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At least 19 records

Shear stress stimulates phosphorylation of eNOS at Ser(635) by a protein kinase A-dependent mechanism

Shear stress stimulates nitric oxide (NO) production by phosphorylating endothelial NO synthase (eNOS) at Ser(1179) in a phosphoinositide-3-kinase (PI3K)- and protein kinase A (PKA)-dependent manner. The eNOS has additional potential phosphorylation sites, including Ser(116), Thr(497), and Ser(635). Here, we studied these potential phosphorylation sites in response to shear, vascular endothelial growth factor (VEGF), and 8-bromocAMP (8-BRcAMP) in bovine aortic endothelial cells (BAEC). All three stimuli induced phosphorylation of eNOS at Ser(635), which was consistently slower than that at Ser(1179). Thr(497) was rapidly dephosphorylated by 8-BRcAMP but not by shear and VEGF. None of the stimuli phosphorylated Ser(116). Whereas shear-stimulated Ser(635) phosphorylation was not affected by phosphoinositide-3-kinase inhibitors wortmannin and LY-294002, it was blocked by either treating the cells with a PKA inhibitor H89 or infecting them with a recombinant adenovirus-expressing PKA inhibitor. These results suggest that shear stress stimulates eNOS by two different mechanisms: 1) PKA- and PI3K-dependent and 2) PKA-dependent but PI3K-independent pathways. Phosphorylation of Ser(635) may play an important role in chronic regulation of eNOS in response to mechanical and humoral stimuli.

Non-NASA Center↗

Surface-Enhanced Raman Spectroscopy (SERS) for Venus Atmospheric Characterization and Trace Organics Identification

The chemical composition of Venus’ clouds has attracted considerable attention since the first atmospheric probes entered the planet in the early 1980s. We know from these early studies that cloud aerosols consist of micron and submicron droplets of sulfuric acid (~85%) and water (~15%), but outstanding questions still exist regarding their trace chemical composition, especially the identity of the unknown UV absorber(s) and the possible presence of organic material (Spacek & Benner, 2021). Veritas and DaVinci will launch at the end of the decade to advance our understanding of Venus’ surface and atmosphere, however these missions are not designed to sample and analyze cloud aerosols at trace levels. Raman spectroscopy is an ideal candidate for characterizing aerosols and has a track record in studies of Earth’s atmosphere. Raman provides broad chemical screening of organic and inorganic molecules within seconds, and Raman instrumentation can be miniaturized to meet the stringent size, weight, and power (SWaP) constraints of an atmospheric probe while retaining sensitivity and specificity that allow spectral fingerprinting of compounds and functional groups. Raman alone is insufficient to characterize trace constituents however, as limits of detection are typically in the 10s to 100s of ppm at best for planetary Raman spectrometers. Surface-Enhanced Raman Spectroscopy (SERS), a method that utilizes nanoscale materials interacting with a sample to enhance weak Raman signals by orders of magnitude when probed by a laser, has the potential to drastically improve the sensitivity of a Raman instrument while adding little-to-no SWaP nor complexity to a scientific payload. We aim to de-risk SERS technology for rapid infusion in future planetary science missions by developing custom SERS substrates tailored towards Venus atmospheric exploration. We have developed SERS substrates consisting of nanotextured silver films and tested them on potential Venus-relevant organic compounds, with preliminary results indicating sub-ppm sensitivity. Additionally, we have planned tests to investigate durability, longevity, and other key characteristics that impact SERS substrate performance to prepare SERS technology for flight.

Venus↗

The Spacecraft Emergency Response System (SERS) for Autonomous Mission Operations

Today, most mission operations are geared toward lowering cost through unmanned operations. 7-day/24-hour operations are reduced to either 5-day/8-hour operations or become totally autonomous, especially for deep-space missions. Proper and effective notification during a spacecraft emergency could mean success or failure for an entire mission. The Spacecraft Emergency Response System (SERS) is a tool designed for autonomous mission operations. The SERS automatically contacts on-call personnel as needed when crises occur, either on-board the spacecraft or within the automated ground systems. Plus, the SERS provides a group-ware solution to facilitate the work of the person(s) contacted. The SERS is independent of the spacecraft's automated ground system. It receives and catalogues reports for various ground system components in near real-time. Then, based on easily configurable parameters, the SERS determines whom, if anyone, should be alerted. Alerts may be issued via Sky-Tel 2-way pager, Telehony, or e-mail. The alerted personnel can then review and respond to the spacecraft anomalies through the Netscape Internet Web Browser, or directly review and respond from the Sky-Tel 2-way pager.

Breed, Julia↗

Enhancing SERS by Means of Supramolecular Charge Transfer

In a proposed method of sensing small quantities of molecules of interest, surface enhanced Raman scattering (SERS) spectroscopy would be further enhanced by means of intermolecular or supramolecular charge transfer. There is a very large potential market for sensors based on this method for rapid detection of chemical and biological hazards. In SERS, the Raman signals (vibrational spectra) of target molecules become enhanced by factors of the order of 108 when those molecules are in the vicinities of nanostructured substrate surfaces that have been engineered to have plasmon resonances that enhance local electric fields. SERS, as reported in several prior NASA Tech Briefs articles and elsewhere, has remained a research tool and has not yet been developed into a practical technique for sensing of target molecules: this is because the short range (5 to 20 nm) of the field enhancement necessitates engineering of receptor molecules to attract target molecules to the nanostructured substrate surfaces and to enable reliable identification of the target molecules in the presence of interferants. Intermolecular charge-transfer complexes have been used in fluorescence-, photoluminescence-, and electrochemistry-based techniques for sensing target molecules, but, until now, have not been considered for use in SERS-based sensing. The basic idea of the proposed method is to engineer receptor molecules that would be attached to nanostructured SERS substrates and that would interact with the target molecules to form receptor-target supramolecular charge-transfer complexes wherein the charge transfer could be photoexcited.

Wong, Eric↗

Evidence for a Broad Relativistic Iron Line from the Neutron Star LMXB Ser X-1

We report on an analysis of XMM-Newton data from the neutron star low mass X-ray binary (LMXB) Serpens X-1 (Ser X-1). Spectral analysis of EPIC PN data indicates that the previously known broad iron Ka emission line in this source has a significantly skewed structure with a moderately extended red wing. The asymmetric shape of the line is well described with the laor and diskline models in XSPEC, which strongly supports an inner accretion disk origin of the line. To our knowledge this is the first strong evidence for a relativistic line in a neutron star LMXB. This finding suggests that the broad lines seen in other neutron star LMXBs likely originate from the inner disk as well. Detailed study of such lines opens up a new way to probe neutron star parameters and their strong gravitational fields. The laor model describes the line from Ser X-1 somewhat better than diskline, and suggests that the inner accretion disk radius is less than 6GM/c(exp 2). This is consistent with the weak magnetic fields of LMXBs, and may point towards a high compactness and rapid spin of the neutron star. Finally, the inferred source inclination angle in the approximate range 50-60 deg is consistent with the lack of dipping from Ser X-1.

Bhattacharyya, Sudip↗

Electride Mediated Surface Enhanced Raman Scattering (SERS)

An electride may provide surface enhanced Raman scattering (SERS). The electride, a compound where the electrons serve as anions, may be a ceramic electride, such as a conductive ceramic derived from mayenite, or an organic electride, for example. The textured electride surface or electride particles may strongly enhance the Raman scattering of organic or other Raman active analytes. This may also provide a sensitive method for monitoring the chemistry and electronic environment at the electride surface. The results are evidence of a new class of polariton (i.e., a surface electride-polariton resonance mechanism) that is analogous to the surface plasmon-polariton resonance that mediates conventional SERS.

Anderson, Mark S.↗

Simultaneous IR and X-ray burst observation of Ser X-1

It has been suggested that the very bright IR bursts observed from the Rapid Burster (MXB 1730-335) are due to Type I X-ray bursts. If the suggestion is correct, similar bright IR bursts may, in general, accompany Type I X-ray bursts. This paper investigates this possibility for one specific case. During the 1977 coordinated worldwide burst watch, simultaneous X-ray and IR observations were made. On 00 h 00 min 25s June 17 UT, a Type I X-ray burst was observed, which was almost certainly emitted by Ser X-1 (MXB 1837+05, 4U1837+04). Simultaneous IR observations failed to detect any bursts.

Lewin, W. H. G.↗

Identification of Ser-543 as the major regulatory phosphorylation site in spinach leaf nitrate reductase

Spinach leaf NADH:nitrate reductase (NR) responds to light/dark signals and photosynthetic activity in part as a result of rapid regulation by reversible protein phosphorylation. We have identified the major regulatory phosphorylation site as Ser-543, which is located in the hinge 1 region connecting the cytochrome b domain with the molybdenum-pterin cofactor binding domain of NR, using recombinant NR fragments containing or lacking the phosphorylation site sequence. Studies with NR partial reactions indicated that the block in electron flow caused by phosphorylation also could be localized to the hinge 1 region. A synthetic peptide (NR6) based on the phosphorylation site sequence was phosphorylated readily by NR kinase (NRk) in vitro. NR6 kinase activity tracked the ATP-dependent inactivation of NR during several chromatographic steps and completely inhibited inactivation/phosphorylation of native NR in vitro. Two forms of NRk were resolved by using anion exchange chromatography. Studies with synthetic peptide analogs indicated that both forms of NRk had similar specificity determinants, requiring a basic residue at P-3 (i.e., three amino acids N-terminal to the phosphorylated serine) and a hydrophobic residue at P-5. Both forms are strictly calcium dependent but belong to distinct families of protein kinases because they are distinct immunochemically.

NASA Discipline Plant Biology↗

Aqueously Dispersed Silver Nanoparticle-Decorated Boron Nitride Nanosheets for Reusable, Thermal Oxidation-Resistant Surface Enhanced Raman Spectroscopy (SERS) Devices

The impurity-free aqueous dispersions of boron nitride nanosheets (BNNS) allowed the facile preparation of silver (Ag) nanoparticle-decorated BNNS by chemical reduction of an Ag salt with hydrazine in the presence of BNNS. The resultant Ag-BNNS nanohybrids remained dispersed in water, allowing convenient subsequent solution processing. By using substrate transfer techniques, Ag-BNNS nanohybrid thin film coatings on quartz substrates were prepared and evaluated as reusable surface enhanced Raman spectroscopy (SERS) sensors that were robust against repeated solvent washing. In addition, because of the unique thermal oxidation-resistant properties of the BNNS, the sensor devices may be readily recycled by short-duration high temperature air oxidation to remove residual analyte molecules in repeated runs. The limiting factor associated with the thermal oxidation recycling process was the Ostwald ripening effect of Ag nanostructures.

Lin, Yi↗

Material for surface-enhanced Raman spectroscopy, and SER sensors and method for preparing same

Metal-doped sol-gel materials, suitable for use as sensors for surface-enhanced Raman spectroscopic analysis for trace chemical detection, are produced by effecting gelation and solvent removal of a doped sol-gel under mild temperature conditions. At least in certain instances reaction and drying will desirably be effected in an oxygen-starved environment. The metal of the sol-gel material functions, when irradiated, to produce a plasmon field for interaction with molecules of an analyte in contact therewith, increasing by orders of magnitude Raman photons that are generate by excitation radiation, and the method allows matching of the metal and metal particle size to a wavelength of light (or incident radiation, e.g., laser radiation) to generate surface plasmons. The porosity of the sol-gel material dramatically increases the surface area, and thereby the amount of metal exposed for analyte interaction. The sensors provided may be in the form of glass vials, fiber optics, multi-well micro-sample plates, etc., having surface coatings of the doped sol-gel material, to provide sampling systems for use in a Raman instrument.

Farquharson, Stuart↗

Enabling Advanced Automation in Spacecraft Operations with the Spacecraft Emergency Response System

True autonomy is the Holy Grail of spacecraft mission operations. The goal of launching a satellite and letting it manage itself throughout its useful life is a worthy one. With true autonomy, the cost of mission operations would be reduced to a negligible amount. Under full autonomy, any problems (no matter the severity or type) that may arise with the spacecraft would be handled without any human intervention via some combination of smart sensors, on-board intelligence, and/or smart automated ground system. Until the day that complete autonomy is practical and affordable to deploy, incremental steps of deploying ever-increasing levels of automation (computerization of once manual tasks) on the ground and on the spacecraft are gradually decreasing the cost of mission operations. For example, NASA's Goddard Space Flight Center (NASA-GSFC) has been flying spacecraft with low cost operations for several years. NASA-GSFC's SMEX (Small Explorer) and MIDEX (Middle Explorer) missions have effectively deployed significant amounts of automation to enable the missions to fly predominately in 'light-out' mode. Under light-out operations the ground system is run without human intervention. Various tools perform many of the tasks previously performed by the human operators. One of the major issues in reducing human staff in favor of automation is the perceived increased in risk of losing data, or even losing a spacecraft, because of anomalous conditions that may occur when there is no one in the control center. When things go wrong, missions deploying advanced automation need to be sure that anomalous conditions are detected and that key personal are notified in a timely manner so that on-call team members can react to those conditions. To ensure the health and safety of its lights-out missions, NASA-GSFC's Advanced Automation and Autonomy branch (Code 588) developed the Spacecraft Emergency Response System (SERS). The SERS is a Web-based collaborative environment that enables secure distributed fault and resource management. The SERS incorporates the use of intelligent agents, threaded discussions, workflow, database connectivity, and links to a variety of communications devices (e.g., two-way paging, PDA's, and Internet phones) via commercial gateways. When the SERS detects a problem, it notifies on-call team members, who then can remotely take any necessary actions to resolve the anomalies.The SERS goes well beyond a simple '911' system that sends out an error code to everyone with a pager. Instead, SERS' software agents send detailed data (i.e., notifications) to the most appropriate team members based on the type and severity of the anomaly and the skills of the on-call team members. The SERS also allows the team members to respond to the notifications from their wireless devices. This unique capability ensures rapid response since the team members no longer have to go to a PC or the control center for every anomalous event. Most importantly, the SERS enables safe experimentation with various techniques for increasing levels of automation, leading to robust autonomy. For the MIDEX missions at NASA GSFC, the SERS is used to provide 'human-in-the-loop' automation. During lights-out operations, as greater control is given to the MIDEX automated systems, the SERS can be configured to page remote personnel and keep them informed regarding actions taking place in the control center. Remote off-duty operators can even be given the option of enabling or inhibiting a specific automated response in near real time via their two-way pagers. The SERS facilitates insertion of new technology to increase automation, while maintaining the safety and security of mission resources. This paper will focus on SERS' overall functionality and how SERS has been designed to handle the monitoring and emergency response for missions with varying levels of automation. The paper will also convey some of the key lessons learned from SERS' deployment across of variety of missions, highlighting this incremental approach to achieving 'robust autonomy'.

Breed, Julie↗

New Material for Surface-Enhanced Raman Spectroscopy

A chemical method of synthesis and application of coating materials that are especially suitable for surface-enhanced Raman spectroscopy (SERS) has been developed. The purpose of this development is to facilitate the utilization of the inherently high sensitivity of SERS to detect chemicals of interest (analytes) in trace amounts, without need for lengthy sample preparation. Up to now, the use of SERS has not become routine because the methods available have not been able to reproduce sampling conditions and provide quantitative measurements. In contrast, the coating materials of the present method enable analysis with minimum preparation of samples, and SERS measurements made using these materials are reproducible and reversible. Moreover, unlike in methods investigated in prior efforts to implement SERS, sampling is not restricted to such specific environments as electrolytes or specific solvents. The coating materials of this method are porous glasses, formed in sol-gel processes, that contain small particles of gold or silver metal. Materials of this type can be applied to the sample-contact surfaces of a variety of sampling and sensing devices, including glass slides, glass vials, fiber-optic probes, and glass tubes. Glass vials with their insides coated according to this method are particularly convenient for SERS to detect trace chemicals in solutions: One simply puts a sample solution containing the analyte(s) into a vial, then puts the vial into a Raman spectrometer for analysis. The chemical ingredients and the physical conditions of the sol-gel process have been selected so that the porous glass formed incorporates particles of the desired metal with size(s) to match the wavelength(s) of the SERS excitation laser in order to optimize the generation of surface plasmons. The ingredients and processing conditions have further been chosen to tailor the porosity and polarity of the glass to optimize the sample flow and the interaction between the analyte(s) and the plasmon field that generates Raman photons. The porous silica network of a sol-gel glass creates a unique environment for stabilizing SERS-active metal particles. Relative to other material structures that could be considered for SERS, the porous silica network offers higher specific surface area and thus greater interaction between analyte molecules and metal particles. Efforts to perform SERS measurements with the help of sampling devices coated by this method have been successful. In tests, numerous organic and inorganic chemicals were analyzed in several solvents, including water. The results of the tests indicate that the SERS measurements were reproducible within 10 percent and linear over five orders of magnitude. One measure of the limits of detectability of chemicals in these tests was found to be a concentration of 300 parts per billion. Further development may eventually make it possible to realize the full potential sensitivity of SERS for detecting some analytes in quantities as small as a single molecule.

Farquharson, Stuart↗

An Optimization Approach to Support Science Decision Making for Lunar Surface Exploration

Introduction: Scientific exploration is one of the three pillars of NASA’s Moon2Mars architecture, with crew surface extra vehicular activities (EVA) serving a critical enabling function. Development of surface EVA operational planning and execution, specifically integrating science and flight control teams (FCT), is currently being explored through analog scenarios. This integration, exercised, for example, through the Joint EVA and Hu-man Surface Mobility Test Team (JETT), allows for science input on EVA activities in near real-time through a Science Evaluation Room (SER), or Arte-mis science backroom, which integrates with the broader FCT through the Science Officer. The SER works within the FCT to support dynamic EVA planning in response to changes in operational constraints as well as science opportunities and re-prioritization, increasing the mission science return and accelerating the accomplishment of the Moon2Mars science objectives. The SER works within the FCT to provide recommendations to traverse execution in near real-time. One challenge is the requirement to deliver SER inputs to the FCT on operationally relevant timelines. Failure to do so may result in suboptimal execution of science exploration EVAs or even loss of key science objectives. To close this gap, we present a network optimization tool to allow the SER to provide rapid input to the FCT in response to changes in operational constraints or science opportunities. Inputs are predicated on approved science objectives, and clear rationale must be provided to the FCT for any requested change. Accordingly, this tool incorporates the Science Traceability Matrix (STM), SER prioritization scheme, and station characterization and action planning with operational constraints such as duration, traverse speed, and distance to maximize science objectives based on SER priorities, consistent with FCT operational requirements. Method: As a proof of concept, we used an existing linear programing software package used to simulate optimal routes through cellular metabolism. We built a Demonstrative Model with three STM objectives and four stations on a region of the Moon. The objectives were given an arbitrary prioritization and mapped to the stations through four possible crew actions. (Figs. 1 and 2). This station to STM mapping is consistent with the method used by the JETT5 Science Team to develop analog surface EVA science planning. We used a grid system with the landing site at the origin and the four stations placed across the positive x,y quadrant. Actions were assigned to each station and the accomplishment of those actions resulted in a numerical “reward” based on the ability of that action to achieve science objectives. The aggregate reward from each individual STM objective contributes to a global score (Science Yield), weighted by its priority. Operational constraints included a requirement to start and end at the landing site, 5 minutes each for initial station characterization and “clean up,” and variable total EVA time, traverse rate (fixed to 0.5 meters per second in our example), and time to perform each action (10, 5, 7, and 15 min for actions 1, 2, 3, and 4, respectively). Additional constraints and variables will be added in the future (e.g., sample mass, number of stations, traverse route constraints, illumination). Optimization. We converted the connections (arcs) between these stations (nodes) into a mixed integer linear programming optimization problem (arcs = constraints, nodes = variables) with the objective to maximize Science Yield. For any action, the Science Yield is equal to the relevance of that action to an STM objective [3, 2, and 1 point(s) for High, Med., and Low relevance, respectively], multiplied by the STM Objective Priority [3, 2, and 1 point(s) for High, Med., and Low priority, respectively]. This resulted in a model that computes the optimal station and action combination to maximize the Science Yield. These weightings can be adjusted by the SER as desired. Results: We explored three test cases for the Demonstrative Model. First, we set the maximum EVA duration to 120 minutes and computed the optimal route (Fig. 3A). The model suggested per-forming Actions 1 and 2 at Station P01, followed by Actions 1 and 2 at Station P02, and finally Actions 1 and 3 at Station P04 before returning to the Landing Site. Second, we adjusted the STM Objective Priori-ty order and computed the new optimal route (Fig. 3B). Under this situation, the model suggested per-forming all Actions at Station P02 followed by all Actions at Station P03. The previous test cases were relevant to SER planning activities. Next, we explored providing mid-EVA replanning input to the FCT. Scenario: While executing the Route in Fig. 3A the crew finishes at Station P01 and FCT decides that the EVA needs to finish in 45 minutes back at the Landing Site. FCT asks SER to recommend changes to the plan to accommodate this operation-al change. Using the model and incorporating these new constraints (start at Station P01, max. time of 45 min), the model suggested performing Actions 2 and 4 at Station P03 (Fig. 4), requiring 41 minutes to complete and return to the Landing Site. Interestingly, Station 3 was not part of the original route. Using the model, we determined the EVA would need 66 minutes, instead of 45, in order for the original Station P04 to yield a larger Science Yield than Station P03. The parametrization and simulation was per-formed in less than a minute, demonstrating the operational relevance of the approach. Future Efforts: The results from the Demonstrative Model suggest this tool can accelerate SER decision making on operationally relevant timelines. Use in analog activities, such as JETT5 or follow-ons, which have over a dozen stations for a crew to explore and over a dozen actions per station, will provide needed validation of the utility of this tool for planning EVAs, replanning mid-EVA, or planning follow-on EVAs based on previous results. Further integration with FCT execution monitoring tools may provide additional efficiency gains, al-lowing rapid and iterative exploration of operation-al and science decision space by the FCT and SER.

Science Operations↗

Absorption Cross-Sections of Ozone in the Ultraviolet and Visible Spectral Regions: Status report 2015

The activity Absorption Cross-Sections of Ozone (ACSO) started in 2008 as a joint initiative of the International Ozone Commission (IO3C), the World Meteorological Organization (WMO) and the IGACO (Integrated Global Atmospheric Chemistry Observations) O3/UV subgroup to study, evaluate, and recommend the most suitable ozone absorption cross-section laboratory data to be used in atmospheric ozone measurements. The evaluation was basically restricted to ozone absorption cross-sections in the UV range with particular focus on the Huggins band. Up until now, the data of Bass and Paur published in 1985 (BP, 1985) are still officially recommended for such measurements. During the last decade it became obvious that BP (1985) cross-section data have deficits for use in advanced space-borne ozone measurements. At the same time, it was recognized that the origin of systematic differences in ground-based measurements of ozone required further investigation, in particular whether the BP (1985) cross-section data might contribute to these differences. In ACSO, different sets of laboratory ozone absorption cross-section data (including their dependence on temperature) of the group of Reims (France) (Brion et al., 1993, 1998, 1992, 1995, abbreviated as BDM, 1995) and those of Serdyuchenko et al. (2014), and Gorshelev et al. (2014), (abbreviated as SER, 2014) were examined for use in atmospheric ozone measurements in the Huggins band. In conclusion, ACSO recommends:(a) The spectroscopic data of BP (1985) should no longer be used for retrieval of atmospheric ozone measurements.(b) For retrieval of ground-based instruments of total ozone and ozone profile measurements by the Umkehr method performed by Brewer and Dobson instruments data of SER (2014) are recommended to be used. When SER (2014) is used, the difference between total ozone measurements of Brewer and Dobson instruments are very small and the difference between Dobson measurements at AD and CD wavelength pairs are diminished.(c) For ground-based Light Detection and Ranging (LIDAR) measurements the use of BDM (1995) or SER (2014) is recommended.(d) For satellite retrieval the presently widely used data of BDM (1995) should be used because SER (2014) seems less suitable for retrievals that use wavelengths close to 300 nm due to a deficiency in the signal-to-noise ratio in the SER (2014) dataset.The work of ACSO also showed: The need to continue laboratory cross-section measurements of ozone of highest quality. The importance of careful characterization of the uncertainties of the laboratory measurements. The need to extend the scope of such studies to other wavelength ranges (particularly to cover not only the Huggins band but also the comparison with the mid-infrared region). The need for regular cooperation of experts in spectral laboratory measurements and specialists in atmospheric (ozone) measurements.

Orphal, Johannes↗

Symbol error rate performance evaluation of the LM37 multimegabit telemetry modulator-demodulator unit

The LM37 multimegabit telemetry modulator-demodulator unit was tested for evaluation of its symbol error rate (SER) performance. Using an automated test setup, the SER tests were carried out at various symbol rates and signal-to-noise ratios (SNR), ranging from +10 to -10 dB. With the aid of a specially designed error detector and a stabilized signal and noise summation unit, measurement of the SER at low SNR was possible. The results of the tests show that at symbol rates below 20 megasymbols per second (MS)s) and input SNR above -6 dB, the SER performance of the modem is within the specified 0.65 to 1.5 dB of the theoretical error curve. At symbol rates above 20 MS/s, the specification is met at SNR's down to -2 dB. The results of the SER tests are presented with the description of the test setup and the measurement procedure.

Malek, H.↗

Versatile Vials

Advanced Fuel Research, Inc. (AFR) developed a new sol-gel material coating for sample vials to be used in surface-enhanced Raman (SER) spectroscopy. In the fields of biomedical and forensic science, the coated vials are used to identify physiological chemicals present in blood and urine. The vials are used in the pharmaceutical industry in the area of drug development and discovery. In the drug enforcement field, using the vials helps with detection of trace amounts of drugs on surfaces. The SER vials will also help to identify contaminants in water sources, such as various inorganics and pesticides. The new vials have advantages over other SER materials. Former products required that samples be pre-mixed or dissolved into specific solvents or reagents. The SER vials do not have these limitations, and measurements can be performed using any solvent. Furthermore, the signal appears in less than one second. The sol-gel material will stay active for at least one year and the vials are compatible with any Raman spectrometer. Most importantly, the SER activity can be duplicated from vial-to-vial, allowing for reproducible results.

Source record↗

Performance results of Grumman prototype Space Station Space Erectable Radiator System ground test articles

The paper addresses individual-radiator performance results of the prototype Space Erectable Radiator System (SERS) in both ambient and thermal vacuum environments. The radiator design utilizing a two-phase fluid loop is outlined, along with SERS design requirements, radiator panel hardware, and whiffletree clamp hardware providing a dry-contact interface of the SERS panel with the heat exchanger of the thermal bus. It is observed that throughout integrated thermal-bus tests, SERS panels managed the load demands whether interfacing with twin condensers, in parallel-flow configuration, or with shear-flow condensers, in a cross-flow configuration. It is found that the insulation losses in the integrated and stand-alone test points are approximately 2 to 6 pct. The motorized whiffletree clamp is seen as performing satisfactorily during remote operations as well as maintaining 28,800-lb force throughout the test duration without requiring further adjustments.

Gisondo, Francine↗

Immunoassay readout method using extrinsic Raman labels adsorbed on immunogold colloids

An immunoassay readout method based on surface-enhanced Raman scattering (SERS) is described. The method exploits the SERS-derived signal from reporter molecules that are coimmobilized with biospecific species on gold colloids. This concept is demonstrated in a dualanalyte sandwich assay, in which two different antibodies covalently bound to a solid substrate specifically capture two different antigens from an aqueous sample. The captured antigens in turn bind selectively to their corresponding detection antibodies. The detection antibodies are conjugated with gold colloids that are labeled with different Raman reporter molecules, which serve as extrinsic labels for each type of antibody. The presence of a specific antigen is established by the characteristic SERS spectrum of the reporter molecule. A near-infrared diode laser was used to excite efficiently the SERS signal while minimizing fluorescence interference. We show that, by using different labels with little spectral overlap, two different antigenic species can be detected simultaneously. The potential of this concept to function as a readout strategy for multiple analytes is briefly discussed.

NASA Program Advanced Human Support Technology↗