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DOE OSTI · 1084234

Method for in vitro recombination

Gibson, Daniel Glenn·Smith, Hamilton O
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Abstract

The present invention relates to an in vitro method, using isolated protein reagents, for joining two double-stranded (ds) DNA molecules of interest, wherein the distal region of the first DNA molecule and the proximal region of the second DNA molecule share a region of sequence identity. The method allows the joining of a number of DNA fragments, in a predetermined order and orientation, without the use of restriction enzymes. It can be used, e.g., to join synthetically produced sub-fragments of a gene or genome of interest.

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BibTeXRIS

Gibson, Daniel Glenn, Smith, Hamilton O. 2013-05-07. Method for in vitro recombination. https://www.osti.gov/biblio/1084234

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