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At least 19 records

Non-invasive method and apparatus for measuring pressure within a pliable vessel

A non-invasive method and apparatus is disclosed for measuring pressure within a pliable vessel such as a blood vessel. The blood vessel is clamped by means of a clamping structure having a first portion housing a pressure sensor and a second portion extending over the remote side of the blood vessel for pressing the blood vessel into engagement with the pressure sensing device. The pressure sensing device includes a flat deflectable diaphragm portion arranged to engage a portion of the blood vessel flattened against the diaphragm by means of the clamp structure. In one embodiment, the clamp structure includes first and second semicylindrical members held together by retaining rings. In a second embodiment the clamp structure is of one piece construction having a solid semicylindrical portion and a hollow semicylindrical portion with a longitudinal slot in the follow semicylindrical portion through which a slip the blood vessel. In a third embodiment, an elastic strap is employed for clamping the blood vessel against the pressure sensing device.

Shimizu, M.↗

The identification of the variation of atherosclerosis plaques by invasive and non-invasive methods

Computer-enhanced visualization of coronary arteries and lesions within them is discussed, comparing invasive and noninvasive methods. Trial design factors in computer lesions assessment are briefly discussed, and the use of the computer edge-tracking technique in that assessment is described. The results of a small pilot study conducted on serial cineangiograms of men with premature atherosclerosis are presented. A canine study to determine the feasibility of quantifying atherosclerosis from intravenous carotid angiograms is discussed. Comparative error for arterial and venous injection in the canines is determined, and the mode of processing the films to achieve better visualization is described. The application of the computer edge-tracking technique to an ultrasound image of the human carotid artery is also shown and briefly discussed.

Selzer, R. H.↗

A new approach to non-invasive oxygenated mixed venous PCO(sub)2

A clinically practical technique was developed to calculate mixed venous CO2 partial pressure for the calculation of cardiac output by the Fick technique. The Fick principle states that the cardiac output is equal to the CO2 production divided by the arterio-venous CO2 content difference of the pulmonary vessels. A review of the principles involved in the various techniques used to estimate venous CO2 partial pressure is presented.

Fisher, Joseph A.↗

A non-invasive measure of minerals and electrolytes in tissue

A system for collecting epithelial cells from the oral mucosa for the determination of ion concentration is discussed with application to the study of man's adaptation to microgravity. A number of characteristics of these cells influenced the choice for clinical testing. They are non-cornified epithelial cells located on the inferior aspect of the tongue; therefore, they are well protected from trauma. They have the capability of reflecting relatively recent physiologic changes since they are renewed every three days and have aerobic metabolism. Most importantly, they are easily accessible and can be removed by a wooden applicator stick with minimum discomfort. Smears of cells removed in this manner show predominantly individual cells rather than sheets of contiuous cells. This facilitates the visual isolation of single cells with the electron microscope for analysis. NASA's principle effort in the development of a test to measure the ion concentration in sublingual cells has been research by the biomedical program carried out by scientists with expertise in skeletal metabolism. These efforts were directed toward determining the biological meaning and deviations in interacellular ions in nonhuman primates and in male volunteers for experiments in a model for weightlessness. A brief one page summary of the experiments and results are presented.

Arnaud, Sara B.↗

Continuous cardiac output measurement - Aspects of Doppler frequency analysis

From the suprasternal notch blood flow velocity in the aorta can be measured non-invasively by a Doppler probe. Integration over systole after frequency analysis gives a measure of stroke volume if a separate diameter observation is incorporated. Frequency analysis by a zero crossing counter or by a set of parallel phaselock loops was less effective than a set of bandpass filters. Observations on dogs, baboons and humans before and after exercise or surgery suggest the indications to be useful. Application to judging heart failure by the effect of introducing a volume load is indicated. Changes in output also are measured in freely moving subjects.

Mackay, R. S.↗

Monitoring the state of the human airways by analysis of respiratory sound

A mechanism whereby sound is generated by the motion of vortices in the human lung is described. This mechanism is believed to be responsible for most of the sound which is generated both on inspiration and expiration in normal lungs. Mathematical expressions for the frequencies of sound generated, which depend only upon the axial flow velocity and diameters of the bronchi, are derived. This theory allows the location within the bronchial tree from which particular sounds emanate to be determined. Redistribution of pulmonary blood volume following transition from earth gravity to the weightless state probably alters the caliber of certain airways and doubtless alters sound transmission properties of the lung. We believe that these changes can be monitored effectively and non-invasively by spectral analysis of pulmonary sound.

Hardin, J. C.↗

Urine collection apparatus

A urine collection device for females comprises an interface body with an interface surface for engagement with the user's body. The interface body comprises a forward portion defining a urine-receiving bore which has an inlet in the interface surface adapted to be disposed in surrounding relation to the urethral opening of the user. The interface body also has a rear portion integrally adjoining the forward portion and a non-invasive vaginal seal on the interface surface for sealing the vagina of the user from communication with the urine-receiving bore. An absorbent pad is removably supported on the interface body and extends laterally therefrom. A garment for supporting the urine collection is also disclosed.

Michaud, R. B.↗

Cardiac chamber volumes by echocardiography using a new mathematical method: A promising technique for zero-G use

Accurate, reproducible, and non-invasive means for ventricular volume determination are needed for evaluating cardiovascular function zero-gravity. Current echocardiographic methods, particularly for the right ventricle, suffer from a large standard error. A new mathematical approach, recently described by Watanabe et al., was tested on 1 normal formalin-fixed human hearts suspended in a mineral oil bath. Volumes are estimated from multiple two-dimensional echocardiographic views recorded from a single point at sequential angles. The product of sectional cavity area and center of mass for each view summed over the range of angles (using a trapezoidal rule) gives volume. Multiple (8-14) short axis right ventricle and left ventricle views at 5.0 deg intervals were videotaped. The images were digitized by two independent observers (leading-edge to leading-edge technique) and analyzed using a graphics tablet and microcomputer. Actual volumes were determined by filling the chambers with water. These data were compared to the mean of the two echo measurements.

Buckey, J. C.↗

Analysis of holographic interferograms of aerodynamic models in a wind tunnel

Holographic interferometry provides a non-invasive technique for estimating variations in the air density distribution around aerodynamic models in wind tunnels. The testing of this technique has been underway for some time and has been reported previously for a two dimensional aerodynamic model. Results obtained from tests using three dimensional aerodynamic models are summarized. Holograms were made of aerodynamic models in a wind tunnel. Interferograms were made from these holograms. The interference fringes in these holographic interferograms were digitized and this information was entered into the HOLOFT program. The HOLOFT program successfully calculated the known stagnation air density at the nose of a model and the known air density distribution across the cross section passing through the stagnation point for the axisymmetrical case of this model at a Mach number of 0.8. Thus the technique of holographic interferometry does work.The HOLOFT program stands for HOLOgraphic Inversion by 2-D Fourier Transform.

Perry, R. L.↗

How to detect when cells in space perceive gravity

It is useful to be able to measure when and whether cells detect gravity during spaceflights. For studying gravitational physiology, gravity perception is the response the experimentalist needs to measure. Also, for growing plants in space, plant cells may have a non-directional requirement for gravity as a development cue. The main goals of spaceflight experiments in which gravity perception would be measured are to determine the properties of the gravity receptor and how it is activated, and to determine fundamental characteristics of the signal generated. The main practical difficulty with measuring gravity sensing in space is that gravity sensing cannot be measured with certainty on earth. Almost all experiments measure gravitropic curvature. Reciprocity and intermittent stimulation are measurements which were made to some degree on earth using clinostatting, but which would provide clearer results if done with microgravity rather than clinostatting. These would be important uses of the space laboratory for determining the nature of gravity sensing in plants. Those techniques which do not use gravitropic curvature to measure gravity sensing are electrophysiological. The vibrating probe would be somewhat easier to adapt to space conditions than the intracellular microelectrode because it can be positioned with less precision. Ideally, a non-invasive technique would be best suited if an appropriate measure could be developed. Thus, the effect of microgravity on cultured cells is more likely to be by large-scale physical events than gravity sensing in the culture cells. It is not expected that it will be necessary to determine whether individual cultured cells perceive gravity unless cells grow abnormally even after the obvious microgravity effects on the culture as a whole can be ruled out as the cause.

Bjoerkman, Thomas↗

Conservation of body calcium by increased dietary intake of potassium: A potential measure to reduce the osteoporosis process during prolonged exposure to microgravity

During the 1988 NASA Summer Faculty Fellowship Program, it was proposed that the loss of skeletal calcium upon prolonged exposure to microgravity could be explained, in part, by a renal maladjustment characterized by an increased urinary excretion of calcium. It was theorized that because the conservation of body fluids and electrolytes depends upon the energy of adenosine triphosphate and enzymes that control the use of its energy for renal ion transport, an induction of renal sodium and potassium-dependent adenosine triphosphatase (Na + K ATPase) by oral loading with potassium would increase the reabsorption of sodium directly and that of calcium indirectly, leading to improved hydration and to reduced calcium loss. Preliminary studies showed the following. Rats drinking water containing 0.2 M potassium chloride for six to 13 days excreted in urine 22 muEq of calcium and 135 muEq of sodium per 100 grams of body weight per day. The corresponding values for control rats drinking tap water were 43 muEq and 269 muEq respectively. Renal Na + K ATPase activity in potassium loaded rats was higher than in controls. Thus, oral potassium loading resulted in increased Na + K ATPase activity and diminished urinary excretion of calcium and of sodium as predicted by the hypothesis. An extension of these studies to humans has the potential of resulting in development of harmless, non-invasive, drug-free, convenient measures to reduce bone loss and other electrolyte and fluid problems in space travelers exposed to prolonged periods of microgravity.

Nechay, Bohdan R.↗

The effect of process conditions on the performance of epitaxial InP solar cells

Indium phosphide solar cells have a higher resistance to electron irradiation than Si or GaAs cells of comparable junction depth. As a result, there is much interest in the use of this material for space applications. Cells of this material were made in bulk InP by a number of techniques, including ion implantation, direct diffusion in sealed ampoules, and by open tube diffusion. However, it is generally considered that the epitaxial approach will be superior to all of these techniques. The epitaxy of InP is considerably more difficult than that of gallium arsenide, for a number of reasons. Perhaps the most important is the fact that the native oxides of Indium are extremely difficult to remove, as compared to that of Gallium. In addition, thermal treatments for the desorption of these oxides often result in the formation of phosphorus vacancies and free indium on the surface. Thus, inadequate sample preparation before epitaxy, poor reactor cleaning procedures, or poor transition procedures between the growth of successive layers, all give rise to trap phenomena and to high interface recombination velocities. Moreover, the lifetime of the grown material is dominated by the occurrence of native defects, so that it is a strong function of growth parameters. These problems are of special interest to the fabrication of solar cells, where long life-time, combined with the absence of traps, is highly desirable. A study of this problem is described using a non-invasive diagnostic technique which was developed.

Borrego, J. M.↗

In-Suit Doppler Technology Assessment

The objective of this program was to perform a technology assessment survey of non-invasive air embolism detection utilizing Doppler ultrasound methodologies. The primary application of this technology will be a continuous monitor for astronauts while performing extravehicular activities (EVA's). The technology assessment was to include: (1) development of a full understanding of all relevant background research; and (2) a survey of the medical ultrasound marketplace for expertise, information, and technical capability relevant to this development. Upon completion of the assessment, LSR was to provide an overview of technological approaches and R&D/manufacturing organizations.

Schulze, Arthur E.↗

Large scale crystallization of protein pharmaceuticals in microgravity via temperature change

The major objective of this research effort is the temperature driven growth of protein crystals in large batches in the microgravity environment of space. Pharmaceutical houses are developing protein products for patient care, for example, human insulin, human growth hormone, interferons, and tissue plasminogen activator or TPA, the clot buster for heart attack victims. Except for insulin, these are very high value products; they are extremely potent in small quantities and have a great value per gram of material. It is feasible that microgravity crystallization can be a cost recoverable, economically sound final processing step in their manufacture. Large scale protein crystal growth in microgravity has significant advantages from the basic science and the applied science standpoints. Crystal growth can proceed unhindered due to lack of surface effects. Dynamic control is possible and relatively easy. The method has the potential to yield large quantities of pure crystalline product. Crystallization is a time honored procedure for purifying organic materials and microgravity crystallization could be the final step to remove trace impurities from high value protein pharmaceuticals. In addition, microgravity grown crystals could be the final formulation for those medicines that need to be administered in a timed release fashion. Long lasting insulin, insulin lente, is such a product. Also crystalline protein pharmaceuticals are more stable for long-term storage. Temperature, as the initiation step, has certain advantages. Again, dynamic control of the crystallization process is possible and easy. A temperature step is non-invasive and is the most subtle way to control protein solubility and therefore crystallization. Seeding is not necessary. Changes in protein and precipitant concentrations and pH are not necessary. Finally, this method represents a new way to crystallize proteins in space that takes advantage of the unique microgravity environment. The results from two flights showed that the hardware performed perfectly, many crystals were produced, and they were much larger than their ground grown controls. Morphometric analysis was done on over 4,000 crystals to establish crystal size, size distribution, and relative size. Space grown crystals were remarkably larger than their earth grown counterparts and crystal size was a function of PCF volume. That size distribution for the space grown crystals was a function of PCF volume may indicate that ultimate size was a function of temperature gradient. Since the insulin protein concentration was very low, 0.4 mg/ml, the size distribution could also be following the total amount of protein in each of the PCF's. X-ray analysis showed that the bigger space grown insulin crystals diffracted to higher resolution than their ground grown controls. When the data were normalized for size, they still indicated that the space crystals were better than the ground crystals.

Long, Marianna M.↗

EVA Glove Research Team

The goal of the basic research portion of the extravehicular activity (EVA) glove research program is to gain a greater understanding of the kinematics of the hand, the characteristics of the pressurized EVA glove, and the interaction of the two. Examination of the literature showed that there existed no acceptable, non-invasive method of obtaining accurate biomechanical data on the hand. For this reason a project was initiated to develop magnetic resonance imaging as a tool for biomechanical data acquisition and visualization. Literature reviews also revealed a lack of practical modeling methods for fabric structures, so a basic science research program was also initiated in this area.

Strauss, Alvin M.↗

Echocardiography in the flight program

Observations on American and Soviet astronauts have documented the association of changes in cardiovascular function during orthostasis with space flight. A basic understanding of the cardiovascular changes occurring in astronauts requires the determination of cardiac output and total peripheral vascular resistance as a minimum. In 1982, we selected ultrasound echocardiography as our means of acquiring this information. Ultrasound offers a quick, non-invasive and accurate means of determining stroke volume which, when combined with the blood pressure and heart rate measurements of the stand test, allows calculation of changes in peripheral vascular resistance, the body's major response to orthostatic stress. The history of echocardiography in the Space Shuttle Program is discussed and the results are briefly presented.

Charles, John B.↗

Workshop on Advances in NASA-Relevant, Minimally Invasive Instrumentation

The purpose of this meeting is to highlight those advances in instrumentation and methodology that can be applied to the medical problems that will be encountered as the duration of manned space missions is extended. Information on work that is presently being done by NASA as well as other approaches in which NASA is not participating will be exchanged. The NASA-sponsored efforts that will be discussed are part of the overall Space Medicine Program that has been undertaken by NASA to address the medical problems of manned spaceflight. These problems include those that have been observed in the past as well as those which are anticipated as missions become longer, traverse different orbits, or are in any way different. This conference is arranged in order to address the types of instrumentation that might be used in several major medical problem areas. Instrumentation that will help in the cardiovascular, musculoskeletal, and psychological areas, among others will be presented. Interest lies in identifying instrumentation which will help in learning more about ourselves through experiments performed directly on humans. Great emphasis is placed on non-invasive approaches, although every substantial program basic to animal research will be needed in the foreseeable future. Space Medicine is a rather small affair in what is primarily an engineering organization. Space Medicine is conducted throughout NASA by a very small skeleton staff at the headquarters office in Washington and by our various field centers. These centers include the Johnson Space Center in Houston, Texas, the Ames Research Center in Moffett Field, California, the Jet Propulsion Laboratory in Pasadena, California, the Kennedy Space Center in Florida, and the Langley Research Center in Hampton, Virginia. Throughout these various centers, work is conducted in-house by NASA's own staff scientists, physicians, and engineers. In addition, various universities, industries, and other government laboratories perform research that cannot be effectively carried out in-house. At the moment, approximately 50% of the work is performed in-house and 50% is extramural. The area of bioinstrumentation pervades every one of our problem areas. In each, equipment or procedures are being developed that will allow more clinical work to be done in a ground-based or spacecraft setting. Although work of this kind goes on throughout the NASA organization and through its grants and contracts in the community at large, the major thrust of it is concentrated at the Jet Propulsion Laboratory which plays a lead role in this type of research and acts as the lead center in bioinstrumentation for NASA. It is recognized that there is much additional research being pursued in this area which would be potentially valuable to NASA and could, with some stimulation from, be made more applicable to NASA's needs. It is hoped, therefore, that the proceedings of this conference will be used as the basis for developing research strategies to be used as a road map to point the way in which NASA's own sponsored program should proceed over the course of the next three years. Additionally, it is hoped that the conference will highlight additional areas in which NASA should be involved either in-house or through the sponsorship of non-NASA scientists. NASA would also like to get an idea of which areas should be emphasized or perhaps de-emphasized among those that it is currently pursuing. In considering these questions, the discussion should concern itself not so much with whether a particular procedure or piece of equipment would work in a spacecraft, but rather, with whether the procedures that are advocated are at the state-of-the-art or beyond the state-of-the-art and whether they hold promise of giving additional insight into the problems to be confronted as humans venture into space for longer and longer periods of time.

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