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Rodriguez, Miguel

Publications and source records attributed to Rodriguez, Miguel.

35 records · Page 2
All sourcesNASA NTRSDOE OSTI
DOE OSTI2020

Silencing Folylpolyglutamate Synthetase1 (FPGS1) in Switchgrass (Panicum virgatum L.) Improves Lignocellulosic Biofuel Production

Switchgrass (Panicum virgatum L.) is a lignocellulosic perennial grass with great potential in bioenergy field. Lignocellulosic bioenergy crops are mostly resistant to cell wall deconstruction, and therefore yield suboptimal levels of biofuel. The one-carbon pathway (also known as C1 metabolism) is critical for polymer methylation, including that of lignin and hemicelluloses in cell walls. Folylpolyglutamate synthetase (FPGS) catalyzes a biochemical reaction that leads to the formation of folylpolyglutamate, an important cofactor for many enzymes in the C1 pathway. In this study, the putatively novel switchgrass PvFPGS1 gene was identified and its functional role in cell wall composition and biofuel production was examined by RNAi knockdown analysis. The PvFPGS1-downregulated plants were analyzed in the field over three growing seasons. Transgenic plants with the highest reduction in PvFPGS1 expression grew slower and produced lower end-of-season biomass. Transgenic plants with low-to-moderate reduction in PvFPGS1 transcript levels produced equivalent biomass as controls. There were no significant differences observed for lignin content and syringyl/guaiacyl lignin monomer ratio in the low-to-moderately reduced PvFPGS1 transgenic lines compared with the controls. Similarly, sugar release efficiency was also not significantly different in these transgenic lines compared with the control lines. However, transgenic plants produced up to 18% more ethanol while maintaining congruent growth and biomass as non-transgenic controls. Severity of rust disease among transgenic and control lines were not different during the time course of the field experiments. Altogether, the unchanged lignin content and composition in the low-to-moderate PvFPGS1-downregulated lines may suggest that partial downregulation of PvFPGS1 expression did not impact lignin biosynthesis in switchgrass. In conclusion, the manipulation of PvFPGS1 expression in bioenergy crops may be useful to increase biofuel potential with no growth penalty or increased susceptibility to rust in feedstock.

59 BASIC BIOLOGICAL SCIENCES↗
DOE OSTI2020

Setting operating points for circuits in an integrated circuit chip using an integrated voltage regulator power loss model

An apparatus includes an integrated circuit chip with a set of circuits having two or more subsets of circuits; an external voltage regulator separate from the integrated circuit chip; two or more integrated voltage regulators on the integrated circuit chip that each provide an input voltage to a respective subset of the circuits; and a controller. The controller determines, using an integrated voltage regulator power loss model, an electrical power loss for the integrated voltage regulators for a first combination of operating points for the subsets of the circuits. The controller then determines, based on the electrical power loss, a second combination of operating points for the subsets of the circuits that includes an adjustment to an operating point for at least one of the subsets of the circuits that compensates for an electrical power loss of the corresponding integrated voltage regulator. The controller sets an operating point of each of the subsets of the circuits based on the second combination of operating points.

42 ENGINEERING↗
DOE OSTI2018

Microbial electrolysis using aqueous fractions derived from Tail-Gas Recycle Pyrolysis of willow and guayule

Here, this study investigated microbial electrolysis of two aqueous phase waste products derived from guayule and willow generated from Tail Gas Recycle Pyrolysis (TGRP). The highest average current density achieved was 5.0 ± 0.7 A/m 2 and 1.8 ± 0.2 A/m 2 for willow and guayule respectively. Average hydrogen productivity was 5.0 ± 1.0 L/L-day from willow and 1.5 ± 0.2 L/L-day for guayule. Willow also generated higher coulombic efficiency, anode conversion efficiency, and hydrogen recovery than guayule at most organic loading conditions. Compounds investigated exceeded 80% degradation, which included organic acids, sugar derivatives, and phenolics. Mass spectrometric analysis demonstrated the accumulation of a long chain amine not present in either substrate before treatment, and the persistence of several peptide residues resulting from the TGRP process. Lastly, new biorefineries may one day capitalize on this otherwise discarded byproduct of TGRP, further improving the potential applications and value of microbial electrolysis towards energy production.

09 BIOMASS FUELS↗
DOE OSTI2018

Reduced-Order Aggregate Model for Parallel-Connected Single-Phase Inverters

This paper outlines a reduced-order aggregate dynamical model for parallel-connected single-phase grid-connected inverters. For each inverter, we place no restrictions on the converter topology and merely assume that the ac-side switch-averaged voltage can be controlled via pulsewidth modulation. The ac output of each inverter interfaces through an LCL filter to the grid. The closed-loop system contains a phase locked loop for grid synchronization, and real- and reactive-power control are realized with inner and outer PI current- and power-control loops. We derive a necessary and sufficient set of parametric relationships to ensure that a reduced-order aggregated state-space model for an arbitrary number of such paralleled inverters has the same model order and structure as any single inverter. We also present reduced-order models for the settings where the real- and reactive-power setpoints are different and where the inverters have different power ratings. We anticipate the proposed model being useful in analyzing the dynamics of large collections of parallel-connected inverters with minimal computational complexity. The aggregate model is validated against measurements obtained from a multi-inverter experimental setup consisting of three 750-VA paralleled grid-connected inverters, hence establishing robustness of the analytical result to parametric variations seen in practice.

24 POWER TRANSMISSION AND DISTRIBUTION↗
DOE OSTI2018

Hydrogen production from pine-derived catalytic pyrolysis aqueous phase via microbial electrolysis

In this paper, microbial electrolysis of an aqueous phase generated from catalytic pyrolysis of pine sawdust was investigated for renewable hydrogen production. The microbial electrolysis cell (MEC) performance was investigated at an organic loading rate ranging from 2 to 50 g/L-day. A maximum hydrogen productivity of 5.8 ± 0.18 L/L-day was obtained, however, the productivity increased linearly only up to a loading rate of 10 g/L-day. The highest current density achieved was 6.8 ± 0.1 A/m 2 . The efficiency of conversion of the substrate to current in the anode decreased with increasing loading, but the initial maximum Coulombic efficiency was 98 ± 0.04%. The cathode efficiency, on the other hand, increased with loading up to a maximum of 89 ± 1.4%. Total hydrogen recovery was relatively constant for most runs at 30%, which is equivalent to an yield of 0.6 moL H 2 /mole COD, except at the highest loading rate. The operation of the MEC under batch mode, however, resulted in a higher hydrogen recovery of 63 ± 4%. The conversion of a wide range of compounds, including carboxylic acids, anhydrosugars, furanic and phenolic compounds present in the aqueous phase is reported. Finally, the results demonstrate potential for hydrogen production from a waste stream which can improve the total biofuel or energy yield of the biorefinery.

08 HYDROGEN↗
DOE OSTI2018

Decentralized Interleaving of Parallel-Connected Buck Converters

We present a decentralized control strategy that yields switch interleaving for parallel-connected dc-dc buck converters. Compared to state-of-the-art methods that are distributed at best, the proposed architecture requires no communication, and hence, presents a variety of advantages with regard to reliability, modularity, and cost. The method is based on the digital implementation of the dynamics of a Lienard-type oscillator circuit as the controller for the converters. Each controller only requires the locally measured output current to synthesize the pulsewidth modulation (PWM) carrier waveform. The intrinsic electrical coupling between converters drives the nonlinear-oscillator-based controllers to converge to an interleaved state with uniform phase spacing across PWM carriers, independent of the number of converters, the load, and initial conditions. We provide analytical guarantees for existence and stability of the interleaved state as well as extensive hardware results for a system of five 120 W 48 V-to-12 V dc-dc buck converters that demonstrate convergence to the interleaved state in the face of a variety of large-signal disturbances.

24 POWER TRANSMISSION AND DISTRIBUTION↗
DOE OSTI2018

Sugar release and growth of biofuel crops are improved by downregulation of pectin biosynthesis

Cell walls in crops and trees have been engineered for production of biofuels and commodity chemicals, but engineered varieties often fail multi-year field trials and are not commercialized. In this paper, we engineered reduced expression of a pectin biosynthesis gene (Galacturonosyltransferase 4, GAUT4) in switchgrass and poplar, and find that this improves biomass yields and sugar release from biomass processing. Both traits were maintained in a 3-year field trial of GAUT4-knockdown switchgrass, with up to sevenfold increased saccharification and ethanol production and sixfold increased biomass yield compared with control plants. We show that GAUT4 is an α-1,4-galacturonosyltransferase that synthesizes homogalacturonan (HG). Downregulation of GAUT4 reduces HG and rhamnogalacturonan II (RGII), reduces wall calcium and boron, and increases extractability of cell wall sugars. Finally, decreased recalcitrance in biomass processing and increased growth are likely due to reduced HG and RGII cross-linking in the cell wall.

09 BIOMASS FUELS↗
DOE OSTI2017

Extended Pulse-Powered Humidity-Freeze Cycling for Testing Module-Level Power Electronics

An EMI suppression capacitor (polypropylene film type) failed by 'popcorning' due to vapor outgassing in pulse powered humidity-freeze cycles. No shorts or shunts could be detected despite mildly corroded metallization visible in the failed capacitor. Humidity-freeze cycling is optimized to break into moisture barriers. However, further studies will be required on additional module level power electronic (MLPE) devices to optimize the stress testing for condensation to precipitate any weakness to short circuiting and other humidity/bias failure modes.

14 SOLAR ENERGY↗
DOE OSTI2017

Lignin Exhibits Recalcitrance-Associated Features Following the Consolidated Bioprocessing of Populus trichocarpa Natural Variants

Because cellulosic ethanol production remains cost-prohibitive„ advances in consolidated bioprocessing (CBP) have been directed towards lifting this restriction. CBP reduces the need for added enzymes and can potentially slash ethanol production costs through process integration. Clostridium thermocellum, a CBP microorganism, organizes its enzymes in a multi-enzyme complex - a stark contrast to fungal enzymes. Nonetheless, recalcitrance may limit the extent of biomass deconstruction. Here in this study, six Populus were treated with C. thermocellum (ATCC 27405) and characterized to determine structural changes that resulted from CBP. The 2D HSQC NMR spectra of lignin-enriched residues revealed that higher S/G ratio (2.6) and fewer carbon-carbon interunit linkages (generally 2–5%) were present in the top performing poplar. Furthermore, cellulose degree of polymerization data suggests that C. thermocellum likely circumvents long chain cellulose, while cellulose crystallinity and hemicellulose molecular weight data do not provide a direct indication of features connected to recalcitrance. Hence, C. thermocellum is similarly impacted by the proposed lignin properties that negatively impact biomass deconstruction using fungal enzymes.

59 BASIC BIOLOGICAL SCIENCES↗
DOE OSTI2017

Insights of biomass recalcitrance in natural Populus trichocarpa variants for biomass conversion

Populus has been investigated as a promising biomass feedstock for alternative fuels and chemicals. Physicochemical characteristics and genomic information of biomass feedstocks are among the essential information that can help not only advance our understanding of biomass recalcitrance but also in its efficient utilization. Here in this paper, the recalcitrance of natural Populus variants was elucidated in three aspects: (1) sugar release, (2) physicochemical properties, and (3) relative variation of gene expression within natural poplar variants. The sugar release performance of natural Populus variants was evaluated with their correlation with biomass recalcitrance-related characteristics. Among the physicochemical properties of poplar, the lignin content, lignin molecular weight, lignin S/G ratio, and cellulose accessibility were found to correlate with sugar release. The results demonstrated that the lignin content was negatively correlated with sugar release, whereas the lignin molecular weight, lignin S/G ratio, and cellulose accessibility were positively associated with poplar sugar release. The trend of differential gene expression of each variant also supports the characterization results and their effects on biomass conversion.

09 BIOMASS FUELS↗
DOE OSTI2017

Comparison of Virtual Oscillator and Droop Control: Preprint

Virtual oscillator control and droop control are two techniques that can be used to ensure synchronization and power sharing of parallel inverters in islanded operation. VOC relies on the implementation of non-linear Van der Pol oscillator equations in the control system of the inverter, acting upon the time-domain instantaneous inverter current and terminal voltage. On the other hand, DC explicitly computes active and reactive power produced by the inverter and relies on limited bandwidth low-pass filters. Even though both methods can be engineered to produce the same steady-state characteristics, their dynamic performances are significantly different. This paper presents analytical and experimental results that aim to compare both methods. It is shown that VOC is inherently faster and enables minimizing the circulating currents. The results are verified using three 120V, 1kW inverters.

24 POWER TRANSMISSION AND DISTRIBUTION↗
DOE OSTI2017

Decentralized Interleaving of Paralleled Dc-Dc Buck Converters: Preprint

We present a decentralized control strategy that yields switch interleaving among parallel connected dc-dc buck converters without communication. The proposed method is based on the digital implementation of the dynamics of a nonlinear oscillator circuit as the controller. Each controller is fully decentralized, i.e., it only requires the locally measured output current to synthesize the pulse width modulation (PWM) carrier waveform. By virtue of the intrinsic electrical coupling between converters, the nonlinear oscillator-based controllers converge to an interleaved state with uniform phase-spacing across PWM carriers. To the knowledge of the authors, this work represents the first fully decentralized strategy for switch interleaving of paralleled dc-dc buck converters.

14 SOLAR ENERGY↗
DOE OSTI2017

Decentralized Interleaving of Paralleled Dc-Dc Buck Converters

We present a decentralized control strategy that yields switch interleaving among parallel-connected dc-dc buck converters. The proposed method is based on the digital implementation of the dynamics of a nonlinear oscillator circuit as the controller. Each controller is fully decentralized, i.e., it only requires the locally measured output current to synthesize the pulse width modulation (PWM) carrier waveform and no communication between different controllers is needed. By virtue of the intrinsic electrical coupling between converters, the nonlinear oscillator-based controllers converge to an interleaved state with uniform phase-spacing across PWM carriers. To the knowledge of the authors, this work presents the first fully decentralized strategy for switch interleaving in paralleled dc-dc buck converters.

14 SOLAR ENERGY↗
DOE OSTI2017

Generation and Characterization of Acid Tolerant Fibrobacter succinogenes S85

Microorganisms are key components for plant biomass breakdown within rumen environments. Fibrobacter succinogenes have been identified as being active and dominant cellulolytic members of the rumen. In this study, F. succinogenes type strain S85 was adapted for steady state growth in continuous culture at pH 5.75 and confirmed to grow in the range of pH 5.60–5.65, which is lower than has been reported previously. Wild type and acid tolerant strains digested corn stover with equal efficiency in batch culture at low pH. RNA-seq analysis revealed 268 and 829 genes were differentially expressed at pH 6.10 and 5.65 compared to pH 6.70, respectively. Resequencing analysis identified seven single nucleotide polymorphisms (SNPs) in the suf D, yid E, xyl E, rlm M, msc L and dos C genes of acid tolerant strains. Due to the absence of a F. succinogenes genetic system, homologues in Escherichia coli were mutated and complemented and the resulting strains were assayed for acid survival. Complementation with wild-type or acid tolerant F. succinogenes suf D restored E. coli wild-type levels of acid tolerance, suggesting a possible role in acid homeostasis. Here, recent genetic engineering developments need to be adapted and applied in F. succinogenes to further our understanding of this bacterium.

59 BASIC BIOLOGICAL SCIENCES↗