Robert A.

From TPL assessment to design optimization: Wave energy converter control co-design applied to the RM3

The Reference Model (RM) project developed six marine energy converter concepts using a sequential design methodology, which, while widely adopted in the industry, often overlooks interactions between system components, resulting in suboptimal designs. One such example is the Reference Model 3 (RM3), a two-body point absorber wave energy converter (WEC). An assessment using the Technology Performance Level (TPL) revealed that RM3’s low power-to-cost ratio, partly due to expensive steel construction, limits its techno-economic performance. Here, this study aims to redesign RM3 by reducing its scale and employing control co-design to integrate WEC and Power Take-Off (PTO) dynamics, constraints, and cost considerations within an optimization framework. We demonstrate the limitations of RM3’s current PTO design and explore the benefits of scaling down to enhance techno-economic viability by lowering material costs. Using WecOptTool, we conduct a parameter sweep over gear ratios and spring stiffnesses for various Commercial Off-The-Shelf generators in irregular wave conditions. Our findings emphasize the importance of aligning PTO components with WEC dynamics, showing that control co-design and strategic scaling can improve RM3’s power-to-cost ratio. This study presents a transferable example of applied control co-design for other WECs, supporting early-stage developers in their design decisions.

Off the shelf components

A photometric study of the counterglow from space.

Photometric observations of the region of the counterglow (Gegenschein) made from OSO-6 are examined. The observations were made during the September to October 1970 period when the counterglow was between the Milky Way arms at a relatively high (negative) galactic latitude. The lines of sight included a slice across the antisun region at an inclination of 48 degrees to the ecliptic. A comparison is made between the photometric gradients as measured from the spacecraft and similar gradients deduced from ground-based observations.

Roach, F. E.

A photometric perturbation of the counterglow.

An example of photometric variability of a counterglow region in the antisolar direction is discussed. A speculative interpretation of its significance leads to the suggestion that the weakened radiance of the counterglow may in this case be due to a dust cloud of scattering material in the general region of the earth-moon system.

Roach, F. E.

Modeling Orifice Pulse Tube Coolers

We have developed a calculational model that treats all the components of an orifice pulse tube cooler. We base our analysis on 1-dimensional thermodynamic equations for the regenerator and we assume that all mass flows, pressure oscillations and temperature oscillations are small and sinusoidal. Non-linear pressure drop effects are included in the regenerator to account for finite pressure amplitude effects. The resulting mass flows and pressures are matched at the boundaries with the other components of the cooler: compressor, aftercooler, cold heat exchanger, pulse tube, hot heat exchanger, orifice and reservoir. The results of the calculation are oscillating pressures, mass flows and enthalpy flows in the main components of the cooler. By comparing with the calculations of other available models, we show that our model is very similar to REGEN 3 from NIST and DeltaE from Los Alamos National Lab. Our model is much easier to use than other available models because of its simple graphical interface and the fact that no guesses are required for the operating pressures or mass flows. In addition, the model only requires a few minutes of running time allowing many parameters to be optimized in a reasonable time. A version of the model is available for use over the World Wide Web at http://irtek.arc.nasa.gov. Future enhancements include adding a bypass orifice and including second order terms in steady mass streaming and steady heat transfer. A two-dimensional anelastic approximation of the fluid equations will be used as the basis for the latter analysis. Preliminary results are given in dimensionless numbers appropriate for oscillating compressible flows. The model shows how transverse heat transfer reduces enthalpy flow, particularly for small pulse tubes. The model also clearly shows mass recirculation in the open tube on the order of the tube length. They result from the higher order Reynolds stresses. An interesting result of the linearized approach is that the steady mass streaming does not affect the enthalpy flow at second order. The major effect of recirculating mass streaming is to increase transverse temperature gradients, which leads to higher entropy production and reduced efficiency.

Roach, Kittel P.

Monodomain Liquid‐Crystal Elastomer Lattices for Broad Strain‐Rate Mechanical Damping

Designing structures that effectively dissipate energy across a broad range of mechanical loading rates, including those from compression, shock, and vibration, poses a significant engineering challenge. In this study, liquid‐crystal elastomers (LCEs), which possess anisotropic properties due to the alignment of their polymer backbone, are explored. As a result, LCEs exhibit a soft elastic response under mechanical loading, making them ideal for energy dissipation. Advances in additive manufacturing (AM) enable simple fabrication of foamlike dissipative structures with complex lattice geometries. Herein, direct ink write 3D printing, an extrusion AM method, is used to fabricate aligned, monodomain LCE lattice structures for broad strain‐rate mechanical damping. In this work, it is shown that these structures can dissipate strain energy in quasi‐static environments, comparable to traditional elastomeric lattices, and provide improved damping under high strain‐rate drop testing due to LCE soft elasticity. Additionally, under dynamic mechanical vibration, monodomain LCE lattices enhance damping at structural natural frequencies and provide high‐frequency attenuation. Finally, in these findings, a promising method is presented for fabricating mechanical damping devices that effectively dissipate energy across a broad range of loading rates.

3D printings

Digital Light Process 3D Printing of Magnetically Aligned Liquid Crystalline Elastomer Free–forms

Liquid crystalline elastomers (LCEs) are anisotropic soft materials capable of large dimensional changes when subjected to a stimulus. The magnitude and directionality of the stimuli-induced thermomechanical response is associated with the alignment of the LCE. Recent reports detail the preparation of LCEs by additive manufacturing (AM) techniques, predominately using direct ink write printing. Another AM technique, digital light process (DLP) 3D printing, has generated significant interest as it affords LCE free-forms with high fidelity and resolution. However, one challenge of printing LCEs using vat polymerization methods such as DLP is enforcing alignment. Here, we document the preparation of aligned, main-chain LCEs via DLP 3D printing using a 100 mT magnetic field. Systematic examination isolates the contribution of magnetic field strength, alignment time, and build layer thickness on the degree of orientation in 3D printed LCEs. Informed by this fundamental understanding, DLP is used to print complex LCE free-forms with through-thickness variation in both spatial orientations. Further, the hierarchical variation in spatial orientation within LCE free-forms is used to produce objects that exhibit mechanical instabilities upon heating. DLP printing of aligned LCEs opens new opportunities to fabricate stimuli-responsive materials in form factors optimized for functional use in soft robotics and energy absorption.

36 MATERIALS SCIENCE