Search NASA⌕ Search

Engineering topics

Cook, Ryan

Publications and source records attributed to Cook, Ryan.

4 records
All sourcesNASA NTRSDOE OSTI
DOE OSTI2025

Engineering a protease-stable, oral single-domain antibody to inhibit IL-23 signaling

Interleukin (IL)-23 is a validated therapeutic target in inflammatory bowel disease. While antibodies targeting IL23 demonstrate clinical efficacy, they face challenges such as high costs, safety risks, and the necessity of parenteral administration. Here, we present a workflow to simultaneously enhance the affinity and protease stability of an inhibitory anti-IL23R VHH for oral use. Cocrystal structure analysis reveals that the anti-IL23R VHH employs both CDR and framework residues to achieve picomolar affinity for IL23R. The engineered VHH remains stable for over 8 h in intestinal fluid and 24 h in fecal samples. Oral administration of this VHH achieves deep pathway inhibition in a murine colitis model. Furthermore, a single pill provides sustained IL23R inhibition in nonhuman primate blood for over 24 h. With high potency, gut stability, high production yield, and favorable drug-like properties, oral VHHs offer a promising approach for inflammatory bowel diseases.

Science & Technology - Other Topics↗
DOE OSTI2023

XCT System for Harvesting In-Current Hydrokinetic Energy from Low-Velocity Sites

The Project demonstrated the functionality of a cross cutting (XCT) type turbine in laboratory and open water testing. The fundamental design approach of locating small axial rotors near the ends of driving spars, which pull the rotors through the water at a multiple of the free-stream velocity eliminates the need (and thus the cost) for a gearbox and torque resisting structures. This configuration, however, introduces the complexity of transmitting electricity across a rotating interface. Even so, cost models indicate that depending on the anchoring and mooring system, LCOE less than $0.10/kWh should be possible in high capacity-factor sites with non-reversing currents.

13 HYDRO ENERGY↗
DOE OSTI2022

Prefabricated Standard Modular Hydropower Installations for Low-cost Small Hydropower

The goal of the present project was to investigate the potential savings in greenfield hydropower project costs across sites having vastly different characteristics. Littoral Power Systems, Inc. (LPS) undertook the project described herein to explore the promise of Standard Modular Hydropower (SMH) to transform the processes of site identification, conceptual design, detailed design, and construction planning to support low- cost, environmentally attractive development of small hydropower facilities at new stream- reaches. This report includes descriptions of the work to develop these processes, the resulting facility designs achieved using these processes and the projected economics. Installation cost estimates for the resulting conceptual greenfield facility design at two different locations come in at $\$3,419$/kW for Balcony Falls and $\$2,124$/kW for LPS 566. The installation time from site preparation to commissioning-ready is estimated to be 58 weeks for the conceptual facility at Balcony Falls and 42 weeks for the conceptual facility at LPS 566. A real challenge to any hydropower facility – not only the two cases studied here – is that environmental and social conditions can change, and as such, the facility may be required to change. The h-Modulor modules connect uniquely and are structurally independent, enabling site configurations to be changed on the fly, as needed. These results show that LPS's h-Modulor SMH approach delivers an attractive balance between efficiency, economics, and environmental sustainability for small hydro greenfield sites.

13 HYDRO ENERGY↗