An algorithm for neurite outgrowth reconstruction

Weaver, Christina M.; Pinezich, John D.; Lindquist, W. Brent; Vazquez, Marcelo E.

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NASA NTRS · 20040087728

An algorithm for neurite outgrowth reconstruction

Weaver, Christina M.·Pinezich, John D.·Lindquist, W. Brent·Vazquez, Marcelo E.

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Abstract

We present a numerical method which provides the ability to analyze digitized microscope images of retinal explants and quantify neurite outgrowth. Few parameters are required as input and limited user interaction is necessary to process an entire experiment of images. This eliminates fatigue related errors and user-related bias common to manual analysis. The method does not rely on stained images and handles images of variable quality. The algorithm is used to determine time and dose dependent, in vitro, neurotoxic effects of 1 GeV per nucleon iron particles in retinal explants. No neurotoxic effects are detected until 72 h after exposure; at 72 h, significant reductions of neurite outgrowth occurred at doses higher than 10 cGy.

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NASA Program Biomedical Research and Countermeasures NASA Discipline Radiation Health Non-NASA Center Algorithms Neurites/drug effects/physiology Dose-Response Relationship, Drug Animals Support, U.S. Gov't, Non-P.H.S Chick Embryo Image Processing, Computer-Assisted/instrumentation/methods Support, Non-U.S. Gov't Cells, Cultured Cell Differentiation/physiology Comparative Study

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Weaver, Christina M., Pinezich, John D., Lindquist, W. Brent, Vazquez, Marcelo E.. 2003-04-15. An algorithm for neurite outgrowth reconstruction. https://ntrs.nasa.gov/citations/20040087728

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