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At least 19 records

SPRUCE S1 Bog and Bog Lake Fen Moss Decomposition and Litter Chemistry Data, 2014-2019

This dataset includes measurements of mass loss, decay rate, and litter chemistry of mosses decomposing in the S1 Bog and Bog Lake Fen located in the USDA Forest Service Marcell Experimental Forest (MEF) in northern Minnesota, 40 km north of Grand Rapids. A litterbag experiment was used to estimate decay rates of 3 different moss types (Sphagnum magellanicum, Sphagnum angustifolium/fallax, and Polytrichum sp.) from mass loss measurements after one and five years in hummock and hollow microtopographies in the bog and lawn microtopography in the poor fen. Carbon, nitrogen, and phosphorous contents of the mosses were measured initially, after 1 year, and after 5 years of decay. Sample collection and analyses started in 2014 and was completed in 2019 (2014-06-05 to 2019-06-05). These data are from outside the Spruce and Peatland Responses Under Changing Environments (SPRUCE) experimental plots. Decomposition data (rates and chemistry changes) can be used to assess ecosystem carbon and nutrient cycles. These data were used in analyses reported in Shelley et al (2022). This dataset contains two data files in comma-separate values (*.csv) format. Additional metadata are provided: two data dictionaries and a file-level metadata file in comma-separate values (.csv) format and a user guide in PDF (*.pdf) format.

54 ENVIRONMENTAL SCIENCES↗

Variation in peatland porewater chemistry over time and space along a bog to fen gradient

Porewater chemistry is an integrative measure of the physical, chemical, and biological processes occurring within peatland ecosystems, and therefore some chemistry measures (e.g., pH, calcium concentrations) have been used to classify bog vs fen peatlands. However, porewater sampling is often limited in spatial and temporal resolution, highlighting the need for a more comprehensive analysis of spatiotemporal variation in porewater chemistry. We examined depth profiles of porewater chemistry in four nearby peatlands that fall along a bog to rich fen gradient in northcentral Minnesota, USA. Porewater was sampled ~monthly during one ice-free season from three replicate piezometer nests per peatland to quantify temporal and spatial variability of those depth profiles. Porewater depth profiles of pH, calcium and total organic carbon concentrations, and δ 18 O-H 2 O varied along the bog to fen gradient, but total nitrogen and total phosphorus concentrations did not. Porewater chemistry was similar in the bogs and poor fen which were all quite different from the rich fen. In contrast, temporal and spatial variation in porewater chemistry, quantified using coefficients of variation, did not differ between bogs and fens despite the hypothesis that variation in porewater chemistry would be lower in fens than bogs due to the perennial throughput of large volumes of discharging groundwater in the rich fen. Spatial and temporal variability in porewater chemistry across all peatland types highlights the importance of collecting porewater samples in multiples over time in both near-surface and deeper peats. Furthermore, this variation can be important when scaling findings to the peatland scale, assessing the representativeness of peatlands within a larger landscape, and understanding variability in solute export to downstream ecosystems.

54 ENVIRONMENTAL SCIENCES↗

AmeriFlux FLUXNET-1F CA-DB2 Delta Burns Bog 2

This is the AmeriFlux Management Project (AMP) created FLUXNET-1F version of the carbon flux data for the site CA-DB2 Delta Burns Bog 2. This is the FLUXNET version of the carbon flux data for the site CA-DB2 Delta Burns Bog 2 produced by applying the standard ONEFlux (1F) software. Site Description - The Burns Bog Ecological Conservancy Area (BBECA) in the metro Vancouver area presents a unique opportunity for insight into greenhouse gas dynamics post restoration. The bog was significantly disturbed for peat mining, development and agriculture over the last century, however since 2005 there has been both active restoration and natural regeneration occurring within the bog. Hence areas of bog are at varying stages of ecological recovery. This site is a Pine-Sphagnum low shrub site characteristic of a later successional stage. Conversely, CA-DBB, another AmeriFlux site in BBECA, is a Beakrush-Sphagnum site characteristic of an earlier successional stage.

Knox, Sara↗

SPRUCE Diurnal and Seasonal Patterns of Water Potential in S1 Bog and SPRUCE Experimental Plot Vegetation beginning in 2010

This data set reports on water potential from excised terminal shoots or leaves (Mainthemum only) of vegetation at the SPRUCE site located in the S1 bog. Pretreatment measurements were collected from outside plots within the S1 bog. Post treatments were collected from inside SPRUCE plots. Water potential was measured on the overstory canopy species, consisting of the evergreen Picea mariana (Mill.) B.S.P. (black spruce) and deciduous Larix laricina (Du Roi) K. Koch (tamarack) trees, and ericaceous shrubs < 1 m tall, consisting of the evergreen Rhododendron groenlandicum (Oeder) Kron & Judd (Labrador tea) and Chamaedaphne calyculata (L.) Moench. (leatherleaf). Some measurement campaigns also included the evergreen Kalmia polifolia, Wangenh. (bog laurel), the deciduous Vaccinium angustifolium (Aiton) (blueberry), and the herbaceous Maianthemum trifolium (L.) Sloboda (three-leaf false Solomon’s seal). This dataset contains one data file in comma-separated (*.csv) format. This is the second release of water potential data from the site with additional data from 2018 and 2019. These data cover 2010-06-22 to 2019-07-16. Further results will be added to this data set and released to the public periodically as quality assurance and publication of results are accomplished. There are 10 experimental plots in SPRUCE: five temperature treatments (+0, +2.25, +4.5, +6.75, +9°C) at ambient CO2, and the same five temperature treatments at elevated CO2 (+500 ppm). These data span the pre- and post-treatment periods when enclosed plots were exposed to warming and elevated carbon dioxide (CO2) within the SPRUCE experiment. The pretreatment samples were collected from south end of the S1 bog prior to SPRUCE boardwalk construction, then throughout the bog once the boardwalks were completed.

54 ENVIRONMENTAL SCIENCES↗

AmeriFlux CA-DB2 Delta Burns Bog 2

This is the AmeriFlux version of the carbon flux data for the site CA-DB2 Delta Burns Bog 2. Site Description - The Burns Bog Ecological Conservancy Area (BBECA) in the metro Vancouver area presents a unique opportunity for insight into greenhouse gas dynamics post restoration. The bog was significantly disturbed for peat mining, development and agriculture over the last century, however since 2005 there has been both active restoration and natural regeneration occurring within the bog. Hence areas of bog are at varying stages of ecological recovery. This site is a Pine-Sphagnum low shrub site characteristic of a later successional stage. Conversely, CA-DBB, another AmeriFlux site in BBECA, is a Beakrush-Sphagnum site characteristic of an earlier successional stage.

Knox, Sara↗

AmeriFlux FLUXNET-1F CA-SCB Scotty Creek Bog

This is the AmeriFlux Management Project (AMP) created FLUXNET-1F version of the carbon flux data for the site CA-SCB Scotty Creek Bog. This is the FLUXNET version of the carbon flux data for the site CA-SCB Scotty Creek Bog produced by applying the standard ONEFlux (1F) software. Site Description - The Scotty Creek bog flux tower is located in an organic-rich collapse-scar bog about 50 km south of Fort Simpson in the Taiga Plains of the Mackenzie watershed. The tower was installed in 2014 and operates an open-path EC system year-round running on solar power only. The collapse-scar bog is treeless and free of permafrost.

Sonnentag, Oliver↗

AmeriFlux CA-SCB Scotty Creek Bog

This is the AmeriFlux version of the carbon flux data for the site CA-SCB Scotty Creek Bog. Site Description - The Scotty Creek bog flux tower is located in an organic-rich collapse-scar bog about 50 km south of Fort Simpson in the Taiga Plains of the Mackenzie watershed. The tower was installed in 2014 and operates an open-path EC system year-round running on solar power only. The collapse-scar bog is treeless and free of permafrost.

Sonnentag, Oliver↗

SPRUCE Tritium in Porewater of SPRUCE Experimental Plots and Neighboring Peatlands, and in Groundwater, Stream Water, and Precipitation of the S1 Bog, 2014-2016

This data set reports the tritium concentrations in (1) porewater from selected SPRUCE experimental plots located in the S1 bog and in (2) groundwater, streamwater, and precipitation in the S1 bog watershed, and (3) in porewater from the S2 bog, S3 fen, and Bog Lake fen. All sites are located at the USDA Forest Service, Marcell Experimental Forest (MEF) near Grand Rapids, MN. Samples were collected between June of 2014 and August of 2016. These data include samples collected pre- and post-belowground warming treatments associated with the SPRUCE Experiment. There are 10 experimental plots in SPRUCE: 5 temperature treatments (+0, +2.25, +4.5, +6.75, +9°C) at ambient CO2, and the same 5 temperature treatments at elevated CO2 (+500 ppm). Porewater samples in this dataset were collected from three of the warming treatment plots (+0, +4.5, +9°C) at ambient CO2.

54 ENVIRONMENTAL SCIENCES↗

Unravelling biogeochemical drivers of methylmercury production in an Arctic fen soil and a bog soil

Arctic tundra soils store a globally significant amount of mercury (Hg), which could be transformed to the neurotoxic methylmercury (MeHg) upon warming and thus poses serious threats to the Arctic ecosystem. However, our knowledge of the biogeochemical drivers of MeHg production is limited in these soils. Using substrate addition (acetate and sulfate) and selective microbial inhibition approaches, we investigated the geochemical drivers and dominant microbial methylators in 60-day microcosm incubations with two tundra soils: a circumneutral fen soil and an acidic bog soil, collected near Nome, Alaska, United States. Results showed that increasing acetate concentration had negligible influences on MeHg production in both soils. However, inhibition of sulfate-reducing bacteria (SRB) completely stalled MeHg production in the fen soil in the first 15 days, whereas addition of sulfate in the low-sulfate bog soil increased MeHg production by 5-fold, suggesting prominent roles of SRB in Hg(II) methylation. Without the addition of sulfate in the bog soil or when sulfate was depleted in the fen soil (after 15 days), both SRB and methanogens contributed to MeHg production. Analysis of microbial community composition confirmed the presence of several phyla known to harbor microorganisms associated with Hg(II) methylation in the soils. Lastly, the observations suggest that SRB and methanogens were mainly responsible for Hg(II) methylation in these tundra soils, although their relative contributions depended on the availability of sulfate and possibly syntrophic metabolisms between SRB and methanogens.

54 ENVIRONMENTAL SCIENCES↗

AmeriFlux FLUXNET-1F AR-TF1 Rio Moat bog

This is the AmeriFlux Management Project (AMP) created FLUXNET-1F version of the carbon flux data for the site AR-TF1 Rio Moat bog. This is the FLUXNET version of the carbon flux data for the site AR-TF1 Rio Moat bog produced by applying the standard ONEFlux (1F) software. Site Description - Astelia pumila and Donatia fascicularis dominated cushion bog

Kutzbach, Lars↗

AmeriFlux US-TrS Trout Lake Area South Sparkling Bog

This is the AmeriFlux version of the carbon flux data for the site US-TrS Trout Lake Area South Sparkling Bog. Site Description - South Sparkling Bog was plowed after snow events during the winter of 2019/20 as an experimental treatment with Trout Bog (US-TrB) as a control

Stoy, Paul↗

AmeriFlux FLUXNET-1F CA-DBB Delta Burns Bog

This is the AmeriFlux Management Project (AMP) created FLUXNET-1F version of the carbon flux data for the site CA-DBB Delta Burns Bog. This is the FLUXNET version of the carbon flux data for the site CA-DBB Delta Burns Bog produced by applying the standard ONEFlux (1F) software. Site Description - Disturbed raised bog ecosystem (peat harvesting 1957 to 1963). Site has been restored and rewetted in 2007 by ditch blocking.

Christen, Andreas↗

AmeriFlux FLUXNET-1F US-BZB Bonanza Creek Thermokarst Bog

This is the AmeriFlux Management Project (AMP) created FLUXNET-1F version of the carbon flux data for the site US-BZB Bonanza Creek Thermokarst Bog. This is the FLUXNET version of the carbon flux data for the site US-BZB Bonanza Creek Thermokarst Bog produced by applying the standard ONEFlux (1F) software. Site Description - Intermediate to old age thermokarst collapse scar boreal bog in the Tanana Lowlands of Interior Alaska. Peat soils ~1-m thick. Recharged by precipitation and runoff from the surrounding forested peat plateaus. Primary wind direction in the summer is WSW.

Euskirchen, Eugenie↗

AmeriFlux FLUXNET-1F US-BZo Bonanza Creek Old Thermokarst Bog

This is the AmeriFlux Management Project (AMP) created FLUXNET-1F version of the carbon flux data for the site US-BZo Bonanza Creek Old Thermokarst Bog. This is the FLUXNET version of the carbon flux data for the site US-BZo Bonanza Creek Old Thermokarst Bog produced by applying the standard ONEFlux (1F) software. Site Description - Old thermokarst collapse scar boreal bog (several hundred years old) in the Tanana Lowlands of Interior Alaska. Peat soils ~1-m thick. Recharged by precipitation and runoff from the surrounding forested peat plateaus. Primary wind direction in the summer is WSW.

Euskirchen, Eugenie↗

AmeriFlux FLUXNET-1F AR-TF2 Rio Pipo bog

This is the AmeriFlux Management Project (AMP) created FLUXNET-1F version of the carbon flux data for the site AR-TF2 Rio Pipo bog. This is the FLUXNET version of the carbon flux data for the site AR-TF2 Rio Pipo bog produced by applying the standard ONEFlux (1F) software. Site Description - Pristine Sphagnum magellanicum dominated bog located in Parque Nacional Tierra del Fuego. The peatland extends about 60 ha.

Kutzbach, Lars [Universität Hamburg]↗

AmeriFlux FLUXNET-1F US-MBP Marcell Bog Lake Peatland

This is the AmeriFlux Management Project (AMP) created FLUXNET-1F version of the carbon flux data for the site US-MBP Marcell Bog Lake Peatland. This is the FLUXNET version of the carbon flux data for the site US-MBP Marcell Bog Lake Peatland produced by applying the standard ONEFlux (1F) software. Site Description - The study site is a fen within the Marcell Experimental Forest, which has been monitored for fluxes and environmental variables at various points in time. In 2007 an EC tower was established to monitor CO2/H2O fluxes within the peatland. Methane observations were added in 2009 and have been ongoing since. This site has also been referred to as Bog Lake Fen in the past.

Roman, Tyler [USDA Forest Service- International P↗

Investigating drivers of microbial activity and respiration in a forested bog

Northern peatlands store nearly one-third of terrestrial carbon (C) stocks while covering only 3% of the global landmass; nevertheless, the drivers of C cycling in these often-waterlogged ecosystems are different from those that control C dynamics in upland forested soils. To explore how multiple abiotic and biotic characteristics of bogs interact to shape microbial activity in a northern, forested bog, we added a labile C tracer ( 13 C-labeled starch) to in situ peat mesocosms and correlated heterotrophic respiration with natural variation in several microbial predictor variables, such as enzyme activity and microbial biomass, as well as with a suite of abiotic variables and proximity to vascular plants aboveground. We found that peat moisture content was positively correlated with respiration and microbial activity, even when moisture levels exceeded total saturation, suggesting that access to organic matter substrates in drier environments may be limiting for microbial activity. Proximity to black spruce trees decreased total and labile heterotrophic respiration. This negative relationship may reflect the influence of tree evapotranspiration and peat shading effects; i.e., microbial activity may decline as peat dries and cools near trees. Here, we isolated the response of heterotrophic respiration to explore the variation in, and interactions among, multiple abiotic and biotic drivers that influence microbial activity. This approach allowed us to reveal the relative influence of individual drivers on C respiration in these globally important C sinks.

59 BASIC BIOLOGICAL SCIENCES↗

Sequential Abiotic-Biotic Processes Drive Organic Carbon Transformation in Peat Bogs

Peatlands, which store one-third of the terrestrial carbon (C), are subject to large disturbances under a changing climate. It is crucial to understand how microbial and physiochemical factors affect the vulnerability of these large C stores to predict climate induced greenhouse gas fluxes. Here, we used a combination of mass spectrometry and spectroscopy techniques, to understand sequential biotic and abiotic degradation pathways of Sphagnum fallax leachate in an anaerobic incubation experiment, in the presence and absence of microorganisms. Removal of microorganisms was carried out by passing aqueous samples through 0.2 µm filters. In this work, our results revealed that Sphagnum fallax leachate degradation by abiotic reactions is a significant contributor to CO 2 production. Further, abiotic factors, such as low pH, are responsible for partial dissolved organic carbon (DOC) degradation that produces bioavailable compounds that shift microbial metabolic pathways and stimulate respiration in peat bogs. Acid-catalyzed hydrolysis of Sphagnum-produced glycosides can provide the microbial communities with glucose and stimulate microbial respiration of DOC to CO 2 . These results, while unique to peatlands, demonstrate the importance and underscore the complexity of sequential abiotic and biotic processes on C cycling in peat bogs. It is therefore crucial to incorporate abiotic degradation and sequential below-ground biotic and abiotic interactions into climate models for a better prediction of the influence of climate change on DOC stability in peatlands. These findings might not be representative of other ecosystems with different environmental conditions including mineral-rich peatlands and plant matter in surface peat horizons that comprise discrete microbial populations, and DOC composition.

54 ENVIRONMENTAL SCIENCES↗