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At least 19 records

A novel transfer learning framework for sorghum biomass prediction using UAV-based remote sensing data and genetic markers

Yield for biofuel crops is measured in terms of biomass, so measurements throughout the growing season are crucial in breeding programs, yet traditionally time- and labor-consuming since they involve destructive sampling. Modern remote sensing platforms, such as unmanned aerial vehicles (UAVs), can carry multiple sensors and collect numerous phenotypic traits with efficient, non-invasive field surveys. However, modeling the complex relationships between the observed phenotypic traits and biomass remains a challenging task, as the ground reference data are very limited for each genotype in the breeding experiment. In this study, a Long Short-Term Memory (LSTM) based Recurrent Neural Network (RNN) model is proposed for sorghum biomass prediction. The architecture is designed to exploit the time series remote sensing and weather data, as well as static genotypic information. As a large number of features have been derived from the remote sensing data, feature importance analysis is conducted to identify and remove redundant features. A strategy to extract representative information from high-dimensional genetic markers is proposed. To enhance generalization and minimize the need for ground reference data, transfer learning strategies are proposed for selecting the most informative training samples from the target domain. Consequently, a pre-trained model can be refined with limited training samples. Field experiments were conducted over a sorghum breeding trial planted in multiple years with more than 600 testcross hybrids. The results show that the proposed LSTM-based RNN model can achieve high accuracies for single year prediction. Further, with the proposed transfer learning strategies, a pre-trained model can be refined with limited training samples from the target domain and predict biomass with an accuracy comparable to that from a trained-from-scratch model for both multiple experiments within a given year and across multiple years.

36 MATERIALS SCIENCE↗

Predicting Biomass Yields of Advanced Switchgrass Cultivars for Bioenergy and Ecosystem Services Using Machine Learning

The production of advanced perennial bioenergy crops within marginal areas of the agricultural landscape is gaining interest due to its potential to sustainably produce feedstocks for biofuels and bioproducts while also improving the sustainability and resilience of commodity crop production. However, predicting the biomass yields of this production system is challenging because marginal areas are often relatively small and spread around agricultural fields and are typically associated with various abiotic conditions that limit crop production. Machine learning (ML) offers a viable solution as a biomass yield prediction tool because it is suited to predicting relationships with complex functional associations. The objectives of this study were to (1) evaluate the accuracy of commonly applied ML algorithms in agricultural applications for predicting the biomass yields of advanced switchgrass cultivars for bioenergy and ecosystem services and (2) determine the most important biomass yield predictors. Datasets on biomass yield, weather, land marginality, soil properties, and agronomic management were generated from three field study sites in two U.S. Midwest states (Illinois and Iowa) over three growing seasons. The ML algorithms evaluated in the study included random forests (RFs), gradient boosting machines (GBMs), artificial neural networks (ANNs), K-neighbors regressor (KNR), AdaBoost regressor (ABR), and partial least squares regression (PLSR). Coefficient of determination (R 2 ) and mean absolute error (MAE) were used to evaluate the predictive accuracy of the tested algorithms. Results showed that the ensemble methods, RF (R 2 = 0.86, MAE = 0.62 Mg/ha), GBM (R 2 = 0.88, MAE = 0.57 Mg/ha), and GBM (R 2 = 0.78, MAE = 0.66 Mg/ha), were the most accurate in predicting biomass yields of the Independence, Liberty, and Shawnee switchgrass cultivars, respectively. This is in agreement with similar studies that apply ML to multi-feature problems where traditional statistical methods are less applicable and datasets used were considered to be relatively small for ANNs. Consistent with previous studies on switchgrass, the most important predictors of biomass yield included average annual temperature, average growing season temperature, sum of the growing season precipitation, field slope, and elevation. This study helps pave the way for applying ML as a management tool for alternative bioenergy landscapes where understanding agronomic and environmental performance of a multifunctional cropping system seasonally and interannually at the sub-field scale is critical.

09 BIOMASS FUELS↗

Nitrogen fertilization effects on aged Miscanthus × giganteus stands: Exploring biomass yield, yield components, and biomass prediction using in–season morphological traits

For sustainable biomass production of Miscanthus × giganteus (hereafter miscanthus), understanding the impact of stand age and nitrogen (N) fertilization on biomass yield is crucial. This study investigated the effects of varying N fertilization rates (0, 56, 112, and 168 kg N ha –1 ) on yield components (tiller height, density, and weight) and their correlations with end-of-season biomass yield in miscanthus. We also explored end-of-season biomass yield prediction using in-season traits (canopy height, leaf area index, and leaf chlorophyll content [LCC]). The study was conducted at two sites in Illinois: a previously unfertilized 10-year-old miscanthus research stand at Urbana and a 16-year-old commercial stand at Pesotum with a history of annual 56N application. Results from 2018 to 2021 in Urbana and 2020 to 2021 in Pesotum showed increased biomass yields with N fertilization, varying by rate, year, and location. Biomass yield in Pesotum peaked at 56N, while in Urbana, it increased significantly at 112 kg N ha –1 . Biomass yield was strongly correlated with tiller height and weight measured at Urbana across N rates. Morphological traits measured every 2–3 weeks during the 2020 and 2021 growing seasons showed that canopy height was the strongest single predictor of miscanthus biomass yield, followed by LCC. Mid-August to September measurements of these traits were the best predictors of biomass yield. Multiple regressions involving the canopy height and LCC further improved yield predictions. We conclude that while N enhances biomass yields of aging miscanthus, the optimum rate depends on the site, environmental conditions, and management history.

59 BASIC BIOLOGICAL SCIENCES↗

Using leaf and stomatal traits to predict biomass production and water use efficiency in Populus

Climate change is reshaping ecosystems, driving plants to adapt through leaf-trait plasticity that reflects strategies for growth and water use. Predicting biomass production and intrinsic water use efficiency (iWUE) remains challenging because of genetic, taxonomic, and environmental variability. Here, we used eastern cottonwood and Populus hybrids as a model system to test whether easily measurable leaf traits can serve as reliable predictors of performance, and whether adding stomatal and biochemical traits improves predictive power. Across two field sites in Mississippi, leaf mass per area (LMA), biomass production, iWUE, leaf area, and foliar nitrogen ( N %) differed significantly among taxa and sites, while other traits were conserved. Factorial analysis of mixed data (FAMD) revealed distinct clustering of taxa and sites, indicating coordinated variation among leaf and stomatal traits. Pairwise correlations highlighted fundamental trade-offs, with biomass positively related to LMA and petiole length but negatively associated with iWUE, N %, and carbon isotopic ratios (δ 13 C). Leaf temperature and leaf angle varied among taxa and were significantly correlated with LMA and petiole length, suggesting mechanisms of heat dissipation and leaf movability that link simple traits to gas exchange and productivity. Weighted multiple linear regression models explained 80%–91% of variation in biomass production and iWUE. Models using only LMA, petiole length, and stomatal metrics performed nearly as well as those incorporating N %, and δ 13 C, with complex traits adding approximately 10% explanatory power. These results demonstrate that simple morphological traits capture integrated functional trade-offs, while complex traits refine predictions. This tiered approach provides an efficient framework for selecting high-yielding, water-efficient genotypes of Populus and other hardwood species, offering practical pathways to enhance carbon uptake and iWUE under climate change.

biomass production↗

Challenges to Aboveground Biomass Prediction from Waveform Lidar

Accurate accounting of aboveground biomass density (AGBD) is crucial for carbon cycle, biodiversity, and climate change science. The Global Ecosystem Dynamics Investigation (GEDI), which maps global AGBD from waveform lidar, is the first of a new generation of Earth observation missions designed to improve carbon accounting. This paper explores the possibility that lidar waveforms may not be unique to AGBD—that forest stands with different AGBD may produce highly similar waveforms—and we hypothesize that non-uniqueness may contribute to the large uncertainties in AGBD predictions. Our analysis integrates simulated GEDI waveforms from 428 in situ stem maps with output from an individual-based forest gap model, which we use to generate a database of potential forest stands and simulate GEDI waveforms from those stands. We use this database to predict the AGBD of the 428 in situ stem maps via two different methods: a linear regression from waveform metrics, and a waveform-matching approach that accounts for waveform-AGBD non-uniqueness. We find that some in situ waveforms are more unique to AGBD than others, which notably impacts AGBD prediction uncertainty (7–411 Mg ha−1, average of 167 Mg ha−1). We also find that forest structure complexity may influence the non-uniqueness effect; stands with low structural complexity are more unique to AGBD than more mature stands with multiple cohorts and canopy layers. These findings suggest that the non-uniqueness phenomena may be introduced by the measuring characteristics of waveform lidar in combination with how forest structure manifests at small scales, and we discuss how this complexity may complicate uncertainty estimation in AGBD prediction. This analysis suggests a limit to the accuracy and precision of AGBD predictions from lidar waveforms seen in empirical studies, and underscores the need for further exploration of the relationships between lidar remote sensing measurements, forest structure, and AGBD.

GEDI↗

Predicting biomass comminution: Physical experiment, population balance model, and deep learning

An extended population balance model (PBM) and a deep learning-based enhanced deep neural operator (DNO+) model are introduced for predicting particle size distribution (PSD) of comminuted biomass through a large knife mill. Experimental tests using corn stalks with varied moisture contents, mill blade speeds, and discharge screen sizes are conducted to support model development. A novel mechanism in the extended PBM allows for including additional input parameters such as moisture content, which is not possible in the original PBM. The DNO+ model can include influencing factors of different data types such as moisture content and discharge screen size, which significantly extends the engineering applicability of the standard DNO model that only admits feed PSD and outcome PSD. Test results show that both models are remarkably accurate in the calibration or training parameter space and can be used as surrogate models to provide effective guidance for biomass preprocessing design.

09 BIOMASS FUELS↗

Longitudinal genome-wide association study reveals early QTL that predict biomass accumulation under cold stress in sorghum

Sorghum bicolor is a promising cellulosic feedstock crop for bioenergy due to its high biomass yields. However, early growth phases of sorghum are sensitive to cold stress, limiting its planting in temperate environments. Cold adaptability is crucial for cultivating bioenergy and grain sorghum at higher latitudes and elevations, or for extending the growing season. Identifying genes and alleles that enhance biomass accumulation under early cold stress can lead to improved sorghum varieties through breeding or genetic engineering. We conducted image-based phenotyping on 369 accessions from the sorghum Bioenergy Association Panel (BAP) in a controlled environment with early cold treatment. The BAP includes diverse accessions with dense genotyping and varied racial, geographical, and phenotypic backgrounds. Daily, non-destructive imaging allowed temporal analysis of growth-related traits and water use efficiency (WUE). A genome-wide association study (GWAS) was performed to identify genomic intervals and genes associated with cold stress response. The GWAS identified transient quantitative trait loci (QTL) strongly associated with growth-related traits, enabling an exploration of the genetic basis of cold stress response at different developmental stages. This analysis of daily growth traits, rather than endpoint traits, revealed early transient QTL predictive of final phenotypes. The study identified both known and novel candidate genes associated with growth-related traits and temporal responses to cold stress. The identified QTL and candidate genes contribute to understanding the genetic mechanisms underlying sorghum's response to cold stress. These findings can inform breeding and genetic engineering strategies to develop sorghum varieties with improved biomass yields and resilience to cold, facilitating earlier planting, extended growing seasons, and cultivation at higher latitudes and elevations.

59 BASIC BIOLOGICAL SCIENCES↗

Predicted aboveground biomass of Typha angustifolia in an upland brackish tidal marsh, PIE LTER, Byfield, MA (2022-2024)

This dataset contains predicted monthly aboveground Typha angustifolia biomass per sample and per square meter in a brackish tidal marsh site dominated by Typha angustifolia near the Parker River in the upper estuary of the Plum Island Sound, Massachusetts (MA) during the growing seasons (May-September) of 2022, 2023, and 2024. This site is also located within the Plum Island Ecosystems Long Term Ecological Research Station (PIE LTER). Allometric equations were developed from dry weight data and associated maximum heights collected in 2022 and 2023. The goal of this study was to investigate the difference in aboveground biomass between the site’s marsh interior (MI) and the creek bank (CB). Metadata files (Typha_biomass_predictions_dd.csv and Typha_biomass_predictions_flmd.csv) contain detailed information on variable definitions, calculations, sampling methods, and the location of the site.

DATE↗

Estimating Switchgrass Biomass Yield and Lignocellulose Composition from UAV-Based Indices

Innovative methods for estimating commercial-scale switchgrass yields and feedstock quality are essential to optimize harvest logistics and biorefinery efficiency for sustainable aviation fuel production. This study utilized vegetation indices (VIs) derived from multispectral images to predict biomass yield and lignocellulose concentrations of advanced bioenergy-type switchgrass cultivars (“Liberty” and “Independence”) under two N rates (28 and 56 kg N ha –1 ). Field-scale plots were arranged in a randomized complete block design (RCBD) and replicated three times at Urbana, IL. Multispectral images captured during the 2021–2023 growing seasons were used to extract VIs. The results show that linear and exponential models outperformed partial least square and random forest models, with mid-August imagery providing the best predictions for biomass, cellulose, and hemicellulose. The green normalized difference vegetation index (GNDVI) was the best univariate predictor for biomass yield (R 2 = 0.86), while a multivariate combination of the GNDVI and normalized difference red-edge index (NDRE) enhanced prediction accuracy (R 2 = 0.88). Cellulose was best predicted using the NDRE (R 2 = 0.53), whereas hemicellulose prediction was most effective with a multivariate model combining the GNDVI, NDRE, NDVI, and green ratio vegetation index (GRVI) (R 2 = 0.44). These findings demonstrate the potential of UAV-based VIs for the in-season estimation of biomass yield and cellulose concentration.

09 BIOMASS FUELS↗

Colorado Eastern Plains Agriculture: Rangeland Monitoring to Inform Grazing Management in Eastern Colorado

Adaptive management on cattle ranches requires rangeland managers to decide the location and duration of the cattle grazing activity across different pastures. Biodiversity, forage availability, and cattle health are all affected by rangeland management. Virtual fencing is a tool that rangeland managers can use to potentially increase biodiversity and improve ranching operations. NASA DEVELOP and Colorado State University (CSU) collaborated with the Nature Conservancy (TNC), and Red Top Ranch to demonstrate the efficacy of virtual fencing. We sought to identify annual and monthly biomass patterns on the ranch through the creation of monthly max biomass productivity maps. We utilized a dataset from the Agricultural Research Service (ARS) to calculate biomass on the ranch. To validate our remotely-sensed results, we compared model-predicted biomass values to field-collected biomass clipping data and an additional biomass dataset from the Rangeland Analysis Platform (RAP). We used satellite imagery from Landsat 5 Thematic Mapper (TM), Landsat 7 Enhanced Thematic Mapper Plus (ETM+), Landsat 8 Operational Land Imager (OLI), Landsat 9 –OLI-2, and Sentinel-2 MultiSpectral Instrument (MSI) for 2021 and 2022. We found that there was heterogeneity in biomass across the ranch, with higher biomass on the western side. The highest peak of biomass was in the summer months, with a smaller peak in mid-September. The ARS biomass dataset had a significant relationship with RAP for 2021. ARS biomass did not have a significant relationship with the biomass field data collected in 2022. The results of our study are aimed to support rotation management, ranch production, biodiversity, and conservation management.

cattle↗

Next generation Arctic vegetation maps: Aboveground plant biomass and woody dominance mapped at 30 m resolution across the tundra biome

The Arctic is warming faster than anywhere else on Earth, placing tundra ecosystems at the forefront of global climate change. Plant biomass is a fundamental ecosystem attribute that is sensitive to changes in climate, closely tied to ecological function, and crucial for constraining ecosystem carbon dynamics. However, the amount, functional composition, and distribution of plant biomass are only coarsely quantified across the Arctic. Therefore, we developed the first moderate resolution (30 m) maps of live aboveground plant biomass (g m −2 ) and woody plant dominance (%) for the Arctic tundra biome, including the mountainous Oro Arctic. We modeled biomass for the year 2020 using a new synthesis dataset of field biomass harvest measurements, Landsat satellite seasonal synthetic composites, ancillary geospatial data, and machine learning models. Additionally, we quantified pixel-wise uncertainty in biomass predictions using Monte Carlo simulations and validated the models using a robust, spatially blocked and nested cross-validation procedure. Observed plant and woody plant biomass values ranged from 0 to ∼6000 g m −2 (mean ≈ 350 g m −2 ), while predicted values ranged from 0 to ∼4000 g m −2 (mean ≈ 275 g m −2 ), resulting in model validation root-mean-squared-error (RMSE) ≈ 400 g m −2 and R 2 ≈ 0.6. Our maps not only capture large-scale patterns of plant biomass and woody plant dominance across the Arctic that are linked to climatic variation (e.g., thawing degree days), but also illustrate how fine-scale patterns are shaped by local surface hydrology, topography, and past disturbance. By providing data on plant biomass across Arctic tundra ecosystems at the highest resolution to date, our maps can significantly advance research and inform decision-making on topics ranging from Arctic vegetation monitoring and wildlife conservation to carbon accounting and land surface modeling.

Climate change↗

Comprehensive Comparison of Airborne and Spaceborne SAR and LiDAR Estimates of Forest Structure in the Tallest Mangrove Forest on Earth

A recent suite of new global-scale satellite sensors and regional-scale airborne campaigns are providing a wealth of remote sensing data capable of dramatically advancing our current understanding of the spatial distribution of forest structure and carbon stocks. However, a baseline for forest stature and biomass estimates has yet to be established for the wide array of available remote sensing products. At present, it remains unclear how the estimates from these sensors compare to one another in terrestrial forests, with a clear dearth of studies in high carbon density mangrove ecosystems. In the tallest mangrove forest on Earth (Pongara National Park, Gabon), we leverage the data collected during the AfriSAR campaign to evaluate 17 state-of-the-art sensor data products across the full range of height and biomass known to exist globally in mangrove forest ecosystems, providing a much-needed baseline for sensor performance. Our major findings are: (Houghton, Hall, Goetz) height estimates are not consistent across products, with opposing trends in relative and absolute errors, highlighting the need for an adaptive approach to constraining height estimates (Panet al., 2011); radar height estimates had the lowest calibration error and bias, with further improvements using LiDAR fusion (Bonan, 2008); biomass variability and uncertainty strongly depends on forest stature, with variation across products increasing with canopy height, while relative biomass variation was highest in low-stature stands (Le Quéréet al., 2017); a remote sensing product's sensitivity to variations in canopy structure is more important than the absolute accuracy of height estimates (Mitchardet al., 2014); locally-calibrated area-wide totals are more representative than generalized global biomass models for high-precision biomass estimates. The findings presented here provide critical baseline expectations for height and biomass predictions across the full range of mangrove forest stature, which can be directly applied to current (TanDEM-X, GEDI, ICESat-2) and future (NISAR, BIOMASS) global-scale forest monitoring missions.

Atticus E.L. Stovall↗

Autonomous monitoring of algal biomass: Success stories and lessons learned from long-term field deployment

Autonomous, high-frequency monitoring of outdoor algal ponds is needed to quantify biomass productivity and detect culture decline in environments prone to contamination, grazers, and variable operating conditions. We report successes and lessons learned in translating a laboratory spectroradiometric monitoring approach to a multi-year autonomous field deployment at the Arizona Center for Algae Technology and Innovation (AzCATI). The system measures spectrally resolved pond reflectance by ratioing upwelling radiance from each raceway to simultaneous downwelling sky irradiance using fiber-coupled spectrometers. A physics-based reflectance model (ASHARP) is fit to each spectrum pair to estimate optical parameters, including a biomass-proxy coefficient (C a ) which enables near-real-time tracking of biomass accumulation and culture state at 2–5 min intervals. From May 2022 through September 2025 the platform operated continuously while scaling from two to six raceway ponds. Several strains of algae were monitored successfully, including the high productivity Tetraselmis striata and Picochlorum celeri. Transitioning data acquisition from a Windows laptop to a Raspberry Pi improved uptime from 57% (2022) to ~89% (2024–2025) and enabled routine real-time analysis. Further, we converted relative biomass estimates to absolute ash-free dry weight (AFDW) using experimentally-derived calibrations, providing field-relevant biomass predictions with conservative confidence bounds. These results demonstrate the feasibility of long-term, autonomous optical monitoring for well-mixed open-raceway algal cultivation and provide practical guidance for reliable field operation and scaling.

Katinas, Christopher Michael [Sandia National Labo↗

RhizoNet: semantic segmentation of plant roots using CNN (RhizoNet) v0.0.1

RhizoNet is designed for the semantic segmentation of plant root scans, utilizing a sophisticated deep learning network known as Residual Unet. It specializes in processing color images of plants cultivated in a hydroponic EcoFAB system, captured using an Epson scanner. The core of the algorithm is based on Residual U-nets, which significantly improve prediction accuracy. This is achieved through the implementation of a convex hull operation, which effectively delineates the primary root component by leveraging spatio-temporal image data. This enables researchers to accurately assess predicted biomass and monitor plant growth over time.

Ushizima, Daniela↗

Scaling Spectroradiometric Monitoring to an Algal Factory Farm

This report briefly summarizes the spectroradiometric approach adapted and deployed as part of Task 4.2 within the BETO-funded D evelopment of I ntegrated S creening, C ultivar O ptimization, and V erification R esearch (DISCOVR) project, specifically task 4, “Increase crop protection and culture stability.” The goals of this subtask were to monitor ponds cultivating a variety of DISCOVR strains at the Arizona Center for Algae Technology and Innovation (AzCATI) with spectroradiometric technology to: • Predict biomass concentrations with high temporal frequency. • Optimize automated analysis methods that enabled feedback for pond automation strategies • Identify pond crash indicators from primary (pest/predator) and indirect (change in algal host) spectroscopic signals with potential for early treatment/intervention • Provide recommendations for technology deployment at scale. This report fulfills the deliverable requirement for the final bullet “provide recommendations for technology deployment at scale”.

09 BIOMASS FUELS↗

Applying double cropping and interactive irrigation in the North China Plain using WRF4.5

Abstract. Irrigated cultivation exerts a significant influence on the local climate and the hydrological cycle. The North China Plain (NCP) is known for its intricate agricultural system, marked by expansive cropland, high productivity, compact rotation, a semi-arid climate, and intensive irrigation practices. As a result, there has been considerable attention on the potential impact of this intensive irrigated agriculture on the local climate. However, studying the irrigation impact in this region has been challenging due to the lack of an accurate simulation of crop phenology and irrigation practices within the climate model. By incorporating double cropping with interactive irrigation, our study extends the capabilities of the Weather Research Forecast (WRF) model, which has previously demonstrated commendable performance in simulating single-cropping scenarios. This allows for two-way feedback between irrigated crops and climate, further enabling the inclusion of irrigation feedback from both ground and vegetation perspectives. The improved crop modeling system shows significant enhancement in capturing vegetation and irrigation patterns, which is evidenced by its ability to identify crop stages, estimate field biomass, predict crop yield, and project monthly leaf area index. The improved simulation of large-scale irrigated crops in the NCP can further enhance our understanding of the intricate relationship between agricultural development and climate change.

54 ENVIRONMENTAL SCIENCES↗