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It is increasingly common to see spacecraft equipped with cameras for the purpose of navigation. Images are either sent to Earth or processed autonomously on-board to provide information about the vehicle’s position, velocity, and/or attitude. These can be images of stars or celestial bodies for absolute navigation, or images of another spacecraft for relative navigation. While monocular cameras do not provide range information, the images they capture can be processed to determine bearing vectors to target objects within the camera’s field of view. For a camera to be effective in navigation, it must be carefully calibrated and aligned. This involves accurately modeling the optical effects that govern the projection of line-of-sight directions onto the camera’s pixels and determining the camera’s orientation relative to the spacecraft’s reference frame. Engineers at Johnson Space Center’s Electro-Optics Lab regularly perform camera inspection, calibration, and alignment metrology. This was done for the Orion Optical Navigation (OpNav) Camera, the Orion Docking Camera (DCAM), and for numerous cameras belonging to commercial partners. The nature of optical navigation means that cameras must be well-calibrated and their attitude well understood to provide high accuracy bearing measurements to the navigation filter. The stringent accuracy requirements for Orion could not have been met using traditional checkerboard camera calibration or by simply relying on design drawings. This paper details the hardware, software, techniques, and algorithms used by the EOL team to achieve this level of accuracy.