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At least 19 records

Two-mirrored galvanometer laser light sheet generator

Light sheets generated with either laser or noncoherent sources have found widespread application to flow visualization. Previous light sheet generating systems were usually dedicated to a specific viewing geometry. The technique with the most flexibility is the galvanometer mirror based laser light sheet system. A two-mirrored system was designed and developed to provide flexibility and adaptability to a wide range of applications. The design includes the capability to control the size and location of the laser light sheet in real time, to generate horizontal or vertical sheets, to sweep the sheet repeatedly through a volume, to generate multiple sheets with controllable separation and to rotate single or multiple laser light sheets. The system is capable of producing up to 12 sheets of laser light at an angular divergence of + or - 20 degrees. Maximum scan rate of any one line is 500 Hertz. This system has proven to be uniquely versatile and a patent has been applied for.

Leighty, B. D.

Partial Spreading of a Laser Beam into a Light Sheet by Shock Waves and Its Use as a Shock Detection Technique

It is observed that when a laser beam is allowed to fall on a shock surface at a grazing incidence, a small part of the beam spreads out in a thin, diverging sheet of light normal to the surface, and both upstream and downstream of the shock. The phenomenon is visualized by observing a cross section of the light sheet on a screen placed normal to the laser path after it touches a shock. The light sheet disappears when the beam is moved to any other locations where there is no shock or the beam pierces the shock surface, i.e., at a non-grazing incidence. The spread angle of the light sheet is considerably higher than the angle by which the beam may bend as it passes through the shock, which produces a small difference of refractive index. Various details indicate that the spread light is a result of diffraction of a small part of the laser beam by the shock whose thickness is nearly the same as that of the laser wavelength. Shocks formed in underexpanded free jets of fully expanded Mach numbers 1.4 to 1.8 are used for this experiment. The above optical phenomenon is used as the basis of a novel shock detection technique which depends on sensing the spread light using a photomultiplier tube (PMT). The locations of the shock surfaces in the underexpanded supersonic jet, obtained using this technique, match with those inferred from the Schlieren photographs and velocity measurements. Moreover, if the shock oscillates, a periodic PMT signal is obtained which provides information about the frequency and amplitude of shock motion.

Panda, J.

Image processing tools for petabyte-scale light sheet microscopy data

Light sheet microscopy is a powerful technique for high-speed three-dimensional imaging of subcellular dynamics and large biological specimens. However, it often generates datasets ranging from hundreds of gigabytes to petabytes in size for a single experiment. Conventional computational tools process such images far slower than the time to acquire them and often fail outright due to memory limitations. To address these challenges, we present PetaKit5D, a scalable software solution for efficient petabyte-scale light sheet image processing. This software incorporates a suite of commonly used processing tools that are optimized for memory and performance. Notable advancements include rapid image readers and writers, fast and memory-efficient geometric transformations, high-performance Richardson–Lucy deconvolution and scalable Zarr-based stitching. These features outperform state-of-the-art methods by over one order of magnitude, enabling the processing of petabyte-scale image data at the full teravoxel rates of modern imaging cameras. The software opens new avenues for biological discoveries through large-scale imaging experiments.

97 MATHEMATICS AND COMPUTING

Study of optical techniques for the Ames unitary wind tunnels. Part 2: Light sheet and vapor screen

Light sheet and vapor screen methods have been studied with particular emphasis on those systems that have been used in large transonic and supersonic wind tunnels. The various fluids and solids used as tracers or light scatters and the methods for tracing generation have been studied. Light sources from high intensity lamps and various lasers have been surveyed. Light sheet generation and projection methods were considered. Detectors and location of detectors were briefly studied. A vapor screen system and a technique for location injection of tracers for the NASA Ames 9 by 7 foot Supersonic Wind Tunnel were proposed.

Lee, George

A twin-mirrored galvanometer laser light sheet generator

A galvanometer mirror-based laser light sheet system has been developed for use in the Basic Aerodynamics Research Tunnel at NASA Langley. This system generates and positions single or multiple light sheets over aeronautical research models being tested in the low speed tunnel. This report describes a twin mirrored galvanometer laser light sheet generator and shows typical light sheet arrangements in use. With this system, illumination of smoke entrained in the flow over a delta wing model reveals the vortical flow produced by the separation of the flow at the leading edge of the model. The light sheet system has proven to be very adaptable and easy to use in sizing and positioning light sheets in wind tunnel applications.

Rhodes, David B.

Twin-Mirrored-Galvanometer Laser-Light-Sheet Generator

Multiple, rotating laser-light sheets generated to illuminate flows in wind tunnels. Designed and developed to provide flexibility and adaptability to wide range of applications. Design includes capability to control size and location of laser-light sheet in real time, to generate horizontal or vertical sheets, to sweep sheet repeatedly through volume, to generate multiple sheets with controllable separation, and to rotate single or multiple laser-light sheets. Includes electronic equipment and laser mounted on adjustable-height platform. Twin-mirrored galvanometer unit supported by tripod to reduce vibration. Other possible applications include use in construction industry to align beams of building. Artistic or display applications also possible.

Rhodes, David B.

Scalable Thin Light-Emitting Diode (LED) Light Sheet Platform

The goal of the Scalable Thin Light-Emitting Diode (LED) Light Sheet Platform project is to derisk the manufacturing scalability for a high chip count heterogeneous smart lighting platform, with the potential to enable significant energy savings through dynamic directional light control and custom. Specifically, the project derisks the scalability of a disruptive new computer controlled microassembly fabrication process SRI International is developing, to address fundamental cost barriers to mass production of high chip count systems. This is key for enabling mass adoption and thus maximum societal energy savings impact. The LED light sheet technology is a smart illumination platform that can be applied to lighting, signage, and display. It has features and a form factor similar to bendable or conformal OLED light sheets but uses more efficient LEDs with a remote phosphor layer that is very close to the LED to deliver a luminous efficacy that exceeds 125 lumen/Watt and enable > 50% Lighting Application Efficiency (LAE) energy savings. During Budget Period 1 (BP1), all of the BP1 milestones were successfully completed: (M2.0.1) Design demonstrator details that will meet the final project goals, (M6.1.1) Show lighting output model can predict illuminance, spatial, spectral distribution for specific light-sheet designs and use case, (M3.2.1) Automated loading supports 200 chip arrays, and (M3.3.1) Finish first interconnect process run for 200 chip array. The BP1 Go/No-Go Decision Point (G/NG 1) 200 chip demonstrator sample was in the process of final assembly and test at the end of BP1 on December 31, 2023. The objective was successfully achieved on January 16, 2024, when a wired 200-chip sample underwent confirmation testing that demonstrated a 93.75% first pass electrical yield that successfully exceeded the 75% first-pass electrical yield BP1 G/NG requirement. In addition, all 93.75% of the sample devices lit up, further demonstrating the ability to assemble and transfer small LED chips without damaging their functionality.

32 ENERGY CONSERVATION, CONSUMPTION, AND UTILIZATI

Computer-aided light sheet flow visualization

A computer-aided flow visualization process has been developed to analyze video images acquired from rotating and translating light sheet visualization systems. The computer process integrates a mathematical model for image reconstruction, advanced computer graphics concepts, and digital image processing to provide a quantitative and visual analysis capability. The image reconstruction model, based on photogrammetry, uses knowledge of the camera and light sheet locations and orientations to project two-dimensional light sheet video images into three-dimensional space. A sophisticated computer visualization package, commonly used to analyze computational fluid dynamics (CFD) data sets, was chosen to interactively display the reconstructed light sheet images, along with the numerical surface geometry for the model or aircraft under study. A description is provided of the photogrammetric reconstruction technique, and the image processing and computer graphics techniques and equipment. Results of the computer aided process applied to both a wind tunnel translating light sheet experiment and an in-flight rotating light sheet experiment are presented. The capability to compare reconstructed experimental light sheet images and CFD solutions in the same graphics environment is also demonstrated.

Stacy, Kathryn

Computer-Aided Light Sheet Flow Visualization

A computer-aided flow visualization process has been developed to analyze video images acquired from rotating and translating light sheet visualization systems. The computer process integrates a mathematical model for image reconstruction, advanced computer graphics concepts, and digital image processing to provide a quantitative and visual analysis capability. The image reconstruction model, based on photogrammetry, uses knowledge of the camera and light sheet locations and orientations to project two-dimensional light sheet video images into three-dimensional space. A sophisticated computer visualization package, commonly used to analyze computational fluid dynamics (CFD) data sets, was chosen to interactively display the reconstructed light sheet images, along with the numerical surface geometry for the model or aircraft under study. A description is provided of the photogrammetric reconstruction technique, and the image processing and computer graphics techniques and equipment. Results of the computer aided process applied to both a wind tunnel translating light sheet experiment and an in-flight rotating light sheet experiment are presented. The capability to compare reconstructed experimental light sheet images and CFD solutions in the same graphics environment is also demonstrated.

Stacy, Kathryn

Computer-aided light sheet flow visualization using photogrammetry

A computer-aided flow visualization process has been developed to analyze video images acquired from rotating and translating light sheet visualization systems. The computer process integrates a mathematical model for image reconstruction, advanced computer graphics concepts, and digital image processing to provide a quantitative and a visual analysis capability. The image reconstruction model, based on photogrammetry, uses knowledge of the camera and light sheet locations and orientations to project two-dimensional light sheet video images into three-dimensional space. A sophisticated computer visualization package, commonly used to analyze computational fluid dynamics (CFD) results, was chosen to interactively display the reconstructed light sheet images with the numerical surface geometry for the model or aircraft under study. The photogrammetric reconstruction technique and the image processing and computer graphics techniques and equipment are described. Results of the computer-aided process applied to both a wind tunnel translating light sheet experiment and an in-flight rotating light sheet experiment are presented. The capability to compare reconstructed experimental light sheet images with CFD solutions in the same graphics environment is also demonstrated.

Stacy, Kathryn

Generating A Strobed Laser Light Sheet

An optoelectronic system generating synchronous, strobed sheet of laser light developed for use in making visible flow of air about model helicopter rotor. Used in wind-tunnel tests to determine actual locations of vortices for comparison with locations predicted by mathematical models to validate models. Each blade tip produces vortex. By establishing successive vortex locations, researcher determines trajectory of vortex pattern. Light-sheet strobe circuits provide selection of blade positions, strobe-pulse durations, and multiple pulses per revolution for rotors having two to nine blades. To make flow visible, vaporizing propylene glycol injected upstream of model. System also provides calibrated trigger delay of strobe pulses, adjustable strobe-pulse durations, selectable number of blades, and slip-sync mode to make flow visible as though in slow motion.

Leighty, Bradley D.

Single-Objective Airy Light-Sheet Imaging

Despite its massive potential, standard light-sheet imaging (LSI) faces key challenges, such as the incompatibility with common sample mounting techniques and low-resolution imaging. Single-objective LSI attempts to address these issues but often suffers from limited fields-of-view and throughput rates, or requires multiple optics that increase costs, alignment complexity, and losses. To overcome these challenges of standard single-objective LSI, we introduce single-objective Airy light-sheet imaging (SoALSI). SoALSI leverages the extraordinary self-acceleration properties of the Airy beam, achieving 5× higher imaging rates and enhanced imaging efficiency than standard single-objective LSI. Here, we demonstrate SoALSI’s versatility through rigorous contrast and resolution characterizations and by high-resolution imaging of diverse biological specimens, including malaria parasite-infected red blood cells and plant root tissue. SoALSI seamlessly integrates with any standard inverted microscope frame, enabling broader accessibility for the bioimaging community to explore biological processes in a wide range of specimens with enhanced resolution and imaging contrast.

airy beam

A synchronous strobed laser light sheet for helicopter model rotor flow visualization

A synchronous, strobed laser light sheet has been developed for use in flow visualization of a helicopter rotor model. The light sheet strobe circuit included selectable blade position, strobe duration, and multiple pulses per revolution for rotors having 2 to 9 blades. The flow was seeded with propylene glycol. Between runs, a calibration grid board was placed in the plane of the laser sheet and recorded with the video camera at the position used to record the flow field. A slip-sync mode permitted slow motion visualization of the flow field over complete rotations of the rotor. The system was used to make two-dimensional flow field cuts of a four-bladed rotor operating at advance ratio of 0.37 at wind tunnel speeds up to 79.25 meters per second (260 feet per second).

Leighty, Bradley D.

A preliminary study on ice shape tracing with a laser light sheet

Preliminary work towards the development of an automated method of measuring the shape of ice forming on an airfoil during wind tunnel tests has been completed. A thin sheet of light illuminated the front surfaces of rime, glaze, and mixed ice shapes and a solid-state camera recorded images of each. A maximum intensity algorithm extracted the profiles of the ice shapes and the results were compared to hand tracings. Very good general agreement was found in each case.

Mercer, Carolyn R.

Fast Light-Sheet Scanner

Optomechanical apparatus maintains sheet of pulsed laser light perpendicular to reference axis while causing sheet of light to translate in oscillatory fashion along reference axis. Produces illumination for laser velocimeter in which submicrometer particles entrained in flow illuminated and imaged in parallel planes displaced from each other in rapid succession. Selected frequency of oscillation range upward from tens of hertz. Rotating window continuously shifts sheet of light laterally while maintaining sheet parallel to same plane.

Hunter, William W., Jr.

Lattice light-sheet microscopy allows for super-resolution imaging of receptors in leaf tissue

Plant leaf tissues are difficult to image via fluorescent microscopy, largely due to the presence of chlorophyll and other pigments that provide large background fluorescence. An advantage of Lattice Light-Sheet microscopy is its use of Bessel beams that illuminate a thin focal region of interest for microscopy, allowing for the excitation of fluorescent molecules within this region without surrounding chlorophyll-like objects outside of the region of interest. Here, we apply STORM Super-resolution techniques to observe Receptor-Like Kinases in Arabidopsis thaliana leaf cells. By applying this technique with the Lattice Light-Sheet, we can localize immune response proteins in sub-100 nm length scales and reconstruct three-dimensional locations of proteins within individual leaf cells. Using this technique, we observed the effect of the elicitors ATP and flg22, where we observed a significant degree of internalization of cognate receptors P2K1 and FLS2. We were also able to similarly observe differences in colocalization due to stimulation with these elicitors, where we observe proteins on the membrane becoming less colocalized as a result of stimulation, suggesting an immune response mechanism involving receptors internalizing via pathways distinct to the receptor. Further, these data show the Lattice Light-Sheet’s capabilities for imaging tissue with problematic background fluorescence that otherwise makes super-resolution fluorescence microscopy difficult.

59 BASIC BIOLOGICAL SCIENCES

Scanning Light Sheet Would Measure Deflection Of Beam

Scanning-light-sheet apparatus designed to measure linear and angular displacement or deflection of structure. Intended specifically to measure deflection of beam-shaped truss structure. Includes conventional low-powered laser, lenses, mounts, single-axis optical scanner, several photodiodes, and electronic controller. Apparatus measures motion of structure and also used to determine positions, deflections, and velocities. Besides use in aerospace field, displacement measurements have many applications in construction-equipment and automotive industries.

Tcheng, Ping