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Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

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At least 19 records
All sourcesNASA NTRSDOE OSTI
NASA NTRS1998

Nitrogen balance for wheat canopies (Triticum aestivum cv. Veery 10) grown under elevated and ambient CO2 concentrations

We examined the hypothesis that elevated CO2 concentration would increase NO3- absorption and assimilation using intact wheat canopies (Triticum aestivum cv. Veery 10). Nitrate consumption, the sum of plant absorption and nitrogen loss, was continuously monitored for 23 d following germination under two CO2 concentrations (360 and 1000 micromol mol-1 CO2) and two root zone NO3- concentrations (100 and 1000 mmol m3 NO3-). The plants were grown at high density (1780 m-2) in a 28 m3 controlled environment chamber using solution culture techniques. Wheat responded to 1000 micromol mol-1 CO2 by increasing carbon allocation to root biomass production. Elevated CO2 also increased root zone NO3- consumption, but most of this increase did not result in higher biomass nitrogen. Rather, nitrogen loss accounted for the greatest part of the difference in NO3- consumption between the elevated and ambient [CO2] treatments. The total amount of NO3(-)-N absorbed by roots or the amount of NO3(-)-N assimilated per unit area did not significantly differ between elevated and ambient [CO2] treatments. Instead, specific leaf organic nitrogen content declined, and NO3- accumulated in canopies growing under 1000 micromol mol-1 CO2. Our results indicated that 1000 micromol mol-1 CO2 diminished NO3- assimilation. If NO3- assimilation were impaired by high [CO2], then this offers an explanation for why organic nitrogen contents are often observed to decline in elevated [CO2] environments.

Non-NASA Center↗
NASA NTRS1994

Effect of land use change on the carbon cycle in Amazon soils

The overall goal of this study was to provide a quantitative understanding of the cycling of carbon in the soils associated with deep-rooting Amazon forests. In particular, we wished to apply the understanding gained by answering two questions: (1) what changes will accompany the major land use change in this region, the conversion of forest to pasture? and (2) what is the role of carbon stored deeper than one meter in depth in these soils? To construct carbon budgets for pasture and forest soils we combined the following: measurements of carbon stocks in above-ground vegetation, root biomass, detritus, and soil organic matter; rates of carbon inputs to soil and detrital layers using litterfall collection and sequential coring to estimate fine root turnover; C-14 analyses of fractionated SOM and soil CO2 to estimate residence times; C-13 analyses to estimate C inputs to pasture soils from C-4 grasses; soil pCO2, volumetric water content, and radon gradients to estimate CO2 production as a function of soil depth; soil respiration to estimate total C outputs; and a model of soil C dynamics that defines SOM fractions cycling on annual, decadal, and millennial time scales.

Trumbore, Susan E.↗
NASA NTRS1994

Completing below-ground carbon budgets for pastures, recovering forests, and mature forests of Amazonia

The objective of this grant was to complete below-ground carbon budgets for pastures and forest soils in the Amazon. Profiles of radon and carbon dioxide were used to estimate depth distribution of CO2 production in soil. This information is necessary for determining the importance of deep roots as sources of carbon inputs. Samples were collected for measuring root biomass from new research sites at Santana de Araguaia and Trombetas. Soil gases will be analyzed for CO2 and (14)CO2, and soil organic matter will be analyzed for C-14. Estimates of soil texture from the RADAMBRASIL database were merged with climate data to calculate soil water extraction by forest canopies during the dry season. In addition, a preliminary map of areas where deep roots are needed for deep soil water was produced. A list of manuscripts and papers prepared during the reporting periods is given.

Davidson, Eric A.↗
NASA NTRS1990

Seismic stress responses of soybean to different photosynthetic photon flux

Physical agitation applied as periodic seismic stress (shaking) reduced stem elongation, leaf expansion, and biomass accumulation by vegetative soybeans. Level of photon flux (PPF) influenced the type and extent of plant response to mechanical stress. Plant parts responded differently as PPF varied between 135 and 592 micromoles m-2 s-1. Stem length was significantly reduced by seismic stress at 135 micromoles m-2 s-1 but this effect was insignificant at higher PPFs. Reduced stem length resulted from an inhibition of internode elongation. Stem diameter was unaffected by stress at the PPFs tested. In contrast to effects on stem elongation, leaf area was insensitive to stress treatments at 135 micromoles m-2 s-1 but was progressively inhibited by stress as PPF increased. Statistically significant reductions in shoot f. wt and d. wt by seismic stress occurred only at 295 micromoles m-2 s-1. Root biomass accumulation was not affected by seismic stress at any PPF used in this study.

NASA Program Space Biology↗
NASA NTRS2008

Model Effects on GLAS-Based Regional Estimates of Forest Biomass and Carbon

ICESat/GLAS waveform data are used to estimate biomass and carbon on a 1.27 million sq km study area. the Province of Quebec, Canada, below treeline. The same input data sets and sampling design are used in conjunction with four different predictive models to estimate total aboveground dry forest biomass and forest carbon. The four models include nonstratified and stratified versions of a multiple linear model where either biomass or (square root of) biomass serves as the dependent variable. The use of different models in Quebec introduces differences in Provincial biomass estimates of up to 0.35 Gt (range 4.942+/-0.28 Gt to 5.29+/-0.36 Gt). The results suggest that if different predictive models are used to estimate regional carbon stocks in different epochs, e.g., y2005, y2015, one might mistakenly infer an apparent aboveground carbon "change" of, in this case, 0.18 Gt, or approximately 7% of the aboveground carbon in Quebec, due solely to the use of different predictive models. These findings argue for model consistency in future, LiDAR-based carbon monitoring programs. Regional biomass estimates from the four GLAS models are compared to ground estimates derived from an extensive network of 16,814 ground plots located in southern Quebec. Stratified models proved to be more accurate and precise than either of the two nonstratified models tested.

Nelson, Ross↗
NASA NTRS1979

Red and photographic infrared linear combinations for monitoring vegetation

The relationships between various linear combinations of red and photographic infrared radiances and vegetation parameters are investigated. In situ spectrometers are used to measure the relationships between linear combinations of red and IR radiances, their ratios and square roots, and biomass, leaf water content and chlorophyll content of a grass canopy in June, September and October. Regression analysis shows red-IR combinations to be more significant than green-red combinations. The IR/red ratio, the square root of the IR/red ratio, the vegetation index (IR-red difference divided by their sum) and the transformed vegetation index (the square root of the vegetation index + 0.5) are found to be sensitive to the amount of photosynthetically active vegetation. The accumulation of dead vegetation over the year is found to have a linearizing effect on the various vegetation measures.

Tucker, C. J.↗
NASA NTRS2010

Root Zone Respiration on Hydroponically Grown Wheat Plant Systems

Root respiration is a biological phenomenon that controls plant growth and physiological development during a plant's lifespan. This process is dependent on the availability of oxygen in the system where the plant is located. In hydroponic systems, where plants are submerged in a solution containing vital nutrients but no type of soil, the availability of oxygen arises from the dissolved oxygen concentration in the solution. This oxygen concentration is dependent on the , gas-liquid interface formed on the upper surface of the liquid, as given by Henry's Law, depending on pressure and temperature conditions. Respiration rates of the plants rise as biomass and root zone increase with age. The respiration rate of Apogee wheat plants (Triticum aestivum) was measured as a function of light intensity (catalytic for photosynthesis) and CO2 concentration to determine their effect on respiration rates. To determine their effects on respiration rate and plant growth microbial communities were introduced into the system, by Innoculum. Surfactants were introduced, simulating gray-water usage in space, as another factor to determine their effect on chemical oxygen demand of microbials and on respiration rates of the plants. It is expected to see small effects from changes in CO2 concentration or light levels, and to see root respiration decrease in an exponential manner with plant age and microbial activity.

Soler-Crespo, R. A.↗
NASA NTRS2019

How Does Water Delivery System Design Impact the Microbial Load of Salad Crops?

In a microgravity setting, such as the environment aboard the International Space Station (ISS), an ideal plant water delivery system is one that can grow edible crops with minimal resource consumption and minimal risk to crew members. There are also concerns associated with the ability to control fluid escape and biofilm formation resulting in potential dangers to systems, crops, or crewmembers. To identify an appropriate system, candidate systems were assembled and operated under simulated ISS environmental conditions (T,CO2,and RH) with red romaine lettuce (Lactuca sativa cultivar 'Outredgeous') as a model crop. Fluid reservoirs and randomly selected planting sites were sampled every seven days until maturity at which point edible plant biomass and root samples were also taken. Heterotrophic bacteria and fungi growth patterns throughout each planting cycle were determined by plate counts on appropriate agar media. The candidate systems were compared to a classic hydroponics system as a control and harvested crops were compared to controls as well as Veggie-grown and market produce. Plants harvested from candidate systems yielded lower average heterotrophic bacteria and fungi per gram of plant mass levels when compared to market and Veggie samples as well as those from the control system. Additional studies to evaluate the system sanitation regimen as well as testing additional crops should be considered to aid in the selection of an ideal system.

Curry, Aaron B.↗
NASA NTRS1995

On the use of antibiotics to reduce rhizoplane microbial populations in root physiology and ecology investigations

No straightforward method exists for separating the proportion of ion exchange and respiration due to rhizoplane microbial organisms from that of root ion exchange and respiration. We examined several antibiotics that might be used for the temporary elimination of rhizoplane bacteria from hydroponically grown wheat roots (Triticum aestivum cv. Veery 10). Each antibiotic was tested for herbicidal activity and plate counts were used to enumerate bacteria and evaluate antibiotic kinetics. Only lactam antibiotics (penicillins and cephalosporins) did not reduce wheat growth rates. Aminoglycosides, the pyrimidine trimethoprim, colistin and rifampicin reduced growth rates substantially. Antibiotics acted slowly, with maximum reductions in rhizoplane bacteria occurring after more than 48 h of exposure. Combinations of nonphytotoxic antibiotics reduced platable rhizoplane bacteria by as much as 98%; however, this was generally a reduction from about 10(9) to 10(6) colony forming units per gram of dry root mass, so that many viable bacteria remained on root surfaces. We present evidence which suggests that insufficient bacterial biomass exists on root surfaces of nonstressed plants grown under well-aerated conditions to quantitatively interfere with root nitrogen absorption measurements.

NASA Discipline Life Support Systems↗
NASA NTRS2017

Development of a Coherent Bistatic Vegetation Model for Signal of Opportunity Applications at VHF UHF-Bands

A coherent bistatic vegetation scattering model, based on a Monte Carlo simulation, is being developed to simulate polarimetric bi-static reflectometry at VHF/UHF-bands (240-270 MHz). The model is aimed to assess the value of geostationary satellite signals of opportunity to enable estimation of the Earth's biomass and root-zone soil moisture. An expression for bistatic scattering from a vegetation canopy is derived for the practical case of a ground-based/low altitude platforms with passive receivers overlooking vegetation. Using analytical wave theory in conjunction with distorted Born approximation (DBA), the transmit and receive antennas effects (i.e., polarization, orientation, height, etc.) are explicitly accounted for. Both the coherency nature of the model (joint phase and amplitude information) and the explicit account of system parameters (antenna, altitude, polarization, etc) enable one to perform various beamforming techniques to evaluate realistic deployment configurations. In this paper, several test scenarios will be presented and the results will be evaluated for feasibility for future biomass and root-zone soil moisture application using geostationary communication satellite signals of opportunity at low frequencies.

UFH↗
NASA NTRS2022

Microgreens Root Zone/Shoot Zone Partitioned Planting Box

To enable sustainable food production in future human exploration missions, plant growth is being studied by the Space Crop Production Team at KSC. Microgreens are good candidates for food supplements and contain specific nutrients that are lacking in the prepackaged diet, including vitamin C and vitamin K. Because they are densely sown, typical growth methods do not allow the ability to distinguish between the levels of evaporation from the rooting substrate and transpiration from the leaves. With larger plants, the root and shoot zone can be separated to distinguish these fluxes and accurate transpiration measurements of plant canopies are feasible. Furthermore, separation of the root and shoot zone may also be beneficial when harvesting microgreens in microgravity as it may reduce microbial contamination of the edible biomass by the roots, which have high microbial loads. Using a root and shoot separator box when harvesting may help with microbial contamination, but these tiny plants are challenging to handle in microgravity, so harvest management remains an open question. The innovation proposed here is a microgreen root/shoot partitioned planting box, which offers a solution to these challenges with accurate gas exchange measurements and a safe microgreen harvest in low gravity environments. Being able to measure transpiration of a microgreen canopy will be important for modeling plant growth in reduced gravity environments, so the first objective was to develop a planting unit with a seal between the root zone and the shoot zone. With an unsuitable harvesting technique, freshly harvested microgreens may add debris to the cabin, so the second objective was to test different harvesting techniques and management approaches associated with this innovation. These two objectives were pursued in parallel since many goals were the same: develop a planting unit that 1) separates the shoots from the roots, 2) allows acceptable germination rate, and 3) allows for seedlings to emerge and develop. What differed was the need to have a seal, which was only applicable for our gas exchange goal, and the need to have an embedded harvesting mechanism and bagging method which only applied to our harvesting goal. Testing of the various harvesting mechanisms and bagging methods was performed during a series of parabolic flights. All parabolic flight procedures took place inside a rented secondary containment chamber (e.g., glovebox) that was developed by the University of Louisville specifically for experiments involving fluids and other materials that may become airborne during reduced gravity flight. Three different harvesting methods and two different bagging collection methods were tested for microgreens. A third bagging method was initially tested but found to be unsuitable. Human factors were also taken into consideration, to identify which harvest and bagging collection methods would be easiest to use with favorable results in microgravity. Three parabolic flight tests were performed in total, one in November 2021 and two in December 2021.

Gioia Massa↗
NASA NTRS1993

Statistical analysis of environmental variability within the CELSS breadboard project's biomass production chamber

Variability in the aerial and root environments of NASA's Breadboard Project's Biomass Production Chamber (BPC) was determined. Data from two lettuce and two potato growouts were utilized. One growout of each crop was conducted prior to separating the upper and lower chambers; the other was subsequent to separation. There were little or no differences in pH, EC, or solution temperature between the upper and lower chamber or within a chamber. Variation in the aerial environment within a chamber was two to three times greater than variation between chambers for air temperature, relative humidity, and PPF. High variability in air velocity, relative to tray position, was observed. Separating the BPC had no effect on PPF, air velocity, solution temperature, pH, or EC. Separation reduced the gradient in air temperature and relative humidity between the upper and lower chambers, but increased the variability within a chamber. Variation between upper and lower chambers was within 5 percent of environmental set-points and of little or no physiological significance. In contrast, the variability within a chamber limits the capability of the BPC to generate statistically reliable data from individual tray treatments at this time.

Stutte, G. W.↗
NASA NTRS1989

Sweet potato growth parameters, yield components and nutritive value for CELSS applications

Sweet potatoes have been grown hydroponically using the nutrient film technique (NFT) to provide a potential food source for long-term manned space missions. Experiments in both sand and NFT cultivars have produced up to 1790 g/plant of fresh storage root with an edible biomass index ranging from 60-89 percent and edible biomass linear growth rates of 39-66 g/sq m day in 105 to 130 days. Experiments with different cultivars, nutrient solution compositions, application rates, air and root temperatures, photoperiods, and light intensities indicate good potential for sweet potatoes in CELSS.

Loretan, P. A.↗
NASA NTRS2012

A Parameterized Inversion Model for Soil Moisture and Biomass from Polarimetric Backscattering Coefficients

A semi-empirical algorithm for the retrieval of soil moisture, root mean square (RMS) height and biomass from polarimetric SAR data is explained and analyzed in this paper. The algorithm is a simplification of the distorted Born model. It takes into account the physical scattering phenomenon and has three major components: volume, double-bounce and surface. This simplified model uses the three backscattering coefficients ( sigma HH, sigma HV and sigma vv) at low-frequency (P-band). The inversion process uses the Levenberg-Marquardt non-linear least-squares method to estimate the structural parameters. The estimation process is entirely explained in this paper, from initialization of the unknowns to retrievals. A sensitivity analysis is also done where the initial values in the inversion process are varying randomly. The results show that the inversion process is not really sensitive to initial values and a major part of the retrievals has a root-mean-square error lower than 5% for soil moisture, 24 Mg/ha for biomass and 0.49 cm for roughness, considering a soil moisture of 40%, roughness equal to 3cm and biomass varying from 0 to 500 Mg/ha with a mean of 161 Mg/ha

soil moisture↗
NASA NTRS2002

Effects of Disinfectants in Water on Mir- and Earth-Grown Wheat

Iodine and silver fluoride are used to purify water onboard U. S. Shuttles and the Russian Space Station, Mir, respectively. In 1995, iodine-treated water, which ranged from 1.0-4.0 mg x kg(exp -1) with a mean of 2.9 mg x kg(exp -1), was applied to Super Dwarf wheat (Triticum aestivum L.) plants when Mir water (grey or tech grade) became scarce. The potential phytotoxicity of iodine on Super Dwarf wheat is an unknown. Since use of iodine-treated water was not part of the experiment, we sought to determine whether it accounted for the subsequent poor wheat seedling growth and floral development onboard the Mir. Super Dwarf wheat seeds were imbibed in iodine or silver fluoride concentrations of 0.0, 1.0, 2.0, 4.0, 8.0 or 16.0 mg x kg(exp -1) for 96 h at 4 C. Five seeds were then planted per 13.3 cm x 13.3 cm pots containing a granular clinoptilolite (Cp) zeolite (1 -2 mm dia.) and placed in Percival(TM) growth chambers programmed for 20/15 C and 18/6 h d/n regime. Plants were irrigated with distilled water, and Iodine- or silver fluoride-treated distilled water. In separate experiments, seeds were treated as above and germination and early seedling growth were determined by examining seedling responses to disinfectants in rolled paper towels. Silver fluoride had very little effect on wheat seed germination. By contrast, iodine reduced germination at all treatment levels. Seedlings exposed to 1.0, 2.0, and 4.0 mg x kg(exp -1) of iodine or silver fluoride levels exhibited a slight stimulation in shoot and root growth. Both disinfectants at 8 and 16 mg x kg(exp -1) showed significantly (p is less than or equal to 0.01) reduced seedling shoot and root lengths and fresh biomasses compared to the control and lower disinfectant levels. The number of spikelets per spike, florets per spikelet, seeds per spike and seed weight were also significantly reduced at the 8 and 16 mg x kg(exp -1) compared to the control and lower levels of disinfectant. Based on these ground-based post-flight analyses, the levels of iodine- and/or silver fluoride-treated water used on Mir-grown plants onboard the Mir did not cause the poor growth and development of the wheat plants.

Campbell, William .F.↗
NASA NTRS2021

Synthesizing Disparate LiDAR and Satellite Datasets through Deep Learning to Generate Wall-to-Wall Regional Inventories for the Complex, Mixed-Species Forests of the Eastern United States

Light detection and ranging (LiDAR) has become a commonly-used tool for generating remotely-sensed forest inventories. However, LiDAR-derived forest inventories have remained uncommon at a regional scale due to varying parameters among LiDAR data acquisitions and the availability of sufficient calibration data. Here, we present a model using a 3-D convolutional neural network (CNN), a form of deep learning capable of scanning a LiDAR point cloud, combined with coincident satellite data (spectral, phenology, and disturbance history). We compared this approach to traditional modeling used for making forest predictions from LiDAR data (height metrics and random forest) and found that the CNN had consistently lower uncertainty. We then applied the CNN to public data over six New England states in the USA, generating maps of 14 forest attributes at a 10 m resolution over 85% of the region. Aboveground biomass estimates produced a root mean square error of 36 Mg ha−1 (44%) and were within the 97.5% confidence of independent county-level estimates for 33 of 38 or 86.8% of the counties examined. CNN predictions for stem density and percentage of conifer attributes were moderately successful, while predictions for detailed species groupings were less successful. The approach shows promise for improving the prediction of forest attributes from regional LiDAR data and for combining disparate LiDAR datasets into a common framework for large-scale estimation.

Elias Ayrey↗
NASA NTRS1996

Biomass accumulation in hydroponically grown sweetpotato in a controlled environment: a preliminary study

In the development of a plant growth model, the assumptions made and the general equations representing an understanding of plant growth are gradually refined as more information is acquired through experimentation. One such experiment that contributed to sweetpotato model development consisted of measuring biomass accumulation of sweetpotato grown in hydroponic culture in a plant growth chamber. Plants were started from fifteen centimeter long 'TU-82-155' sweetpotato vine cuttings spaced 25 cm apart in each of 18 rectangular growing channels (0.15 by 0.15 by 1.2m) in a system designed to use the nutrient film technique (NFT). Each channel contained four plants. The 3.5m by 5.2m plant growth chamber environmental parameters included an 18h photoperiod, 500 micromoles m-2 s-1 of photosynthetic photon flux (PPF), and a diurnal light/dark temperature of 28 degrees C/22 degrees C. The relative humidity was 80 +/- 5% and the CO2 partial pressure was ambient (350 ppm). The nutrient solution contained in 30L reservoirs was a modified half Hoagland's solution with a 1:2.4 N:K ratio and a pH of 6.2. Solution replenishment occurred when the electrical conductivity (EC) level dropped below 1050. Plants were harvested at 15 days after planting (DAP) and weekly thereafter until day 134. By 57 DAP, stems and fibrous roots had acquired 90% of their total dry biomass, while leaves had reached 84% of their maximum dry biomass. Beginning at 64 DAP dry biomass accumulation in the storage roots dominated the increase in dry biomass for the plants. Dry weight of storage roots at 120 DAP was 165 g/plant or 1.1 kg/m2. Resulting growth curves were consistent with the physiological processes occurring in the plant. Results from this study will be incorporated in a plant growth model for use in conjunction with controlled life support systems for long-term manned space missions.

Biomass↗
NASA NTRS1988

Hormonal regulation of wheat growth during hydroponic culture

Hormonal control of root growth has been explored as one means to alleviate the crowding of plant root systems experienced in prototype hydroponic biomass production chambers being developed by the CELSS Breadboard Project. Four plant hormones, or their chemical analogs, which have been reported to selectively inhibit root growth, were tested by adding them to the nutrient solutions on day 10 of a 25 day growth test using spring wheat in hydroponic cultures. Growth and morphological changes is both shoot and root systems were evaluated. In no case was it possible to inhibit root growth without a comparable inhibition of shoot growth. It was concluded that this approach is unlikely to prove useful for wheat.

Wetherell, Donald↗