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DOE OSTI2025

Bayesian estimation of HIV acquisition dates for prevention trials

Accurate timing estimates of when participants acquire HIV in HIV prevention trials are necessary for determining antibody levels at acquisition. The Antibody-Mediated Prevention (AMP) Studies showed that a passively administered broadly neutralizing antibody can prevent the acquisition of HIV from a neutralization-sensitive virus. We developed a pipeline for estimating the date of detectable HIV acquisition (DDA) in AMP Study participants using diagnostic and viral sequence data. Using a Bayesian strategy that combines three streams of data (REN [rev/vpu/env/Δnef] sequence, GP [gag/Δpol] sequence, and diagnostic) where their 95% credible intervals overlap based on pre-specified criteria and decision rules. We evaluated the performance of our AMP pipeline using PacBio viral sequence data from 41 participants across two prospective acute HIV acquisition cohort studies, FRESH and RV217, with twice-weekly sampling. These cohort studies enrolled young women in South Africa and men and women in Kenya and Thailand, respectively, with a high likelihood of HIV acquisition. In evaluating performance, “true DDA” was the center of bounds between last-negative and first-positive RNA diagnostic tests (median time 4 days, range 2–7 days); bias was the mean difference between estimated and true DDA. Using diagnostic data alone yielded timing estimates with a bias of 2.4 days and root mean square error (RMSE) of 7.9 days. These results were improved using sequence + diagnostic data (bias 1.5 days, RMSE 6.9 days), as well as by restricting sequence-based estimation to samples from ≤5 weeks post-DDA (bias 0.2 days, RMSE 7.8 days).

59 BASIC BIOLOGICAL SCIENCES↗

NASA NTRS2012

Obtaining Accurate Change Detection Results from High-Resolution Satellite Sensors

Multi-date acquisitions of high-resolution imaging satellites (e.g. GeoEye and WorldView), can display local changes of current economic interest. However, their large data volume precludes effective manual analysis, requiring image co-registration followed by image-to-image change detection, preferably with minimal analyst attention. We have recently developed an automatic change detection procedure that minimizes false-positives. The processing steps include: (a) Conversion of both the pre- and post- images to reflectance values (this step is of critical importance when different sensors are involved); reflectance values can be either top-of-atmosphere units or have full aerosol optical depth calibration applied using bi-directional reflectance knowledge. (b) Panchromatic band image-to-image co-registration, using an orthorectified base reference image (e.g. Digital Orthophoto Quadrangle) and a digital elevation model; this step can be improved if a stereo-pair of images have been acquired on one of the image dates. (c) Pan-sharpening of the multispectral data to assure recognition of change objects at the highest resolution. (d) Characterization of multispectral data in the post-image ( i.e. the background) using unsupervised cluster analysis. (e) Band ratio selection in the post-image to separate surface materials of interest from the background. (f) Preparing a pre-to-post change image. (g) Identifying locations where change has occurred involving materials of interest.

multispectral↗

NASA NTRS2011

Improvements in Speed and Functionality of a 670-GHz Imaging Radar

Significant improvements have been made in the instrument originally described in a prior NASA Tech Briefs article: Improved Speed and Functionality of a 580-GHz Imaging Radar (NPO-45156), Vol. 34, No. 7 (July 2010), p. 51. First, the wideband YIG oscillator has been replaced with a JPL-designed and built phase-locked, low-noise chirp source. Second, further refinements to the data acquisition and signal processing software have been performed by moving critical code sections to C code, and compiling those sections to Windows DLLs, which are then invoked from the main LabVIEW executive. This system is an active, single-pixel scanned imager operating at 670 GHz. The actual chirp signals for the RF and LO chains were generated by a pair of MITEQ 2.5 3.3 GHz chirp sources. Agilent benchtop synthesizers operating at fixed frequencies around 13 GHz were then used to up-convert the chirp sources to 15.5 16.3 GHz. The resulting signals were then multiplied 36 times by a combination of off-the-shelf millimeter- wave components, and JPL-built 200- GHz doublers and 300- and 600-GHz triplers. The power required to drive the submillimeter-wave multipliers was provided by JPL-built W-band amplifiers. The receive and transmit signal paths were combined using a thin, high-resistivity silicon wafer as a beam splitter. While the results at present are encouraging, the system still lacks sufficient speed to be usable for practical applications in a contraband detection. Ideally, an image acquisition speed of ten seconds, or a factor of 30 improvement, is desired. However, the system improvements to date have resulted in a factor of five increase in signal acquisition speed, as well as enhanced signal processing algorithms, permitting clearer imaging of contraband objects hidden underneath clothing. In particular, advances in three distinct areas have enabled these performance enhancements: base source phase noise reduction, chirp rate, and signal processing. Additionally, a second pixel was added, automatically reducing the imaging time by a factor of two. Although adding a second pixel to the system doubles the amount of submillimeter components required, some savings in microwave hardware can be realized by using a common low-noise source.

Dengler, Robert J.↗

NASA NTRS2010

Normalizing Landsat and ASTER Data Using MODIS Data Products for Forest Change Detection

Monitoring forest cover and its changes are a major application for optical remote sensing. In this paper, we present an approach to integrate Landsat, ASTER and MODIS data for forest change detection. Moderate resolution (10-100m) images (e.g. Landsat and ASTER) acquired from different seasons and times are normalized to one "standard" date using MODIS data products as reference. The normalized data are then used to compute forest disturbance index for forest change detection. Comparing to the results from original data, forest disturbance index from the normalized images is more consistent spatially and temporally. This work demonstrates an effective approach for mapping forest change over a large area from multiple moderate resolution sensors on various acquisition dates.

Gao, Feng↗

NASA NTRS1981

Laser velocimeter stand-alone controller

A distributed microprocessor network has been developed to position a laser-based velocity measurement package. Designed primarily for use inside the Ames 40 x 80-foot Subsonic Wind Tunnel, the instrument assembly is remotely operated. The mechanical system is reconfigurable for other test environments which necessitates an adaptive control approach. Three stepper motor drives provide two-dimensional positioning of the sampling point over 200/sq m of the test section, with a 5-mm resolution. Real-time multiprocessing provides fast response, fault detection, and parametric data acquisition. Manual tests are run from a dedicated control panel and automated tests are accomplished via an I.E.E.E 488 interface. The design approach and the progress to date are discussed.

Brown, R.↗

NASA NTRS1976

Landsat - A satellite surface water divining rod

A DAM (Detection and Mapping) package is developed to provide accurate up-to-date economical and properly formatted maps of surface water using Landsat earth resources satellite digital data in order to detect and locate unrecorded water impoundments. The operational procedure is discussed in terms of data acquisition, establishment of the control network, specification of map characteristics, and generation of maps. Preliminary evaluations essentially indicate that the DAM package can be readily used by personnel unfamiliar with computer processing of remote sensing data, that no false detections are encountered, and that detection accuracy of surface water impoundments greater than 4 hectares is between 95-98%. However, terrain shadows present a problem in mountainous areas at low sun angles. A total cost of less than 15 cents per sq mile to compile the inventory is noted.

Hancock, K. J.↗

NASA NTRS2017

Landsat-8 Operational Land Imager On-Orbit Radiometric Calibration

The Operational Land Imager (OLI), the VIS/NIR/SWIR sensor on the Landsat-8 has been successfully acquiring Earth Imagery for more than four years. The OLI incorporates two on-board radiometric calibration systems, one diffuser based and one lamp based, each with multiple sources. For each system one source is treated as primary and used frequently and the other source(s) are used less frequently to assist in tracking any degradation in the primary sources. In addition, via a spacecraft maneuver, the OLI instrument views the moon once a lunar cycle (approx. 29 days). The integrated lunar irradiances from these acquisitions are compared to the output of a lunar irradiance model. The results from all these techniques, combined with cross calibrations with other sensors and ground based vicarious measurements are used to monitor the OLI's stability and correct for any changes observed. To date, the various techniques have other detected significant changes in the shortest wavelength OLI band centered at 443 nm and these are currently being adjusted in the operational processing.

radiometric calibratio↗

NASA NTRS2024

Future of the Search for Life: Workshop Report

The 2-week, virtual Future of the Search for Life science and engineering workshop brought together more than 100 scientists, engineers, and technologists in March and April 2022 to provide their expert opinion on the interconnections between life-detection science and technology. Participants identified the advances in measurement and sampling technologies they believed to be necessary to perform in situ searches for life elsewhere in our Solar System, 20 years or more in the future. Among suggested measurements for these searches, those pertaining to three potential indicators of life termed “dynamic disequilibrium,” “catalysis,” and “informational polymers” were identified as particularly promising avenues for further exploration. For these three indicators, small breakout groups of participants identified measurement needs and knowledge gaps, along with corresponding constraints on sample handling (acquisition and processing) approaches for a variety of environments on Enceladus, Europa, Mars, and Titan. Despite the diversity of these environments, sample processing approaches all tend to be more complex than those that have been implemented on missions or envisioned for mission concepts to date. The approaches considered by workshop breakout groups progress from nondestructive to destructive measurement techniques, and most involve the need for fluid (especially liquid) sample processing. Sample processing needs were identified as technology gaps. These gaps include technology and associated sampling strategies that allow the preservation of the thermal, mechanical, and chemical integrity of the samples upon acquisition; and to optimize the sample information obtained by operating suites of instruments on common samples. Crucially, the interplay between science-driven life-detection strategies and their technological implementation highlights the need for an unprecedented level of payload integration and extensive collaboration between scientists and engineers, starting from concept formulation through mission deployment of life-detection instruments and sample processing systems.

Marc Neveu↗

NASA NTRS1976

Data acquisition and path selection decision making for an autonomous roving vehicle

Problems related to the guidance of an autonomous rover for unmanned planetary exploration were investigated. Topics included in these studies were: simulation on an interactive graphics computer system of the Rapid Estimation Technique for detection of discrete obstacles; incorporation of a simultaneous Bayesian estimate of states and inputs in the Rapid Estimation Scheme; development of methods for estimating actual laser rangefinder errors and their application to date provided by Jet Propulsion Laboratory; and modification of a path selection system simulation computer code for evaluation of a hazard detection system based on laser rangefinder data.

Frederick, D. K.↗

NASA NTRS1974

Automated thematic mapping and change detection of ERTS-1 images

A system that inventories and updates resources must be capable of recognizing resources and their changes rapidly, using imagery acquired by a resources satellite such as ERTS-1. The conversion of ERTS images to thematic maps showing the distribution of resources is the first step in the data reduction process. To accomplish this task, the resources must be recognized from spatial and multispectral signatures. In addition, resource boundaries must be accurately established, and the data from different acquisition dates must be registered. This paper describes a system that combines multispectral and spatial pattern recognition techniques to produce thematic maps. This system has been applied to ERTS-1 MSS images, and the results obtained are discussed.

Gramenopoulos, N.↗

NASA NTRS2011

Helical Face Gear Development Under the Enhanced Rotorcraft Drive System Program

U.S. Army goals for the Enhanced Rotorcraft Drive System Program are to achieve a 40 percent increase in horsepower to weight ratio, a 15 dB reduction in drive system generated noise, 30 percent reduction in drive system operating, support, and acquisition cost, and 75 percent automatic detection of critical mechanical component failures. Boeing s technology transition goals are that the operational endurance level of the helical face gearing and related split-torque designs be validated to a TRL 6, and that analytical and manufacturing tools be validated. Helical face gear technology is being developed in this project to augment, and transition into, a Boeing AH-64 Block III split-torque face gear main transmission stage, to yield increased power density and reduced noise. To date, helical face gear grinding development on Northstar s new face gear grinding machine and pattern-development tests at the NASA Glenn/U.S. Army Research Laboratory have been completed and are described.

Heath, Gregory F.↗

DOE OSTI2021

New Orthophoto Generation Strategies from UAV and Ground Remote Sensing Platforms for High-Throughput Phenotyping

Remote sensing platforms have become an effective data acquisition tool for digital agriculture. Imaging sensors onboard unmanned aerial vehicles (UAVs) and tractors are providing unprecedented high-geometric-resolution data for several crop phenotyping activities (e.g., canopy cover estimation, plant localization, and flowering date identification). Among potential products, orthophotos play an important role in agricultural management. Traditional orthophoto generation strategies suffer from several artifacts (e.g., double mapping, excessive pixilation, and seamline distortions). The above problems are more pronounced when dealing with mid- to late-season imagery, which is often used for establishing flowering date (e.g., tassel and panicle detection for maize and sorghum crops, respectively). In response to these challenges, this paper introduces new strategies for generating orthophotos that are conducive to the straightforward detection of tassels and panicles. The orthophoto generation strategies are valid for both frame and push-broom imaging systems. The target function of these strategies is striking a balance between the improved visual appearance of tassels/panicles and their geolocation accuracy. The new strategies are based on generating a smooth digital surface model (DSM) that maintains the geolocation quality along the plant rows while reducing double mapping and pixilation artifacts. Moreover, seamline control strategies are applied to avoid having seamline distortions at locations where the tassels and panicles are expected. The quality of generated orthophotos is evaluated through visual inspection as well as quantitative assessment of the degree of similarity between the generated orthophotos and original images. Several experimental results from both UAV and ground platforms show that the proposed strategies do improve the visual quality of derived orthophotos while maintaining the geolocation accuracy at tassel/panicle locations.

54 ENVIRONMENTAL SCIENCES↗

DOE OSTI2023

Video-rate Raman-based metabolic imaging by Airy light-sheet illumination and photon-sparse detection

Despite its massive potential, Raman imaging represents just a modest fraction of all research and clinical microscopy to date. This is due to the ultralow Raman scattering cross-sections of most biomolecules that impose low-light or photon-sparse conditions. Bioimaging under such conditions is suboptimal, as it either results in ultralow frame rates or requires increased levels of irradiance. Here, we overcome this tradeoff by introducing Raman imaging that operates at both video rates and 1,000-fold lower irradiance than state-of-the-art methods. To accomplish this, we deployed a judicially designed Airy light-sheet microscope to efficiently image large specimen regions. Further, we implemented subphoton per pixel image acquisition and reconstruction to confront issues arising from photon sparsity at just millisecond integrations. We demonstrate the versatility of our approach by imaging a variety of samples, including the three-dimensional (3D) metabolic activity of single microbial cells and the underlying cell-to-cell variability. To image such small-scale targets, we again harnessed photon sparsity to increase magnification without a field-of-view penalty, thus, overcoming another key limitation in modern light-sheet microscopy.

60 APPLIED LIFE SCIENCES↗

DOE OSTI2025

Development and validation of a software for simulating γ-γ coincidence emission and detection probabilities

Gamma-gamma coincidence spectrometers have the potential to significantly enhance detection sensitivity for ultra-trace radionuclide measurements. The implementation of these spectrometers, however, is limited by the complexity of acquisition hardware, data processing and quantification. This work reports development of a novel radionuclide quantification software for γ-γ coincidence measurements. For any radionuclide, the software parses the Evaluated Nuclear Structure Data File (ENSDF) database, recursively simulating all possible γ-γ coincidence signatures and their respective emission and detection probabilities. Implemented using Python programming language, the software employs several strategies to boost overall computational performance. Since coincidence-based spectrometers are of notable interest in monitoring compliance for the Comprehensive Nuclear-Test-Ban Treaty (CTBT), the software’s execution was tested for 84 CTBT-relevant radionuclides. To date, the software has been experimentally validated for 15 radionuclides using the Advanced Radionuclide Gamma spectrOmeter (ARGO) at Pacific Northwest National Laboratory, USA (PNNL). Notably, the software can be operated in convergence mode, whereby coincidence detection efficiency’s convergence behavior can help avoid unreliable radionuclide activity estimates. With growing number of coincidence spectrometers worldwide, this paper aims to assist the radiation metrology community in developing similar software for their system.

46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND ↗

NASA NTRS1975

Real-time detection and data acquisition system for the left ventricular outline

The paper describes the design and capabilities of a computer-interfaced angiocardiographic automated border recognizer for real-time detection and data acquisition regarding the left ventricular outline. The contour detection is based on a thresholding technique that uses an analog comparator to compare the video signal with a constant reference level, which can be used satisfactorily only if the brightness level is roughly constant along a ventricular border. The use of dynamic reference level is described for achieving a marked improvement by having the reference level dynamically adjusted according to local brightness levels on a line-to-line basis. Also discussed are the features of the computer interface designed and implemented for the real-time on-line storage of the obtained border coordinates. Results to date indicate that the system provides an accurate left ventricular contour even in pictures with relatively low contrast. Application of the threshold detection technique to echocardiography is briefly discussed.

Reiber, J. H. C.↗

NASA NTRS2018

On-Orbit Performance & Operation of the Attitude & Pointing Control Subsystems on ASTERIA

The Arcsecond Space Telescope Enabling Research in Astrophysics (ASTERIA) is a 6U CubeSat that was deployed from the International Space Station on 20 November 2017. The underlying goal of the mission is to image and perform photometry on bright, nearby stars and possibly detect transiting exoplanets orbiting these stars. As a technology demonstration with an eye to enable this science, the payload must be pointed with a stability of 5 arcseconds RMS over 20-minute observations and a repeatability of 1 arcsecond RMS across multiple observations. A two-stage control system was employed to achieve these pointing requirements: reaction wheels control the attitude of the spacecraft bus while a piezo stage translates the focal plane array to control the pointing of the payload. This paper will present on-orbit results that demonstrate a pointing stability of 0.5 arcsecond RMS over 20 minutes and a pointing repeatability of 1 milliarcsecond RMS from observation to observation, the best pointing of a CubeSat to date. In addition, this paper will discuss the pointing achieved by the attitude control subsystem alone, pointing issues due to temporarily bright pixels, hot pixels, and reaction wheel speed reversals or zero crossings, the deployment and Sun acquisition phase, momentum management issues arising from a large residual dipole, and some of the anomalies encountered with the attitude control subsystem.

Pong, Christopher M.↗

NASA NTRS2000

Improving Cloud Detection in Satellite Images of Coral Reef Environments Using Space Shuttle Photographs and High-Definition Television

Coral reefs worldwide are suffering from severe and rapid degradation (Bryant et A, 1998; Hoegh-Guldberg, 1999). Quick, consistent, large-scale assessment is required to assess and monitor their status (e.g., USDOC/NOAA NESDIS et al., 1999). On-going systematic collection of high resolution digital satellite data will exhaustively complement the relatively small number of SPOT, Landsat 4-5, and IRS scenes acquired for coral reefs the last 20 years. The workhorse for current image acquisition is the Landsat 7 ETM+ Long Term Acquisition Plan (Gasch et al. 2000). Coral reefs are encountered in tropical areas and cloud contamination in satellite images is frequently a problem (Benner and Curry 1998), despite new automated techniques of cloud cover avoidance (Gasch and Campana 2000). Fusion of multidate acquisitions is a classical solution to solve the cloud problems. Though elegant, this solution is costly since multiple images must be purchased for one location; the cost may be prohibitive for institutions in developing countries. There are other difficulties associated with fusing multidate images as well. For example, water quality or surface state can significantly change through time in coral reef areas making the bathymetric processing of a mosaiced image strenuous. Therefore, another strategy must be selected to detect clouds and improve coral reefs mapping. Other supplemental data could be helpful and cost-effective for distinguishing clouds and generating the best possible reef maps in the shortest amount of time. Photographs taken from the 1960s to the present from the Space Shuttle and other human-occupied spacecraft are one under-used source of alternative multitemporal data (Lulla et al. 1996). Nearly 400,000 photographs have been acquired during this period, an estimated 28,000 of these taken to date are of potential value for reef remote sensing (Robinson et al. 2000a). The photographic images can be digitized into three bands (red, green and blue) and processed for various applications (e.g., Benner and Curry 1998, Nedeltchev 1999, Glasser and Lulla 2000, Robinson et al. 2000c, Webb et al, in press).

Andrefeouet, Serge↗

NASA NTRS2020

SAGE III ISS Contamination Monitoring Package: Observations in Orbit

The Stratospheric Aerosol and Gas Experiment III (SAGE III) telescope and instrument assembly employ the methods of solar occultation and lunar occultation to retrieve near-global vertical profiles of atmospheric ozone, water vapor, nitrogen dioxide, aerosol extinctions, and other gaseous species and atmospheric state parameters. The SAGE III grating spectrometer measures light within the spectral range of 280 nm to 1037 nm at approximately 1 nm resolution, but retrievals in the Ultraviolet (UV) are particularly sensitive to contamination of the optical train. Therefore, a contamination door that contains a quartz optical window can be closed over the telescope aperture during periods of enhanced external contaminant flux. This optically transparent window permits continued science event acquisition at an acceptably diminished signal-to-noise ratio, which is expected to decline with ongoing accretion of contaminant material. To date, this impact has been short term, and science quality through the window returns to baseline performance after a contamination source is removed and spontaneous desorption removes material from the low-affinity quartz surface. Two Contamination Monitoring Packages (CMPs) consisting of eight Thermoelectric Quartz Crystal Microbalances (TQCMs) from QCM Research provide characterization and redundant monitoring of contaminant deposition from the 2π steradian solid angle on the payload side of the Expedite the Processing of Experiments to the Space Station (ExPRESS) Payload Adapter. CMP data are closely examined by the SAGE III team to determine when the contamination door should remain closed during science events and in what direction the instrument assembly scan head should stow when not acquiring science measurements. Additionally, should the CMPs indicate an unacceptable accretion rate, the ight computer will close the contamination door as part of the automatic fault detection system. Along with spectrometer measurements of the quartz window's transmission, the payload CMPs enable auditing of the mission contamination budget. The process of Thermogravimetric Analysis (TGA) can be used to help identify chemical constituents accreted on the CMP sensors. To be presented here along with an explanation of the CMP systems are the first two and a half years of observations of the contaminant deposition environment around the payload in quiescence and during special events like docking vehicles.

Tyler T Dawson↗