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Li, Xuan

Publications and source records attributed to Li, Xuan.

At least 19 records
All sourcesNASA NTRSDOE OSTI
DOE OSTI2024

Powdery mildew effectors AVR A1 and BEC1016 target the ER J‐domain protein Hv ERdj3B required for immunity in barley

Abstract The barley powdery mildew fungus, Blumeria hordei (Bh), secretes hundreds of candidate secreted effector proteins (CSEPs) to facilitate pathogen infection and colonization. One of these, CSEP0008, is directly recognized by the barley nucleotide‐binding leucine‐rich‐repeat (NLR) receptor MLA1 and therefore is designated AVR A1 . Here, we show that AVR A1 and the sequence‐unrelated Bh effector BEC1016 (CSEP0491) suppress immunity in barley. We used yeast two‐hybrid next‐generation interaction screens (Y2H‐NGIS), followed by binary Y2H and in planta protein–protein interactions studies, and identified a common barley target of AVR A1 and BEC1016, the endoplasmic reticulum (ER)‐localized J‐domain protein Hv ERdj3B. Silencing of this ER quality control (ERQC) protein increased Bh penetration. Hv ERdj3B is ER luminal, and we showed using split GFP that AVR A1 and BEC1016 translocate into the ER signal peptide‐independently. Overexpression of the two effectors impeded trafficking of a vacuolar marker through the ER; silencing of Hv ERdj3B also exhibited this same cellular phenotype, coinciding with the effectors targeting this ERQC component. Together, these results suggest that the barley innate immunity, preventing Bh entry into epidermal cells, requires ERQC. Here, the J‐domain protein Hv ERdj3B appears to be essential and can be regulated by AVR A1 and BEC1016. Plant disease resistance often occurs upon direct or indirect recognition of pathogen effectors by host NLR receptors. Previous work has shown that AVR A1 is directly recognized in the cytosol by the immune receptor MLA1. We speculate that the AVR A1 J‐domain target being inside the ER, where it is inapproachable by NLRs, has forced the plant to evolve this challenging direct recognition.

54 ENVIRONMENTAL SCIENCES↗
DOE OSTI2023

Advanced silicon tracking detector developments for the future Electron-Ion Collider

The proposed Electron-Ion Collider (EIC) will operate high-luminosity high-energy electron+proton and electron+nucleus collisions at the collision energies from 20 GeV to 141 GeV to solve several fundamental questions in the high energy and nuclear physics fields. Its instantaneous luminosity can reach 10 33-34 cm -2 s -1 and the bunching crossing rate is around 10 ns. The EIC project has received CD1 approval from the US DOE in 2021 and moves toward the machine design and preparation for construction. To realize various particle measurements with high precision at the future EIC, a low material-budget and high-granularity silicon vertex and tracking detector with fine spatial and momentum resolutions and nearly 4π solid angle coverage is desired. The Monolithic Active Pixel Sensor (MAPS) and AC Coupled Low Gain Avalanche Diode (AC-LGAD) technologies stand out of several advanced technology options for the EIC silicon vertex and tracking detector subsystems. The MAPS technology has advanced features of low material budget, low power consumption, good radiation resistance and fine spatial resolution. The AC-LGAD technology can achieve fast timing resolution. Latest studies and progress of the EIC silicon vertex and tracking detector conceptual design, performance validations in simulation and ongoing MAPS and AC-LGAD R&D will be shown. Furthermore, schedule and plan of the EIC project detector development will be discussed as well.

46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND ↗
DOE OSTI2023

Vegetation-induced asymmetric diurnal land surface temperatures changes across global climate zones

Unprecedented global vegetation greening during past decades is well known to affect annual and seasonal land surface temperatures (LST). However, the impact of observed vegetation cover change on diurnal LST across global climatic zones is not well understood. In this study, using global climatic time-series datasets, we investigated the long-term growing season daytime and nighttime LST changes globally and explored associated dominant contributors including vegetation and climate factors including air temperature, precipitation, and solar radiation. Results revealed asymmetric growing season mean daytime and nighttime LST warming (0.16 °C/10a and 0.30 °C/10a, respectively) globally from 2003 to 2020, as a result, the diurnal LST range (DLSTR) declined at 0.14 °C/10a. The sensitivity analysis indicated the LST response to changes in LAI, precipitation, and SSRD mainly concentrated during daytime instead of nighttime, however, which showed comparable sensitivities for air temperature. Combining the sensitivities results and the observed LAI and climate trends, we found rising air temperature contributes to 0.24 ± 0.11 °C/10a global daytime LST warming and 0.16 ± 0.07 °C/10a nighttime LST warming, turns to be the dominant contributor to the LST changes. Increased LAI cooled global daytime LST (–0.068 ± 0.096 °C/10a) while warmed nighttime LST (0.064 ± 0.046 °C/10a); hence LAI dominates declines in DLSTR trends (–0.12 ± 0.08 °C/10a), despite some daynight process variations across climate zones. In Boreal regions, reduced DLSTR was due to nighttime warming from LAI increases. In other climatic zones, daytime cooling, and DLSTR decline, was induced by increased LAI. Biophysically, the pathway from air temperature heats the surface through sensible heat and increased downward longwave radiation during day and night, while the pathway from LAI cools the surface by enhancing energy redistribution into latent heat rather than sensible heat during the daytime. These empirical findings of diverse asymmetric responses could help calibrate and improve biophysical models of diurnal surface temperature feedback in response to vegetation cover changes in different climate zones.

54 ENVIRONMENTAL SCIENCES↗