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At least 19 records

Ligand‐induced CaMKIIα hub Trp403 flip, hub domain stacking, and modulation of kinase activity

Abstract γ‐Hydroxybutyric acid (GHB) analogs are small molecules that bind competitively to a specific cavity in the oligomeric CaMKIIα hub domain. Binding affects conformation and stability of the hub domain, which may explain the neuroprotective action of some of these compounds. Here, we describe molecular details of interaction of the larger‐type GHB analog 2‐(6‐(4‐chlorophenyl)imidazo[1,2‐b]pyridazine‐2‐yl)acetic acid (PIPA). Like smaller‐type analogs, PIPA binding to the CaMKIIα hub domain promoted thermal stability. PIPA additionally modulated CaMKIIα activity under sub‐maximal CaM concentrations and ultimately led to reduced substrate phosphorylation. A high‐resolution X‐ray crystal structure of a stabilized CaMKIIα (6x mutant) hub construct revealed details of the binding mode of PIPA, which involved outward placement of tryptophan 403 (Trp403), a central residue in a flexible loop close to the upper hub cavity. Small‐angle X‐ray scattering (SAXS) solution structures and mass photometry of the CaMKIIα wild‐type hub domain in the presence of PIPA revealed a high degree of ordered self‐association (stacks of CaMKIIα hub domains). This stacking neither occurred with the smaller compound 3‐hydroxycyclopent‐1‐enecarboxylic acid (HOCPCA), nor when Trp403 was replaced with leucine (W403L). Additionally, CaMKIIα W403L hub was stabilized to a larger extent by PIPA compared to CaMKIIα hub wild type, indicating that loop flexibility is important for holoenzyme stability. Thus, we propose that ligand‐induced outward placement of Trp403 by PIPA, which promotes an unforeseen mechanism of hub domain stacking, may be involved in the observed reduction in CaMKIIα kinase activity. Altogether, this sheds new light on allosteric regulation of CaMKIIα activity via the hub domain.

Biochemistry & Molecular Biology

Best Practices for Resilience Hub Development and Management

The Carbon League and its community partners in East St. Louis, Illinois, have identified five facilities to serve as resilience hubs. These hubs are intended to support the local community through a range of services and resources during blue-sky (everyday), gray-sky (pre-event), and black-sky (emergency) conditions. Transforming these facilities into fully functional resilience hubs requires a broad operational improvement and programmatic planning roadmap. This memo outlines best practices to guide the development of these resilience hubs in East St. Louis, including recommendations for infrastructure services; safety and physical protection; community services; operational protocols; and a phased implementation strategy aligned with realistic funding and capacity constraints. Infrastructure recommendations include strengthening electric power, communications, water, sanitation, and transportation/logistics capabilities, all of which are essential for hubs that may serve as cooling and warming centers, distribution points, and information hubs during emergencies. Safety recommendations focus on accessibility, emergency action planning, indoor air quality, and secure storage of critical equipment. A phased roadmap provides guidance from immediate, low-cost readiness actions to long-term optimization and community integration. Performance metrics and maintenance protocols ensure continuous improvement and operational readiness. This guidance draws on best practices that can be used to support the development of resilient, community-centered hubs capable of enhancing public safety, health, and well-being during everyday operations and emergencies alike.

99 GENERAL AND MISCELLANEOUS

Illinois Basin Regional DAC Hub TA-1: Feasibility (Phase-0) (Final Scientific/Technical Report)

The overall objective of this project was to complete a feasibility study for a Regional Direct Air Capture (DAC) Hub that encompasses Illinois, Indiana, and Kentucky. The geographic construct of this hub was based on the Illinois Basin – a geological area where at least seven (7) geological storage studies were conducted. The Hub was designed to assure a capacity to capture, store, and utilize at least 1,000,000 metric tons (tonnes) of CO2 from the atmosphere annually 1 metric tonnes per annum (MTA), starting from an initial capacity of 200,000 tonnes of CO2 per annum (KTA). The Hub also included:(1) clean energy providers to supply renewable energy to the DAC units; (2) geological storage facilities to inject and sequestrate the captured CO2; (3) CO2 utilization facilities that would uptake the captured CO2 and convert it into products; and (4) small business organizations that would assist in workforce development efforts for the region. This closeout report consolidates the tasks undertaken and accomplished as deliverables for the project. The project team had successfully completed all required tasks of phase 0a, confirming that the project is feasible based on the preliminary conceptual designs and operational requirements of the DAC systems. Of particular interest, the hub ownership structure was identified and was implemented in follow on projects that would transition the hub infrastructure to a build/operate phase.

36 MATERIALS SCIENCE

Use of Grid-Forming Medium-Voltage Power Electronics Hub in a Microgrid Setting

This paper presents the application of a new design of a multiport, modular, medium-voltage power electronics hub (M3PE-HUB) in a microgrid setting. The M3PE-HUB system was modeled in a digital real-time simulator (DRTS) and integrated into the Banshee microgrid test system. This paper presents the preliminary DRTS simulation-based results of the M3PE-HUB system connected in a test microgrid system. Verification and validation of the M3PE-HUB architecture and controls in the test microgrid setting are the primary contributions of this work. The results of the operation during the islanding and resynchronization process indicate the feasibility of the proposed architecture in a microgrid setting. This paper also presents results for a system reconfiguration use case where the M3PE-HUB was used to reconfigure the system under a fault condition.

grid forming

Computed Tomography Scanning and Geophysical Measurements of the Integrated Mid-Continent Stacked Carbon Storage Hub Sleepy Hollow Reagan Unit 86A Well

The Computed Tomography (CT) facilities, the Multi-Sensor Core Logger (MSCL), and the Geologic Storage Core Flow laboratory at the National Energy Technology Laboratory (NETL) in Morgantown, West Virginia and Pittsburgh, Pennsylvania were used to characterize a core through Upper Pennsylvanian strata (limestones, mudstones, and sandstones) from the Sleepy Hollow Reagan Unit (SHRU) 86A well in southwest-central Nebraska. The Integrated Mid-Continent Stack Carbon Storage Hub (IMSCS-HUB) core from the vertical well was obtained as part of the Department of Energy’s (DOE) effort to assess the feasibility of stacked storage complexes in Nebraska and Kansas to support a commercial-scale CO2 storage hub. The Sleepy Hollow Field (SHF) is one of three sites within the IMSCS-HUB corridor. Bulk scans of core were obtained from the IMSCS-HUB SHRU 86A well. This report, and the associated scans, include detailed datasets not typically made available to the public. The data sets presented in this report can be accessed from NETL's Energy Data eXchange (EDX) online system.

58 GEOSCIENCES

High-Power Electric Vehicle Charging Hub Integration Platform (eCHIP): Site Energy Management System Platform Development

A site energy management system is a critical component of future high-power EV charging hubs. It monitors and supervises all inter-hub operations, coordinates grid interactions, and implements energy management strategies and load-sharing controls at various levels and time scales. This report provides a comprehensive high-level overview and guidelines on the essential elements of such energy management systems. These elements primarily focus on the core functions, architecture, and monitoring aspects of site energy management. The core functions define the grid services and operational objectives that a charging hub is expected to provide and achieve. The architectural section investigates the coordination between hub assets, different control schemes, and their potential benefits and drawbacks. Lastly, the report discusses the needs, types, and functionalities of a monitoring and visualization system.

24 POWER TRANSMISSION AND DISTRIBUTION

DuraMAT Data Hub

The DuraMAT Data Hub has been supporting the consortium for the past six years. The Data Hub has had success in supporting the projects, providing a platform for sharing data within projects and to the public, and learning how to better leverage the existing software platform and the available Amazon Web Services environment. During this new generation of the Data Hub, we are looking at ways to help improve the data hub architecture, user experience, and improve operations by taking advantage of new technology platforms and software that will be more impactful on the consortium researchers and the broader scientific community. In this poster we will look at the current operational capabilities, data dissemination, and development that will improve the system in the near and far future.

14 SOLAR ENERGY

Charging Hub Scenario Sheet Screenshot

The Excel-based CHECT tool estimates the LCOC ($/kWh) by charger type (Level 1, Level 2, and DC fast charging) for a given charging hub scenario. CHECT requires users to input certain charging hub scenario parameters, including the number of chargers, daily utilization, and charger replacement frequency by charger type. Additional inputs such as the charging schedule, local utility rates, capital/operational costs, and financial inputs can be customized by the user, or the tool can generate results using appropriate default values from literature for the charging hub scenario and service location(s). Using the above inputs, CHECT performs a robust techno-economic analysis to generate the LCOC by charger type, broken down by cost category (e.g., capital, operational, utility, taxes) for various combinations of charging hub types (multiunit dwelling or public) and ownership models (residential, utility, or private company). It also outputs the annual discounted cash flows and determines the most sensitive input variables. In addition, the tool allows users to easily compare the LCOC across various ownership models or across different states.

32 ENERGY CONSERVATION, CONSUMPTION, AND UTILIZATI

Roughrider Carbon Storage Hub (Final Report)

The Roughrider Carbon Storage Hub was a 2-year project (October 2023 – September 2025) conducted by the Energy & Environmental Research Center (EERC) focused on advancing the feasibility of a commercial-scale carbon dioxide (CO 2 ) geologic storage hub in McKenzie County, North Dakota. The project’s objective was to investigate the potential that stacked storage complexes (multiple deep saline formations) can safely and economically store at least 50 million tonnes of CO 2 within 30 years. The captured CO 2 would be sourced from industrial emitters including project partner ONEOK, Inc.’s gas-processing plants and a planned gas-to-liquids facility. Drilling of the Roughrider 1 stratigraphic test well (14,979-ft total depth) was completed in November 2024. The wellbore intersected four candidate storage formations: Inyan Kara, Broom Creek, Mission Canyon, and Black Island–Deadwood. Operational challenges, including a stuck drill string, were resolved without long-term impact. A comprehensive logging and coring program was conducted, followed by successful well abandonment and site reclamation. Over 660 ft of 4-in. whole core was retrieved. Core plug samples were processed and analyzed for petrophysical and geochemical properties. Results confirmed promising porosity and permeability in the Inyan Kara and Broom Creek Formations and removal of the Mission Canyon and Black Island–Deadwood horizons from further investigation. Data derived from the logging and coring program were used to improve initial geologic models built from legacy data. CO 2 injection simulations showed that the Inyan Kara alone can feasibly store the target mass of CO 2 . Because of subtle differences in geologic structure and porosity trends between the formations, a stacked storage scenario using the Broom Creek and Inyan Kara Formations resulted in a larger overall plume area than using the Inyan Kara alone. Preliminary CO 2 pipeline routes from the industrial sources were mapped utilizing existing rights of way and evaluated for capacity and cost using U.S. Department of Energy Office of Fossil Energy and Carbon Management/National Energy Technology Laboratory models and U.S. Environmental Protection Agency emissions data. Integrating capture, transport, and storage cost estimates with policy incentives (e.g., 45Q credits) provided a total cost-per-ton analysis. Results indicate that the small scale of the volumes to be transported over the cumulative large distances does not support the project’s financial viability. However, the groundwork laid during this project from geological, regulatory, and social perspectives positions the Roughrider hub site as a promising candidate for commercial carbon storage in North Dakota, especially if the economy of scale is introduced for CO 2 transportation to the hub site.

01 COAL, LIGNITE, AND PEAT

Colorado (Pueblo) Regional DAC Hub TA-1: Feasibility (Phase 0a) (Final Technical Report)

This project supports the U.S. Department of Energy's (DOE) mission to reduce the environmental and climate impacts of fossil fuels and industrial processes, contributing to the goal of achieving net-zero emissions across the U.S. economy. The primary objective is to conduct a feasibility study for a Regional Direct Air Capture (DAC) Hub in the Southern Colorado region, northeast of Pueblo. The geographic construct of this hub is based on the Denver-Julesburg Basin – a geological area where a significant number of geological storage studies have been conducted (See Figure 1). The project will leverage the work of Project Eos, a CarbonSAFE Phase III study led by the Colorado School of Mines and CarbonAmerica. The DAC Hub aims to capture, store, and/or utilize at least 1,000,000 tonnes of CO 2 from the atmosphere annually. To achieve this, the project team is designing a system with an initial capacity of 100,000 tonnes per year. This feasibility-stage project will formulate the Regional DAC Hub concept and team to conduct the relevant analysis, networking and community stakeholder engagement necessary to advance the project to the design stage.

42 ENGINEERING

Building a Trusted Roaming Hub [Slides]

The Trusted Roaming Hub is a U.S. Department of Energy-backed initiative led by the National Laboratory of the Rockies (NLR) to address one of the most persistent challenges in electric vehicle (EV) charging: fragmented roaming, inconsistent interoperability, and insufficient digital trust across charging networks. As EV adoption accelerates and charging infrastructure scales nationwide, today's many-to-many integration model between eMobility Service Providers (eMSPs) and Charge Point Operators (CPOs) has become increasingly brittle, costly, and difficult to secure. The Trusted Roaming Hub introduces a neutral, cybersecurity-forward "switchboard" architecture that enables standardized, secure, and scalable roaming interactions across the EV charging ecosystem. Rather than replacing existing networks or commercial relationships, the hub acts as a trusted intermediary that enforces consistent identity, authentication, authorization, and routing across participants improving reliability for drivers, lowering integration burden for industry, and creating a foundation for future grid-interactive charging services. This read-ahead provides an overview of the problem the hub is designed to solve, the core functional and security concepts behind the architecture, the value proposition to key stakeholders, and the near-term trajectory of the work.

33 ADVANCED PROPULSION SYSTEMS

Exploring the Feasibility of a Carbon Dioxide Storage Hub in Western North Dakota

The University of North Dakota Energy & Environmental Research Center (EERC) and project partner ONEOK, Inc. (ONEOK) are investigating the feasibility of establishing a CO2 (carbon dioxide) storage hub in western North Dakota—in the heart of the Williston Basin. The conceived Roughrider Carbon Storage Hub would store CO2 captured from six gas-processing plants owned and operated by project partner ONEOK and a planned gas-to-liquids plant. This 2-year U.S. Department of Energy-sponsored Carbon Storage Assurance Facility Enterprise (CarbonSAFE) Phase II feasibility study is evaluating the aggregation of the CO2 captured from these seven sources for injection into stacked geologic storage complexes. The proposed hub includes several aspects that make it a highly qualified candidate for a feasibility study with a notably reduced project risk profile. These include 1) a project partner (ONEOK) with a committed goal to reduce greenhouse gas emissions; 2) prior subsurface data analysis supporting a potential stacked storage configuration with adequate CO2 storage resource; 3) commitment from local, regional, and state-level stakeholders; and 4) a state with U.S. Environmental Protection Agency underground injection control Class VI primacy. ONEOK’s assets in the Williston Basin provide significant environmental benefits by capturing and processing natural gas that may otherwise be flared or vented. Storing CO2 from these gas-processing facilities will reduce overall CO2 emissions in the basin while providing essential services to producers there and contributing to continued energy independence in the domestic markets.

03 NATURAL GAS

Hydrogen Hub Systems Analysis and Mapping Tool (ParaCraft) v1

A plug and play techno-economic analysis (TEA) and lifecycle assessment (LCA) tool was built that could incorporate new projects into the California ARCHES LLC Hydrogen hub, and generate results for the project, as well as the overall hub on an annual basis. The model was first constructed in Microsoft Excel and ArcGIS, but required labor intensive updating and manual decision making regarding the matching of hydrogen supplier and offtaker and estimation of transportation distances and utility sources. The project team converted the Excel model used for the ARCHES LLC hub conceptualization into a highly flexible and nearly completely automated R code. The R code runs the TEA and LCA, as well as provides mapping capabilities that automatically link projects by latitude and longitude to nearby utilities.

Breunig, Hanna

Pre-FEED Report on the Tampa Regional Intermodal Carbon Hub (T-RICH)

This report presents the findings of a year-long pre-FEED study into the feasibility of a CO 2 intermodal hub in Port Tampa Bay which would collect, store, and liquify captured CO 2 from major emitters across the State of Florida for marine transport out of the state. The proposed hub is called the Tampa Regional Intermodal Carbon Hub (T-RICH).

99 GENERAL AND MISCELLANEOUS

Capacitated p -hub approach for park-and-ride facility location problem under nested logit demand function: polyhedral approaches

By generalizing the unconstrained p-hub approach for the park-and-ride (P&R) facility location problem under the multinomial logit demand function, the capacitated p-hub approach for the problem under the nested logit demand function captures a broader range of real-world cases. To solve this problem optimally, we introduce a mixed-integer linear program and accelerate its solution by enhancing the branch-and-cut procedure. To address the problem at a large scale, we introduce two other polyhedral approaches: variable neighborhood search (VNS) and adaptive randomized rounding (ARR). Downtown areas in Seoul have a high modal share of public transportation and congested road traffic, yet P&R has not been widely implemented. Therefore, we apply the ARR procedure to solve a real-world problem using traffic and geographic data from the Seoul metropolitan area. ARR performs better than VNS and addresses real-world cases. The solutions obtained by ARR present a phased expansion plan that encourages policymakers to start installing a small number of P&Rs immediately.

Capacitated p-hub approach

Florida Regional DAC Hub

This project supported the U.S. Department of Energy's (DOE) mission to reduce the environmental and climate impacts of fossil fuels and industrial processes, contributing to the goal of achieving net-zero emissions across the U.S. economy. The primary objective is to conduct a feasibility study for a Regional Direct Air Capture (DAC) Hub in Bay County, Florida. This hub takes advantage of the region’s deep, permeable saline aquifers (Tuscaloosa Group, 1,500–2,150 meters deep), which have been the focus of numerous geological storage studies.

42 ENGINEERING

Energy Storage Impacts in Resilience Hubs and Other Critical Infrastructure: An Assessment Guide for Developers and Practitioners

Battery energy storage systems (BESS) deployed behind the meter at resilience hubs and other critical infrastructure can provide economic and operational value during normal operations—such as lower and more predictable energy costs—as well as resilience and security benefits during power disruptions by maintaining essential services. However, existing evaluation approaches tend to focus narrowly on engineering performance or rely on broad socio-economic frameworks that are not well suited to behind-the-meter storage. As a result, developers, utilities, and funders often lack consistent methods for defining success, quantifying benefits, and comparing outcomes across projects. This report presents a practitioner-oriented impact assessment framework for evaluating behind-the-meter BESS at resilience hubs and critical infrastructure facilities. The framework is organized into five iterative components—developing an action plan, defining project goals, identifying metrics, collecting data and measuring outcomes, and reporting and using results—and includes a structured metric architecture spanning six impact categories. Designed for real-world constraints such as limited staffing and uneven data availability, the framework was developed, applied, and refined through real projects, and is illustrated with case studies across diverse deployment contexts.

Impact Assessment