SPL 7 and SPL 8 represent a novel flowering regulation mechanism in switchgrass
Summary The aging pathway in flowering regulation is controlled mainly by micro RNA 156 (miR156). Studies in Arabidopsis thaliana reveal that nine miR156‐targeted SQUAMOSA PROMOTER BINDING ‐ LIKE ( SPL ) genes are involved in the control of flowering. However, the roles of SPL s in flowering remain elusive in grasses. Inflorescence development in switchgrass was characterized using scanning electron microscopy ( SEM ). Microarray, quantitative reverse transcription polymerase chain reaction (q RT ‐ PCR ), chromatin immunoprecipitation (Ch IP )‐ PCR and EMSA were used to identify regulators of phase transition and flowering. Gene function was characterized by downregulation and overexpression of the target genes. Overexpression of SPL 7 and SPL 8 promotes flowering, whereas downregulation of individual genes moderately delays flowering. Simultaneous downregulation of SPL 7 / SPL 8 results in extremely delayed or nonflowering plants. Furthermore, downregulation of both genes leads to a vegetative‐to‐reproductive reversion in the inflorescence, a phenomenon that has not been reported in any other grasses. Detailed analyses demonstrate that SPL 7 and SPL 8 induce phase transition and flowering in grasses by directly upregulating SEPALLATA3 ( SEP 3 ) and MADS 32 . Thus, the SPL 7 / 8 pathway represents a novel regulatory mechanism in grasses that is largely different from that in Arabidopsis . Additionally, genetic modification of SPL 7 and SPL8 results in much taller plants with significantly increased biomass yield and sugar release.