Control of cell division by the electrical voltage of the surface membrane
Control of cell division by electrical voltage of surface membrane
Cone, C. D., Jr.↗
SEARCH · Search NASA
Results for “membrane surface”
Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.
Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.
Diffusion mediated localization on membrane surfaces
Using the model of a cell membrane of a spherical surface in which membrane components may diffuse, the rate of localization due to trapping under diffusion control has been estimated by computing an analytical expression for the mean trapping time including the possibilities of a trapping probability less than one and/or the establishment of an equilibrium at the trap boundary.
Weaver, D. L.↗
Peptides at Membrane Surfaces and their Role in Prebiotic Evolution
Protocells had to transport ions and organic matter across membranes separating the interior of the cell from the environment, capture and utilize energy and transduce environmental signals. In a series of detailed, molecular-level computer simulations we show how these peptides in contact with membranes can acquire ordered structures and functions. We have investigated the stability of a simple alpha-helical peptide containing Leucine (L) and Serine (S) of the form (LSLLLSL)3 in a model membrane system. The parallel in-plane state is the most stable configuration. The transmembrane state is metastable, and about 15 kcal/mol is required to insert the peptide into the membrane. We investigated dimes of both (LSLLLSL)3 and glycophorin A, and show how the free energy of helix association can, at least partially, offset the free energy of insertion. We have also investigated the transmembrane pore of the influenza M2 protein. This aggregate of four identical alpha-helices, each built of 25 amino acids, forms an efficient and selective voltage-gated proton channel. Our simulations explain the gating mechanism, which can involve strands of hydrogen-bonded water through the pore or proton transfer through tautomerization of protein residues. The channel can be re-engineered to act as a simple proton pump.
Pohorille, Andrew↗
Peptides at Membrane Surfaces and their Role in the Origin of Life
All ancestors of contemporary cells (protocells) had to transport ions and organic matter across membranous walls, capture and utilize energy and transduce environmental signals. In modern organisms, all these functions are preformed by membrane proteins. We make the parsimonious assumption that in the protobiological milieu the same functions were carried out by their simple analogs - peptides. This, however, required that simple peptides could self-organize into ordered, functional structures. In a series of detailed, molecular-level computer simulations we demonstrated how this is possible. One example is the peptide (LSLLLSL)3 which forms a trameric bundle capable of transporting protons across membranes. Another example is the transmembrane pore of the influenza M2 protein. This aggregate of four identical alpha-helices, each built of 25 amino acids, forms an efficient and selective voltage-gated proton channel. Our simulations explain the gating mechanism in this channel. The channel can be re-engineered into a simple proton pump.
Pohorille, Andrew↗
On minimal surfaces, membranes and compressible flows
The theory, variational formulation and finite element analysis of a class of nonlinear problems arising in optimization, thin membrane deflection and compressible flows are developed. Error estimates are summarized, and representative numerical results are presented. Particular theoretical questions pertaining to the existence and uniqueness of solutions are addressed, and their implications for such applications of finite element analysis as the study of compressible flows indicated.
Carey, G. F.↗
Shaping off-axis metallic membrane reflectors using optimal boundary shapes and inelastic strains
This paper will describe a novel concept for constructing off-axis membrane reflector surfaces. Membrane reflectors have been extensively studied, including investigations into inflated lenticular architectures, shaping by spin casting, shaping using electrostatic forces, and shaping by evacuating behind a membrane surface stretched between circular or annular-shaped supports.
membrane↗
Modeled Microgravity Inhibits Apoptosis in Peripheral Blood Lymphocytes
Impairment of the immunity in astronauts and cosmonauts even in short term flights is a recognized risk. Long term orbital space missions and anticipated interplanetary flights increase the concern for more pronounced effects on the immune system with potential clinical consequences. Impairment of the immunity in space may be due tonumerous physiological changes caused by space-related factors, which in turn affect the immune system, or alternatively, it may be due to direct effects of different factors encountered in space on lymphoid cells and their interactions. Indeed, in modeled microgravity (MMG) experiments on Earth we and others showed that microgravity directly affects multiple lymphocyte functions. It interferes with expression of cell surface molecules, causes inhibition of lymphocyte locomotion, suppresses polyclopal and antigen-specific lymphocyte activation, selectively inhibits protein kinase C (PKC) isoforms. Some of these effects were also confirmed in cell culture experiments in real space conditions during Spacelab, Biokosmos and Shuttle Missions. The results of these studies, taken together, strongly indicated that microgravity interferes with fundamental biological processes associated with functional and structural changes in cell surface membranes, cell surface molecules and in their interaction. Based on the data and on their interpretation, we hypothesized that microgravity in addition to observed functional changes affects programmed cell death (PCD) in lymphocyte populations and that this mechanism could contribute to the impairment of the immunity.
Risin, Diana↗
Experimental Study of a Membrane Antenna Surface Adaptive Control System
Due to their ultra lightweight and high packaging efficiency, membrane reflectors are getting more and more attentions for mission architectures that need extremely large inspace deployable antennas. However how to maintain the surface shape of a membrane reflector to the instrument precision requirements is a very challenging problem. This experimental study investigated using PVDF membrane piezoelectric material as actuators to control the surface figures of membrane reflectors. The feasibility of this approach is demonstrated by several sets of test results.
packaging↗
Multiplexed Colorimetric Solid-Phase Extraction
Multiplexed colorimetric solid-phase extraction (MC-SPE) is an extension of colorimetric solid-phase extraction (C-SPE) an analytical platform that combines colorimetric reagents, solid phase extraction, and diffuse reflectance spectroscopy to quantify trace analytes in water. In CSPE, analytes are extracted and complexed on the surface of an extraction membrane impregnated with a colorimetric reagent. The analytes are then quantified directly on the membrane surface using a handheld diffuse reflectance spectrophotometer. Importantly, the use of solid-phase extraction membranes as the matrix for impregnation of the colorimetric reagents creates a concentration factor that enables the detection of low concentrations of analytes in small sample volumes. In extending C-SPE to a multiplexed format, a filter holder that incorporates discrete analysis channels and a jig that facilitates the concurrent operation of multiple sample syringes have been designed, enabling the simultaneous determination of multiple analytes. Separate, single analyte membranes, placed in a readout cartridge create unique, analyte-specific addresses at the exit of each channel. Following sample exposure, the diffuse reflectance spectrum of each address is collected serially and the Kubelka-Munk function is used to quantify each water quality parameter via calibration curves. In a demonstration, MC-SPE was used to measure the pH of a sample and quantitate Ag(I) and Ni(II).
Gazda, Daniel B.↗
Self-Cleaning Tubular-Membrane Module
Tubular membranes made self-cleaning with aid of flow reversing valve. Sponge balls scrub membrane surfaces as they travel inside membrane tubes. A four-way flow-reversal valve automatically reverses flow in tubes at preset intervals so sponge balls reciprocate along tubes. Baskets at ends of tubes prevent sponges from escaping. Automatic cleaning feature added to existing membrane processing equipment with minimal modifications.
Sarbolouki, M. N.↗
Model system for studies of microbial dynamics at exuding surfaces such as the rhizosphere
An autoclavable all-glass system for studying microbial dynamics at permeable surfaces is described. Standard hydrophobic or hydrophilic membranes (46-mm diameter) of various pore sizes were supported on a glass frit through which nutrient solutions were pumped by a peristaltic pump. The pump provided a precisely controlled flow at speeds of 0.5 to 500 ml of defined or natural cell exudates per h, which passed through the membrane into a receiving vessel. The construction allowed a choice of membranes, which could be modified. The system was tested with a bacterium, isolated from rape plant roots (Brassica napus L.), that was inoculated on a hydrophilic membrane filter and allowed to develop into a biofilm. A defined medium with a composition resembling that of natural rape root exudate was pumped through the membrane at 0.5 ml/h. Scanning electron microscopic examinations indicated that the inoculum formed microcolonies embedded in exopolymers evenly distributed over the membrane surface. The lipid composition and content of poly-beta-hydroxybutyrate in free-living and adhered cells were determined by gas chromatography. The bacterial consumption of amino acids in the exudate was also studied.
NASA Discipline Number 61-10↗
The Development of Large Inflatable Antenna for Deep-Space Communications
NASA/JPL's deep-space exploration program has been placing emphasis on reducing the mass and stowage volume of its spacecraft's high-gain and large-aperture antennas. To achieve these goals, the concept of deployable flat reflectarray antenna using an inflatable/thin-membrane structure was introduced at JPL several years ago. A reflectarray is a flat array antenna space-fed by a low-gain feed located at its focal point in a fashion similar to that of a parabolic reflector. The ref1ectarray's elements, using microstrip technology, can be printed onto a flat thin-membrane surface and are each uniquely designed to compensate for the different phase delays due to different path lengths from the feed. Although the reflectarray suffers from limited bandwidth (typically < 10%), it offers a more reliably deployed and maintained flat "natural" surface. A recent hardware development at JPL has demonstrated that a 0.2mm rms surface tolerance (l/50th of a wavelength) was achieved on a 3-meter Ka-band inflatable reflectarray. Another recent development, to combat the reflectarray's narrow band characteristic, demonstrated that dual-band performance, such as X- and Ka-bands, with an aperture efficiency of above 50 percent is achievable by the reflectarray antenna. To mechanically deploy the antenna, the reflectarray's thin membrane aperture surface is supported, tensioned and deployed by an inflatable tubular structure. There are several critical elements and challenging issues associated with the inflatable tube structure. First, the inflatable tube must be made rigidizable so that, once the tube is fully deployed in space, it rigidizes itself and the inflation system is no longer needed. In addition, if the tube is penetrated by small space debris, the tube will maintain its rigidity and not cause deformation to the antenna structure. To support large apertures (e.g. 10m or beyond) without causing any buckling to the small-diameter inflatable tube during vibration, the tube, in addition to rigidization, is also reinforced by circumferential thin blades, as well as axial blades. Second, a controlled deployment mechanism, such as by using Velcro strips, must also be implemented into the system so that, for very large structures, the long inflatable tubes can be deployed in a time-controlled fashion and not get tangled with each other. Third, the thermal analysis is another critical element and must be performed for the tube design in order to assure that the inflated tube, under extreme space thermal conditions, will not deform significantly. Finally, the dynamic vibration analysis must also be performed on the inflatable structure. This will investigate the response of the structure due to excitation introduced by the spacecraft maneuvering and thus determine any necessary damping. Several reflectarray antennas have been developed at JPL to demonstrate the technology. These include an earlier 1-meter X-band inflatable reflectarray, a 3-meter Ka-band inflatable reflectarray, a half-meter dual-band (X and Ka) reflectarray, and the current on-going 10-meter inflatable structure development. The detailed RF and mechanical descriptions of these antennas, as well as their performances, will be presented during the conference.
inflatable antenna↗
Negative regulators of cell proliferation
Cell proliferation is governed by the influence of both mitogens and inhibitors. Although cell contact has long been thought to play a fundamental role in cell cycling regulation, and negative regulators have long been suspected to exist, their isolation and purification has been complicated by a variety of technical difficulties. Nevertheless, over recent years an ever-expanding list of putative negative regulators have emerged. In many cases, their biological inhibitory activities are consistent with density-dependent growth inhibition. Most likely their interactions with mitogenic agents, at an intracellular level, are responsible for either mitotic arrest or continued cell cycling. A review of naturally occurring cell growth inhibitors is presented with an emphasis on those factors shown to be residents of the cell surface membrane. Particular attention is focused on a cell surface sialoglycopeptide, isolated from intact bovine cerebral cortex cells, which has been shown to inhibit the proliferation of an unusually wide range of target cells. The glycopeptide arrest cells obtained from diverse species, both fibroblasts and epithelial cells, and a broad variety of transformed cells. Signal transduction events and a limited spectrum of cells that are refractory to the sialoglycopeptide have provided insight into the molecular events mediated by this cell surface inhibitor.
Review↗
Membrane with internal passages to permit fluid flow and an electrochemical cell containing the same
The invention provides an improved proton exchange membrane for use in electrochemical cells having internal passages parallel to the membrane surface, an apparatus and process for making the membrane, membrane and electrode assemblies fabricated using the membrane, and the application of the membrane and electrode assemblies to a variety of devices, both electrochemical and otherwise. The passages in the membrane extend from one edge of the membrane to another and allow fluid flow through the membrane and give access directly to the membrane for purposes of hydration.
Cisar, Alan J.↗
Gas-phase hydrogen permeation through alpha-titanium - Surface film and dimensional effects
The two techniques normally used to determine diffusivity and permeability in metals are considered and a description is presented of experiments which had been conducted to determine the direct effect of oxide and nitride films on the membrane surface as a function of location and thickness. The test specimens used in the experiments has been machined from commercially pure titanium rod. The results of the experiments show that hydrogen transport in alpha-titanium is partially or completely controlled by surface reactions depending upon membrane wall thickness and the composition and thickness of the surface film.
Shah, K. K.↗
Experimental investigation of drag on a compliant surface
The feasibility of reducing turbulent skin-friction drag by means of surface compliance was studied experimentally in a fully developed flat-plate turbulent boundary layer in air, using a membranous surface backed by a thin cavity containing a layer of polyurethane foam. Surface motion characteristics, boundary layer structure, and overall drag were measured over a range of freestream speeds from 7 to 30 m/sec, and a range of membrane tensions from 44 to 350 N/m. Low-amplitude long-wavelength motions predominate, and no significant change from the rigid surface skin-friction coefficients was observed.
Mcmichael, J. M.↗
Permeability of membranes to amino acids and modified amino acids: mechanisms involved in translocation
The amino acid permeability of membranes is of interest because they are one of the key solutes involved in cell function. Membrane permeability coefficients (P) for amino acid classes, including neutral, polar, hydrophobic, and charged species, have been measured and compared using a variety of techniques. Decreasing lipid chain length increased permeability slightly (5-fold), while variations in pH had only minor effects on the permeability coefficients of the amino acids tested in liposomes. Increasing the membrane surface charge increased the permeability of amino acids of the opposite charge, while increasing the cholesterol content decreased membrane permeability. The permeability coefficients for most amino acids tested were surprisingly similar to those previously measured for monovalent cations such as sodium and potassium (approximately 10(-12)-10(-13) cm s-1). This observation suggests that the permeation rates for the neutral, polar and charged amino acids are controlled by bilayer fluctuations and transient defects, rather than partition coefficients and Born energy barriers. Hydrophobic amino acids were 10(2) more permeable than the hydrophilic forms, reflecting their increased partition coefficient values. External pH had dramatic effects on the permeation rates for the modified amino acid lysine methyl ester in response to transmembrane pH gradients. It was established that lysine methyl ester and other modified short peptides permeate rapidly (P = 10(-2) cm s-1) as neutral (deprotonated) molecules. It was also shown that charge distributions dramatically alter permeation rates for modified di-peptides. These results may relate to the movement of peptides through membranes during protein translocation and to the origin of cellular membrane transport on the early Earth.
Review↗
Polymer electrolyte membrane assembly for fuel cells
An electrolyte membrane for use in a fuel cell can contain sulfonated polyphenylether sulfones. The membrane can contain a first sulfonated polyphenylether sulfone and a second sulfonated polyphenylether sulfone, wherein the first sulfonated polyphenylether and the second sulfonated polyphenylether sulfone have equivalent weights greater than about 560, and the first sulfonated polyphenylether and the second sulfonated polyphenylether sulfone also have different equivalent weights. Also, a membrane for use in a fuel cell can contain a sulfonated polyphenylether sulfone and an unsulfonated polyphenylether sulfone. Methods for manufacturing a membrane electrode assemblies for use in fuel cells can include roughening a membrane surface. Electrodes and methods for fabricating such electrodes for use in a chemical fuel cell can include sintering an electrode. Such membranes and electrodes can be assembled into chemical fuel cells.
Yen, Shiao-Ping S.↗