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At least 19 records

Single-base-pair discrimination of terminal mismatches by using oligonucleotide microarrays and neural network analyses

The effects of single-base-pair near-terminal and terminal mismatches on the dissociation temperature (T(d)) and signal intensity of short DNA duplexes were determined by using oligonucleotide microarrays and neural network (NN) analyses. Two perfect-match probes and 29 probes having a single-base-pair mismatch at positions 1 to 5 from the 5' terminus of the probe were designed to target one of two short sequences representing 16S rRNA. Nonequilibrium dissociation rates (i.e., melting profiles) of all probe-target duplexes were determined simultaneously. Analysis of variance revealed that position of the mismatch, type of mismatch, and formamide concentration significantly affected the T(d) and signal intensity. Increasing the concentration of formamide in the washing buffer decreased the T(d) and signal intensity, and it decreased the variability of the signal. Although T(d)s of probe-target duplexes with mismatches in the first or second position were not significantly different from one another, duplexes with mismatches in the third to fifth positions had significantly lower T(d)s than those with mismatches in the first or second position. The trained NNs predicted the T(d) with high accuracies (R(2) = 0.93). However, the NNs predicted the signal intensity only moderately accurately (R(2) = 0.67), presumably due to increased noise in the signal intensity at low formamide concentrations. Sensitivity analysis revealed that the concentration of formamide explained most (75%) of the variability in T(d)s, followed by position of the mismatch (19%) and type of mismatch (6%). The results suggest that position of the mismatch at or near the 5' terminus plays a greater role in determining the T(d) and signal intensity of duplexes than the type of mismatch.

Non-NASA Center↗

Radio metric errors due to mismatch and offset between a DSN antenna beam and the beam of a troposphere calibration instrument

Two components of the error of a troposphere calibration measurement were quantified by theoretical calculations. The first component is a beam mismatch error, which occurs when the calibration instrument senses a conical volume different from the cylindrical volume sampled by a Deep Space Network (DSN) antenna. The second component is a beam offset error, which occurs if the calibration instrument is not mounted on the axis of the DSN antenna. These two error sources were calculated for both delay (e.g., VLBI) and delay rate (e.g., Doppler) measurements. The beam mismatch error for both delay and delay rate drops rapidly as the beamwidth of the troposphere calibration instrument (e.g., a water vapor radiometer or an infrared Fourier transform spectrometer) is reduced. At a 10-deg elevation angle, the instantaneous beam mismatch error is 1.0 mm for a 6-deg beamwidth and 0.09 mm for a 0.5-deg beam (these are the full angular widths of a circular beam with uniform gain out to a sharp cutoff). Time averaging for 60-100 sec will reduce these errors by factors of 1.2-2.2. At a 20-deg elevation angle, the lower limit for current Doppler observations, the beam-mismatch delay rate error is an Allan standard deviation over 100 sec of 1.1 x 10(exp -14) with a 4-deg beam and 1.3 x 10(exp -l5) for a 0.5-deg beam. A 50-m beam offset would result in a fairly modest (compared to other expected error sources) delay error (less than or equal to 0.3 mm for 60-sec integrations at any elevation angle is greater than or equal to 6 deg). However, the same offset would cause a large error in delay rate measurements (e.g., an Allan standard deviation of 1.2 x 10(exp -14) over 100 sec at a 20-deg elevation angle), which would dominate over other known error sources if the beamwidth is 2 deg or smaller. An on-axis location is essential for accurate troposphere calibration of delay rate measurements. A half-power beamwidth (for a beam with a tapered gain profile) of 1.2 deg or smaller is desired for calibration of all types of radio metrics. A water-vapor radiometer calibration beam of this size with very low sidelobes would require a clear aperture antenna with a diameter of at least 1.5 m if the primary water vapor sensing channel were in the 20-22 GHz range.

R P Linfield↗

A Direct Adaptive Control Approach in the Presence of Model Mismatch

This paper considers the problem of direct model reference adaptive control when the plant-model matching conditions are violated due to abnormal changes in the plant or incorrect knowledge of the plant's mathematical structure. The approach consists of direct adaptation of state feedback gains for state tracking, and simultaneous estimation of the plant-model mismatch. Because of the mismatch, the plant can no longer track the state of the original reference model, but may be able to track a new reference model that still provides satisfactory performance. The reference model is updated if the estimated plant-model mismatch exceeds a bound that is determined via robust stability and/or performance criteria. The resulting controller is a hybrid direct-indirect adaptive controller that offers asymptotic state tracking in the presence of plant-model mismatch as well as parameter deviations.

Joshi, Suresh M.↗

Decentralized Adaptive Control of Systems with Uncertain Interconnections, Plant-Model Mismatch and Actuator Failures

Decentralized adaptive control is considered for systems consisting of multiple interconnected subsystems. It is assumed that each subsystem s parameters are uncertain and the interconnection parameters are not known. In addition, mismatch can exist between each subsystem and its reference model. A strictly decentralized adaptive control scheme is developed, wherein each subsystem has access only to its own state but has the knowledge of all reference model states. The mismatch is estimated online for each subsystem and the mismatch estimates are used to adaptively modify the corresponding reference models. The adaptive control scheme is extended to the case with actuator failures in addition to mismatch.

Patre, Parag↗

Working at the Speed of Innovation: Impedance Mismatch in Rapid and Innovation Projects

In this paper we report on the results of an ethnographic study of a rapid design innovation (RDI) experiment in NASA Aeronautics. This work is based on the study of the Aeronautics Autonomy Testbed Capability (AATC) team in the Convergent Aeronautics Solutions (CAS) project. This paper focuses on and summarizes one of the key over-arching findings from the study: there is a significant mismatch in the organizational culture in the rest of the organization compared with that required for RDI. And, if this organizational cultural mismatch is not addressed, the likelihood of any organization being able to advance a new and different type of work (such as RDI) will be jeopardized. We delineate several aspects of the two different cultures identified in order to enable leaders and practitioners to better understand what contributes to the cultural dissonance and the implications of the differences in the cultures. As well, we identify ways in which those differences can be addressed. Research from organization and other social sciences are incorporated to highlight the differences. The implications of the research suggest that the significant cultural differences trigger a strong and resistive response from the dominant culture that may negate leadership’s strategy to build the new capacity for RDI. While the research results noted the pervasive nature of innovation throughout the workplace, the type of innovation envisioned in RDI is a rare type of innovation that requires significantly new methods, work processes, tools, and skills such that approaches used in the dominate culture cannot be adopted by expediting the existing approaches. Examples include: innovation teaming and leadership; the need for adaptive leadership that changes the relationship of a research leader to the other researchers; and, an interdisciplinary teaming approach which shapes team relationships and activities. Each of these aspects requires new teaming, tools, and skills in order to succeed. Thus, when introducing RDI activities where there is a different dominant culture, teams need to be: well trained; protected; recognized and rewarded. And, team leaders must also be trained in the unique types of teaming and innovation tools used in RDI. Both RDI teaming and team leadership must be sanctioned, supported, and rewarded by leadership. Because of the cultural mismatch, in some organizations looking to add RDI to their existing and established organizations, sep to avoid or mitigate the negative impact of the culture mismatch.

Crain, Cathleen↗

Five amino acid mismatches in the zinc-finger domains of Cellulose Synthase 5 and Cellulose Synthase 6 cooperatively modulate their functional properties by controlling homodimerization in Arabidopsis

Cellulose synthase 5 (CESA5) and CESA6 are known to share substantial functional overlap. In the zinc-finger domain (ZN) of CESA5, there are five amino acid (AA) mismatches when compared to CESA6. These mismatches in CESA5 were replaced with their CESA6 counterparts one by one until all were replaced, generating nine engineered CESA5s. Each N-terminal enhanced yellow fluorescent protein-tagged engineered CESA5 was introduced to prc1-1, a cesa6 null mutant, and resulting mutants were subjected to phenotypic analyses. For this work, we found that five single AA-replaced CESA5 proteins partially rescue the prc1-1 mutant phenotypes to different extents. Multi-AA replaced CESA5s further rescued the mutant phenotypes in an additive manner, culminating in full recovery by CESA5 G43R + S49T+S54P+S80A+Y88F . Investigations in cellulose content, cellulose synthase complex (CSC) motility, and cellulose microfibril organization in the same mutants support the results of the phenotypic analyses. Bimolecular fluorescence complementation assays demonstrated that the level of homodimerization in every engineered CESA5 is substantially higher than CESA5. The mean fluorescence intensity of CSCs carrying each engineered CESA5 fluctuates with the degree to which the prc1-1 mutant phenotypes are rescued by introducing a corresponding engineered CESA5. Taken together, these five AA mismatches in the ZNs of CESA5 and CESA6 cooperatively modulate the functional properties of these CESAs by controlling their homodimerization capacity, which in turn imposes proportional changes on the incorporation of these CESAs into CSCs.

59 BASIC BIOLOGICAL SCIENCES↗

Optimization of single-base-pair mismatch discrimination in oligonucleotide microarrays

The discrimination between perfect-match and single-base-pair-mismatched nucleic acid duplexes was investigated by using oligonucleotide DNA microarrays and nonequilibrium dissociation rates (melting profiles). DNA and RNA versions of two synthetic targets corresponding to the 16S rRNA sequences of Staphylococcus epidermidis (38 nucleotides) and Nitrosomonas eutropha (39 nucleotides) were hybridized to perfect-match probes (18-mer and 19-mer) and to a set of probes having all possible single-base-pair mismatches. The melting profiles of all probe-target duplexes were determined in parallel by using an imposed temperature step gradient. We derived an optimum wash temperature for each probe and target by using a simple formula to calculate a discrimination index for each temperature of the step gradient. This optimum corresponded to the output of an independent analysis using a customized neural network program. These results together provide an experimental and analytical framework for optimizing mismatch discrimination among all probes on a DNA microarray.

NASA Discipline Life Sciences Technologies↗

Charge Density Mismatch is a Key Characteristic of Highly Concentrated Electrolyte Solutions and Highly Water‐Soluble Salts

Only very soluble electrolytes can form concentrated solutions. Some salts are so soluble that there are less than four water molecules per ion in saturated solution. Ions usually form clusters or networks with more than one counterion in their coordination sphere in these concentrated solutions. Do these ultraconcentrated solutions form because the counterions have high affinity for each other in liquid, or because they have a poor affinity for each other in solids? Here, in this study, this question is addressed using the valence matching principle of the bond valence model by comparing the charge density mismatch between counterions to their solubility for a series of alkali fluorides, carboxylates, and oxyanions. The solubilities are plotted against the characteristic average bond valence of the alkali, and the lowest solubilities are those where alkali and anion had matching bond valences. Conversely, the highest solubilities are those with poorly matching bond valences. Available ion-pairing constants indicate that the weakest ion-pairs are those with the largest bond valence mismatch, indicating that the large water solubilities occur despite weak ion-pairing rather than because of strong ion-pairing. Therefore, a key characteristic of highly water-soluble salts is that the counterions have mismatched charge densities.

concentrated solutions↗

Radiation of cylindrical duct acoustic modes with flow mismatch

Motivated by the problem of Mach number and temperature discontinuity mismatch between the tailpipe and the surrounding medium, and the effect of this flow mismatch on aircraft engine noise, the radiation of spinning turbo-acoustic modes from a round duct is investigated. The model, based on a plug flow exhaust jet, solves the convected wave equations using Fourier transforms, inversion being accomplished via a coupled Wiener-Hopf and Carrier-Koiter technique. It is concluded that the Mach number discontinuity results in a conflict between convection and refraction effects, while the temperature mismatch results in a sharp beaming of sound off to the side.

Savkar, S. D.↗

Calibration of solar cells by the reference cell method - The spectral mismatch problem

The calibration of solar cells by means of solar simulators and calibrated reference cells has always been faced with the problem of errors due to source spectral irradiance mismatch and cell spectral response mismatch. A general expression is derived in this paper to enable calculation of mismatch error. The information required to make this calculation is the relative spectra responses of the test and reference cells, and the relative spectral irradiance of the simulator (over the spectral range defined by cell response). The spectral irradiance of the solar standard is assumed given. Experimental results are presented.

Seaman, C. H.↗

Temperature dependence of gamma-gamma prime lattice mismatch in nickel-base superalloys

High temperature X-ray diffraction techniques were used to determine the gamma-gamma prime lattice mismatch of three different nickel-base superalloys at temperatures between 18 and 1000 C. The measurements were performed on oriented single-crystal disks which had been aged to produce a semicoherent gamma-gamma prime structure. The thermal expansion of the lattice parameters of the gamma and gamma-prime phases was described by a second-order polynomial expression. The expansion of the gamma-prime phase was consistently smaller than that of the gamma phase, which caused the lattice mismatch to become more negative at higher temperatures. It was also shown that high values of lattice mismatch resulted in increased rates of directional gamma-prime coarsening during elevated temperature creep exposure.

Nathal, M. V.↗

The Effects of Composition and gamma'/gamma Lattice Parameter Mismatch on the Critical Resolved Shear Stresses for Octahedral and Cube Slip in NiAlCrX Alloys

Prototypical single-crystal NiAlCrX superalloys were studied to examine the effects of the common major alloying elements, Co, Mo, Nb, Ta, Ti, and W, on yielding behavior. The alloys contained about 10 at. pct Cr, 60 vol pct of the gamma' phase, and about 3 at. pct of X in the gamma'. The critical resolved shear stresses (CRSSs) for octahedral and primary cube slip were measured at 760 C, which is about the peak strength temperature. The CRSS(sub oct) and CRSS(sub cube) are discussed in relation to those of Ni, (Al, X) gamma' alloys taken from the literature and the gamma'/gamma lattice mismatch. The CRSS(sub oct) of the gamma + gamma' alloys reflected a similar compositional dependence to that of both the CRSS(sub cube) of the gamma' phase and the gamma'/gamma lattice parameter mismatch. The CRSS(sub cube) of the gamma + gamma' alloys also reflected the compositional dependence of the gamma'/gamma mismatch, but bore no similarity to that of CRSS(sub cube) for gamma' alloys since it is controlled by the gamma matrix. The ratio of CRSS(sub cube)/CRSS(sub oct) was decreased by all alloying elements except Co, which increased the ratio. The decrease in CRSS(sub cube)/CRSS(sub oct) was related to the degree in which elements partition to the gamma' rather than the gamma phase.

Miner, R. V.↗

A Neural Network-Based Power Mismatch Elimination Strategy for Integrated Solar and ESS AC/DC Systems (MARS)

The multiport autonomous reconfigurable solar power plant (MARS) is a promising concept for the integration of photovoltaic (PV) and energy storage system (ESS) to the transmission ac grid and a high-voltage direct current (HVdc) link. The presence of PV and ESS in each arm of the MARS results in uneven distribution of active power among different submodules (SMs), thereby leading to unbalanced SM capacitor voltages and potentially compromising the system stability. Moreover, in the case of partial shadings, shaded PV SMs will suffer from decreased power injections causing power mismatch in the MARS system. To address this issue, a neural-network-based power mismatch elimination (NNPME) strategy is proposed in this article. The proposed NNPME strategy optimizes ESS usage and leverages both dc and ac circulating currents to facilitate power transfer among the SMs, arms, and phases of the MARS system. Simulation and control hardware-in-the-loop (cHIL) experiments demonstrate the effectiveness of the proposed NNPME strategy. Compared with the traditional approaches, the proposed NNPME strategy can significantly enhance system efficiency and ensure stable and continuous operation, even in the presence of uneven power distribution within the MARS system.

14 SOLAR ENERGY↗

Polarization mismatch errors in radio phase interferometers.

An analysis is presented which deals with the effects of polarization mismatch errors on the accuracy of a phase interferometer used for position location of unknown emitters relative to known calibration emitters. Closed-form expressions for the induced phase difference between interferometer antennas are derived for several combinations of receiving and transmitting antenna polarizations. Errors contributed by mechanical misalignment between antennas, as well as effects of power loss attributable to polarization mismatch, are also considered. The analysis leads to the conclusion that circularly polarized interferometer and transmitter antennas are best suited for the position location application, if it is assumed that polarization tracking of the interferometer antennas is not available. It is shown that a reasonable amount of ellipticity can be tolerated before the phase error becomes significant.

Muehldorf, E. I.↗

The effect of a visual/motion display mismatch in a single axis compensatory tracking task

An experiment was performed to determine the effect of a performance mismatch between the visual and motion display systems on a real time piloted aircraft simulation. Pilots performed a compensatory roll tracking task with dynamics typical of medium jet transports. Between 0 and 10 rad/sec, visual and motion system responses were equivalent to either unity or a first order lag at 4.8 rad/sec. Pilot describing functions and error scores were calculated. Results show that the mismatch between visual and motion display systems has no significant effect. It is the absence of high frequency visual and/or motion cues which significantly affects pilot performance.

Shirachi, D. K.↗

In-flight simulation with pilot-center of gravity offset and velocity mismatch

Similarity transformations which preserve modal characteristics and pilot's acceleration cues in in-flight simulation are presented. The model transformation for lateral acceleration matching is developed. A velocity-mismatch example, based on a VRA simulation of the Space Shuttle, illustrates that acceleration matching is achieved at the expense of mismatching in cues which are secondary to the simulated piloting task, while primarily cues are preserved. The approach is applicable for both implicit and explicit model-following, and it can easily be extended to the longitudinal case.

Stengel, R. F.↗

The impact of mismatch on the performance of coded narrow-band FM with limiter/discriminator detection

An examination of the impact of mismatch on the performance of convolutionally encoded/Viterbi decoded narrow-band FM with limiter/discriminator detection is presented. Attention was given to the potential gain available by the combination of this type of system in terms of hard and soft decision decoding. Soft decision decoding was demonstrated to offer only approximately 0.3 dB better performance than hard decision coding. It was also shown, through a technique involving the number of clicks occurring in each detection interval, that both soft and hard decision decoding bit error probability performance could be improved. It is concluded that the mismatch between the coding channel and the decoding metric of the Viterbi algorithm is responsible for reducing the difference between hard and soft decoding metrics.

Simon, M. K.↗

A review and summary of cell and module mismatch losses from theoretical and experimental studies

Studies on power loss due to photovoltaic (PV) cell and module parameter mismatch were reviewed. The losses result from the current voltage (I-V) characteristics of cells which vary over a manufactured lot. The peak power and the peak power I-V point vary from cell to cell. The largest parameter variation occurs in the cell photocurrent generation and the most significant loss occurs in series connections since all cells are essentially limited by the lowest photocurrent. Most theoretical studies address only photocurrent variation effects, which consider shunt and series resistance, ideality factor and saturation current to be comparatively insignificant. One study is identified which specifically configured the study of mismatch loss effects. Experimental results consist of estimates based on limited data not taken from specially configured tests.

Lambarski, T. J.↗