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Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

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At least 19 records
All sourcesNASA NTRSDOE OSTI
DOE OSTI2025

UDP-glucuronic acid decarboxylase in alfalfa: a target to improve ruminal digestibility of stems

Alfalfa (Medicago sativa) has a high nutritional value, but poor digestibility of the stems limits its value as an energy source in ruminant diets. Xylan and lignin negatively affect cell wall digestibility, whereas pectins have high digestibility in the rumen. In plants, UDP-xylose synthase (UXS) catalyses the decarboxylation of UDP-glucuronic acid to form UDP-xylose in an irreversible step that is key for xylan synthesis. Here, we functionally characterized two UXS genes in alfalfa, namely MsaUXS2 and MsaUXS4, and investigated their impact on ruminal digestibility. Both genes are more highly expressed in stems than leaves, and the enzymes have UDP-glucuronic acid decarboxylase activity in vitro. Silencing of MsaUXS2 and MsaUXS4 via RNAi altered plant growth and resulted in a 40% decrease in xylose, a 115% increase in arabinose, and a 60% increase in galacturonic acid in the polysaccharide matrix as well as a 20% decrease in lignin in the cell wall. Together, our results show a major role for UXS2 and UXS4 in xylan synthesis and secondary cell wall deposition in alfalfa. Additionally, in vitro rumen digestibility assays for the silenced lines had on average 30% increased gas production at 24 h, demonstrating the potential of targeting UXS genes to increase stem digestibility.

UDP-xylose synthase↗
DOE OSTI2024

Alfalfa

Alfalfa-based testbeds enable building equipment, control products, and workforce development tools to interact with dynamic building simulations representing the desired building, system, weather, and grid configuration. Alfalfa is used to de-risk implementation of load flexibility prior to field deployment, reducing the costs and timelines associated with adoption of decarbonization technology at the grid edge.

building energy modeling↗
DOE OSTI2024

Alfalfa Virtual Building Service: Software Engineering Best Practices Applied to Runtime Interaction with Building Energy Models

Buildings are active participants in increasingly complex energy systems. Building Energy Modeling (BEM) has a key role to play in planning and de-risking an equitable energy transition, with BEM-backed "virtual buildings" critical path for diverse applications that include workforce training tools, Hardware-in-the-Loop (HIL) experimentation to study equipment performance under a range of conditions, Control-Hardware-in-the-Loop (CHIL) experimentation to de-risk commercial control implementations at equipment through grid orchestration levels, and integration of dynamic load profiles into grid modeling tools for energy system experimentation at the urban scale. Modeling requirements vary across these applications, but many software engineering tasks do not. The Alfalfa Virtual Building Service (AVBS, see https://github.com/NREL/alfalfa/wiki) is an open-source web service that solves these common tasks robustly in one place, providing a foundational platform for power users to bootstrap their own applications. AVBS abstracts the specifics of runtime interaction with OpenStudio, Modelica, and Spawn of EnergyPlus models behind a unified REST API. Additionally, AVBS provides resources for cloud deployment and scaling to 100s of parallel simulations, a growing library of modular Operational Technology (OT) integrations for emulation of real-world interfaces, and scripts to automate the population of communities of virtual buildings from URBANopt, ResStock and ComStock.

building automation↗
DOE OSTI2025

Root and Leaf Traits of Alfalfa Exhibit Distinct Responses to Soil Microbial Communities and Environmental Stresses

Ongoing climate change is negatively impacting crop productivity globally. Past research has highlighted that a diverse soil microbial community and variation in plant traits for resource acquisition can mitigate the negative impacts of climate change factors on crop productivity. This study investigates the effects of two major environmental stressors—drought and salinity stress, on plant productivity, biomass allocation, and root and leaf trait responses under distinct soil microbial diversities. Our results showed that salinity stress had stronger negative impacts on plant productivity than drought stress. Shoot biomass decreased by 30% and 32.5% under drought and salinity stress, respectively, whereas the root biomass decreased by 32% only under salinity stress. Soil microbial diversity did not affect plant productivity. Next, root traits were mainly impacted by drought and salinity stress, whereas leaf traits were impacted by both environmental stresses and soil microbial diversity. Specific root length and specific root area decreased under drought, and root tissue density was minimal under salinity stress. Root traits were not affected by soil microbial communities. In contrast, the leaf nitrogen content increased, whereas pheophytin content (a breakdown product of chlorophyll) decreased when plants were grown in diverse microbial communities under environmental stresses, especially drought. These results highlight the importance of soil microbial diversity in impacting plant traits in response to environmental stresses. We showed that the soil microbial diversity influences both aboveground and belowground plant traits, indicating the need for better management practices to conserve and promote soil microbial diversity.

59 BASIC BIOLOGICAL SCIENCES↗
DOE OSTI2024

Soil carbon change in intensive agriculture after 25 years of conservation management

Changes in soil organic carbon (SOC) and nitrogen (SON) are strongly affected by land management but few long-term comparative studies have surveyed changes throughout the whole soil profile. We quantified 25-year SOC and SON changes to 1 m in 10 replicate ecosystems at an Upper Midwest, USA site. We compared four annual cropping systems in maize (Zea mays)-soybean (Glycine max)-winter wheat (Triticum aestivum) rotations, each managed differently (Conventional, No-till, Reduced input, and Biologically based); in three managed perennial systems (hybrid Poplar (Populus × euramericana), Alfalfa (Medicago sativa), and Conifer (Pinus spp.); and in three successional systems (Early, Mid- and Late succession undergoing a gradual change in species composition and structure over time). Both Reduced input and Biologically based systems included winter cover crops. Neither SOC nor SON changed significantly in the Conventional or Late successional systems over 25 years. All other systems gained SOC and SON to different degrees. SOC accrual was fastest in the Early successional system (0.8 ± 0.1 Mg C ha –1 y –1 ) followed by Alfalfa and Conifer (avg. 0.7 ± 0.1 Mg C ha –1 y –1 ), Poplar, Reduced input, and Biologically based systems (avg. 0.4 ± 0.1 Mg C ha –1 y –1 ), and Mid-successional and No-till systems (0.3 and 0.2 Mg C ha –1 y –1 , respectively). Over the most recent 12 years, rates of SOC accrual slowed in all systems except Reduced input and Mid-successional. There was no evidence of SOC loss at depth in any system, including No-till. Rates of SON accrual ranged from 64.7 to 0.8 kg N ha –1 y –1 in the order Alfalfa ≥ Early successional > Reduced input and Biologically based ≥ Poplar > No-till and Conifer > Mid-successional systems. Pyrogenic C levels in the Conventional, Early, and Late successional systems were similar despite 17 years of annual burning in the Early successional system (~ 15 % of SOC to 50 cm, on average, and ~40 % of SOC from 50 to 100 cm). Results underscore the importance of cover crops, perennial crops, and no-till options for sequestering whole profile C in intensively managed croplands.

60 APPLIED LIFE SCIENCES↗
DOE OSTI2025

AmeriFlux FLUXNET-1F US-DFK Dairy Forage Research Center - Kernza

This is the AmeriFlux Management Project (AMP) created FLUXNET-1F version of the carbon flux data for the site US-DFK Dairy Forage Research Center - Kernza. This is the FLUXNET version of the carbon flux data for the site US-DFK Dairy Forage Research Center - Kernza produced by applying the standard ONEFlux (1F) software. Site Description - Crop field (10 acres) in annual and perennial rotation. Alfalfa (2015-2018), intermediate wheatgrass grown for Kernza grain and grass forage (planted September 2019-2023, terminated spring 2024), corn silage (2024), planted in winter wheat (late summer/fall 2024). Winter wheat (2025) followed by alfalfa (to be planted August 2025).

Duff, Alison [US Dairy Forage Research Center]↗
DOE OSTI2025

Quantifying soil organic matter stock distribution and origin following over a century of maize-based cropping in the former tallgrass prairie region of central USA

Tallgrass prairie conversion to maize-based agriculture in central North America has resulted in substantial loss of soil organic carbon (SOC) in less than two centuries. However, evaluations of how management practices may mitigate SOC losses are generally limited in soil depth and/or duration, missing long-term SOC stock outcomes that manifest over timescales of decades or longer. To address this, we sampled soils in year 145 of the Morrow Plots experiment to (i) evaluate effects of crop rotation and fertility management on SOC stocks and (ii) distinguish prairie- versus maize-derived SOC after continuous maize cropping since 1876 using stable carbon isotope ( 13 C) natural abundance. Soil organic carbon stock by equivalent soil mass (ESM) was + 30.7 Mg C ha −1 (+31.7 %) higher under maize-oat-alfalfa than continuous maize, but similar between maize-soybean and continuous maize. NPK fertilization and manuring did not influence SOC stocks by ESM. Response of SOC stocks at 15 cm depth intervals to NPK fertilization varied by depth and crop rotation, with lower SOC stocks at 30–45 cm under continuous maize and maize-soybean. Maize-derived C ranged 19.5–59.6 % of SOC stock across depths, indicating the majority of SOC was still derived from tallgrass prairie even after 145 years of continuous maize cropping. Our results confirm the potential of diversified crop rotation for minimizing SOC losses relative to tallgrass prairie at the supracentennial scale, and highlight the importance of relic prairie soil organic matter for future crop production in central North America.

crop rotation↗
DOE OSTI2024

AmeriFlux FLUXNET-1F US-UC1 LTAR UCB (Upper Chesapeake Bay) EC1

This is the AmeriFlux Management Project (AMP) created FLUXNET-1F version of the carbon flux data for the site US-UC1 LTAR UCB (Upper Chesapeake Bay) EC1. This is the FLUXNET version of the carbon flux data for the site US-UC1 LTAR UCB (Upper Chesapeake Bay) EC1 produced by applying the standard ONEFlux (1F) software. Site Description - Upper Chesapeake Bay farm is privately owned. The farming that took place was performed by the Farm Owner. The ground is rolling terrain, next to wooded areas, private resdiences and other large fields maintained by private land owners. At the time of this collection period, the site housed another Eddy Covariance System and a two Phenocams. Crop has been continuous corn with plans to rotate to alfalfa grass mixture.

Goslee, Sarah↗
DOE OSTI2024

AmeriFlux FLUXNET-1F US-UC2 LTAR UCB (Upper Chesapeake Bay) EC2

This is the AmeriFlux Management Project (AMP) created FLUXNET-1F version of the carbon flux data for the site US-UC2 LTAR UCB (Upper Chesapeake Bay) EC2. This is the FLUXNET version of the carbon flux data for the site US-UC2 LTAR UCB (Upper Chesapeake Bay) EC2 produced by applying the standard ONEFlux (1F) software. Site Description - Upper Chesapeake Bay farm is privately owned. The farming that took place was performed by the Farm Owner. The ground is rolling terrain, next to wooded areas, private resdiences and other large fields maintained by private land owners. At the time of this collection period, the site housed another Eddy Covariance System and a two Phenocams. Crop has been continuous corn with plans to rotate to alfalfa grass mixture.

Goslee, Sarah↗
DOE OSTI2025

AmeriFlux US-UTD UFLUX Dugout Ranch

This is the AmeriFlux version of the carbon flux data for the site US-UTD UFLUX Dugout Ranch. Site Description - The station is located along a fence line between two fields that are managed indentically. The land manager plants an alfalfa mix crop and uses wheel line irrigation.

Inkenbrandt, Paul [Utah Geological Survey]↗
DOE OSTI2025

AmeriFlux US-UTE UFLUX Escalante

This is the AmeriFlux version of the carbon flux data for the site US-UTE UFLUX Escalante. Site Description - This station is on the edge of an alfalfa field

Inkenbrandt, Paul [Utah Geological Survey]↗
DOE OSTI2025

AmeriFlux US-UTJ UFLUX Bluff

This is the AmeriFlux version of the carbon flux data for the site US-UTJ UFLUX Bluff. Site Description - This station is on the edge of an alfalfa field near the San Juan River

Inkenbrandt, Paul [Utah Geological Survey]↗
DOE OSTI2025

AmeriFlux US-UR8 Yellow Jacket - UCRB

This is the AmeriFlux version of the carbon flux data for the site US-UR8 Yellow Jacket - UCRB. Site Description - This site is located in Yellow Jacket, Colorado, on a moderate slope. The area is irrigated using a center pivot system, with alfalfa being the dominant vegetation. The region experiences a temperate climate, characterized by cool to mild temperatures throughout the year and substantial annual precipitation, creating favorable conditions for agricultural activities.

Neale, C. U. [Daugherty Water for Food Global Inst↗
DOE OSTI2025

AmeriFlux US-UR5 La Plata - UCRB

This is the AmeriFlux version of the carbon flux data for the site US-UR5 La Plata - UCRB. Site Description - This site is located in La Plata, New Mexico, in a flat terrain and part of a furrow irrigation field. The region has a semi-arid climate, with warm summers and mild winters. The area is primarily covered by alfalfa, which thrives in the irrigated environment.

Neale, C. U. [Daugherty Water for Food Institute/U↗
DOE OSTI2025

AmeriFlux US-UR6 Napi - UCRB

This is the AmeriFlux version of the carbon flux data for the site US-UR6 Napi - UCRB. Site Description - This site is located in Farmington, New Mexico, within a flat terrain and irrigated by a center pivot system. The region has a semi-arid climate, with warm summers and mild winters. The area is predominantly covered by alfalfa, which thrives in the irrigated conditions.

Neale, C. U. [Daugherty Water for Food Global Inst↗
DOE OSTI2025

AmeriFlux FLUXNET-1F US-UTD UFLUX Dugout Ranch

This is the AmeriFlux Management Project (AMP) created FLUXNET-1F version of the carbon flux data for the site US-UTD UFLUX Dugout Ranch. This is the FLUXNET version of the carbon flux data for the site US-UTD UFLUX Dugout Ranch produced by applying the standard ONEFlux (1F) software. Site Description - The station is located along a fence line between two fields that are managed indentically. The land manager plants an alfalfa mix crop and uses wheel line irrigation.

Inkenbrandt, Paul [Utah Geological Survey]↗
DOE OSTI2025

AmeriFlux FLUXNET-1F US-UTJ UFLUX Bluff

This is the AmeriFlux Management Project (AMP) created FLUXNET-1F version of the carbon flux data for the site US-UTJ UFLUX Bluff. This is the FLUXNET version of the carbon flux data for the site US-UTJ UFLUX Bluff produced by applying the standard ONEFlux (1F) software. Site Description - This station is on the edge of an alfalfa field near the San Juan River

Inkenbrandt, Paul [Utah Geological Survey]↗