Recombinant hydrogen-producing cyanobacterium and uses thereof
A recombinant cyanobacterium comprising an oxygen-tolerant, hydrogen-evolving hydrogenase, kit, and methods of use.
59 BASIC BIOLOGICAL SCIENCES↗
Engineering topics
Smith, Hamilton O
Publications and source records attributed to Smith, Hamilton O.
Method for in vitro recombination
The present invention relates to an in vitro method, using isolated protein reagents, for joining two double-stranded (ds) DNA molecules of interest, wherein the distal region of the first DNA molecule and the proximal region of the second DNA molecule share a region of sequence identity. The method allows the joining of a number of DNA fragments, in a predetermined order and orientation, without the use of restriction enzymes. It can be used, e.g., to join synthetically produced sub-fragments of a gene or genome of interest.
59 BASIC BIOLOGICAL SCIENCES↗