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At least 19 records

Soil Carbon Change Due to Land Conversion to Grassland in a Semi-Arid Environment

A 5-year study evaluated the change in the quantity of soil total C (STC), soil organic C (SOC), and soil inorganic C (SIC) stored in the surface 60 cm of the soil profile on two adjacent blocks of land with a long-term history of cropping (CH) or undisturbed grassland (NH) on similar soil types between 1999 and 2004. The NH area was tilled and a grass-legume species mix was seeded into plots on both the NH and the CH areas. Selected plots of restored grass were established so they could be grazed (GG) by livestock while other plots were left ungrazed (UG). Original undisturbed (and ungrazed) grassland plots within the NH area were used as a control treatment. Initially, STC and SOC in CH were lower than NH when compared under the semi-arid environmental conditions found in southwestern North Dakota. Over the study period, the undisturbed grass control plots had increases in STC and SOC levels in the soil profile of 3.90 kg·m−2 and 3.34 kg·m−2, respectively. Restored grass on the NH area with grazing showed increases in STC and SOC values of 2.11 and 1.26 kg·m−2, respectively, while without grazing, profile STC and SOC had values of 3.80 and 3.28 kg·m−2, respectively. Restored grass on the CH area showed increases in profile STC and SOC values of 0.55 and 1.96 kg·m−2, respectively, for the grazed plots and 0.78 and 2.11 kg·m−2, respectively, when left ungrazed. Soil inorganic C, though present in the soils, did not significantly change during the study. The lower C accumulation in the CH plots may be due to a lag time in the establishment of mycorrhizal associations with the seeded species, the inoculums of which were already present in the NH soils. Changes in STC were likely due to changes in water relationships in the soil profile where management changes affected water infiltration and its movement causing leaching of SIC below the 60 cm depth evaluated. Soils under undisturbed grassland continue to accumulate carbon while soils of the disturbed grassland or cropped prior to re-establishing grass showed losses that occurred due to either accumulating C at a lower rate or perhaps to C loss during the initial establishment period (1–2 years).

Yellajosula, Gayatri↗

Controls on the 36 Cl/Cl input ratio of paleo-groundwater in arid environments: New evidence from 81 Kr/Kr data

In this study, measurements of the long-lived 81 Kr and 36 Cl radioisotopes in groundwater from the Negev Desert (Israel) were used to assess the 36 Cl/Cl input ratios and Cl – contents for paleorecharge into the Nubian Sandstone Aquifer (NSA). The reconstructed Cl – content of the recharge flux was on the order of 300–400 mg/L. An initial 36 Cl/Cl ratio of 50 × 10 –15 was assessed for the groundwater replenishment in the Negev Desert since the late Pleistocene, in agreement with the 36 Cl/Cl ratios in recent local rainwater. This is despite possible changes in the climatic conditions and the 36 Cl production rates in the atmosphere over this timeframe. This similarity in values is explained by the major role played by the erosion and weathering of near-surface materials in the desert environment that dominate the hydrochemistry of rains, floods, and the consequent groundwater recharge. Spatial variation in the reconstructed initial 36 Cl/Cl ratio is accounted for by the differences in the mineral aerosol sources for specific recharge areas of the NSA. Accordingly, regional variations in the 36 Cl/Cl input in groundwater reservoirs surrounding the Mediterranean Sea indicate various processes that govern the 36 Cl/Cl system. Finally, the results of this study highlight the great advantage of integrating 81 Kr age information in evaluating the initial 36 Cl/Cl and Cl – input, which is essential for the calibration of 36 Cl radioisotope as an available long-term dating tool for a given basin.

54 ENVIRONMENTAL SCIENCES↗

Convergent Hydraulic Redistribution and Groundwater Access Supported Facilitative Dependency Between Trees and Grasses in a Semi‐Arid Environment

Abstract Hydraulic redistribution is the transport of water from wet to dry soil layers, upward or downward, through plant roots. Often in savanna and woodland ecosystems, deep‐rooted trees, and shallow‐rooted grasses coexist. The degree to which these different species compete for or share soil‐water derived from precipitation or groundwater, as well as how these interactions are altered by hydraulic redistribution, is unknown. We use a multilayer canopy model and field observations to examine how the presence of deep, but tree‐root accessible, groundwater impacts seasonal patterns of hydraulic redistribution, and interaction between coexisting vegetation species in a semiarid riparian woodland (US‐CMW). Based on the simulation, trees absorb moisture at the water table (∼10 m depth) and release it in the shallow soil depth (0–3 m) during the dry pre‐monsoon season. We observed the occurrence of a new convergent hydraulic redistribution pattern during the monsoon season, where moisture is transported from both the near‐surface (0–0.5 m) and the water table to intermediate soil layers (1–5 m) through tree roots. We found that hydraulic redistribution demonstrates a growth facilitation effect at this site, supporting 49% of growing season tree transpiration and 14% of the grass transpiration. Compared to a similarly structured upland savanna without accessible groundwater, the riparian site shows an increased amount of hydraulically redistributed water and more facilitative water use between coexisting grasses and trees. These results shed light on the linkage between accessible groundwater and the role of hydraulic redistribution on the interaction between deep‐rooted and shallow‐rooted vegetation.

Lee, E.↗

Extreme heat vulnerability of manufactured housing in arid urban environments

This article explores the role of land cover in relation to housing type and tenure in shaping exposure to extreme urban heat, focusing on residents of mobile and manufactured housing (MH). We hypothesize that MH residents will experience greater exposure to extreme heat than those living in other housing types due to lower levels of proximate vegetation. This hypothesis is based on the unique property relations and tenure regimes that characterize MH, which may disincentivize investments in planting and maintaining trees and vegetation in arid environments. To test this hypothesis, we compare the amount of vegetation on properties across housing types, within-type tenure arrangements and between three urbanized areas in Arizona with different levels of exposure to extreme heat. To conduct this comparison, we combine multispectral land cover data for 1.7 million parcels with tax assessor data and building footprints to measure land cover at a high resolution. We find that MH units have significantly less vegetation than single-family residential properties, and that MH units in MH parks have less vegetation than those on individual lots. We conclude that municipalities should promote (e.g. through incentives or other policy interventions) the planting of more vegetation around MH units where there is a risk of extreme heat exposure. Future research can expand this analysis with a closer examination of how municipal ordinances and policies affect land cover by housing type and tenure.

heat↗

Characterization and microbial mitigation of fugitive methane emissions from oil and gas wells: Example from Indiana, USA

Hydrocarbon gas emissions from active, inactive, and improperly sealed or abandoned oil/gas wells significantly contribute to anthropogenically emitted greenhouse gases, predominantly in the form of methane (CH 4 ). We explored the extent of hydrocarbon gas emissions from 20 active, inactive, plugged and abandoned oil/gas wells in Indiana (USA), where it is estimated that there are more than 80,000 well sites throughout the state. After this initial survey, using a static flux tent, we quantified fugitive CH4 emissions from an active gas well to approximately 2 L h -1 . To evaluate the potential for microbial mitigation of hydrocarbon emissions to the atmosphere, we conducted laboratory microcosm experiments to quantify the CH 4 oxidizing potential of soils collected from sites with varying distances to the leaking gas well. Soils in close proximity to the well (0.5 m) efficiently consumed nearly all (97 %) of the added CH 4 , while only 14 % of added CH 4 was consumed by soils that were more distant from the well (20 m). These results suggest that fugitive CH 4 emissions enrich methanotrophic bacteria in soils immediately adjacent to the well. Consistent with this view, we found that prolonged exposure of soils to elevated concentrations of CH 4 enhanced the methanotrophic activity. Together, these findings prompted us to design a “methanotrophic soil mound” to assess the feasibility of mitigating point sources of CH 4 by harnessing the natural methanotrophic capacity of soil microbial communities. We found that a methanotrophic soil mound from a landfill could sustainably mitigate the CH 4 emission from the artificial source, providing a promising low-cost solution to ameliorate fugitive CH 4 emissions from abandoned oil and gas wells to the atmosphere. Finally, the effectiveness of microbe-based remediation is limited in cold climates and arid environments.

54 ENVIRONMENTAL SCIENCES↗

The role of environmental conditions on the carbonation of an alkali-activated cementitious waste form

Cast Stone is a cementitious waste form being considered for the solidification of low activity waste at the DOE Hanford Site. Under near-surface disposal conditions in an arid environment, Cast Stone is subject to drying and carbonation which may impact retention of waste constituents. Here this study investigates the effects of environmental CO 2 concentration and relative humidity (RH) on the carbonation of Cast Stone. The rate of carbonation front ingress and the extent of carbonation reaction were characterized for samples aged up to 48 weeks at three RH levels and two CO 2 concentrations. While the 68% RH environment allowed the greatest reaction extent, the 15% RH environment yielded the deepest carbonation front ingress. At 68% RH, there was a linear relationship between the ingress rate and CO 2 concentration. Carbonation reactions increased the drying rate of Cast Stone. Redistribution of sodium toward the wetting front was observed under drying and carbonation conditions.

36 MATERIALS SCIENCE↗

Evaluation of UAV-derived multimodal remote sensing data for biomass prediction and drought tolerance assessment in bioenergy sorghum

Screening for drought tolerance is critical to ensure high biomass production of bioenergy sorghum in arid or semi-arid environments. The bottleneck in drought tolerance selection is the challenge of accurately predicting biomass for a large number of genotypes. Although biomass prediction by low-altitude remote sensing has been widely investigated on various crops, the performance of the predictions are not consistent, especially when applied in a breeding context with hundreds of genotypes. In some cases, biomass prediction of a large group of genotypes benefited from multimodal remote sensing data; while in other cases, the benefits were not obvious. In this study, we evaluated the performance of single and multimodal data (thermal, RGB, and multispectral) derived from an unmanned aerial vehicle (UAV) for biomass prediction for drought tolerance assessments within a context of bioenergy sorghum breeding. The biomass of 360 sorghum genotypes grown under well-watered and water-stressed regimes was predicted with a series of UAV-derived canopy features, including canopy structure, spectral reflectance, and thermal radiation features. Biomass predictions using canopy features derived from the multimodal data showed comparable performance with the best results obtained with the single modal data with coefficients of determination (R 2 ) ranging from 0.40 to 0.53 under water-stressed environment and 0.11 to 0.35 under well-watered environment. The significance in biomass prediction was highest with multispectral followed by RGB and lowest with the thermal sensor. Finally, two well-recognized yield-based drought tolerance indices were calculated from ground truth biomass data and UAV predicted biomass, respectively. Results showed that the geometric mean productivity index outperformed the yield stability index in terms of the potential for reliable predictions by the remotely sensed data. Collectively, this study demonstrated a promising strategy for the use of different UAV-based imaging sensors to quantify yield-based drought tolerance.

09 BIOMASS FUELS↗

MOF Linker Extension Strategy for Enhanced Atmospheric Water Harvesting

A linker extension strategy for generating metal–organic frameworks (MOFs) with superior moisture-capturing properties is presented. Applying this design approach involving experiment and computation results in MOF-LA2-1 {[Al(OH)(PZVDC)], where PZVDC 2– is (E)-5-(2-carboxylatovinyl)-1H-pyrazole-3-carboxylate}, which exhibits an approximately 50% water capacity increase compared to the state-of-the-art water-harvesting material MOF-303. The power of this approach is the increase in pore volume while retaining the ability of the MOF to harvest water in arid environments under long-term uptake and release cycling, as well as affording a reduction in regeneration heat and temperature. Density functional theory calculations and Monte Carlo simulations give detailed insight pertaining to framework structure, water interactions within its pores, and the resulting water sorption isotherm.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗

Agrivoltaics as a climate-smart and resilient solution for midday depression in photosynthesis in dryland regions

Global projections of increased temperature and aridity have exacerbated concerns over our potential to achieve Sustainable Development Goals associated with food, energy, and water futures. We evaluated the implications of an agrivoltaics approach—combining agriculture and solar photovoltaics—on the microclimate growing conditions of crop species. While agrivoltaics is being explored globally for its potential to reduce soil evaporation rates and impact yield, empirical research on the mechanistic drivers of the potential for agrivoltaics is needed. Agrivoltaics mitigated the midday depression in photosynthesis experienced by crops grown in hot and arid environments, which led to reduced water stress, equal or greater daily carbon assimilation, and equal or greater yield across all crops. Our findings indicate agrivoltaics could be a climate-smart agricultural approach, and the diurnal resolution of our data points towards mechanisms for optimizing agrivoltaic designs to align with Sustainable Development Goals for food and energy production and water savings.

14 SOLAR ENERGY↗

Agrivoltaic system design tools for managing trade-offs between energy production, crop productivity and water consumption

Abstract Agrivoltaic systems that locate crop production and photovoltaic energy generation on the same land have the potential to aid the transition to renewable energy by reducing the competition between food, habitat, and energy needs for land while reducing irrigation requirements. Experimental efforts to date have not adequately developed an understanding of the interaction among local climate, array design and crop selection sufficient to manage trade-offs in system design. This study simulates the energy production, crop productivity and water consumption impacts of agrivoltaic array design choices in arid and semi-arid environments in the Southwestern region of the United States. Using the Penman–Monteith evapotranspiration model, we predict agrivoltaics can reduce crop water consumption by 30%–40% of the array coverage level, depending on local climate. A crop model simulating productivity based on both light level and temperature identifies afternoon shading provided by agrivoltaic arrays as potentially beneficial for shade tolerant plants in hot, dry settings. At the locations considered, several designs and crop combinations exceed land equivalence ratio values of 2, indicating a doubling of the output per acre for the land resource. These results highlight key design axes for agrivoltaic systems and point to a decision support tool for their development.

Environmental Sciences & Ecology↗

Genomic Analysis of Diverse Members of the Fungal Genus Monosporascus Reveals Novel Lineages, Unique Genome Content and a Potential Bacterial Associate

The genus Monosporascus represents an enigmatic group of fungi important in agriculture and widely distributed in natural arid ecosystems. Of the nine described species, two (M. cannonballus and M. eutypoides) are important pathogens on the roots of members of Cucurbitaceae in agricultural settings. The remaining seven species are capable of colonizing roots from a diverse host range without causing obvious disease symptoms. Recent molecular and culture studies have shown that members of the genus are nearly ubiquitous as root endophytes in arid environments of the Southwestern United States. Isolates have been obtained from apparently healthy roots of grasses, shrubs and herbaceous plants located in central New Mexico and other regions of the Southwest. Phylogenetic and genomic analyses reveal substantial diversity in these isolates. The New Mexico isolates include close relatives of M. cannonballus and M. ibericus, as well as isolates that represent previously unrecognized lineages. To explore evolutionary relationships within the genus and gain insights into potential ecological functions, we sequenced and assembled the genomes of three M. cannonballus isolates, one M. ibericus isolate, and six diverse New Mexico isolates. The assembled genomes were significantly larger than what is typical for the Sordariomycetes despite having predicted gene numbers similar to other members of the class. Differences in predicted genome content and organization were observed between endophytic and pathogenic lineages of Monosporascus. Several Monosporascus isolates appear to form associations with members of the bacterial genus Ralstonia (Burkholdariaceae).

59 BASIC BIOLOGICAL SCIENCES↗

AmeriFlux FLUXNET-1F US-ADR Amargosa Desert Research Site (ADRS)

This is the AmeriFlux Management Project (AMP) created FLUXNET-1F version of the carbon flux data for the site US-ADR Amargosa Desert Research Site (ADRS). This is the FLUXNET version of the carbon flux data for the site US-ADR Amargosa Desert Research Site (ADRS) produced by applying the standard ONEFlux (1F) software. Site Description - This tower is located at the Amargosa Desert Research Site (ADRS). The U.S. Geological Survey (USGS) began studies of unsaturated zone hydrology at ADRS in 1976. Over the years, USGS investigations at ADRS have provided long-term "benchmark" information about the hydraulic characteristics and soil-water movement for both natural-site conditions and simulated waste-site conditions in an arid environment. The ADRS is located in a creosote-bush community adjacent to disposal trenches for low-level radioactive waste.

Moreo, Michael [U.S. Geological Survey]↗

Fencing affects movement patterns of two large carnivores in Southern Africa

The erection of peripheral fences around protected areas has been shown to adversely affect the movement behavior, genetic connectivity and long-term viability of animal populations. However, fencing is also used extensively to reduce human encroachment, limit poaching activities, and mitigate human–wildlife conflicts. Limited information on the movement behavior of carnivores is available along the periphery of protected areas, particularly in semi-arid environments. To quantify the potential effects of the 824 km perimeter fence surrounding the Etosha National Park (Etosha, Namibia) on the movement and space use of carnivores, we collected GPS movement data from 36 lions (Panthera leo) and seven spotted hyenas (Crocuta crocuta) over a 14-year period. For these two apex carnivores, we measured the impact of the Etosha fence on their movements (step lengths and path straightness), calculated the likelihood of fence crossings, and evaluated the spatial–temporal characteristics of these crossings. Our results indicated that lions and hyenas moved faster (longer step lengths) when closer to the fence. Lions showed no variation in path straightness with respect to the fence but hyenas had straighter movement paths when closer to the fence. When moving within the vicinity of the fence, lions had a 9% likelihood of crossing the fence, while hyenas were much more likely (18%) to do so, which suggests that the Etosha fence was more permeable for hyenas than for lions. Fence crossings predominantly occurred at night for both species. Lions were more likely to cross the fence during the cold dry season (May–August) whereas hyenas crossed more often during the warm wet season (December–April). Female and adult lions had a higher probability of crossing the fence than males and subadults. These findings could be useful in the development of appropriate strategies to mitigate human–carnivore conflicts and thus promote the conservation of carnivores in multiple-use areas along the periphery of the park. Further research is needed to better elucidate the impact of perimeter fences on a broader suite of carnivores and other large mammals, including species-specific variation in the response to fences, as well as the economic benefits of fencing provided to local communities.

59 BASIC BIOLOGICAL SCIENCES↗

Engineering crassulacean acid metabolism (CAM) pathways in plants

Disclosed herein are method of altering CAM pathways in plants. In some examples, a disclosed method includes overexpressing one or more genes encoding one or more enzymes that carry out the basic biochemical sequence of nocturnal CO2 fixation (carboxylation) into C4 acids (malate), store C4 acids in the vacuole of the plant, and/or then decarboxylate and refix the released CO2 by C3 photosynthesis during the subsequent day in a plant cell, thereby altering CAM in the plant cell. Also disclosed herein are isolated polynucleotide sequences, transformation vectors, transgenic plant cells, plant part, and plants. The disclosed methods and compositions can be used to improve the water-use efficiency and drought tolerance and durability of plants, such as in plants in arid environments, and also enhance the ability of plants to perform.

Cushman, John C.↗

Resource Characterization to Estimate Potential for Electricity Co-Production at Blackburn Oil Field, Nevada

The U.S. Department of Energy estimates that an annual average of 25 billion barrels of hot water are produced from oil and gas wells within the United States. The thermal energy available in the co-produced water stream is usually discarded, as the produced waters are considered an inconvenience by the operators and are disposed of using injection wells. However, utilizing organic Rankine cycle (ORC) generators, a vast amount of thermal energy can be captured and converted into electricity (albeit at relatively low efficiency due to the low temperatures). The National Renewable Energy Laboratory (NREL), in collaboration with Transitional Energy and Grant Canyon Oil & Gas, evaluated the feasibility of geothermal co-production of electricity by utilizing existing oil wells in Blackburn oil field in Nevada. The once prolific Blackburn oil field is located in Pine Valley, approximately 45 miles east-southeast of Elko, Nevada. Currently, the wells targeting the highly fractured Devonian Nevada dolomite reservoir are operating at a water cut ratio of more than 99%, with individual fluid (oil and water) production rates reaching 7.4 L/s (4,021 BBL/day). Analysis of publicly available data showed that the combination of the suitable wells' maximum historical production rates reached 22.90 L/s. The production from these wells occurs naturally and the wells are choked (and even shut down) by the operator to mitigate excessive water production, indicating a strong reservoir recharge and future opportunity to increase the water production for geothermal electricity generation. The main goal of this study was to evaluate the productivity of the existing wells, the performance of the reservoir, the surface network, and the operational constraints in order to achieve 1 MWe of electricity production from the field's water production. Utilizing the GEOPHIRES tool, we have determined that a twofold to threefold increase in the total fluid production, compared to the historical production under artificial restraint (choke), is required to reach a 1-MWe net target output for a low-temperature ORC system with air-cooled condensers. Lower flow rates would be required when utilizing water-based condensers instead of air-cooled condensers. However, that would require a constant supply of cold water, which may be challenging given the arid environment of the project site.

Blackburn↗

California Trees Seasonally Use Augmented Water Sources: Water Isotope Tracking in a Groundwater‐Dependent Ecosystem

Sustainable groundwater management must account for the needs of groundwater dependent ecosystems. To understand the relationship of ecosystems and seasonal water use, we studied the stable isotope composition (δ 18 O and δ 2 H) of water in streamside trees in a semi-arid streamside environment (Livermore, California, USA). We sampled seven trees at two sites every other month from April 2024 through April 2025 for tree xylem stable water isotope signatures. These data were compared to potential source waters: precipitation, imported surface water, soil water and regional groundwaters. Large daily precipitation events were found to be isotopically similar to regional groundwater and were thus treated as one water source. A Bayesian mixing model using stable water isotopes was used to determine the ratios of these three potential source waters (small daily precipitation events, groundwater/large daily precipitation events and imported water) present in tree xylem water. On average, tree water sources include 32% imported water (SD = 9%), 35% small daily precipitation events (SD = 10%) and 33% groundwater (SD = 3%), with significant seasonal variation (t summer-winter = 30.8, p < 0.01), particularly drawing more imported water (more than 55%) in the summer. While small daily precipitation events contribute only 10% of the total precipitation in our dataset, it represents a third of water used by trees. In addition, while these ecosystems are designated as groundwater dependent ecosystems, the trees use approximately one third imported water and even more during dry summer months. This approach provides water managers with a practical tool for quantifying ecosystem water needs, supporting data-driven decisions and regulatory compliance. While California's Sustainable Groundwater Management Act emphasizes supporting groundwater dependent ecosystems, it allows flexibility in demonstrating benefits. In conclusion, our methodology provides a way to document that management actions (such as managed aquifer recharge with imported water) deliver measurable co-benefits to GDEs.

Geosciences↗

Desert truffle genomes reveal their reproductive modes and new insights into plant–fungal interaction and ectendomycorrhizal lifestyle

Desert truffles are edible hypogeous fungi forming ectendomycorrhizal symbiosis with plants of Cistaceae family. Knowledge about the reproductive modes of these fungi and the molecular mechanisms driving the ectendomycorrhizal interaction is lacking. Genomes of the highly appreciated edible desert truffles Terfezia claveryi Chatin and Tirmania nivea Trappe have been sequenced and compared with other Pezizomycetes. Transcriptomes of T. claveryi × Helianthemum almeriense mycorrhiza from well-watered and drought-stressed plants, when intracellular colonizations is promoted, were investigated. We have identified the fungal genes related to sexual reproduction in desert truffles and desert-truffles-specific genomic and secretomic features with respect to other Pezizomycetes, such as the expansion of a large set of gene families with unknown Pfam domains and a number of species or desert-truffle-specific small secreted proteins differentially regulated in symbiosis. A core set of plant genes, including carbohydrate, lipid-metabolism, and defence-related genes, differentially expressed in mycorrhiza under both conditions was found. Our results highlight the singularities of desert truffles with respect to other mycorrhizal fungi while providing a first glimpse on plant and fungal determinants involved in ecto to endo symbiotic switch that occurs in desert truffle under dry conditions.

59 BASIC BIOLOGICAL SCIENCES↗

Cliff Retreat Rates Associated with a Low-Level Radioactive Waste Disposal Facility in Los Alamos, New Mexico, USA

We present an analysis and interpretation of potential cliff stability at a low-level waste disposal facility at Los Alamos National Laboratory, New Mexico, using cliff morphologic and fracture characteristics coupled with carbon-14 surface exposure dating. Our study is important as it directly bears on the licensing criteria for low-level radioactive waste sites. We find that future characteristic cliff failures will likely not breach disposal pits and shafts over the 1000-year minimum regulatory period. Further, we find, using a multivariate regression model, that slope angle and cliff face aspect are sub-equal in importance to predict regions of high risk of failure when combined with surface exposure ages and assuming that old exposure ages are most indicative of stability (instability) and therefore can aid decision making in final design implementation.

12 MANAGEMENT OF RADIOACTIVE AND NON-RADIOACTIVE W↗