Serum-lipoprotein distribution and protein analysis by refractometry
Serum-lipoprotein distribution and protein analysis by refractometry
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Serum-lipoprotein distribution and protein analysis by refractometry
A method of determining the refractive and absorptive properties of optically transparent materials involves a combination of theoretical and experimental analysis of electromagnetic responses of whispering-gallery-mode (WGM) resonator disks made of those materials. The method was conceived especially for use in studying transparent photorefractive materials, for which purpose this method affords unprecedented levels of sensitivity and accuracy. The method is expected to be particularly useful for measuring temporally varying refractive and absorptive properties of photorefractive materials at infrared wavelengths. Still more particularly, the method is expected to be useful for measuring drifts in these properties that are so slow that, heretofore, the properties were assumed to be constant. The basic idea of the method is to attempt to infer values of the photorefractive properties of a material by seeking to match (1) theoretical predictions of the spectral responses (or selected features thereof) of a WGM of known dimensions made of the material with (2) the actual spectral responses (or selected features thereof). Spectral features that are useful for this purpose include resonance frequencies, free spectral ranges (differences between resonance frequencies of adjacently numbered modes), and resonance quality factors (Q values). The method has been demonstrated in several experiments, one of which was performed on a WGM resonator made from a disk of LiNbO3 doped with 5 percent of MgO. The free spectral range of the resonator was approximately equal to 3.42 GHz at wavelengths in the vicinity of 780 nm, the smallest full width at half maximum of a mode was approximately equal to 50 MHz, and the thickness of the resonator in the area of mode localization was 30 microns. In the experiment, laser power of 9 mW was coupled into the resonator with an efficiency of 75 percent, and the laser was scanned over a frequency band 9 GHz wide at a nominal wavelength of approximately equal to 780 nm. Resonance frequencies were measured as functions of time during several hours of exposure to the laser light. The results of these measurements, plotted in the figure, show a pronounced collective frequency drift of the resonator modes. The size of the drift has been estimated to correspond to a change of 8.5 x 10(exp -5) in the effective ordinary index of refraction of the resonator material.
This work proposes a standard reference material (SRM) for measurements of refractive index in the infrared spectral region (1–12 μm). The purpose of this material is to enable cross-method comparison of refractive index data obtained through minimum deviation, ellipsometry, interferometry, and prism coupling techniques. This will allow vendors and metrology laboratories, regardless of which technique they employ, to certify that their measurements meet required levels of accuracy, precision, and repeatability. Drawing on foundational work by Tilton at the National Bureau of Standards, a figure of merit for candidate SRM materials is outlined, and three materials are selected for further evaluation. Ultimately, barium fluoride is proposed as the most suitable SRM for the infrared spectral region, based on its broad transmission range, chemical and mechanical stability, low temperature dependence of refractive index, and well-characterized dispersion behavior.
We measured the densities of the cell sap, endoplasm and cell wall of Nitellopsis obtusa and Chara corallina using interference microscopy, refractometry, immersion refractometry, equilibrium sedimentation and chemical microanalysis techniques. These values are important for the determination of many rheological properties of the cytoplasm as well as for understanding buoyancy regulation, dispersal mechanisms and how cells respond to gravity. The average densities of the cell sap, endoplasm and cell wall are 1,006.9, 1,016.7 and 1,371 kg m-3 for Nitellopsis and 1,005.0, 1,013.9, and 1,355.3 kg m-3 for Chara.
The 1.2 meter telescope system was built for the Goddard Space Flight Center (GSFC) in 1973-74 by the Kollmorgen Corporation as a highly accurate tracking telescope. The telescope is an azimuth-elevation mounted six mirror Coude system. The facility has been used for a wide range of experimentation including helioseismology, two color refractometry, lunar laser ranging, satellite laser ranging, visual tracking of rocket launches, and most recently satellite and aircraft streak camera work. The telescope is a multi-user facility housed in a two story dome with the telescope located on the second floor above the experimenter's area. Up to six experiments can be accommodated at a given time, with actual use of the telescope being determined by the location of the final Coude mirror. The telescope facility is currently one of the primary test sites for the Crustal Dynamics Network's new UNIX based telescope controller software, and is also the site of the joint Crustal Dynamics Project / Photonics Branch two color research into atmospheric refraction.
In this study we measured the optical characteristics of normally white twisted nematic (NWTN) and super twisted nematic (STN ) cells. Though no dynamic computer model was available, the static observations were compared with computer simulated behavior. The measurements were taken as a function of both viewing angle and applied voltage and included in the static case not only luminance but also contrast ratio and chromaticity . We employed the computer model Twist Cell Optics, developed at Kent State in conjunction with this study, and whose optical modeling foundation, Iike the ViDEOS program, is the 4 x 4 matrix method of Berreman. In order to resolve discrepancies between the experimental and modeled data the optical parameters of the individual cell components, where not known, were determined using refractometry, profilometry, and various forms of ellipsometry. The resulting agreement between experiment and model is quite good due primarily to a better understanding of the structure and optics of dichroic sheet polarizers. A description of the model and test cells employed are given in section 2. Section 3 contains the experimental data gathered and section 4 gives examples of the fit between model and experiment. Also included with this report are a pair of papers which resulted from the research and which detail the polarizer properties and some of the cell characterization methods.
Topics covered include: Instrument for Analysis of Greenland's Glacier Mills Cryogenic Moisture Apparatus; A Transportable Gravity Gradiometer Based on Atom Interferometry; Three Methods of Detection of Hydrazines; Crossed, Small-Deflection Energy Analyzer for Wind/Temperature Spectrometer; Wavefront Correction for Large, Flexible Antenna Reflector; Novel Micro Strip-to-Waveguide Feed Employing a Double-Y Junction; Thin-Film Ferro Electric-Coupled Microstripline Phase Shifters With Reduced Device Hysteresis; Two-Stage, 90-GHz, Low-Noise Amplifier; A 311-GHz Fundamental Oscillator Using InP HBT Technology; FPGA Coprocessor Design for an Onboard Multi-Angle Spectro-Polarimetric Imager; Serrating Nozzle Surfaces for Complete Transfer of Droplets; Turbomolecular Pumps for Holding Gases in Open Containers; Triaxial Swirl Injector Element for Liquid-Fueled Engines; Integrated Budget Office Toolbox; PLOT3D Export Tool for Tecplot; Math Description Engine Software Development Kit; Astronaut Office Scheduling System Software; ISS Solar Array Management; Probabilistic Structural Analysis Program; SPOT Program; Integrated Hybrid System Architecture for Risk Analysis; System for Packaging Planetary Samples for Return to Earth; Offset Compound Gear Drive; Low-Dead-Volume Inlet for Vacuum Chamber; Simple Check Valves for Microfluidic Devices; A Capillary-Based Static Phase Separator for Highly Variable Wetting Conditions; Gimballing Spacecraft Thruster; Finned Carbon-Carbon Heat Pipe with Potassium Working Fluid; Lightweight Heat Pipes Made from Magnesium; Ceramic Rail-Race Ball Bearings; Improved OTEC System for a Submarine Robot; Reflector Surface Error Compensation in Dual-Reflector Antennas; Enriched Storable Oxidizers for Rocket Engines; Planar Submillimeter-Wave Mixer Technology with Integrated Antenna; Widely Tunable Mode-Hop-Free External-Cavity Quantum Cascade Laser; Non-Geiger-Mode Single-Photon Avalanche Detector with Low Excess Noise; Using Whispering-Gallery-Mode Resonators for Refractometry; RF Device for Acquiring Images of the Human Body; Reactive Collision Avoidance Algorithm; Fast Solution in Sparse LDA for Binary Classification; Modeling Common-Sense Decisions in Artificial Intelligence; Graph-Based Path-Planning for Titan Balloons; Nanolaminate Membranes as Cylindrical Telescope Reflectors; Air-Sea Spray Airborne Radar Profiler Characterizes Energy Fluxes in Hurricanes; Large Telescope Segmented Primary Mirror Alignment; and Simplified Night Sky Display System.
Matthews (1992) has proposed that HCN "polymer" is ubiquitous in the solar system. We apply vacuum deposition and spectroscopic techniques previously used on synthetic organic heteropolymers (tholins), kerogens, and meteoritic organic residues to the measurement of the optical constants of poly-HCN in the wavelength range 0.05-40 micrometers. These measurements allow quantitative comparison with spectrophotometry of organic-rich bodies in the outer solar system. In a specific test of Matthews' hypothesis, poly-HCN fails to match the optical constants of the haze of the Saturnian moon, Titan, in the visible and near-infrared derived from astronomical observations and standard models of the Titan atmosphere. In contrast, a tholin produced from a simulated Titan atmosphere matches within the probable errors. Poly-HCN is much more N-rich than Titan tholin.