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Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

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PCR Based Microbial Monitor for Analysis of Recycled Water Aboard the ISSA: Issues and Prospects

The monitoring of spacecraft life support systems for the presence of health threatening microorganisms is paramount for crew well being and successful completion of missions. Development of technology to monitor spacecraft recycled water based on detection and identification of the genetic material of contaminating microorganisms and viruses would be a substantial improvement over current NASA plans to monitor recycled water samples that call for the use of conventional microbiology techniques which are slow, insensitive, and labor intensive. The union of the molecular biology techniques of DNA probe hybridization and polymerase chain reaction (PCR) offers a powerful method for the detection, identification, and quantification of microorganisms and viruses. This technology is theoretically capable of assaying samples in as little as two hours with specificity and sensitivity unmatched by any other method. A major advance in probe-hybridization/PCR has come about in a technology called TaqMan(TM), which was invented by Perkin Elmer. Instrumentation using TaqMan concepts is evolving towards devices that could meet NASA's needs of size, low power use, and simplicity of operation. The chemistry and molecular biology needed to utilize these probe-hybridization/PCR instruments must evolve in parallel with the hardware. The following issues of chemistry and biology must be addressed in developing a monitor: Early in the development of a PCR-based microbial monitor it will be necessary to decide how many and which organisms does the system need the capacity to detect. We propose a set of 17 different tests that would detect groups of bacteria and fungus, as well as specific eukaryotic parasites and viruses; In order to use the great sensitivity of PCR it will be necessary to concentrate water samples using filtration. If a lower limit of detection of 1 microorganism per 100 ml is required then the microbes in a 100 ml sample must be concentrated into a volume that can be added to a PCR assay; There are not likely to be contaminants in ISSA recycled water that would inhibit PCR resulting in false-negative results; The TaqMan PCR product detection system is the most promising method for developing a rapid, highly automated gene-based microbial monitoring system. The method is inherently quantitative. NASA and other government agencies have invested in other technologies that, although potentially could lead to revolutionary advances, are not likely to mature in the next 5 years into working systems; PCR-based methods cannot distinguish between DNA or RNA of a viable microorganism and that of a non-viable organism. This may or may not be an important issue with reclaimed water on the ISSA. The recycling system probably damages the capacity of the genetic material of any bacteria or viruses killed during processing to serve as a template in a PCR desinged to amplify a large segment of DNA (less than 650 base pairs). If necessary, vital dye staining could be used in addition to PCR, to enumerate the viable cells in a water sample; The quality control methods have been developed to insure that PCR's are working properly, and that reactions are not contaminated with PCR carryover products which could lead to the generation of false-positive results; and The sequences of the small rRNA subunit gene for a large number of microorganisms are known, and they consititue the best database for rational development of the oligonucleotide reagents that give PCR its great specificity. From those gene sequences, sets of oligonucleotide primers for PCR and Taqman detection that could be used in a NASA microbial monitor were constructed using computer based methods. In addition to space utilization, a microbial monitior will have tremendous terrestrial applications. Analysis of patient samples for microbial pathogens, testing industrial effluent for biofouling bacteria, and detection biological warfare agents on the battlefield are but a few of the diverse potential uses for this technology. Once fully developed, gene-based microbial monitors will become the fundamental tool in every lab that tests for microbial contaminants, and serve as a powerful weapon in mankind's war with the germ world.

Cassell, Gail H.↗

Systems engingeering for the Kepler Mission : a search for terrestrial planets

The Kepler mission will launch in 2007 and determine the distribution of earth-size planets (0.5 to 10 earth masses) in the habitable zones (HZs) of solar-like stars. The mission will monitor > 100,000 dwarf stars simultaneously for at least 4 years. Precision differential photometry will be used to detect the periodic signals of transiting planets. Kepler will also support asteroseismology by measuring the pressure-mode (p-mode) oscillations of selected stars. Key mission elements include a spacecraft bus and 0.95 meter, wide-field, CCD-based photometer injected into an earth-trailing heliocentric orbit by a 3-stage Delta II launch vehicle as well as a distributed Ground Segment and Follow-up Observing Program. The project is currently preparing for Preliminary Design Review (October 2004) and is proceeding with detailed design and procurement of long-lead components. In order to meet the unprecedented photometric precision requirement and to ensure a statistically significant result, the Kepler mission involves technical challenges in the areas of photometric noise and systematic error reduction, stability, and false-positive rejection. Programmatic and logistical challenges include the collaborative design, modeling, integration, test, and operation of a geographically and functionally distributed project. A very rigorous systems engineering program has evolved to address these challenges. This paper provides an overview of the Kepler systems engineering program, including some examples of our processes and techniques in areas such as requirements synthesis, validation & verification, system robustness design, and end-to-end performance modeling.

systems engineering↗

Support for Systematic Code Reviews with the SCRUB Tool

SCRUB is a code review tool that supports both large, team-based software development efforts (e.g., for mission software) as well as individual tasks. The tool was developed at JPL to support a new, streamlined code review process that combines human-generated review reports with program-generated review reports from a customizable range of state-of-the-art source code analyzers. The leading commercial tools include Codesonar, Coverity, and Klocwork, each of which can achieve a reasonably low rate of false-positives in the warnings that they generate. The time required to analyze code with these tools can vary greatly. In each case, however, the tools produce results that would be difficult to realize with human code inspections alone. There is little overlap in the results produced by the different analyzers, and each analyzer used generally increases the effectiveness of the overall effort. The SCRUB tool allows all reports to be accessed through a single, uniform interface (see figure) that facilitates brows ing code and reports. Improvements over existing software include significant simplification, and leveraging of a range of commercial, static source code analyzers in a single, uniform framework. The tool runs as a small stand-alone application, avoiding the security problems related to tools based on Web browsers. A developer or reviewer, for instance, must have already obtained access rights to a code base before that code can be browsed and reviewed with the SCRUB tool. The tool cannot open any files or folders to which the user does not already have access. This means that the tool does not need to enforce or administer any additional security policies. The analysis results presented through the SCRUB tool s user interface are always computed off-line, given that, especially for larger projects, this computation can take longer than appropriate for interactive tool use. The recommended code review process that is supported by the SCRUB tool consists of three phases: Code Review, Developer Response, and Closeout Resolution. In the Code Review phase, all tool-based analysis reports are generated, and specific comments from expert code reviewers are entered into the SCRUB tool. In the second phase, Developer Response, the developer is asked to respond to each comment and tool-report that was produced, either agreeing or disagreeing to provide a fix that addresses the issue that was raised. In the third phase, Closeout Resolution, all disagreements are discussed in a meeting of all parties involved, and a resolution is made for all disagreements. The first two phases generally take one week each, and the third phase is concluded in a single closeout meeting.

Holzmann, Gerald J.↗

Follow-up Imaging of Disk Candidates from the Disk Detective Citizen Science Project: New Discoveries and False Positives in WISE Circumstellar Disk Surveys

The Disk Detective citizen science project aims to find new stars with excess 22 m emission from circumstellar dust in the All WISE data release from the Wide-field Infrared Survey Explorer. We evaluated 261 Disk Detective objects of interest with imaging with the Robo-AO adaptive optics instrument on the 1.5 m telescope at Palomar Observatory and with RetroCam on the 2.5 m du Pont Telescope at Las Campanas Observatory to search for background objects at 0 15–12 separations from each target. Our analysis of these data leads us to reject 7% of targets. Combining this result with statistics from our online image classification efforts implies that at most7.9%±0.2% of All WISE-selected infrared excesses are good disk candidates. Applying our false-positive rates to other surveys, we find that the infrared excess searches of McDonald et al. and Marton et al. all have false-positiverates >70%. Moreover, we find that all 13 disk candidates in Theissen & West with W4 signal-to-noise ratio >3are false positives. We present 244 disk candidates that have survived vetting by follow-up imaging. Of these,213 are newly identified disk systems. Twelve of these are candidate members of comoving pairs based on Gaia astrometry, supporting the hypothesis that warm dust is associated with binary systems. We also note the discovery of 22 m excess around two known members of the Scorpius–Centaurus association, and we identifyknown disk host WISEA J164540.79-310226.6 as a likely Sco-Cen member. Thirty of these disk candidates arecloser than 125 pc (including 26 debris disks), making them good targets for both direct-imaging exoplanetsearches.

Wide-field Infrared Survey Explorer↗

Numerical solutions for viscous and potential flow about arbitrary two-dimensional bodies using body-fitted coordinate systems

A procedure for numerical solution of the time-dependent, incompressible Navier-Stokes equations for the flow about arbitrarily shaped two-dimensional bodies is given. This solution is based on a technique of automatic numerical generation of a curvilinear coordinate system having a coordinate line coincident with the body contour regardless of its shape. The implicit solution utilizes the vorticity-stream function formulation with a false-position iterative adjustment of the surface vorticity in satisfaction of the no-slip boundary condition. Excellent agreement with the Blasius boundary layer solution is obtained for a semi-infinite flat plate. Results are presented for Reynolds numbers up to 2000 for several airfoils and a cambered rock.

Thames, F. C.↗

Testing Saliency Parameters for Automatic Target Recognition

A bottom-up visual attention model (the saliency model) is tested to enhance the performance of Automated Target Recognition (ATR). JPL has developed an ATR system that identifies regions of interest (ROI) using a trained OT-MACH filter, and then classifies potential targets as true- or false-positives using machine-learning techniques. In this project, saliency is used as a pre-processing step to reduce the space for performing OT-MACH filtering. Saliency parameters, such as output level and orientation weight, are tuned to detect known target features. Preliminary results are promising and future work entails a rigrous and parameter-based search to gain maximum insight about this method.

regions of interest (ROI)↗

Diagnostic accuracy of an ultrasonic multiple transducer cardiac imaging system

An ultrasonic multiple-transducer imaging system for intracardiac structure visualization is developed in order to simplify visualization of the human heart in vivo without radiation hazard or invasion of the body. Results of the evaluation of the diagnostic accuracy of the devised system in a clinical setting for adult patients are presented and discussed. Criteria are presented for recognition of mitral valva prolapse, mitral stenosis, pericardial effusion, atrial septal defect, and left ventricular dyssynergy. The probable cause for false-positive and false-negative diagnoses is discussed. However, hypertrophic myopathy and congestive myopathy were unable to be detected. Since only qualitative criteria were used, it was not possible to differentiate patients with left ventricular volume overload from patients without cardiac pathology.

Popp, R. L.↗

The NANOGrav 11 yr data set: Evolution of Gravitational-wave Background Statistics

An ensemble of inspiraling supermassive black hole binaries should produce a stochastic background of very low frequency gravitational waves. This stochastic background is predicted to be a power law, with a gravitational-wave strain spectral index of −2/3, and it should be detectable by a network of precisely timed millisecond pulsars, widely distributed on the sky. This paper reports a new “time slicing” analysis of the 11 yr data release from the North American Nanohertz Observatory for Gravitational Waves (NANOGrav) using 34 millisecond pulsars. Methods to flag potential “false-positive” signatures are developed, including techniques to identify responsible pulsars. Mitigation strategies are then presented. We demonstrate how an incorrect noise model can lead to spurious signals, and we show how independently modeling noise across 30 Fourier components, spanning NANOGrav’s frequency range, effectively diagnoses and absorbs the excess power in gravitational-wave searches. This results in a nominal, and expected, progression of our gravitational-wave statistics. Additionally, we show that the first interstellar medium event in PSR J1713+0747 pollutes the common red-noise process with low spectral index noise, and we use a tailored noise model to remove these effects.

J S Hazboun↗

Obtaining Accurate Change Detection Results from High-Resolution Satellite Sensors

Multi-date acquisitions of high-resolution imaging satellites (e.g. GeoEye and WorldView), can display local changes of current economic interest. However, their large data volume precludes effective manual analysis, requiring image co-registration followed by image-to-image change detection, preferably with minimal analyst attention. We have recently developed an automatic change detection procedure that minimizes false-positives. The processing steps include: (a) Conversion of both the pre- and post- images to reflectance values (this step is of critical importance when different sensors are involved); reflectance values can be either top-of-atmosphere units or have full aerosol optical depth calibration applied using bi-directional reflectance knowledge. (b) Panchromatic band image-to-image co-registration, using an orthorectified base reference image (e.g. Digital Orthophoto Quadrangle) and a digital elevation model; this step can be improved if a stereo-pair of images have been acquired on one of the image dates. (c) Pan-sharpening of the multispectral data to assure recognition of change objects at the highest resolution. (d) Characterization of multispectral data in the post-image ( i.e. the background) using unsupervised cluster analysis. (e) Band ratio selection in the post-image to separate surface materials of interest from the background. (f) Preparing a pre-to-post change image. (g) Identifying locations where change has occurred involving materials of interest.

multispectral↗

Kepler Planet Detection Mission: Introduction and First Results

The Kepler Mission is designed to determine the frequency of Earth-size and rocky planets in and near the habitable zone (HZ) of solar-like stars. The HZ is defined to be the region of space where a rocky planet could maintain liquid water on its surface. Kepler is the 10th competitively-selected Discovery Mission and was launched on March 6, 2009. Since completing its commissioning, Kepler has observed over 156,000 stars simultaneously and near continuously to search for planets that periodically pass in front of their host star (transit). The photometric precision is approximately 23 ppm for 50% of the 12th magnitude dwarf stars for an integration period of 6.5 hours. During the first 3 months of operation the photometer detected transit-like signatures from more than 200 stars. Careful examination shows that many of these events are false-positives such as small stars orbiting large stars or blends of target stars with eclipsing binary stars. Ground-based follow-up observations confirm the discovery of five new exoplanets with sizes between 0.37 andl.6 Jupiter radii (R(sub J)) and orbital periods ranging from 3.2 to 4.9 days. Ground-based observations with the Keck 1, Hobby-Ebberly, Hale, WIYN, MMT, Tillinghast, Shane, and Nordic Optical Telescopes are used to vet the planetary candidates and measure the masses of the putative planets. Observations of occultations and phase variations of hot, short-period planets such as HT-P-7b provide a probe of atmospheric properties. Asteroseismic analysis already shows the presence of p-mode oscillations in several stars. Such observations will be used to measure the mean stellar density and infer the stellar size and age. For stars too dim to permit asteroseismology, observations of the centroid motion of target stars will be used to measure the parallax and be combined with photometric measurements to estimate stellar sizes. Four open clusters are being observed to determine stellar rotation rates as a function of age and spectral type. Many different types of stellar variability are observed with unprecedented precision and over a wide range of time scales. Solar-like photometric variability of thousands of field stars is being studied to determine how photometric variability and the stellar rotation rates change with stellar age and metallicity. A wide variety of other astrophysical phenomena have also been observed. The data are being analyzed at the Ames Research Center and archived at the MAST at STScI. The Kepler Mission also supports a vigorous Guest Observer Program.

Borucki, William↗

Performance Evaluation of a Shaped Sonic Boom Detector and Classifier

NASA will soon fly the X‑59 aircraft over selected communities to evaluate community responses to shaped sonic booms. Community tests will include dozens of deployed acoustic sensors capable of measuring and detecting shaped sonic boom waveforms in situ for rapid onboard analysis. To this end, we present a sonic boom detector and classifier. The sonic boom detector identifies a shaped sonic boom within a measured acoustical waveform by calculating the cross-correlation with a template shaped sonic boom waveform. The sonic boom classifier determines whether the identified event is indeed a shaped sonic boom based on the correlation coefficient and the calculated noise exposure level. We evaluate these algorithms using simulations of on- and off-design X-59 sonic boom waveforms injected into previously measured 30 s ambient noise recordings. Results of this case study indicate that the detector identifies a sonic boom with an accuracy of ±100 ms in 99.98% of the cases. Furthermore, for a given 30 s measurement, the classifier shows true-positive rates of approximately 0.9999 when the false-positive rate is 10e-3. The case study demonstrates that the recommended onboard sonic boom detector and classifier should be highly capable of identifying shaped sonic booms.

sonic boom↗