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At least 19 records

Reverse osmosis filtration for space mission wastewater: membrane properties and operating conditions

Reverse osmosis (RO) is a compact process that has potential for the removal of ionic and organic pollutants for recycling space mission wastewater. Seven candidate RO membranes were compared using a batch stirred cell to determine the membrane flux and the solute rejection for synthetic space mission wastewaters. Even though the urea molecule is larger than ions such as Na+, Cl-, and NH4+, the rejection of urea is lower. This indicates that the chemical interaction between solutes and the membrane is more important than the size exclusion effect. Low pressure reverse osmosis (LPRO) membranes appear to be most desirable because of their high permeate flux and rejection. Solute rejection is dependent on the shear rate, indicating the importance of concentration polarization. A simple transport model based on the solution-diffusion model incorporating concentration polarization is used to interpret the experimental results and predict rejection over a range of operating conditions. Grant numbers: NAG 9-1053.

Non-NASA Center

Membrane has high urea-rejection properties

Membranes are synthesized from ethylene and nitrogen in RF plasma at low power, gas-flow rates, and pressure. Ethylene and nitrogen are used because flow rate and partial pressure of each gas can be independently controlled to produce optimum conditions for synthesizing membrane. Membrane is particularly useful in recycling and purifying water.

Johnson, C. C.

Amphiphilic components of the Murchison carbonaceous chondrite - Surface properties and membrane formation

The possibility that the amphiphilic compounds in carbonaceous meteorites whose physicochemical properties are presently studied may represent sources of lipidlike compounds which could have evolved as membrane components in primitive cells is investigated in samples of the Murchison CM2 chondrite. Surface properties and membrane formation are obtained for three fractions isolated by two-dimensional thin-layer chromatography. It is concluded that a small, undefined fraction of Murchison components exhibits amphiphilic properties which allow assembly into boundary membranes.

Deamer, D. W.

Characterizing Shear Properties of Membranes

Method devised to obtain static shear stiffness data for membranes. Shear deflection of membrane measured under various applied static shear loads, and measurements plotted. Match attempted between experimental plot and predicted load-vs.-deflections curves until best fitting theoretical curve is found.

Simpson, B.

Reverse osmosis membrane of high urea rejection properties

Polymeric membranes suitable for use in reverse osmosis water purification because of their high urea and salt rejection properties are prepared by generating a plasma of an unsaturated hydrocarbon monomer and nitrogen gas from an electrical source. A polymeric membrane is formed by depositing a polymer of the unsaturated monomer from the plasma onto a substrate, so that nitrogen from the nitrogen gas is incorporated within the polymer in a chemically combined form.

Johnson, C. C.

The delayed rectifier, IKI, is the major conductance in type I vestibular hair cells across vestibular end organs

Hair cells were dissociated from the semicircular canal, utricle, lagena and saccule of white king pigeons. Type I hair cells were identified morphologically based on the ratios of neck width to cuticular plate width (NPR < 0.72) as well as neck width to cell body width (NBR < 0.64). The perforated patch variant of the whole-cell recording technique was used to measure electrical properties from type I hair cells. In voltage-clamp, the membrane properties of all identified type I cells were dominated by a predominantly outward potassium current, previously characterized in semicircular canal as IKI. Zero-current potential, activation, deactivation, slope conductance, pharmacologic and steady-state properties of the complex currents were not statistically different between type I hair cells of different vestibular end organs. The voltage dependence causes a significant proportion of this conductance to be active about the cell's zero-current potential. The first report of the whole-cell activation kinetics of the conductance is presented, showing a voltage dependence that could be best fit by an equation for a single exponential. Results presented here are the first data from pigeon dissociated type I hair cells from utricle, saccule and lagena suggesting that the basolateral conductances of a morphologically identified population of type I hair cells are conserved between functionally different vestibular end organs; the major conductance being a delayed rectifier characterized previously in semicircular canal hair cells as IKI.

NASA Discipline Number 40-99

Anion permselective membrane

The objective of NAS 3-20108 was the development and evaluation of improved anion selective membranes useful as efficient separators in a redox power storage cell system being constructed. The program was divided into three parts, (a) optimization of the selected candidate membrane systems, (b) investigation of alternative membrane/polymer systems, and (c) characterization of candidate membranes. The major synthesis effort was aimed at improving and optimizing as far as possible each candidate system with respect to three critical membrane properties essential for good redox cell performance. Substantial improvements were made in 5 candidate membrane systems. The critical synthesis variables of cross-link density, monomer ratio, and solvent composition were examined over a wide range. In addition, eight alternative polymer systems were investigated, two of which attained candidate status. Three other alternatives showed potential but required further research and development. Each candidate system was optimized for selectivity.

Alexander, S.

Atmospheric Electron-induced X-Ray Spectrometer (AEXS) Instrument Development

Instrument Development: The membrane properties were investigated in detail due to its critical role. A series of experiments determined that our designed membranes have high electron transmission, yet are capable of isolating high vacuum, are able to withstand differential pressure in excess of one atmosphere, survive vibrational shocks of a magnitude to be expected during a planetary mission. Our initial work has been with a 10 keV source and a 200 nm thick Silicon Nitride (SiN) encapsulation membrane micro-fabricated within a Si support frame (1.5 mm x 1.5 mm window openings. Fig 2 compares the spectra taken with the 10 keV source with spectra taken within SEM. Introduction: This paper describes the progress in the development of the AEXS instrument in our laboratory at JPL. The AEXS is a novel miniature instrument[1-3] based on the excitation of characteristic X-Ray Fluorescence (XRF) and luminescence spectra using a focused electron beam, for non-destructive evaluation of surfaces of samples in planetary ambient atmosphere. In situ operation is obtained through the use of a thin electron transmissive membrane to isolate the vacuum within the AEXS electron source from the outside ambient atmosphere. The impinging electrons excite XRF spectra from the irradiated spots on samples in external atmosphere with high-to-medium (sub-mm to cm-scale) spatial resolution at Mars atmospheric pressure. The XRF spectra are analyzed using an energy-dispersive detector to determine surface elemental composition, or in the case of electron-induced luminescence to identify unusual formations on surface that cathodo-luminescence (CL). The AEXS system (Fig 1) consists of a high-energy (>10keV) electron gun encapsulated by the isolation membrane, an EDX detection and analyzer system to determine the elemental abundance, an optional CL detection system, and a high voltage power supply. The approach to demonstrating a proof of concept of the AEXS has been through 1) demonstrating the viability of micro-fabricated membranes, 2) assembling AEXS setups with increasingly integrated functional components, and 3) simulating the AEXS observational capabilities. This paper will focus on description of the development of the instrument.

Wilcox, J. Z.

A laser microsurgical method of cell wall removal allows detection of large-conductance ion channels in the guard cell plasma membrane

Application of patch clamp techniques to higher-plant cells has been subject to the limitation that the requisite contact of the patch electrode with the cell membrane necessitates prior enzymatic removal of the plant cell wall. Because the wall is an integral component of plant cells, and because cell-wall-degrading enzymes can disrupt membrane properties, such enzymatic treatments may alter ion channel behavior. We compared ion channel activity in enzymatically isolated protoplasts of Vicia faba guard cells with that found in membranes exposed by a laser microsurgical technique in which only a tiny portion of the cell wall is removed while the rest of the cell remains intact within its tissue environment. "Laser-assisted" patch clamping reveals a new category of high-conductance (130 to 361 pS) ion channels not previously reported in patch clamp studies on plant plasma membranes. These data indicate that ion channels are present in plant membranes that are not detected by conventional patch clamp techniques involving the production of individual plant protoplasts isolated from their tissue environment by enzymatic digestion of the cell wall. Given the large conductances of the channels revealed by laser-assisted patch clamping, we hypothesize that these channels play a significant role in the regulation of ion content and electrical signalling in guard cells.

Non-NASA Center

Aqueous two-phase partition applied to the isolation of plasma membranes and Golgi apparatus from cultured mammalian cells

Partitioning in dextran-poly(ethylene)glycol (PEG) aqueous-aqueous phase systems represents a mature technology with many applications to separations of cells and to the preparation of membranes from mammalian cells. Most applications to membrane isolation and purification have focused on plasma membranes, plasma membrane domains and separation of right side-out and inside-out plasma membrane vesicles. The method exploits a combination of membrane properties, including charge and hydrophobicity. Purification is based upon differential distributions of the constituents in a sample between the two principal compartments of the two phases (upper and lower) and at the interface. The order of affinity of animal cell membranes for the upper phase is: endoplasmic reticulum <mitochondria<Golgi apparatus<lysosomes and endosomes< plasma membranes. Salt concentrations and temperature affect partitioning behavior and must be precisely standardized. In some cases, it is more fortuitous to combine aqueous two-phase partition with other procedures to obtain a more highly purified preparation. A procedure is described for preparation of Golgi apparatus from transformed mammalian cells that combines aqueous two-phase partition and centrifugation. Also described is a periodic NADH oxidase, a new enzyme marker for right side-out plasma membrane vesicles not requiring detergent disruptions for measurement of activity.

Non-NASA Center

A 4-node Bilinear Isoparametric Element in Rockwell NASTRAN

The development and evaluation of the Rockwell NASTRAN four node quadrilateral (QUAD4) element is presented. The element derivation utilizes bilinear isoparametric techniques both for membrane and bending characteristics. The QUAD4 element coordinate system, membrane properties, lumped mass matrix, and treatment of warping are based upon the COSMIC/NASTRAN QDMEMI element while the bending characteristics are based on a paper by T. J. R. Hughes. The effects of warping on the bending stiffness, consistent mass, and geometric stiffness are based upon a paper by R. H. MacNeal. Numerical integration is accomplished by Gaussian quadrature on a 2 x 2 grid. Practical user support features include variable element thickness, thermal analysis and layered composite material definitions.

Liao, C.

Cellular and Network Mechanisms Underlying Information Processing in a Simple Sensory System

Realistic, biophysically-based compartmental models were constructed of several primary sensory interneurons in the cricket cercal sensory system. A dynamic atlas of the afferent input to these cells was used to set spatio-temporal parameters for the simulated stimulus-dependent synaptic inputs. We examined the roles of dendritic morphology, passive membrane properties, and active conductances on the frequency tuning of the neurons. The sensitivity of narrow-band low pass interneurons could be explained entirely by the electronic structure of the dendritic arbors and the dynamic sensitivity of the SIZ. The dynamic characteristics of interneurons with higher frequency sensitivity required models with voltage-dependent dendritic conductances.

Jacobs, Gwen

SOLAR SAIL PROPULSION SENSITIVITY TO MEMBRANE SHAPE AND OPTICAL PROPERTIES USING THE SOLAR VECTORING EVALUATION TOOL (SVET)

Solar sail propulsive performance is dependent on sail membrane optical properties and on sail membrane shape. Assumptions of an ideal sail (flat, perfect reflector) can result in errors which can affect spacecraft control, trajectory analyses, and overall evaluation of solar sail performance. A MATLAB(R) program has been developed to generate sail shape point cloud files for two square-architecture solar sail designs. Simple parabolic profiles are assumed for sail shape under solar pressure loading. These files are then input into the Solar Vectoring Evaluation Tool (SVET) software to determine the propulsive force vector, center of pressure, and moments about the sail body axes as a function of sail shape and optical properties. Also, the impact of the center-line angle, due to non-perfect optical properties, is addressed since this constrains sail force vector cone angle and is often overlooked when assuming ideal-reflector membranes. Preliminary sensitivity analysis using these tools aids in determining the key geometric and optical parameters that drive solar sail propulsive performance.

Ewing, Anthony