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At least 19 records

Cell-Detection Technique for Automated Patch Clamping

A unique and customizable machinevision and image-data-processing technique has been developed for use in automated identification of cells that are optimal for patch clamping. [Patch clamping (in which patch electrodes are pressed against cell membranes) is an electrophysiological technique widely applied for the study of ion channels, and of membrane proteins that regulate the flow of ions across the membranes. Patch clamping is used in many biological research fields such as neurobiology, pharmacology, and molecular biology.] While there exist several hardware techniques for automated patch clamping of cells, very few of those techniques incorporate machine vision for locating cells that are ideal subjects for patch clamping. In contrast, the present technique is embodied in a machine-vision algorithm that, in practical application, enables the user to identify good and bad cells for patch clamping in an image captured by a charge-coupled-device (CCD) camera attached to a microscope, within a processing time of one second. Hence, the present technique can save time, thereby increasing efficiency and reducing cost. The present technique involves the utilization of cell-feature metrics to accurately make decisions on the degree to which individual cells are "good" or "bad" candidates for patch clamping. These metrics include position coordinates (x,y) in the image plane, major-axis length, minor-axis length, area, elongation, roundness, smoothness, angle of orientation, and degree of inclusion in the field of view. The present technique does not require any special hardware beyond commercially available, off-the-shelf patch-clamping hardware: A standard patchclamping microscope system with an attached CCD camera, a personal computer with an imagedata- processing board, and some experience in utilizing imagedata- processing software are all that are needed. A cell image is first captured by the microscope CCD camera and image-data-processing board, then the image data are analyzed by software that implements the present machine-vision technique. This analysis results in the identification of cells that are "good" candidates for patch clamping (see figure). Once a "good" cell is identified, a patch clamp can be effected by an automated patchclamping apparatus or by a human operator. This technique has been shown to enable reliable identification of "good" and "bad" candidate cells for patch clamping. The ultimate goal in further development of this technique is to combine artificial-intelligence processing with instrumentation and controls in order to produce a complete "turnkey" automated patch-clamping system capable of accurately and reliably patch clamping cells with a minimum intervention by a human operator. Moreover, this technique can be adapted to virtually any cellular-analysis procedure that includes repetitive operation of microscope hardware by a human.

McDowell, Mark

Reconfigurable Wideband Circularly Polarized Stacked Square Patch Antenna for Cognitive Radios

An almost square patch, a square patch and a stacked square patch with corner truncation for circular polarization (CP) are researched and developed at X-band for cognitive radios. Experimental results indicate, first, that the impedance bandwidth of a CP almost square patch fed from the edge by a 50 ohm line is 1.70 percent and second, that of a CP square patch fed from the ground plane side by a surface launch connector is 1.87 percent. Third, the impedance bandwidth of a CP stacked square patch fed by a surface launch connector is 2.22 percent. The measured center frequency for the CP square patch fed by a surface launch connector without and with an identical stacked patch is 8.45 and 8.1017 GHz, respectively. By stacking a patch, separated by a fixed air gap of 0.254 mm, the center frequency is observed to shift by as much as 348.3 MHz. The shift in the center frequency can be exploited to reconfigure the operating frequency by mechanically increasing the air gap. The results indicate that a tuning bandwidth of about 100 MHz can be achieved when the distance of separation between the driven patch and the stacked patch is increased from its initial setting of 0.254 to 1.016 mm.

Cognitive

Effect of patch borders on coercivity in amorphous rare earth-transition metal thin films

The coercivity at the micron scale is a very important property of magneto-optical media. It is a key factor that determines the magnetic domain wall movement and domain reversal. How the coercivity is influenced by a special type of patch borders is discussed. Patch formation is a general phenomenon in growth processes of amorphous rare earth transition metal thin films. Different patches may stem from different seeds and the patch borders are formed when they merge. Though little is known about the exact properties of the borders, we may expect that the exchange interaction at the patch border is weaker than that within a patch, since there is usually a spatial gap between two patches. Computer simulations were performed on a 2-D hexagonal lattice consisting of 37 complete patches with random shape and size. From the series of simulations we may conclude that the domain in the patch with borders of 30 percent exchange strength can expand most easily to the whole lattice, because the exchange strength can expand most easily to the whole lattice, because the exchange strength of the border is not too high to prevent the domain from growing within the patch and it is not too low to prevent the domain from expanding beyond the patch.

Patterson, G.

Analysis of Stub Loaded Microstrip Patch Antennas

A microstrip patch antenna fed by a coaxial probe and reactively loaded by a open circuited microstrip line has been used previously to produce circular polarization and also as a building block for a series fed microstrip patch array. Rectangular and circular patch antennas loaded with a microstrip stub were previously analyzed using the generalized Thevenin theorem. In the Thevenin theorem approach, the mutual coupling between the patch current and the surface current on the stub was not taken into account. Also, the Thevenin theorem approach neglects continuity of current at the patch-stub junction. The approach in this present paper includes the coupling between the patch and stub currents as well as continuity at the patch-stub junction. The input impedance for a stub loaded microstrip patch is calculated by the general planar dielectric dyadic Green's function approach in the spectral domain, as was initiated much earlier and has been extensively expanded upon and utilized successfully throughout the literature for microstrip antenna configurations. Using the spectral domain dyadic Green s function derived earlier with the electric field integral equation (EFIE), the problem is formulated by using entire domain basis functions to represent the surface current densities on the patch, the loading stub and the attachment mode at the junction. Galerkin's procedure is used to reduce the EFIE to a matrix equation, which is then solved to obtain the amplitudes of the surface currents. These surface currents are then used for calculating the input impedance of stub loaded rectangular and circular microstrip patches. Numerical results are compared with measured results and with previous results calculated by the Thevenin's theorem approach.

Deshpande, M. D.

Reconfigurable Wideband Circularly Polarized Stacked Square Patch Antenna for Cognitive Radios

An almost square patch, a square patch and a stacked square patch with corner truncation for circular polarization (CP) are researched and developed at X-band for cognitive radios. Experimental results indicate, first, that the impedance bandwidth of a CP almost square patch fed from the edge by a 50 ohm line is 1.70% and second, that of a CP square patch fed from the ground plane side by a surface launch connector is 1.87%. Third, the impedance bandwidth of a CP stacked square patch fed by a surface launch connector is 2.22%. The measured center frequency for the CP square patch fed by a surface launch connector without and with an identical stacked patch is 8.45 and 8.1017 GHz, respectively. By stacking a patch, separated by a fixed air gap of 0.254 mm, the center frequency is observed to shift by as much as 348.3 MHz. The shift in center frequency, brought about by the reconfiguring of the physical layer antenna, can be exploited in a cognitive system since it expands the usable frequency spectrum for software reconfiguration in the presence of interference. In addition, varying the fixed air gap in the stacked antenna geometry by increments of 0.254 mm further expands the usable frequency spectrum.

Cognitive

Patches in the polar ionosphere: UT and seasonal dependence

The seasonal and Universal Time (UT) dependencies of patches in the polar ionosphere are simulated using the Utah State University time dependent ionospheric model (TDIM). Patch formation is achieved by changing the plasma convection pattern in response to temporal changes in the interplanetary magnetic field (IMF) B(sub y) component during periods of southwrd IMF. This mechanism redirects the plasma flow from the dayside high-density region, which is the source of the tongue of ionization (TOI) density feature, through the throat and leads to patches, rather than a continuous TOI. The model predicts that the patches are absent at winter solstice (northern hemisphere) between 0800 and 1200 UT and that they have their largest seasonal intensity at winter solstice between 2000 and 2400 UT. Between winter solstice and equinox, patches are strong and present all day. Patches are present in summer as well, although their intensity is only tens of percent above the background density. These winter-to-equinox findings are also shown to be consistent with observations. The model was also used to predict times at which patch observations could be performed to determine the contributions from other patch mechanisms. This observational window is +/- 20 days about winter solstice between 0800 and 1200 UT in the northern hemisphere. In this observational window the TOI is either absent or reduced to a very low density. Hence the time dependent electric field mechanism considered in this study does not produce patches, and if they are observed, then they must be due to some other mechanism.

Sojka, J. J.

An Optimal Cell Detection Technique for Automated Patch Clamping

While there are several hardware techniques for the automated patch clamping of cells that describe the equipment apparatus used for patch clamping, very few explain the science behind the actual technique of locating the ideal cell for a patch clamping procedure. We present a machine vision approach to patch clamping cell selection by developing an intelligent algorithm technique that gives the user the ability to determine the good cell to patch clamp in an image within one second. This technique will aid the user in determining the best candidates for patch clamping and will ultimately save time, increase efficiency and reduce cost. The ultimate goal is to combine intelligent processing with instrumentation and controls in order to produce a complete turnkey automated patch clamping system capable of accurately and reliably patch clamping cells with a minimum amount of human intervention. We present a unique technique that identifies good patch clamping cell candidates based on feature metrics of a cell's (x, y) position, major axis length, minor axis length, area, elongation, roundness, smoothness, angle of orientation, thinness and whether or not the cell is only particularly in the field of view. A patent is pending for this research.

McDowell, Mark

Patches for Repairing Ceramics and Ceramic-Matrix Composites

Patches consisting mostly of ceramic fabrics impregnated with partially cured polymers and ceramic particles are being developed as means of repairing ceramics and ceramic-matrix composites (CMCs) that must withstand temperatures above the melting points of refractory metal alloys. These patches were conceived for use by space-suited, space-walking astronauts in repairing damaged space-shuttle leading edges: as such, these patches could be applied in the field, in relatively simple procedures, and with minimal requirements for specialized tools. These design characteristics also make the patches useful for repairing ceramics and CMCs in terrestrial settings. In a typical patch as supplied to an astronaut or repair technician, the polymer would be in a tacky condition, denoted as an A stage, produced by partial polymerization of a monomeric liquid. The patch would be pressed against the ceramic or CMC object to be repaired, relying on the tackiness for temporary adhesion. The patch would then be bonded to the workpiece and cured by using a portable device to heat the polymer to a curing temperature above ambient temperature but well below the maximum operating temperature to which the workpiece is expected to be exposed. The patch would subsequently become pyrolized to a ceramic/glass condition upon initial exposure to the high operating temperature. In the original space-shuttle application, this exposure would be Earth-atmosphere-reentry heating to about 3,000 F (about 1,600 C). Patch formulations for space-shuttle applications include SiC and ZrO2 fabrics, a commercial SiC-based pre-ceramic polymer, and suitable proportions of both SiC and ZrO2 particles having sizes of the order of 1 m. These formulations have been tailored for the space-shuttle leading-edge material, atmospheric composition, and reentry temperature profile so as to enable repairs to survive re-entry heating with expected margin. Other formulations could be tailored for specific terrestrial applications.

Hogenson, Peter A.

Single-Layer, All-Metal Patch Antenna Element with Wide Bandwidth

It is known that the impedance at the center of a patch antenna element is a short circuit, implying that a wire or post can be connected from the patch to the groundplane at this point without impacting radiation performance. In principle, this central post can be used to support the patch element, thus eliminating the need for dielectric. In spaceborne applications, this approach is problematic because a patch element supported by a single, thin post is highly susceptible to acoustic loads during launch. The technology reported here uses a large-diameter center post as its supporting structure. The supporting structure allows for the fabrication of a sufficiently rigid antenna element that can survive launch loads. The post may be either hollow or solid, depending on fabrication approach and/or mass constraints. The patch antenna element and support post are envisioned as being fabricated (milled) from a single piece of aluminum or other metal. Alternately, the patch plate and support column can be fabricated separately and then joined using fasteners, adhesive, or welding. Casting and electroforming are also viable techniques for manufacturing the metal patch part(s). The patch structure is then either bonded or fastened to the supporting groundplane. Arrays of patch elements can be fabricated by attaching several structures to a common groundplane/support structure.

Chamberlain, Neil F.

Effect of 3D Roughness Patch on Instability Amplification in a Supersonic Boundary Layer

Surface roughness is known to have a substantial impact on the aerothermodynamic loading of high-speed vehicles, particularly via its influence on the laminar-turbulent transition process within the boundary layer. Numerical simulations are performed to investigate the effects of a distributed region of densely packed, sinusoidal shape roughness elements on a Mach 3.5 flat plate boundary layer for flow conditions corresponding to the planned conditions of an upcoming experiment in the Mach 3.5 Supersonic Low Disturbance Tunnel at the NASA Langley Research Center. Analysis of convective instabilities in the wake of the roughness patch was reported in a previous paper and the current work extends that analysis to instability amplification across the length of the roughness patch. Quasi-parallel stability analysis of the modified boundary layer flow over the patch indicates two dominant families of unstable disturbances, namely, a group of high frequency modes that amplify in localized regions along the roughness patch and another group of low frequency modes that have smaller peak amplification rates but amplify steadily both above the roughness patch and in the wake region behind it. The results suggest that the amplification factors associated with the high-frequency modes are sufficiently low, at least for the roughness patches considered in this paper, so that these modes are unlikely to have a major influence on the transition process. The amplification of the low-frequency modes within the region of the roughness patch is further quantified via direct numerical simulations. Results confirm the strongly destabilizing influence of the roughness patch on the first mode instabilities, yielding an N-factor increment of ΔN ≈ 3.6 for a roughness patch length of eight wavelengths.

Laminar turbulent transition

Integration of an Arm Kinematics Hot Patch onboard the Curiosity Rover

NASA's Mars Science Laboratory (MSL) mission has updated the Curiosity rover's flight software multiple times since landing on Mars on August 6, 2012. The most common patching method has been a hot patch, in which running flight software is modified after being copied into RAM from its persistent storage. The latest hot patch to be installed on Curiosity fixed an issue in the robotic arm software that computes generalized inverse kinematics. Additional unit testing performed since the start of the surface mission revealed that this software can sometimes produce erroneous solutions.The cause was identified as numerical instability in a quartic root finder. When the inputs to that solver are not well conditioned, floating-point numerical issuescan cause erroneous roots to be reported. In theory, this could result in the robotic arm turret instruments being commanded to unintended positions, for example, below the terrain surface. Out of approximately 3.7 million unit test cases, 97.2\% of the position errors were below 5 mm. However, there were 16 test cases where theposition error was greater than 20 cm, and the maximum position error was 1.2 meters.The patch was uploaded to Curiosity on sol 2642 (January 11, 2020) after the solution was developed, re-implemented as a hot patch, and validated and verified using Earth-based Curiosity testbeds. A checkout test of the patch was performed on Curiosity on sol 2657, and nominal use of the patch began on sol 2658. In this paper, we describe the steps that led to integrating the arm kinematic hot patch into Curiosity's flight software, from the discovery of the bug to the nominal use of the patch in flight.

Maimone, Mark

Triangular Berstein-bezier Patches, a Survey and New Results

Triangular Bernstein-Bezier patches are an alternative to the standard rectangular ones. They are maps of triangular domains into R cubed, where the patches are described by control nets similar to the rectangular case. One difference in the definition of surface normals at the patch corners: rectangular patches have inherent problems with so-called degenerate corners, i.e., edges collapsing into one point. Triangular patches do not have these problems. Since triangular shapes arise rather frequently in the design of complicated surfaces (e.g., interior car body panels), it seems that for such surfaces triangular patches are a promising addition to the usually employed rectangular patches. Another major application is the formulation of interpolants to solve the scattered data problem: here Bernstein-Bezier patches provide a very powerful tool; they can be used to discover new schemes as well as to discover new properties of existing methods.

Farin, G.

Metal Patch Antenna

Disclosed herein is a patch antenna comprises a planar conductive patch attached to a ground plane by a support member, and a probe connector in electrical communication with the conductive patch arranged to conduct electromagnetic energy to or from the conductive patch, wherein the conductive patch is disposed essentially parallel to the ground plane and is separated from the ground plane by a spacing distance; wherein the support member comprises a plurality of sides disposed about a central axis oriented perpendicular to the conductive patch and the ground plane; wherein the conductive patch is solely supported above the ground plane by the support member; and wherein the support member provides electrical communication between the planer conductive patch and the ground plane.

Chamberlain, Neil F.

Patch-level chamber-based CH4 and CO2 flux measurements in freshwater and salt marshes of coastal Louisiana

This dataset contains chamber-based carbon dioxide and methane flux measurements from ecohydrological patches in two coastal wetlands in Louisiana (within the footprint of AmeriFlux sites US-LA2 and US-LA3). Measurements span four patches, two per wetland. At the freshwater marsh (US-LA2), patches were dominated by Sagittaria lancifolia or co-dominated by Sagittaria lancifolia and Typha latifolia. At the salt marsh site (US-LA3), patches were dominated by Spartina alterniflora or Juncus roemerianus. Flux measurements included (i) wetland surface-level measurements encompassing the entire soil-water-vegetation column and (ii) soil-water surface chamber measurements that excluded emergent vegetation. At US-LA3, open-water pools were common and were also measured as a patch type. With this data we aimed to assess differences in gas flux pathways within ecohydrological patches across different wetland types. Files can be read in any program or code typical of processing .csv file types. "PatchChambers_LA2LA3.csv" covers flux data from patch level chambers which includes bulk CO2/CH4 flux encompassing water, soil, and vegetation within each chamber. "Water_Soil_SurfaceChamber_LA2LA3.csv" has CO2/CH4 flux data from chambers measuring only the water-soil surface without the effects of vegetation. Metadata can be found in the similarly named files for each data sheet ("xxxx_md.csv").

54 ENVIRONMENTAL SCIENCES

The modeling of piezoceramic patch interactions with shells, plates and beams

General models describing the interactions between a pair of piezoceramic patches and elastic substructures consisting of a cylindrical shell, plate and beam are presented. In each case, the manner in which the patch loads enter both the strong and weak forms of the time-dependent structural equations of motion is described. Through force and moment balancing, these loads are then determined in terms of material properties of the patch and substructure (thickness, elastic properties, Poisson ratios), the geometry of the patch placement, and the voltages into the patches. In the case of the shell, the coupling between banding and inplane deformations, which is due to the curvature, is retained. These models are sufficiently general to allow for potentially different patch voltages which implies that they can be suitably employed when using piezoceramic patches for controlling system dynamics when both extensional and bending vibrations are present.

Banks, H. T.

Structures in Ionospheric Number Density and Velocity Associated with Polar Cap Ionization Patches

Spectral characteristics of polar cap F region irregularities on large density gradients associated with polar ionization patches are studied using in situ measurements made by the Dynamics Explorer 2 (DE 2) spacecraft. The 18 patches studied in this paper were identified by the algorithm introduced by Coley and Heelis, and they were encountered during midnight-noon passes of the spacecraft. Density and velocity spectra associated with these antisunward convecting patches are analyzed in detail. Observations indicate the presence of structure on most patches regardless of the distance between the patch and the cusp where they are believed to develop. Existence of structure on both leading and trailing edges is established when such edges exist. Results, which show no large dependence of Delta N/N power on the sign of the edge gradient del N, do not allow the identification of leading and trailing edges of the patch. The Delta N/N is an increasing function of gradient del N regardless of the sign of the gradient. The correlation between Delta N/N and Delta V is generally poor, but for a given intensity in Delta V, Delta N/N maximizes in regions of large gradients in N. There is evidence for the presence of unstructured patches that seem to co-exist with unstructured horizontal velocities. Slightly smaller spectral indices for trailing edges support the presence of the E X B drift instability. Although this instability is found to be operating in some cases, results suggest that stirring may be a significant contributor to kilometer-size structures in the polar cap.

Kivanc, O.

Analysis of Stub Loaded Microstrip Patch Antennas

A microstrip patch antenna fed by a coaxial probe and reactively loaded by a open circuited microstrip line has been used previously to produce circular polarization[ l] and also as a building block for a series fed microstrip patch array [2]. Rectangular and circular patch antennas loaded with a microstrip stub were previously analyzed using the generalized Thevenin theorem [2,3]. In the Thevenin theorem approach, the mutual coupling between the patch current and the surface current on the stub was not taken into account. Also, the Thevenin theorem approach neglects continuity of current at the patch-stub junction. The approach in this present paper includes the coupling between the patch and stub currents as well as continuity at the patch-stub junction.

Bailey, M. C.